Protein Alignment polo and plk3

DIOPT Version :10

• Sequence 1: NP_524179.2
• Gene: polo / 40232
• FlyBaseID: FBgn0003124
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: NP_958465.2
• Gene: plk3 / 334202
• ZFINID: ZDB-GENE-030131-6134
• Length: 660
• Species: Danio rerio
• Alignment Length: 633
• Identity: 214/633 - (33%)
• Similarity: 341/633 - (53%)
• Gaps: 81/633 - (12%)

  Fly     3 AKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQK 67
            :|.|....::...:||....::|.:.:..||||||:|||:.|:..:.::|.|::.:..:.|.:|:
Zfish    37 SKSEHVKPELAQVVVDAKTGRSYCKGKLLGKGGFARCYEMTDLANNKMYAVKVIPQSRVSKPHQR 101

  Fly    68 EKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQI 132
            ||...||.:|:||.|.::|||.::|||..||||.||||.::|:..:.|.|.::|:.|.|||:.||
Zfish   102 EKIINEIELHKSLQHKHVVKFSHHFEDQDNIYIFLELCSRKSLAHIWKARHTLTDPEVRYYLRQI 166

  Fly   133 IQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTK 197
            |..:||||:..|:||||||||.|:|:.:.:::||||||.::|...:||||:||||||:|||:|.:
Zfish   167 ISSLKYLHNKGILHRDLKLGNFFVNENMELRLGDFGLAAKLETVEQRKKTICGTPNYLAPEVLNR 231

  Fly   198 KGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNP 262
            :||..|.|:||:||||||||.|.|||||..||:||..||:.:|.:||.|...|..::.::||.||
Zfish   232 QGHGTESDVWSLGCVMYTLLCGNPPFETLDLKETYKCIKEVKYSLPSSLTPSAQKLISSILQKNP 296

  Fly   263 ESRPAIGQLLNFE-FLKG---SKVPMFLPSSCLTMAPRIG--------------------SNDTI 303
            ..|..:.|:|..: |.||   .|:|   ||||: |.|.:.                    .:.|:
Zfish   297 CDRLTLDQILAHDYFTKGFTPEKLP---PSSCV-MVPELNPPSPAKKFFTKMAKSLFGKKKSKTV 357

  Fly   304 EDSMHRK--------------------------------PLMEMNGIRPDDTRLESTFLKA---N 333
            |.:...:                                |.:::....|.||..|....|:   :
Zfish   358 EKTTSEEKDDISKLVSGMVKHSIGRQMSYKTMGVNEATSPTVQLASSGPLDTPAEEESRKSASRS 422

  Fly   334 LHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPRILQGNLGDENTDP----AAQPLFWISK 394
            ....:.:|...|.......:..||..:.|.:.::..|        ..:.:|    ..:|..|::|
Zfish   423 FKGTVASSTDGCDDIPTPSAVAESAMKVLNSCLSSMP--------TASRNPPCLVKTKPFIWVTK 479

  Fly   395 WVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKMKL 459
            |||||:||||||||.::.:||:||:.|.|.|...:..||:...:.|..........:.|..:..:
Zfish   480 WVDYSNKYGFGYQLSNQNVGVLFNEGTHLSLCDQRRTVHYCLTNNKHFSFPANALPEKLQSQKHI 544

  Fly   460 LSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLNF-SDHMKLIL 523
            :.....||.::|::.|  ::|.|....|..|.|..|.:|..|:||...|.::|:|| :||.|:||
Zfish   545 VDLMANYMEQNLMEGG--DINCEDQSSSSSPLLLQWIKTDHALVMLFDNRTLQVNFYTDHTKIIL 607

  Fly   524 CPRMSA---ITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLR 568
            |....|   :|::.:|:...:|..:.:.|.|.|.:|.|::.|..:.|:
Zfish   608 CKPSDASYLVTFISKERVSYSYPLNVLSELGCSPELRQRLHYVVQLLQ 655

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
polo	NP_524179.2	STKc_PLK	23..277	CDD:271001	122/254 (48%)
		POLO_box_1	388..475	CDD:240561	30/86 (35%)
		POLO_box_2	490..570	CDD:240560	28/83 (34%)
plk3	NP_958465.2	STKc_PLK3	57..311	CDD:271091	122/253 (48%)
		POLO_box_1	473..561	CDD:240561	31/89 (35%)
		POLO_box_2	573..656	CDD:240560	28/83 (34%)