DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Als2 and Herc1

DIOPT Version :9

Sequence 1:	NP_649347.1	Gene:	Als2 / 40410	FlyBaseID:	FBgn0037116	Length:	1486	Species:	Drosophila melanogaster
Sequence 2:	XP_006511166.1	Gene:	Herc1 / 235439	MGIID:	2384589	Length:	4885	Species:	Mus musculus

Alignment Length:	1793	Identity:	330/1793 - (18%)
Similarity:	556/1793 - (31%)	Gaps:	655/1793 - (36%)

- Green bases have known domain annotations that are detailed below.

  Fly    91 SGSQELFVVLTNGSVQRQATGS-GR----DVGHPHAWQTLGFDPLELHAEGVRIRRVCCSAQGVV 150
            :|....||:.|:|||:....|| ||    |..:....:.|.|:|   |..   |::|..|.
Mouse   410 AGQYCTFVISTDGSVRACGKGSYGRLGLGDSNNQSTLKKLTFEP---HRS---IKKVSSSK---- 464

  Fly   151 FVGASGETYVMGSCGEVF---------------KAEQQPRHMRLYEEGKELLDLAAGNEHFVMLV 200
              |:.|.|....:.||||               ..::.|:.::...:||.::.::||..|...:.
Mouse   465 --GSDGHTLAFTTEGEVFSWGDGDYGKLGHGNSSTQKYPKLIQGPLQGKVVVCVSAGYRHSAAVT 527

  Fly   201 APYNLAD------DALQLSVASAKEEPEDERASVKSISSGHSERSVAANTRHLLHQGYALLHTQL 259
            ....|..      ..|....::::..|    ..||.||:.......:::|..|...|     ..:
Mouse   528 EDGELYTWGEGDFGRLGHGDSNSRNIP----TLVKDISNVGEVSCGSSHTIALSKDG-----RTV 583

  Fly   260 FTFGASNNGLLGSGDHIRRANVMRLQKLDSMGVCSIAAGLEHTVARTLDGRLYHWGLNNHSQLGE 324
            ::||..:||.||.||..|......::.|..|.:..:.||.:.::|.|..|::|.||.......|.
Mouse   584 WSFGGGDNGKLGHGDTNRVYKPKVIEALQGMFIRKVCAGSQSSLALTSTGQVYAWGCGACLGCGS 648

  Fly   325 DVSSPMEITITENTAALPIEQNSALEATCGDYHTLLLNASGQIHSL------------QPAPPMR 377
            ..::.:...:.|..||..|     ::.:.||.|.|.|:...::::.            ...|..:
Mouse   649 SEATALRPKLIEELAATRI-----VDISIGDSHCLSLSHDNEVYAWGNNSMGQCGQGNSTGPITK 708

  Fly   378 HLQQSSTYAQTLLQLQLGA----AW---PRQLRLLMCSGGY-------TLQNQRQF--------- 419
            ..:.|......:.|:..|.    ||   ||..:::.....|       |..:.|.|
Mouse   709 PKKVSGLDGIAIQQISAGTSHSLAWTALPRDRQVVAWHRPYCVDLEESTFSHLRSFLERYCDKIN 773

  Fly   420 ----------QRQYHYYLSHLQSQLQLLLKH---------------RQA---------------- 443
                      .|::|.:   |:..|:||..|               |||
Mouse   774 SEIPPLPFPSSREHHNF---LKLCLKLLSNHLALALAGGVATSILGRQAGPLRNLLFRLMDSTVP 835

  Fly   444 -------VQTLEIW---------QRQSALEPLSALGPLLINWERI-------LCLLVATLH---- 481
                   ::||.:.         :|...|..|...||  ..||.:       |.:::.:|.
Mouse   836 DEIQEVVIETLSVGATMLLPPLRERMELLHSLLPQGP--DRWESLSKGQRMQLDIILTSLQDHTH 898

  Fly   482 --SLEGFYRADFVQPADLLFICHYKEYIDLFD-GYTKAYC------------------DVFSVNG 525
              ||.|:....  ..|||..:|  ..|.:|.| .|....|                  ..::...
Mouse   899 VASLLGYSSPS--DAADLSTVC--MGYGNLSDQPYGSQICHPDTHLAEILMKTLLRNLGFYTDQA 959

  Fly   526 FGEAVVAITGLSSPLAELNEESYV----TRLFQQPFSIYQLFVQFMELLVRTQSEYGEHRVAWSE 586
            |||            .|.|.:.|:    :....||..:::|.....:.|:              .
Mouse   960 FGE------------LEKNSDKYLLGTSSSENSQPAHLHELLCSLQKQLL--------------A 998

  Fly   587 FARHSCISQELAVNTKDFWSSNDRNPRIVQFRGRHRRVILTSALVPLKLVTSGISRSSNSF---- 647
            |...:.:::               |...|....:|.:::|..|       |...|||:|..
Mouse   999 FCHINNVTE---------------NSSSVALLHKHLQLLLPHA-------TDIYSRSANLLKESP 1041

  Fly   648 -----------ILF----SDFLCQVSGNSLYSYPLTT--------LWVWTEGDSLRLTTPEKSFL 689
                       :::    ...|||:. |||...|::.        |.:....|.|....|..:.|
Mouse  1042 WNGSVGEKLRDVIYVSAAGSMLCQIV-NSLLLLPVSVARPLLSYLLDLLPPLDCLNRLLPAAALL 1105

  Fly   690 VSTRSQEMR-----------------------KVWLDQLQSSIIASLGKPL-----GSPV-PSYR 725
               ..||::                       .|||..|:.:|...:|:.|     |||| |..:
Mouse  1106 ---EDQELQWPLHGGPEVIDPAGVPLPQPAQSWVWLVDLERTIALLIGRCLGGMLQGSPVSPEEQ 1167

  Fly   726 STGY-----EFS----REHPKFSRVKACGTWRKGVLHGN------CYLEYPDGSVY------CGE 769
            .|.|     .||    .:.|:..:..:|  ..:..|.||      .|...|:.:||      |.:
Mouse  1168 DTAYWMKTPLFSDGVEMDTPQLDKCMSC--LLEVALSGNEEQKPFDYKLRPEVAVYVDLALGCSK 1230

  Fly   770 ------------------------LQHGIIEGFGKMVIPT----TGLYVGNFKGGRFH-GHGVYE 805
                                    ....:::...:.|:..    |.|........|:. |..:.|
Mouse  1231 EPARSLWISMQDYAVSKDWDSATLSNESLLDTVSRFVLAALLKHTNLLSQACGESRYQPGKSLSE 1295

  Fly   806 MH------------CK--------------------DSPESEVYEGNFC---------------- 822
            ::            ||                    ||.:.:..|.:|.
Mouse  1296 VYRCVYKVRSRLLACKNLELIQTRSSSRDRWITDNQDSADVDPQEHSFTRTIDEEAEMEELAERD 1360

  Fly   823 ----------EGLFHGHGVMRNNRYIYVGEYQANARSGYGVIEDLVSGDKYMGMFADNKRSGIGS 877
                      |.....|.||.....::|.......:......|...|.|:      |...||.||
Mouse  1361 REDGHPEPEDEEEEREHEVMTAGNLVFVLHVTEIFQCFLSAREVARSRDR------DRMNSGAGS 1419

  Fly   878 CITNRGD------YFEGSFSGDDLTGSGVAVFE-NDYYYEGELTLLGPN----------GRGEYY 925
            .:  |.|      ..|...|.|...|..|.... |...:...|.:||.:          ..|:..
Mouse  1420 GV--RADDPPPQSQQERRVSTDLPEGQDVYTAACNSVIHRCALLILGVSPVIDELQKRREEGQLQ 1482

  Fly   926 MPSGDACGGSGAMGTGEFDDTCELIGNKMFGQLSGTWDTVRIQAGELVLNRRFPKYPSS------ 984
            .||..|..|:|.|...|     .|.........|.::..::.:: |..|::     |.|
Mouse  1483 QPSVSASEGTGLMTRSE-----SLTAESRLVHASPSYRLIKSRS-ESDLSQ-----PESDEEGYA 1536

  Fly   985 -LGRQVVD----------------------------HNRKWRSLFN---NFESD----------- 1006
             .||:.||                            |.|.|  .:|   :||||
Mouse  1537 LSGRRNVDFDLASSHRKRGPMHSQLESLSDSWTRLKHTRDW--FYNSSYSFESDFDLTKSLGVHT 1599

  Fly  1007 -LANCTASTSSSGGNQSAGTLRKSSKPTLSTAQIWNCIAVYMSKQRAREGTKPGNYFNNILLSLP 1070
             :.|..:..|...||...   .|..:.::||:...:.||  |.:|:.|...:.......:.|...
Mouse  1600 LIENVVSFVSGDVGNAPG---FKEPEESMSTSPQASIIA--MEQQQLRAELRLEALHQILTLLSG 1659

  Fly  1071 LPQKTSSPLAKARTTTSALSKLQTEAASALDFLGFPPRRIQSQEALNS-KPGGLQRADSLISMGH 1134
            :.:|.:..|..:|:::                 ||     ||...|.| :...|.....|.::||
Mouse  1660 MEEKGNVLLTGSRSSS-----------------GF-----QSSTLLTSVRLQFLAGCFGLGTVGH 1702

  Fly  1135 NTSR----------DLDNSSLASFQLDQSLMNSTVNGDESSTFNESFSKLSHNNNN--------- 1180
            ..::          |...::..:.|::..:....:....|:|...:.....|:...
Mouse  1703 TGTKGESGRLHHYQDGIRAAKRNIQVEIQVAVHKIYQQLSATLERALQANKHHIEAQQRLLLVTV 1767

  Fly  1181 -SISKHMNNIDCSITSTTST---------TSAVLDQ----VPSFGMALVLTEQDVTSIRLYLEQA 1231
             ::|.|...:|.|:..:|..         |..:|.|    :|..|::.:.|...|.|.||
Mouse  1768 FALSVHYQPVDVSLAISTGLLNVLSQLCGTDTMLGQPLQLLPKTGVSQLSTALKVASTRL----- 1827

  Fly  1232 FKDRHHPLYVLNERIANCFHYSYGYWKVKPTPILAKQAMREWESISRRIYRFVRKMFPALPEELC 1296
                   |.:|        ..:.|.:..|.:|                      |:..:|.:.||
Mouse  1828 -------LQIL--------AITTGTYADKLSP----------------------KVVQSLLDLLC 1855

  Fly  1297 QMEGSREVISHITLLYPLVLSEGIYSTLFVLYANKYSRKDEMYRQNLNLAEK------LKDQELV 1355
            ..  .:.::|...:|:.....||           :...::|....:...|||      |:.|...
Mouse  1856 SQ--LKNLLSQTGVLFMASFGEG-----------EEGEEEEKKVDSSGEAEKRDFRAALRKQHAA 1907

  Fly  1356 EL-MGHESFLHNVMLDPKFVESVQTLKELQEKF-SPQDMLTVIQRSTQLLTEAYEHAMAANAAQL 1418
            || :|  .||       .|:..|.:.|.:|.|. ||        :.|::|         .|.|..
Mouse  1908 ELHLG--DFL-------VFLRRVVSSKAIQSKMASP--------KWTEVL---------LNIASQ 1946

  Fly  1419 NADNMIPLT--------MLTMLRAAVPHLGAELALLDD 1448
            ...:.|||.        .|.:|.|.:|  ..|..:.||
Mouse  1947 KCSSGIPLVGNLRTRLLALHVLEAVLP--ACESGVEDD 1982

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Als2	NP_649347.1	RCC1	258..304	CDD:278826	13/45 (29%)
		RCC1_2	292..321	CDD:290274	8/28 (29%)
		RCC1	308..361	CDD:278826	13/52 (25%)
		PH-like	616..715	CDD:302622	27/148 (18%)
		VPS9	1373..1479	CDD:280383	21/85 (25%)
Herc1	XP_006511166.1	ATS1	374..731	CDD:227511	75/346 (22%)
		SPRY_HERC1	2039..2196	CDD:293939
		UBA_HERC1	2760..2803	CDD:270584
		WD40	3448..3799	CDD:392136
		WD40 repeat	3456..3499	CDD:293791
		WD40 repeat	3513..3548	CDD:293791
		WD40 repeat	3610..3648	CDD:293791
		WD40 repeat	3653..3689	CDD:293791
		WD40 repeat	3697..3736	CDD:293791
		WD40 repeat	3774..3810	CDD:293791
		ATS1	4022..4370	CDD:227511
		HECTc	4497..4865	CDD:238033
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5184
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900