DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Als2 and Herc2

DIOPT Version :9

Sequence 1:	NP_649347.1	Gene:	Als2 / 40410	FlyBaseID:	FBgn0037116	Length:	1486	Species:	Drosophila melanogaster
Sequence 2:	NP_001347009.1	Gene:	Herc2 / 15204	MGIID:	103234	Length:	4836	Species:	Mus musculus

Alignment Length:	1818	Identity:	336/1818 - (18%)
Similarity:	559/1818 - (30%)	Gaps:	675/1818 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly    11 GGQE---ERESFTIYHEGRELQLHWRGLPPLQRFPASRICSNAGGELLLLTTDHALYSAKLQANR 72
            |.||   ||.:     :|....|    ||.|||| .|.|||.   ::....:|..|.|..|..|.
Mouse   321 GAQETDNERSA-----QGTSAPL----LPLLQRF-QSIICSK---DVPHTESDMHLLSGPLSPNE 372

  Fly    73 -----------EHMDLQLLRTDVVDMDF------------------------------------- 89
                       ..:.:.|.:|.||.|..
Mouse   373 SFLRYLTLPQDNELAIDLRQTAVVVMAHLDRLATPCMPPLCSSPTSHKGSLQEVIGWGLIGWKYY 437

  Fly    90 --------CSG-----------SQELFVVLT-NGSVQRQATGSGRDVGHPHAWQTL---GFDPLE 131
                    |.|           :::.|::|: ||.|..||..|  |:..|...|.|   ....:.
Mouse   438 ANVIGPIQCEGLASLGVMQVACAEKRFLILSRNGRVYTQAYNS--DMLAPQLVQGLASRNIVKIA 500

  Fly   132 LHAEGVRIRRVCCSAQGVVFVGASGETYVMGSCGEVFKAE-------QQPRHMRLY---EEGKEL 186
            .|::|          ...:.:.|:||.|..| ||:..:..       ::|:.:..:   :.||.:
Mouse   501 AHSDG----------HHYLALAATGEVYSWG-CGDGGRLGHGDTVPLEEPKVISAFSGKQAGKHV 554

  Fly   187 LDLAAGNEHFVMLVAPYNL----------------ADDALQLSVASAKEEPEDERASVKSISSGH 235
            :.:|.|:.:...:.|...|                .|:|:.:.||..|      ...|..::.|.
Mouse   555 VHIACGSTYSAAITAEGELYTWGRGNYGRLGHGSSEDEAIPMLVAGLK------GLKVIDVACGS 613

  Fly   236 SERSVAANTRHLLHQGYALLHTQLFTFGASNNGLLGSGDHIRRANVMRLQKLDSMGVCSIAAGLE 300
            .:....|.|.          :.|::::|..:.|.||.|..........::||..:.|..:..|.:
Mouse   614 GDAQTLAVTE----------NGQVWSWGDGDYGKLGRGGSDGCKTPKLIEKLQDLDVIKVRCGSQ 668

  Fly   301 HTVARTLDGRLYHWGLNNHSQLGEDVSSPMEITITENTAALP-----IEQNSALEATCGDYHTLL 360
            .::|.|.||::|.||..::.:||..         ||.....|     ::....::...|..|.|.
Mouse   669 FSIALTKDGQVYSWGKGDNQRLGHG---------TEEHVRYPKLLEGLQGKKVIDVAAGSTHCLA 724

  Fly   361 LNASGQIHS---------------LQPAP------PMRHL-------QQSSTYAQTLLQLQLGAA 397
            |....::||               .:|.|      ..:|:       .||..::..       :.
Mouse   725 LTEDSEVHSWGSNDQCQHFDTLRVTKPEPTALPGLDSKHIVGIACGPAQSFAWSSC-------SE 782

  Fly   398 WPRQLRLL----MCSGGYTLQNQRQFQRQYHYYLSHLQSQLQLLLKHRQAVQTLE---IWQRQSA 455
            |...||:.    :||..:                    .||.|||  ||..:.::   .|.....
Mouse   783 WSIGLRVPFVVDICSMTF--------------------EQLDLLL--RQVSEGMDGTADWPPPQE 825

  Fly   456 LEPLSALGPLLINWERILCLLVATLHSLEGFYRADFVQPADLLFICHYKEYIDLFDGYTKAYCDV 520
            .|                |:.||||:.|.....|......|       .|::.|..|..
Mouse   826 KE----------------CMAVATLNLLRLQLHAAISHQVD-------PEFLGLGLGSV------ 861

  Fly   521 FSVNGFGEAVVAITGLSSPLAELNEESYVTRLFQQPFSIYQLFVQFMELLVRTQSEYGEHRVAWS 585
             .:|...:.||.:...:..|:.:  :|....:.|..:|:          |:.|..|
Mouse   862 -LLNSLKQTVVTLASSAGVLSTV--QSAAQAVLQSGWSV----------LLPTAEE--------- 904

  Fly   586 EFARHSCISQELAVNTKDFWSSNDRNPRIVQFRGRHRRVILTSALVPLKLVTSGISRSSNSFILF 650
                                                 |....|||:|
Mouse   905 -------------------------------------RARALSALLP------------------ 914

  Fly   651 SDFLCQVSGNSLYSYPLTTLWVWTEGDSLRLTTPEKSFLVSTRSQEMRKVWLDQLQSSIIA--SL 713
                |.||||.:.                  .:|.:.|::            |.|..|::|  .|
Mouse   915 ----CTVSGNEVN------------------ISPGRRFMI------------DLLVGSLMADGGL 945

  Fly   714 GKPLGSPVPSYRSTGYEFSREHPKFSRV----KACGTWRKGVLHGNCYLEYP-DGSVYCGELQHG 773
            ...|.:.:.: .....|..:|..|...:    .:..|:.:.        ..| |..:    :..|
Mouse   946 ESALNAAITA-EIQDIEAKKEAQKEKEIDEQEASASTFHRS--------RTPLDKDL----INTG 997

  Fly   774 IIEGFGKMVIPTTGLY------VGNFKGGRFHGHGVYEMHCKDSPESEVYEGNFCEGLFHGHGVM 832
            |.|..||..:|...|.      :.:....|..........|.|      :|...||.......::
Mouse   998 IYESSGKQCLPLVQLIQQLLRNIASQTVARLKDVARRISSCLD------FEQQSCERSASLDLLL 1056

  Fly   833 RNNRYIYVGEYQANARSGYGVIEDLVSGDKYMGMFADN---KRSGIGSCI--------TNRGD-- 884
            |..|.:               |..|..|:| :|..:|.   :..|:||.:        |:.||
Mouse  1057 RFQRLL---------------ISKLYPGEK-IGPISDTSSPELMGVGSLLKKYTALVCTHIGDIL 1105

  Fly   885 -------------------YFEGSFSG---DDLTGSGVAVFEND---YYYEGELTLLGPNGRGEY 924
                               ..||.|:|   .:|..|.|.:...:   ....|.:.|||  |..|:
Mouse  1106 PVAASIASSSWQHFAEVACVMEGDFTGVLLPELVVSIVLLLSKNASLMQEAGAIPLLG--GLLEH 1168

  Fly   925 YMPSGDACGGSGAMGTGEFDDTCEL----IGNKMFGQLSGTWDTVRIQAGELVLNRRFPKYPSSL 985
            .........|.      |.||..||    |....|     |....| ...|:.|.|:
Mouse  1169 LDRFNHLAPGK------ERDDHEELAWPGIMESFF-----TGQNCR-NNEEVTLIRK-------- 1213

  Fly   986 GRQVVDHNRK---WRSL------FNNFESDLANCTASTSSSGGN----QSAGTLRKSSKPTLSTA 1037
             ..:.:||:.   |..:      ..:|:         |.|..||    |.||     ..|.::.
Mouse  1214 -ADLENHNKDGGFWTVIDGKVYGIKDFQ---------TQSLTGNSILAQFAG-----EDPVVAL- 1262

  Fly  1038 QIWNCIAVYMSKQRAREGTKPGNYF--NNILLSLPLPQKTSSPL--------------AKARTTT 1086
               .....:...|.:......|.|.  :..::::|.....||||              |.....:
Mouse  1263 ---EAALQFEDTQESMHAFCVGQYLEPDQEVVTIPDLGSLSSPLIDTERNLGLLLGLHASYLAMS 1324

  Fly  1087 SALSKLQTEAASALDFLGFPPRRIQSQEALNSKPGGLQRADSLISMGHNTSRDLDN-SSLASFQL 1150
            :.||.::.|.|..|            |.::.|  ||||  .|.|...:|..:|.|: ||.....:
Mouse  1325 TPLSPVEVECAKWL------------QSSIFS--GGLQ--TSQIHYSYNEEKDEDHCSSPGGTPI 1373

  Fly  1151 DQSLMNS--TVNGDESSTFNESFSKLSHNNNNSISKHMNNIDCSI----------TSTTSTTSAV 1203
            .:|.:.|  ...||.|..|.::.:     :||....::.:..|.|          |..|......
Mouse  1374 SKSRLCSHRWALGDHSQAFLQAIA-----DNNIQDYNVKDFLCQIERYCRQCHLTTPITFPPEHP 1433

  Fly  1204 LDQVPSFGMALVLTEQDVTSIRLYL--------EQAFKDRHHPLYVLNE-RI---ANC----FHY 1252
            :::|....:..:|..:|:..:.|.|        ||. |.|..|..|::. |:   |.|    .|.
Mouse  1434 VEEVGRLLLCCLLKHEDLGHVALSLVHVGTLGIEQV-KHRTLPKSVVDVCRVVYQAKCSLIKTHQ 1497

  Fly  1253 SYG-----------------YWKVKPT-----PILAK----QAMREWESISRRIYRFVR-KMFPA 1290
            ..|                 :.:::|.     .|::|    .::..|..|:::|.|..| |..|.
Mouse  1498 EQGRSYKEVCAPVIERLRFLFNELRPAVCSDLSIMSKFKLLGSLPRWRRIAQKIIRERRKKRVPK 1562

  Fly  1291 LPEELCQME--GSRE-------VISH---------ITLLYPLVLSEGIYSTLFVLYANKYSRKD- 1336
            .||.:...|  |:.|       |:.|         |::..|....:.:.:|:..::..|:.:::
Mouse  1563 KPESIDSEEKIGNEESDLEEACVLPHSPINVDKRPISMKSPKDKWQPLLNTVTGVHKYKWLKQNV 1627

  Fly  1337 -EMYRQNLNL--------------AEKLKDQELVELMGHESFLHNVMLDPKFVESVQTLKELQ-- 1384
             .:|.|:..|              .||::...|.:|...|..|..:....|.......|..:|
Mouse  1628 QGLYPQSALLNTIVEFALKEEPVDVEKMRKCLLKQLERAEVRLEGIDTILKLAAKSFLLPSVQYA 1692

  Fly  1385 -----EKFSPQ-----DMLTVIQRSTQLLTEAYEHAMAANAAQLNA---DNM------------- 1423
                 ::..|:     :.||...|...|:.......:.....:|.|   .|:
Mouse  1693 MFCGWQRLIPEGIDIGEPLTDCLRDVDLIPPFNRMLLEVTFGKLYAWAVQNIRSVLMDASARFKE 1757

  Fly  1424 -----IPLTMLTMLRAAVPHLGA------ELALLDDLT---GGPNFQAEMN-GMAGYCYTTLK 1471
                 :||..:|....|.|.||.      .|.:|..||   |..|....:| |......|.|:
Mouse  1758 LGIQPVPLQTITNENPAGPSLGTIPQARFLLVMLSMLTLQHGANNLDLLLNSGTLALTQTALR 1820

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Als2	NP_649347.1	RCC1	258..304	CDD:278826	10/45 (22%)
		RCC1_2	292..321	CDD:290274	9/28 (32%)
		RCC1	308..361	CDD:278826	13/57 (23%)
		PH-like	616..715	CDD:302622	17/100 (17%)
		VPS9	1373..1479	CDD:280383	27/142 (19%)
Herc2	NP_001347009.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	58..90
		RCC1 1-1	416..462		2/45 (4%)
		RCC1 1-2	463..513		14/61 (23%)
		ATS1	<494..787	CDD:227511	59/335 (18%)
		RCC1 1-3	514..569		11/55 (20%)
		RCC1 1-4	570..621		8/56 (14%)
		RCC1 1-5	624..675		11/50 (22%)
		RCC1 1-6	676..727		14/59 (24%)
		RCC1 1-7	729..779		7/49 (14%)
		Cyt-b5	1212..1283	CDD:365921	15/97 (15%)
		MIB_HERC2	1871..1930	CDD:369043
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2351..2376
		UBA_HERC2	2461..2508	CDD:270585
		Cul7	2555..2632	CDD:371573
		SH3_15	2640..2697	CDD:375773
		ZZ_HERC2	2707..2751	CDD:239084
		APC10-HERC2	2766..2911	CDD:176485
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	2928..2947
		ATS1	2957..3323	CDD:227511
		RCC1 2-1	2959..3010
		RCC1 2-2	3011..3065
		RCC1 2-3	3066..3117
		RCC1 2-4	3119..3169
		RCC1 2-5	3172..3223
		RCC1 2-6	3225..3275
		RCC1 2-7	3276..3327
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3479..3499
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3517..3537
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	3604..3632
		RCC1 3-1	3953..4004
		RCC1 3-2	4006..4058
		ATS1	4025..4330	CDD:227511
		RCC1 3-3	4060..4110
		RCC1 3-4	4112..4164
		RCC1 3-5	4166..4216
		RCC1 3-6	4218..4268
		RCC1 3-7	4270..4320
		HECTc	4423..4794	CDD:238033
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	4806..4836
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5184
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	0	0.000	Not matched by this tool.
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	1	0.900