Protein Alignment polo and plk3

DIOPT Version :9

• Sequence 1: NP_001014592.1
• Gene: polo / 40232
• FlyBaseID: FBgn0003124
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: NP_958465.1
• Gene: plk3 / 334202
• ZFINID: ZDB-GENE-030131-6134
• Length: 644
• Species: Danio rerio
• Alignment Length: 633
• Identity: 214/633 - (33%)
• Similarity: 341/633 - (53%)
• Gaps: 81/633 - (12%)

  Fly     3 AKPEDKSTDIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQK 67
            :|.|....::...:||....::|.:.:..||||||:|||:.|:..:.::|.|::.:..:.|.:|:
Zfish    21 SKSEHVKPELAQVVVDAKTGRSYCKGKLLGKGGFARCYEMTDLANNKMYAVKVIPQSRVSKPHQR 85

  Fly    68 EKTAQEITIHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQI 132
            ||...||.:|:||.|.::|||.::|||..||||.||||.::|:..:.|.|.::|:.|.|||:.||
Zfish    86 EKIINEIELHKSLQHKHVVKFSHHFEDQDNIYIFLELCSRKSLAHIWKARHTLTDPEVRYYLRQI 150

  Fly   133 IQGVKYLHDNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTK 197
            |..:||||:..|:||||||||.|:|:.:.:::||||||.::|...:||||:||||||:|||:|.:
Zfish   151 ISSLKYLHNKGILHRDLKLGNFFVNENMELRLGDFGLAAKLETVEQRKKTICGTPNYLAPEVLNR 215

  Fly   198 KGHSFEVDIWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNP 262
            :||..|.|:||:||||||||.|.|||||..||:||..||:.:|.:||.|...|..::.::||.||
Zfish   216 QGHGTESDVWSLGCVMYTLLCGNPPFETLDLKETYKCIKEVKYSLPSSLTPSAQKLISSILQKNP 280

  Fly   263 ESRPAIGQLLNFE-FLKG---SKVPMFLPSSCLTMAPRIG--------------------SNDTI 303
            ..|..:.|:|..: |.||   .|:|   ||||: |.|.:.                    .:.|:
Zfish   281 CDRLTLDQILAHDYFTKGFTPEKLP---PSSCV-MVPELNPPSPAKKFFTKMAKSLFGKKKSKTV 341

  Fly   304 EDSMHRK--------------------------------PLMEMNGIRPDDTRLESTFLKA---N 333
            |.:...:                                |.:::....|.||..|....|:   :
Zfish   342 EKTTSEEKDDISKLVSGMVKHSIGRQMSYKTMGVNEATSPTVQLASSGPLDTPAEEESRKSASRS 406

  Fly   334 LHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPRILQGNLGDENTDP----AAQPLFWISK 394
            ....:.:|...|.......:..||..:.|.:.::..|        ..:.:|    ..:|..|::|
Zfish   407 FKGTVASSTDGCDDIPTPSAVAESAMKVLNSCLSSMP--------TASRNPPCLVKTKPFIWVTK 463

  Fly   395 WVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKMKL 459
            |||||:||||||||.::.:||:||:.|.|.|...:..||:...:.|..........:.|..:..:
Zfish   464 WVDYSNKYGFGYQLSNQNVGVLFNEGTHLSLCDQRRTVHYCLTNNKHFSFPANALPEKLQSQKHI 528

  Fly   460 LSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLNF-SDHMKLIL 523
            :.....||.::|::.|  ::|.|....|..|.|..|.:|..|:||...|.::|:|| :||.|:||
Zfish   529 VDLMANYMEQNLMEGG--DINREDQSSSSSPLLLQWIKTDHALVMLFDNRTLQVNFYTDHTKIIL 591

  Fly   524 CPRMSA---ITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLR 568
            |....|   :|::.:|:...:|..:.:.|.|.|.:|.|::.|..:.|:
Zfish   592 CKPSDASYLVTFISKERVSYSYPLNVLSELGCSPELRQRLHYVVQLLQ 639

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
polo	NP_001014592.1	STKc_PLK	23..277	CDD:271001	122/254 (48%)
		POLO_box_1	388..475	CDD:240561	30/86 (35%)
		POLO_box_2	490..570	CDD:240560	28/83 (34%)
plk3	NP_958465.1	STKc_PLK3	41..295	CDD:271091	122/253 (48%)
		S_TKc	43..295	CDD:214567	122/251 (49%)
		POLO_box_1	457..545	CDD:240561	31/89 (35%)
		POLO_box_2	557..640	CDD:240560	28/83 (34%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_KOG0575
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D373288at33208
OrthoFinder	1	1.000	-	-		FOG0000754
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100759
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R930
SonicParanoid	1	1.000	-	-		X638
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	8	7.750