DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Als2 and als2b

DIOPT Version :9

Sequence 1:	NP_649347.1	Gene:	Als2 / 40410	FlyBaseID:	FBgn0037116	Length:	1486	Species:	Drosophila melanogaster
Sequence 2:	XP_009300450.1	Gene:	als2b / 100318899	ZFINID:	ZDB-GENE-080929-1	Length:	1706	Species:	Danio rerio

Alignment Length:	1547	Identity:	382/1547 - (24%)
Similarity:	604/1547 - (39%)	Gaps:	437/1547 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   120 HAWQTLGFDPLELHAEGVRIRRVCCSAQGVVFVGASGETYVMGSC----GE----VFKA------ 170
            |:.|..| ||..|.|....|.    .||.|..:|::....|: ||    ||    .::|
Zfish   402 HSAQLSG-DPANLFASEPLIP----VAQSVTPMGSALNNLVV-SCASAVGERVVSTYEALSLRKV 460

  Fly   171 ----------EQQPRHMRLYE--EGKELLDLAAGNEHFV----MLVAPYNLADDALQLSVASAKE 219
                      |:....:||.|  :||:...|....|...    .|..|..|:.|..:..|.::..
Zfish   461 IGYWVPGEGRERVEERLRLEESMQGKKSSSLGDIREEEAELSRRLSLPGLLSQDNAESEVKASSS 525

  Fly   220 EPEDE------------RASVKSISSGHSERSVAANTRHLLHQGYALLHTQLFTFGASNNGLLGS 272
            ....|            |.....::||    .:.....||     ..|.|:::::|...:|.||.
Zfish   526 NTTTEVSPRLLRRTSRPRMRAVPLASG----GIPETDAHL-----PSLQTEVWSWGQGQDGQLGH 581

  Fly   273 GDHIRRANVMRLQKLDSMGVCSIAAGLEHTVARTLDGRLYHWGLNNHSQLGEDVSSPMEITITEN 337
            ||.:.|.....::.|:...|..:|||..|::|.|...:::.||.|...|||...|......:|:.
Zfish   582 GDLLPRLQPDCVKSLNGKEVLKVAAGAHHSLALTAQSQVFSWGCNTSGQLGHMESPTTFPQLTKL 646

  Fly   338 TAALPIEQNSALEATCGDYHTLLLNASGQIHSLQP------------APPMRHLQQSSTYAQ--T 388
            :..:.:....|     |..||||| |.|.  .:||            ..|....:|::.|.|  .
Zfish   647 SDGIRVWDIGA-----GLQHTLLL-ADGD--CIQPILYYSGQQVKEVPEPTEEEEQTAEYTQQPV 703

  Fly   389 LLQLQLGAAWPRQLRLLMCSGGYT---------------LQNQRQFQRQYHYYLSHLQS-QLQLL 437
            ||...:...:...    :.:||.|               |......:|:|:..||:::| .||.|
Zfish   704 LLPFCMNLGYVSS----VYAGGRTCAALTDCNVMGFIASLHELAAAERKYYCKLSNIKSLLLQPL 764

  Fly   438 LKHRQAVQTLEIWQRQSALEPLSA--LGPLLINWERILCLLVATLHSLEGF-YRADFVQPADLLF 499
            ||    :.:|     .|||...||  |..|:....|:..|......||..| .|:..::...:|.
Zfish   765 LK----LDSL-----SSALGQSSAGLLQNLIGRMSRLSQLTGQNSASLTSFLQRSRDIRGLVILE 820

  Fly   500 ICHYKEYIDLFDGYTKAYCDVFSVNGFGEAVVA----ITGLSSP----LAELNEE-----SYVTR 551
            ..|.  ::|.:..|:.|..|:..:.|| :|:|.    :.|..:.    |:|.:||     ..:..
Zfish   821 DAHL--FLDTYSEYSSAVGDLLVMGGF-QALVKPCHDVFGKGAEVVQRLSECSEEGLTLADVLAH 882

  Fly   552 LFQQPFSIYQLFVQFMELLVR-------TQSEYGEHRVAWSEF---ARHSCISQELAVNTKDFWS 606
            ||..|.....   ::..||::       :..:|.:.:.|.|:|   |.|....::.|..|..||.
Zfish   883 LFYLPIKHLH---EYGRLLLKLATCYEVSSVDYQKLQDACSKFESMALHLKRRRKEAEYTLHFWK 944

  Fly   607 SNDRNPRIVQFRGR--------HRRVILTSALVPLKLVTSGISRSSNSFILFSDFLCQVSG---- 659
            |         |.|:        .||:|..|:...|.|..:| ..|.|.||||:|.|....|
Zfish   945 S---------FPGKMTDSLRKPSRRLICESSNKALTLQNAG-RFSVNWFILFNDALVHAQGMVPY 999

  Fly   660 NSLYS----YPLTTLWVWTEGD------SLRLTTPEKSFLVSTRSQEMRKVWLDQLQSSI---IA 711
            .||:|    :||.||||....:      .|::|:||:||.:...|...:..||..:..::   ::
Zfish  1000 KSLFSTHHIFPLATLWVEPISEESTGVYGLKVTSPEESFALLASSPAEKAKWLRSINQAVEQALS 1064

  Fly   712 SLGKPLGSP----------VPSYRSTGYEFSREHPKFSRVKAC---GTWRKGVLHGNCYLEYPDG 763
            .||.. |.|          .|..|:..|.|.::    ||:|..   |.|..|..||...:::|||
Zfish  1065 GLGHD-GIPPSTGMTQRADPPISRTASYTFYKD----SRLKDAKYDGRWVSGKPHGRGVVKWPDG 1124

  Fly   764 SVYCGELQHGIIEGFGKMVIPTTGL-----YVGNFKGGRFHGHGVYEMHCKDSPESEVYEGNFCE 823
            .:|.|..::|..:|||..|:....|     |.|.:|.|:.||.|.:..     ...|||||:|.:
Zfish  1125 RMYTGTFKNGFEDGFGDYVVSNKTLNSCDHYQGQWKDGKMHGFGTFRY-----ASGEVYEGSFQD 1184

  Fly   824 GLFHGHGVMRNNRY------IYVGEYQANARSGYGVIEDLVSGDKYMGMFADNKRSGIGSCITNR 882
            .:.||||::|:.:.      :::|::|.:.:|||||.:|:..|:|||||:.|::|.|.|..:|..
Zfish  1185 NMRHGHGMLRSGKLNSTSPSVFIGQWQYDKKSGYGVFDDITRGEKYMGMWLDDQRQGNGVVVTQF 1249

  Fly   883 GDYFEGSFSGDDLTGSGVAVFENDYYYEGEL----TLLGPNGRGEYYMPSGDACGGS--GAMGTG 941
            |.|:||:||.:.:.|:||.:.|:|..:|||.    ||   ||:|...||:||...||  |..||
Zfish  1250 GLYYEGAFSNNKMMGTGVLLSEDDTTFEGEFLEDWTL---NGKGVLTMPNGDYIEGSFCGVWGT- 1310

  Fly   942 EFDDTCELIGNKMFGQL--SGTWDTVRIQAGELVLNRRFPKYPSSLGRQVVDHNRKWRSLFNNFE 1004
                     |.||.|..  ...:|:.:.:...|           .|||..|....||:::|....
Zfish  1311 ---------GLKMSGSYYKPSLYDSDKEKGHAL-----------KLGRLAVHSEEKWKAVFLECW 1355

  Fly  1005 SDLANCTASTSSSGGNQSAGTLRKSSKPTLSTAQIWNCIAVYMSKQRAREGTKPGNYFNNILLSL 1069
            :.| .|.:....                  .|...|:.||                    |.|:.
Zfish  1356 NRL-GCDSPVQG------------------QTTTAWDNIA--------------------IALTA 1381

  Fly  1070 PLPQKTSSPLAKARTTTSALSKLQTEAASALDFLGFPPRRIQSQEALNSKPGGLQRADSLISMGH 1134
            .|.|:..||                                                 .|:|..|
Zfish  1382 NLRQQRDSP-------------------------------------------------ELLSRSH 1397

  Fly  1135 NTSRDLDNSSLASFQLDQSLMNSTVNGDESSTFNESFSKLSHNNNNSISKHMNNIDCSITSTTST 1199
                   :.:|.|.:                               .|.:|:..:...:..|
Zfish  1398 -------HKTLESLE-------------------------------CIPQHVGPVTLEVYHT--- 1421

  Fly  1200 TSAVLDQVPSFGMALVLTEQDVTSIRLYLEQAFKDRHHPLYVLNERIANCFHYSY-GYWKVKPTP 1263
                                    ||.||.:|.....|||..|.|.:.:.:..:| |   |....
Zfish  1422 ------------------------IRRYLVKACDTPLHPLGRLVETLVSVYRMTYVG---VGANR 1459

  Fly  1264 ILAKQAMREWESISRRIYRFVRKMFPALPEE-------------------LCQMEGSREVISHIT 1309
            .|..||:.|..|...||::.||.:||.||||                   |.:......|:|...
Zfish  1460 RLLPQAVNEITSYLSRIFQLVRFLFPELPEEGGSLADPPKEKVGTDADGKLLESPKPGCVVSSSA 1524

  Fly  1310 LLYPLVLSEGIYSTLFVLYANKYSRKDEMYRQNLNLAEKLKDQELVELMGHESFLHNVML----- 1369
            ||.|::|.. :|..||.|||.:..::|::|.:.:....|..|..|:..:|.:.....|.:
Zfish  1525 LLLPVLLPR-LYPPLFTLYALEKEKEDDVYWECVLRLNKQPDLALLAFLGVQQKFWPVTVTIHGE 1588

  Fly  1370 ---------DPKFVESVQTLKELQEKFSPQDMLTVIQRSTQLLTEAYEHAMAANAAQLNADNMIP 1425
                     |..|..:|:||:::...|:|.|.|.|||.:.:.:|:.. .|:.......:.|::.|
Zfish  1589 KQQVLSSTKDACFASAVETLQQISTTFTPSDKLQVIQLTFEEITQEV-LALLKQDFLWSMDDLFP 1652

  Fly  1426 LTMLTMLRAAVPHLGAELALLDDLTGGPNFQAEMNGMAGYCYTTLKAAYEHV 1477
            :.:..:|||.:.:||:|::|::||. .|..|   :|..|..:|||||.|..:
Zfish  1653 VFLFVVLRARIRNLGSEVSLIEDLM-DPCVQ---HGEHGIMFTTLKACYYQI 1700

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Als2	NP_649347.1	RCC1	258..304	CDD:278826	13/45 (29%)
		RCC1_2	292..321	CDD:290274	10/28 (36%)
		RCC1	308..361	CDD:278826	13/52 (25%)
		PH-like	616..715	CDD:302622	37/123 (30%)
		VPS9	1373..1479	CDD:280383	34/105 (32%)
als2b	XP_009300450.1	RCC1_2	88..117	CDD:290274
		RCC1	104..160	CDD:278826
		RCC1	165..211	CDD:278826
		RCC1	567..614	CDD:278826	13/46 (28%)
		RCC1	619..665	CDD:278826	13/50 (26%)
		PH_alsin	952..1065	CDD:241423	34/113 (30%)
		COG4642	1085..1290	CDD:226989	77/216 (36%)
		MORN	1104..1124	CDD:280628	6/19 (32%)
		MORN	1153..1173	CDD:197832	7/24 (29%)
		VPS9	1603..1702	CDD:280383	33/103 (32%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	58	1.000	Domainoid score	I10743
eggNOG	1	0.900	-	-		E1_COG4642
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	319	1.000	Inparanoid score	I2507
OMA	1	1.010	-	-		QHG47592
OrthoDB	1	1.010	-	-		D25604at33208
OrthoFinder	1	1.000	-	-		FOG0002992
OrthoInspector	1	1.000	-	-		otm24678
orthoMCL	1	0.900	-	-		OOG6_106878
Panther	1	1.100	-	-	LDO	PTHR46089
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R5555
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	14	13.870