Protein Alignment alpha-Cat and VCL

DIOPT Version :10

• Sequence 1: NP_524219.1
• Gene: alpha-Cat / 40517
• FlyBaseID: FBgn0010215
• Length: 917
• Species: Drosophila melanogaster
• Sequence 2: NP_054706.1
• Gene: VCL / 7414
• HGNCID: 12665
• Length: 1134
• Species: Homo sapiens
• Alignment Length: 1151
• Identity: 243/1151 - (21%)
• Similarity: 428/1151 - (37%)
• Gaps: 330/1151 - (28%)

  Fly    29 TMSVEKTLEPLVLQVTTLV--NTKGPSKKKKGKS-KRASALVAAVEKATENFIQKGEQIAYENPD 90
            |.::|..|||:..|::.||  :.:|   :..||: ...:|.||||:.|..|.::.|::......|
Human     6 TRTIESILEPVAQQISHLVIMHEEG---EVDGKAIPDLTAPVAAVQAAVSNLVRVGKETVQTTED 67

  Fly    91 --ITQEMLTAVDEVKKTGDAMSIAAREFSEDPCSSLKRGNMVRAARNLLSAVTRLLILADMVDVH 153
              :.::|..|..:|:.....:..||:....||.|...|..::..:|.:||..:.||:..|..:|.
Human    68 QILKRDMPPAFIKVENACTKLVQAAQMLQSDPYSVPARDYLIDGSRGILSGTSDLLLTFDEAEVR 132

  Fly   154 LLLKSLHIVEDDLNKLKNASSQDELMDNMRQFGRNAGELIKQAAKRQQELKDPQLRDDLAAARAM 218
            .:::....:.:.|...:...:.::|:...:..|....::.|...:|||||...:.|..|..:...
Human   133 KIIRVCKGILEYLTVAEVVETMEDLVTYTKNLGPGMTKMAKMIDERQQELTHQEHRVMLVNSMNT 197

  Fly   219 LKKHSTMLLTASKVYV-----RHPELDLAKVNRDFILKQVCDAVNTI------------------ 260
            :|:...:|::|.|::|     ::..::.|..||:|.::::...:|.|
Human   198 VKELLPVLISAMKIFVTTKNSKNQGIEEALKNRNFTVEKMSAEINEIIRVLQLTSWDEDAWASKD 262

  Fly   261 ------------SDVAQGK------SSQPTDIYSGA-----------GELAAALD---------- 286
                        |.:.|.|      |:.|.|....|           |||.|..:
Human   263 TEAMKRALASIDSKLNQAKGWLRDPSASPGDAGEQAIRQILDEAGKVGELCAGKERREILGTCKM 327

  Fly   287 ---------------------------DFDEGI------------VMDPMTYSEKRSRQLL---- 308
                                       ...:|:            .::.||.|::...:.:
Human   328 LGQMTDQVADLRARGQGSSPVAMQKAQQVSQGLDVLTAKVENAARKLEAMTNSKQSIAKKIDAAQ 392

  Fly   309 ------------EERLESIISAAALMADADCTRDERRE---RIVAECNAVRQALQDL-------- 350
                        ||::...::.|..:|:. |...:.|:   |.:.|.:|:...|.||
Human   393 NWLADPNGGPEGEEQIRGALAEARKIAEL-CDDPKERDDILRSLGEISALTSKLADLRRQGKGDS 456

  Fly   351 ---------LSEYMSNMSQKDN---------------------------SP-----GLSRA---- 370
                     ::..:.|:..|.|                           :|     |:.:|
Human   457 PEARALAKQVATALQNLQTKTNRAVANSRPAKAAVHLEGKIEQAQRWIDNPTVDDRGVGQAAIRG 521

  Fly   371 --------------------------IDQMCRK-------------------------TRDLRRQ 384
                                      :||:..:                         .:||:.:
Human   522 LVAEGHRLANVMMGPYRQDLLAKCDRVDQLTAQLADLAARGEGESPQARALASQLQDSLKDLKAR 586

  Fly   385 LRKAVVDHVSDSFLETTTPLLDLIEAAKS-----GNEKKVREKSEIFTKHAEKLVEVANLVCSMS 444
            :::|:...|||.|.:||||:..|..||.:     ..|:...|::..|..|:.||...|....::.
Human   587 MQEAMTQEVSDVFSDTTTPIKLLAVAATAPPDAPNREEVFDERAANFENHSGKLGATAEKAAAVG 651

  Fly   445 N-NEDGVKMVRYAAAQIESLCPQVINAASILTVRPNSKVAQENMTTYRQAWEVQVRILTEAVDDI 508
            . |:..|:.::.:......|.|||::||.||...|.::.|.|:..|.:..|...|..:|..||:.
Human   652 TANKSTVEGIQASVKTARELTPQVVSAARILLRNPGNQAAYEHFETMKNQWIDNVEKMTGLVDEA 716

  Fly   509 TTIDDFLAVSENHILEDVNKCVMALQVGDARDLRATAGAIQGRSSRVCNVVEAEMDNYEPCIYTK 573
            ......|..||..|.:|::||.:|:.....:.|.|.|.:|..|::|:..|.:.|::|.|    ..
Human   717 IDTKSLLDASEEAIKKDLDKCKVAMANIQPQMLVAGATSIARRANRILLVAKREVENSE----DP 777

  Fly   574 RVLEAVKVLRDQVMMKFDQRVGAAVGALSNNSNKDVDENDFIDASRLVYDGVREIRRA------- 631
            :..||||...|::.......|..|.....|.|:..: :..|:|:...:...|.::|.|
Human   778 KFREAVKAASDELSKTISPMVMDAKAVAGNISDPGL-QKSFLDSGYRILGAVAKVREAFQPQEPD 841

  Fly   632 -------VLMNRSSEDL---------------------DTDTEF----------EPV----EDLT 654
                   :...|.:::|                     :.|.||          :|:    ..|.
Human   842 FPPPPPDLEQLRLTDELAPPKPPLPEGEVPPPRPPPPEEKDEEFPEQKAGEVINQPMMMAARQLH 906

  Fly   655 LETRSRSS------AHTGDQTVDEY-----------PDISGICTAREAMRKMTEEDKQKIAQQVE 702
            .|.|..||      |..|...|.|.           ||:.........:....:...|.|....:
Human   907 DEARKWSSKPGIPAAEVGIGVVAEADAADAAGFPVPPDMEDDYEPELLLMPSNQPVNQPILAAAQ 971

  Fly   703 LFRREKLTFDSEVAKWDDTGNDIIFLAKHMCMIMMEMTDFTRGRGPLKTTMDVINAAKKISEAGT 767
            ...|       |..||...|||||..||.|.::|.||:...||....|..:  |..||.|::|..
Human   972 SLHR-------EATKWSSKGNDIIAAAKRMALLMAEMSRLVRGGSGTKRAL--IQCAKDIAKASD 1027

  Fly   768 KLDKLTREIAEQCPESSTKKDLLAYLQRIALYCHQIQITSKVKADV---QNISGELIVSGLDSAT 829
            ::.:|.:|:|:||.:...:.:||...:||.....|::|.|.|||.:   .|||.|    ..:.||
Human  1028 EVTRLAKEVAKQCTDKRIRTNLLQVCERIPTISTQLKILSTVKATMLGRTNISDE----ESEQAT 1088

  Fly   830 S-LIQAAKNLMNAVVLTVKYSYVASTKYTRQGTVSSPIVVWKMKAP 874
            . |:..|:|||.:|..||:.:..||.|..   |.:...:.|..|.|
Human  1089 EMLVHNAQNLMQSVKETVREAEAASIKIR---TDAGFTLRWVRKTP 1131

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
alpha-Cat	NP_524219.1	Vinculin	26..877	CDD:460040	243/1151 (21%)
VCL	NP_054706.1	N-terminal globular head. /evidence=ECO:0000269|PubMed:20484056	1..835		164/837 (20%)
		Vinculin	3..1134	CDD:460040	243/1151 (21%)
		Talin-interaction. /evidence=ECO:0000250|UniProtKB:P12003	168..208		10/39 (26%)
		3 X 112 AA tandem repeats. /evidence=ECO:0000255	259..589		37/330 (11%)
		Interaction with ACTN4. /evidence=ECO:0000269|PubMed:15988023, ECO:0007744|PDB:1YDI	741..764		6/22 (27%)
		Linker (Pro-rich). /evidence=ECO:0000269|PubMed:20484056	836..878		2/41 (5%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	857..887		4/29 (14%)
		C-terminal tail. /evidence=ECO:0000269|PubMed:20484056	879..1134		76/269 (28%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1003..1046		13/44 (30%)
		Facilitates phospholipid membrane insertion. /evidence=ECO:0000250|UniProtKB:Q64727	1120..1134		3/12 (25%)