Protein Alignment Als2 and als2

DIOPT Version :9

• Sequence 1: NP_649347.1
• Gene: Als2 / 40410
• FlyBaseID: FBgn0037116
• Length: 1486
• Species: Drosophila melanogaster
• Sequence 2: XP_004917812.2
• Gene: als2 / 779575
• XenbaseID: XB-GENE-922619
• Length: 1640
• Species: Xenopus tropicalis
• Alignment Length: 1670
• Identity: 397/1670 - (23%)
• Similarity: 634/1670 - (37%)
• Gaps: 509/1670 - (30%)

  Fly    34 GLPPLQRFPASRI-CSNAGGELLLLTTDHALYSAKLQANREHMDLQL-----LRTDVVDMDFCSG 92
            |:...:..|.||. .|:.|..:..:..:|..:||.....:|.....|     |.||..  .||..
 Frog   258 GVMLAESHPGSRSNQSSFGLSVKSVKKEHISHSAMTHEEKETSQQYLESDTELGTDAA--SFCET 320

  Fly    93 SQELFVVLTNGSV--QRQATGSGRDVGHPHAWQTLGFDPLELHAEGVRIRRVCCSAQGVVFVGAS 155
            ........:||::  ..|:....:| |.....|....:|||..:|         :|.....:..|
 Frog   321 ETSQIFGKSNGTIPAPSQSEQYLKD-GMAEVTQLQEQNPLENVSE---------TAGSSTAIATS 375

  Fly   156 GETYVMGSCGEVFKAEQQPRHMRLYEEG----KELLDLAAGNEHFVMLVAPYNLADDALQLSVAS 216
            |...:|.||......    |.....|.|    |::::..:|           :|.|..:|..|:|
 Frog   376 GLNSLMVSCASAVSV----RVAATCEAGALSLKKVMNFYSG-----------SLCDPGVQQGVSS 425

  Fly   217 AKE----EPEDERASVKSISSGHS--------ERSVAANTR----HLLHQ--------------- 250
            :.:    :..:|:..::|:....|        |.:.|.|.|    .||.|
 Frog   426 SAQDGLKDSREEQVKLESMQGKKSSSLVDIREEEADAGNRRLSLPGLLSQVSPRLLRKVGRARMR 490

  Fly   251 ---------GYA-----LLHTQLFTFGASNNGLLGSGDHIRRANVMRLQKLDSMGVCSIAAGLEH 301
                     |.|     .|.|:::::|....|.||.||.:.|...:.::.:|...|..:|||..|
 Frog   491 TVALTPTNSGEADVLLPSLKTEVWSWGRGKEGQLGHGDVLPRLQPLCVRSMDGKEVIQLAAGSHH 555

  Fly   302 TVARTLDGRLYHWGLNNHSQLGEDVSSPMEITITENTAALP-----IEQNSALEATCGDYHTLLL 361
            ::|.|...::|.||.|:..|||. :.||         ..:|     .|.:.......|..|:|.|
 Frog   556 SLALTARSQVYSWGSNSCGQLGH-LQSP---------TTMPRLVKVYESDGVWSVAAGRDHSLFL 610

  Fly   362 NASGQIH---SLQPAPPMRHLQQSSTY-----AQTLLQL----QLGAAWPRQLRLLMCS------ 408
                 :|   ..|||.....||..:.:     :|..::|    :||         .:||
 Frog   611 -----VHKKEEFQPAVYYCGLQTVNNWSGPDPSQFPIELNECGKLG---------YICSISAGAD 661

  Fly   409 ----------GGY--TLQNQRQFQRQYHYYLSHLQSQLQLLLKHRQAVQTLEIWQRQSALEPLSA 461
                      .||  :|......:|:::.:||.::|   |:|:....::.|   ...:||:.|..
 Frog   662 CCLALVDQNMMGYIGSLHELADTERRFYNHLSDMKS---LILRPLLGLENL---GSATALQQLQN 720

  Fly   462 LGPLLINWERILCLLV-----ATLHSLEGFYRADFVQPADLLFICHYKEYIDLF-DGYTKAYCDV 520
            :......    ||.|:     :.:|.|.|      .:.|..|.|.   |:..|| |.||: ||..
 Frog   721 MATKFSK----LCYLIGQHSTSLVHFLRG------AKDARSLSIL---EHSSLFLDSYTE-YCTS 771

  Fly   521 FS----VNGFGEAVVAITGLSSPLAEL--------------NEESY-----VTRLFQQPFSIYQL 562
            ||    :.||       |.||....|.              .|||.     :...|..|.|..|
 Frog   772 FSNFLVMGGF-------TVLSKQAMEFFSKNPEHLQIFSEGTEESISIAEKLNSFFFSPMSRLQ- 828

  Fly   563 FVQFMELLVR-------TQSEYGEHRVAWSEFARHSCISQELAVNTK--------DFWSSNDRNP 612
              ::..||::       :..||.:.:.:.|.:.     |..|.||.|        .||.:
 Frog   829 --EYARLLLKLAMCFEVSSPEYKKLQDSSSSYD-----SLALLVNRKKKDAEYTLGFWKT----- 881

  Fly   613 RIVQFRGR--------HRRVILTSALVPLKLVTSGISRSSNSFILFSDFLCQVSGNSLYSYPLTT 669
                |.|:        .||:|..|:...|.|..:| ..|.|.||||:|.|.....::.:.:||:|
 Frog   882 ----FPGKMTDSLRKPDRRLICDSSNRGLSLQNAG-RFSVNWFILFNDALVHAQFSTHHIFPLST 941

  Fly   670 LWVWTEGD------SLRLTTPEKSFLVSTRSQEMRKVWL-------DQLQSSIIASLGKPLGS-- 719
            :||....:      .|::||||:...:.:.||:.:..||       ||....|...|.:..||
 Frog   942 VWVEPLSEEVNGVNGLKITTPEEQLTLVSSSQQEKAKWLWAISQAVDQALKGITEPLAQSGGSVH 1006

  Fly   720 --PVPSYRSTGYEFSREHPKFSRVKACGTWRKGVLHGNCYLEYPDGSVYCGELQHGIIEGFGKMV 782
              ..|..|:..|.|.:: .:....|..|.|..|..||...|.:|||.:|.|..::|:.:|:|:..
 Frog  1007 RQEPPLSRTAKYTFYKD-LRLKDAKYEGRWLSGKPHGRGTLSWPDGRMYSGTFRNGLEDGYGECT 1070

  Fly   783 IPTTGL-----YVGNFKGGRFHGHGVYEMHCKDSPESEVYEGNFCEGLFHGHGVMR------NNR 836
            ||...|     |.|::|.|:..|||.|.....|     ||||.|.:.:.||||::|      |:.
 Frog  1071 IPNKTLNKNDRYAGHWKDGKMCGHGTYWYAIGD-----VYEGCFQDNMRHGHGLLRSGKLTSNSP 1130

  Fly   837 YIYVGEYQANARSGYGVIEDLVSGDKYMGMFADNKRSGIGSCITNRGDYFEGSFSGDDLTGSGVA 901
            .|::||:..:.:.||||.:|:..|.|:|||:.|:.|.|.|..:|..|.|:||:|:.:.:.|.||.
 Frog  1131 SIFIGEWVMDKKCGYGVFDDITRGQKFMGMWQDDARQGNGLVVTQFGLYYEGAFNLNRMMGCGVL 1195

  Fly   902 VFENDYYYEGEL----TLLGPNGRGEYYMPSGDACGG--SGAMGTGEFDDTCELIGNKMFGQLSG 960
            :.|:|..||||.    ||   ||:|...|.:||...|  ||..|:|.              ::||
 Frog  1196 LSEDDTVYEGEFSDDWTL---NGKGVLTMSNGDCIEGVFSGEWGSGI--------------KVSG 1243

  Fly   961 TWDTVRIQAGELVLNRRFPKYPSSLGRQVVDHNRKWRSLFNNFESDLANCTASTSSSGGNQSAGT 1025
            .:....:..||....::.     .||..||..:.||:::|:.....|.   ......|..|.|
 Frog  1244 MFSKPNLFDGETEKEKKL-----KLGHLVVAADEKWKAIFDECWCHLG---CEIPGQGDTQKA-- 1298

  Fly  1026 LRKSSKPTLSTAQIWNCIAVYMSKQRAREGTKPGNYFNNILLSLPLPQKTSSPLAKARTTTSALS 1090
                          |:.|||.::..|.::...|.|
 Frog  1299 --------------WDNIAVALTTGRWQQKDSPEN------------------------------ 1319

  Fly  1091 KLQTEAASALDFLGFPPRRIQSQEALNSKPGGLQRADSLISMGHNTSRDLDNSSLASFQLDQSLM 1155
                            .||.|                              |.:|.|.:|
 Frog  1320 ----------------KRRSQ------------------------------NHTLESLEL----- 1333

  Fly  1156 NSTVNGDESSTFNESFSKLSHNNNNSISKHMN--NIDCSITSTTSTTSAVLDQVPSFGMALVLTE 1218
                                      |.:|::  |:|
 Frog  1334 --------------------------IPQHLSPFNMD---------------------------- 1344

  Fly  1219 QDVTSIRLYLEQAFKDRHHPLYVLNERIANCFHYSY-GYWKVKPTPILAKQAMREWESISRRIYR 1282
             ....|::||..|.:...|||..|.|.:...:..:| |   |.....|.::|:||.:|..:||::
 Frog  1345 -KYDKIKMYLINACETPVHPLGKLVETLVAVYRMTYVG---VGANRRLLEEAVREIKSYLKRIFQ 1405

  Fly  1283 FVRKMFPALPEE------------------LC-----QMEGSRE---VISHITLLYPLVLSEGIY 1321
            .||.:||.||||                  :|     .:..|.|   |::...||.|::|.. :|
 Frog  1406 LVRFLFPDLPEEGSMIQLLPAPKDSPEKIHICNGGTDSIPDSPEPGYVVTSSGLLLPVLLPR-LY 1469

  Fly  1322 STLFVLYANKYSRKDEMYRQNLNLAEKLKDQELVELMGHE--------SFL---HNVMLDPK--- 1372
            ..||:|||.::.:.:..|...:....|..|..|:..:|.:        :||   ..|:...|
 Frog  1470 PPLFMLYALEHEKDENAYWDCVLRLNKQPDIALLAFLGVQNKFWPTTINFLGETKQVLTSTKDSC 1534

  Fly  1373 FVESVQTLKELQEKFSPQDMLTVIQRSTQLLTEAYEHAMAANAAQLNADNMIPLTMLTMLRAAVP 1437
            |..:|:.|:::..:|:|.|.|.|||::.:.:|:..: .|.......:.|.:.||.:..:|||.:.
 Frog  1535 FASAVECLQQISTRFTPSDKLKVIQQTFEEITQEVQ-GMLKEDFLWSMDELFPLFLYVVLRARIR 1598

  Fly  1438 HLGAELALLDDLTGGPNFQAEMNGMAGYCYTTLKAAYEHVTSRAL 1482
            :||:|:.|::||. .|..|   :|..|..:|||||.|..:....|
 Frog  1599 NLGSEVHLIEDLM-DPYLQ---HGEQGIMFTTLKACYYQIQHEKL 1639

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Als2	NP_649347.1	RCC1	258..304	CDD:278826	13/45 (29%)
		RCC1_2	292..321	CDD:290274	11/28 (39%)
		RCC1	308..361	CDD:278826	14/57 (25%)
		PH-like	616..715	CDD:302622	35/119 (29%)
		VPS9	1373..1479	CDD:280383	34/105 (32%)
als2	XP_004917812.2	ATS1	<21..217	CDD:227511
		ATS1	<465..626	CDD:227511	42/175 (24%)
		RCC1	512..559	CDD:395335	13/46 (28%)
		RhoGEF	678..867	CDD:413383	51/223 (23%)
		PH_alsin	888..993	CDD:241423	31/105 (30%)
		PLN03185	1031..>1219	CDD:215619	74/195 (38%)
		VPS9	1537..1636	CDD:366977	33/103 (32%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	56	1.000	Domainoid score	I10853
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	297	1.000	Inparanoid score	I2674
OMA	1	1.010	-	-		QHG47592
OrthoDB	1	1.010	-	-		D25604at33208
OrthoFinder	1	1.000	-	-		FOG0002992
OrthoInspector	1	1.000	-	-		otm49092
Panther	1	1.100	-	-	LDO	PTHR46089
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5555
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	10.110