Protein Alignment Ac78C and Adcy8

DIOPT Version :10

• Sequence 1: NP_001262138.1
• Gene: Ac78C / 40333
• FlyBaseID: FBgn0024150
• Length: 1727
• Species: Drosophila melanogaster
• Sequence 2: NP_033753.2
• Gene: Adcy8 / 11514
• MGIID: 1341110
• Length: 1249
• Species: Mus musculus
• Alignment Length: 1528
• Identity: 491/1528 - (32%)
• Similarity: 735/1528 - (48%)
• Gaps: 394/1528 - (25%)

  Fly   189 DNGSGDLPPSLRASTTSILSNLRKKQ-----QGGPLRGGVAKQT-------------PLRVQSVR 235
            |:|||..|..|...|.  :.::.:::     :||. .|||:::.             .|...||.
Mouse    26 DDGSGSRPQRLLWQTA--VRHITEQRFIHGHRGGG-GGGVSRKASNPAGSGPNHHAPQLSSDSVL 87

  Fly   236 IVEAKRAYGPKRRTESCSIFGNSNFEHDLESGNSLASIPGQSQ-------RPLNNEMYSAFKSGN 293
            .:   .:.||..|..:..  |...|.....||::..|..|...       .|.|::.:  ...|.
Mouse    88 PL---YSLGPGERAHNTG--GTKVFPERSGSGSASGSGGGGDLGFLHLDCAPSNSDFF--LNGGY 145

  Fly   294 VVKGILCPSLTNSFKQSSLERSYLTYTHRQRQKSLIIVNVVDFVLKIVLAFVWI-MRRSELGPIE 357
            ..:|::.|:|.||||...|||.|..|...||:||.:::||:|.:.|:.|..:.: :..:.:.|::
Mouse   146 SYRGVIFPTLRNSFKSRDLERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLK 210

  Fly   358 SDDGTSMATAITWSVCCGIANMAICFLGYWR--CFANNYLHWAAVCTWVLFNIQ--------GFV 412
                     .|......|| .:.||.|...|  ..::.||.::.|.|||....|        |.:
Mouse   211 ---------GILLGFFTGI-EVVICALVVVRKDNTSHTYLQYSGVVTWVAMTTQILAAGLGYGLL 265

  Fly   413 GQGVGFADREYLVWYILFVIFVPYAMLPLPLKWCVVGGTITASCHLAVITIIKLQHGEATINPEC 477
            |.|:|         |:||.:|..|:||||||.|.::.|..|:...:.:..:|          |..
Mouse   266 GDGIG---------YVLFTLFATYSMLPLPLTWAILAGLGTSLLQVTLQVLI----------PRL 311

  Fly   478 VLF---QIFANFILYTAINVAGMYTKYLTDRGQRLAFIETHKAMEHKKESEKELQRTQKLLDSIL 539
            .:|   |:.|..:|:..:|.||::..||:||.||.||:||.:.:|.:...|.|.||.::|:.|:|
Mouse   312 AVFSINQVLAQVVLFMCMNTAGIFISYLSDRAQRQAFLETRRCVEARLRLETENQRQERLVLSVL 376

  Fly   540 PNIVNNQIRSEMYKGTDPTVETQFNKLYVYPMDNVSILFADIKGFTELASKTSAQQLVKILNDLF 604
            |..|..::.::|....|..::.||:::|::..:||||||||:||||.|::..|||:||::||:||
Mouse   377 PRFVVLEMINDMTNVEDEHLQHQFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELF 441

  Fly   605 ARFDRIAEDNHCLRVKLLGDCYYCVSQFESDNWKTRPDHAVCSVETGLHMIKAIKDVRLHTHVDL 669
            |||||:|.::||||:|:|||||||||...    :.|.|||.|.||.||.|||.|:.||..|..|:
Mouse   442 ARFDRLAHEHHCLRIKILGDCYYCVSGLP----EPRRDHAHCCVEMGLSMIKTIRFVRSRTKHDV 502

  Fly   670 NMRIGIHSGSVMCGVLGNKKWHFDVWSNDVIIANHMESGGIPGRVHISEATLKCLNDAYEVEPGN 734
            :||||||||||:|||||.:||.|||||.||.|||.:||||||||:|||:|||.|||..|.||.|:
Mouse   503 DMRIGIHSGSVLCGVLGLRKWQFDVWSWDVDIANKLESGGIPGRIHISKATLDCLNGDYNVEEGH 567

  Fly   735 GGCRDNHLKMLNVKTYLIKRTEPLRPKRRFGTRSSAHLAGSVATAAPPCATPTALPKSISASGSG 799
            |..|:..|:..|::|||||:.|.
Mouse   568 GKERNEFLRKHNIETYLIKQPEE------------------------------------------ 590

  Fly   800 SIGGVTATGNDGASIDGRSLEYAATIAVPAQQPTQLLQQQQKKNISLNSLPN--VVEGVAMDSRR 862
                                                         ||..||.  |.|.|:...||
Mouse   591 ---------------------------------------------SLLCLPEDIVKESVSCSDRR 610

  Fly   863 IRNGCGVMGGAGGTGGGGTGPCGMSTVSSPTAMMSAPLEVQLRPRNGACSIQNLAEAIDGKGLII 927
                   ..||..|.|
Mouse   611 -------NSGATFTEG------------------------------------------------- 619

  Fly   928 EDESTTDWIPEIPFKNLNSPED---GLNRADSILVDTKEDHRVSVAVLDEEIDEFIEQNIQINSN 989
                  .|.||:||.|:...::   .|.|....|:.......:.|....|||::.||..|.:.|.
Mouse   620 ------SWSPELPFDNIVGKQNTLAALTRNSINLLPNHLAQALHVQSGPEEINKRIEHTIDLRSG 678

  Fly   990 KEIRREYLNPWTLKFKDKSQEQKFCQLREDMFRSNMLCVFVIWIFMVLCQVII-IPRCTRLIICL 1053
            .::|||::.|::|.|||.|.|.|:.|:|:::|:||::|.|::.:|:...|.:: ..|...:.|..
Mouse   679 DKLRREHIKPFSLMFKDSSLEHKYSQMRDEVFKSNLVCAFIVLLFITAIQSLLPSSRLMPMTIQF 743

  Fly  1054 SVGTVILTFCCVLVMAEEFPGLPRCLKTNSAKLVHQRQRRTLFICGVIVSMCMLSAIGLVLCPSS 1118
            |:..::.:...::..||::..||..|            |:|   |      |.:         :.
Mouse   744 SILIMLHSALVLITTAEDYKCLPLIL------------RKT---C------CWI---------NE 778

  Fly  1119 TYIGTADEHSRFLRMVSPYSNISMTEKEFKLNVYLSATIHHNITISTPASGGPTPLVDITSSGPS 1183
            ||:..                          ||.:.|:|..|
Mouse   779 TYLAR--------------------------NVIIFASILIN----------------------- 794

  Fly  1184 DEFVRSTLNALTLNLTPPLPQSHRPYTLTTNGHSVSLSNLSYSDASVMQSSSDVEGQCAHPEYLV 1248
              |:.:.||.|..:....:|                |.||:::.::|.   :|:   |::|||.|
Mouse   795 --FLGAVLNILWCDFDKSIP----------------LKNLTFNSSAVF---TDI---CSYPEYFV 835

  Fly  1249 FTWVLCLVSLATALKLYYLVKALMALAMVAFYTTL-------IMMKFG----SGDSFSLVELSRL 1302
            ||.||.:|:.|..|:|..::|..:.|.|:|.|..|       :.:.:.    ||:.|
Mouse   836 FTGVLAMVTCAVFLRLNSVLKLAVLLIMIAIYALLTETIYAGLFLSYDNLNHSGEDF-------- 892

  Fly  1303 GMPLGVQ--MLILLISFLVMVCYHARLVEVTSRLDFIWKEQAERELTNMKSNRALNDTLIKNILP 1365
               ||.:  .|:|:..||:.|.||.:.:|.|:||||:|:.||:.|:..||..|..|:.:::||||
Mouse   893 ---LGTKEASLLLMAMFLLAVFYHGQQLEYTARLDFLWRVQAKEEINEMKELREHNENMLRNILP 954

  Fly  1366 DHVATYYL-SDEHTDELYSKMHNLCGVMFASIPNFQDFYSE-DIDN-GKACIRILNEIICDFDEL 1427
            .|||.::| .|...:||||:.::..||||||||.|.||||: :::| |..|:|:|||||.|||||
Mouse   955 SHVARHFLEKDRDNEELYSQSYDAVGVMFASIPGFADFYSQTEMNNQGVECLRLLNEIIADFDEL 1019

  Fly  1428 LEEPRFASVEKIKTVGATYMAAAGLNHEHLRLRGETSEDS---VCDLVEFAFAMKQKLEEINGDA 1489
            |.|.||..:|||||:|:||||.:||:.|.     :..||.   :|.|.:|:.|:.:.::|||..:
Mouse  1020 LGEDRFQDIEKIKTIGSTYMAVSGLSPEK-----QQCEDKWGHLCALADFSLALTESIQEINKHS 1079

  Fly  1490 FNNFQLRVGICSGPLVSGVIGARKPVYDIWGNTVNVASRMDSTGENWRVQVPENTAELLCSRGYT 1554
            ||||:||:||..|.:|:|||||:||.|||||.|||:||||||||.:.|:||||.|..:|..:|:.
Mouse  1080 FNNFELRIGISHGSVVAGVIGAKKPQYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFA 1144

  Fly  1555 CVKRGEIAVKG----KGMMTTFYVHPKGISESQLISPVRMPAGIPLA-------QTPNLQRQTS- 1607
            ...||||.|||    :|.:.|:::..:......::.|.|:|....||       |:.|.|||..
Mouse  1145 FDYRGEIYVKGISEQEGKIKTYFLLGRVQPNPFILPPRRLPGQYSLAAVVLGLVQSLNRQRQKQL 1209

  Fly  1608 -HHGSFSAVVFGMLQASKRSTAIAGTPTGTPSP 1639
             :..|.|.::   .....|.|.:  ||:| |.|
Mouse  1210 LNENSNSGII---KSHYNRRTLL--TPSG-PEP 1236

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Ac78C	NP_001262138.1	AC_N	<216..551	CDD:318454	103/368 (28%)
		Guanylate_cyc	566..753	CDD:425528	115/186 (62%)
		Adcy_cons_dom	929..1021	CDD:461877	30/94 (32%)
		Guanylate_cyc	1381..1576	CDD:425528	106/203 (52%)
Adcy8	NP_033753.2	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000250|UniProtKB:P40146	1..180		42/163 (26%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000250|UniProtKB:P40146	1..107		19/88 (22%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	14..33		4/6 (67%)
		Essential for CALM1 interaction. /evidence=ECO:0000250|UniProtKB:P40146	38..40		0/1 (0%)
		Essential for CALM1 interaction. /evidence=ECO:0000250|UniProtKB:P40146	49..51		0/1 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	50..118		15/73 (21%)
		AcyC	212..565	CDD:441717	171/376 (45%)
		Guanylate_cyc	403..587	CDD:425528	116/187 (62%)
		Adcy_cons_dom	615..710	CDD:461877	32/149 (21%)
		Guanylate_cyc	971..1170	CDD:425528	106/203 (52%)
		Involved in CALM1 interaction. /evidence=ECO:0000250|UniProtKB:P40146	1107..1249		51/136 (38%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000250|UniProtKB:P40146	1198..1213		5/14 (36%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1221..1249		7/19 (37%)