Protein Alignment polo and Plk1

DIOPT Version :10

• Sequence 1: NP_524179.2
• Gene: polo / 40232
• FlyBaseID: FBgn0003124
• Length: 576
• Species: Drosophila melanogaster
• Sequence 2: NP_035251.3
• Gene: Plk1 / 18817
• MGIID: 97621
• Length: 603
• Species: Mus musculus
• Alignment Length: 570
• Identity: 305/570 - (53%)
• Similarity: 415/570 - (72%)
• Gaps: 26/570 - (4%)

  Fly    11 DIPDRLVDINQRKTYKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQKEKTAQEIT 75
            :||:.|||...|:.|.|.||.||||||||:||.|.:|.:|||||||.|.|::|.:||||.:.||:
Mouse    39 EIPEVLVDPRSRRQYVRGRFLGKGGFAKCFEISDADTKEVFAGKIVPKSLLLKPHQKEKMSMEIS 103

  Fly    76 IHRSLNHPNIVKFHNYFEDSQNIYIVLELCKKRSMMELHKRRKSITEFECRYYIYQIIQGVKYLH 140
            |||||.|.::|.||::||||..:::|||||::||::|||||||::||.|.|||:.||:.|.:|||
Mouse   104 IHRSLAHQHVVGFHDFFEDSDFVFVVLELCRRRSLLELHKRRKALTEPEARYYLRQIVLGCQYLH 168

  Fly   141 DNRIIHRDLKLGNLFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVD 205
            .|::|||||||||||||:.|.||||||||||::||||||||||||||||||||:|:|||||||||
Mouse   169 RNQVIHRDLKLGNLFLNEDLEVKIGDFGLATKVEYEGERKKTLCGTPNYIAPEVLSKKGHSFEVD 233

  Fly   206 IWSIGCVMYTLLVGQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQ 270
            :|||||:|||||||:|||||..||:||.:|||.||.:|.::...||.::..|||.:|.:||.|.:
Mouse   234 VWSIGCIMYTLLVGKPPFETSCLKETYLRIKKNEYSIPKHINPVAASLIQKMLQTDPTARPTIHE 298

  Fly   271 LLNFEFLKGSKVPMFLPSSCLTMAPRIGSNDTIEDSMHRKPLMEMN-GIR---PDDTRLESTFLK 331
            |||.||.....:|..||.:|||:.||.....:..|...||||..:| |:.   ||..|.:...:.
Mouse   299 LLNDEFFTSGYIPARLPITCLTIPPRFSIAPSSLDPSSRKPLKVLNKGVENPLPDRPREKEEPVV 363

  Fly   332 ANLHDAITASAQVCRHSEDYRSDIESLYQQLTNLINGKPR----ILQGNLGDENTDPAAQPLFWI 392
            ...::||          |.:.||   |.||||::...||.    :.|    :|..|||..|:||:
Mouse   364 RETNEAI----------ECHLSD---LLQQLTSVNASKPSERGLVRQ----EEAEDPACIPIFWV 411

  Fly   393 SKWVDYSDKYGFGYQLCDEGIGVMFNDTTKLILLPNQINVHFIDKDGKETYMTTTDYCKSLDKKM 457
            |||||||||||.||||||..:||:|||:|:|||..:..::.:|::||.|:|:|.:.:..||.||:
Mouse   412 SKWVDYSDKYGLGYQLCDNSVGVLFNDSTRLILYNDGDSLQYIERDGTESYLTVSSHPNSLMKKI 476

  Fly   458 KLLSYFKRYMIEHLVKAGANNVNIESDQISRMPHLHSWFRTTCAVVMHLTNGSVQLN-FSDHMKL 521
            .||:||:.||.|||:|||||....|.|:::|:|:|.:||||..|:::||:||:||:| |.||.||
Mouse   477 TLLNYFRNYMSEHLLKAGANITPREGDELARLPYLRTWFRTRSAIILHLSNGTVQINFFQDHTKL 541

  Fly   522 ILCPRMSAITYMDQEKNFRTYRFSTIVENGVSKDLYQKIRYAQEKLRKML 571
            ||||.|:|:||::::::|:|||.|.:.|.|..|:|..::|||:..:.|:|
Mouse   542 ILCPLMAAVTYINEKRDFQTYRLSLLEEYGCCKELASRLRYARTMVDKLL 591

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
polo	NP_524179.2	STKc_PLK	23..277	CDD:271001	166/253 (66%)
		POLO_box_1	388..475	CDD:240561	49/86 (57%)
		POLO_box_2	490..570	CDD:240560	39/80 (49%)
Plk1	NP_035251.3	STKc_PLK1	45..309	CDD:271089	170/263 (65%)
		Activation loop. /evidence=ECO:0000250	194..221		24/26 (92%)
		D-box that targets the protein for proteasomal degradation in anaphase. /evidence=ECO:0000250	337..340		2/2 (100%)
		POLO_box_1	407..494	CDD:240561	49/86 (57%)
		Linker. /evidence=ECO:0000250	493..507		6/13 (46%)
		POLO_box_2	509..590	CDD:240560	39/80 (49%)
		Important for interaction with phosphorylated proteins. /evidence=ECO:0000250	538..540		1/1 (100%)