DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment polo and plk4

DIOPT Version :9

Sequence 1:	NP_001014592.1	Gene:	polo / 40232	FlyBaseID:	FBgn0003124	Length:	576	Species:	Drosophila melanogaster
Sequence 2:	XP_012814446.2	Gene:	plk4 / 100038180	XenbaseID:	XB-GENE-493608	Length:	978	Species:	Xenopus tropicalis

Alignment Length:	698	Identity:	189/698 - (27%)
Similarity:	295/698 - (42%)	Gaps:	177/698 - (25%)

- Green bases have known domain annotations that are detailed below.

  Fly    25 YKRMRFFGKGGFAKCYEIIDVETDDVFAGKIVSKKLMIKHNQKEKTAQEITIHRSLNHPNIVKFH 89
            :|.:...|||.||..|....:.|....|.|::.||.|.|....::...|:.||..|.||:|::.:
Frog    44 FKVLNLLGKGSFACVYRAQSINTGIDVAIKMIDKKAMQKVGMVQRVRNEVEIHCQLKHPSILELY 108

  Fly    90 NYFEDSQNIYIVLELCKKRSMME-LHKRRKSITEFECRYYIYQIIQGVKYLHDNRIIHRDLKLGN 153
            ||||||..:|::||:|....|.. |..|:|..:|.|.|::::||:.|:.|||.:.|:||||.|.|
Frog   109 NYFEDSNYVYLILEMCHNGEMNRFLKNRKKPFSEDEARHFMHQIVTGMLYLHSHGILHRDLTLSN 173

  Fly   154 LFLNDLLHVKIGDFGLATRIEYEGERKKTLCGTPNYIAPEILTKKGHSFEVDIWSIGCVMYTLLV 218
            |.|:..:::||.||||||:::...|:..|:|||||||||||.|:..|..|.|:||:||::||.||
Frog   174 LLLSSDMNIKIADFGLATQLKMPNEKHFTMCGTPNYIAPEIATRSAHGLESDVWSLGCMLYTFLV 238

  Fly   219 GQPPFETKTLKDTYSKIKKCEYRVPSYLRKPAADMVIAMLQPNPESRPAIGQLLNFEFLKG---- 279
            |:|||:|.|:|:|.:||...:|.:|.::.:.|.|::..:|:.||..|.::..:|:..|:.|
Frog   239 GRPPFDTDTVKNTLNKIVLADYEMPDFVSREAKDLIFQLLRKNPADRLSLSSVLDHAFMTGFSNV 303

  Fly   280 -SKV----------------PMFLPSSCLTMA-----PRIGSNDTIEDSMH------RKPLMEMN 316
             |||                ..|..||.::::     .||.|..::.:.::      :.|....|
Frog   304 QSKVMGAVEDSIDSGHATISTGFTGSSGVSISGRFQEKRILSGPSLPNKVNIFQFRDKHPAERSN 368

  Fly   317 GIRPDDTRLEST-FLKANLHDAITASAQVCRHSEDYR----SDIESLYQQLTNLINGKPRILQGN 376
            |....:|:.|:. |.:.|  ...|.:.:...||...|    ||.....|..|   .|||......
Frog   369 GGSFHNTQRENNDFSEGN--GRKTVACEDRPHSRYLRRAHSSDRSGTSQSQT---YGKPSSFSER 428

  Fly   377 LGDENTDPAAQPLFWISKWVDY---SDKYGFGYQLCDEGIGVMFNDTTKL--------------- 423
            .  .:.:..|:|    |....|   |....:|      .|..||.|...|
Frog   429 C--HSVEMLAKP----SNLKGYCTSSPPKSYG------DIPQMFTDERSLERHTSPPVKEKTPSE 481

  Fly   424 ILLP-NQINVHFIDKDGKETYMTTTDYCKSLDKKMKLLSYFKR-------------YMIEHLVKA 474
            .:.| .||.....||...||       .:.....|:|...||.             :..:..::.
Frog   482 FMCPAKQITPRSGDKCQAET-------VQQWFGAMQLNGKFKNTPDTSSVSNMGGDFYSQQTMQN 539

  Fly   475 GA-----NNVNIESDQ----------ISRMPH---------------------------LHSWF- 496
            ||     |:|..:.|.          ||::|.                           |.|..
Frog   540 GAPQYAWNDVKRKKDTDSSVESVLRGISKLPSGQHKAEKSQFGEQSKARVPQQAFGSSTLRSIIS 604

  Fly   497 ------------RTTCAVVMHLTNGSVQLNF------SDHMKLIL---CPRMSAITYMDQE---- 536
                        :|..|||..|..|.|.:.|      .:.:|.:|   |.......|...|
Frog   605 PLNAERLKPIRQKTKNAVVSILETGEVCMEFLKEQNSQERVKEVLRISCDGNLIYVYHPNEGKGF 669

  Fly   537 ----------KNFRTYRFSTIVENGVSKDLYQKIRYAQEKLRKMLEKM 574
                      :|.|:|.|.::.|.     .::|.:||.:.::.:..||
Frog   670 PLVERPPSPPENMRSYTFDSLPEK-----YWKKYQYAAKFIQLVRSKM 712

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
polo	NP_001014592.1	STKc_PLK	23..277	CDD:271001	105/252 (42%)
		POLO_box_1	388..475	CDD:240561	21/118 (18%)
		POLO_box_2	490..570	CDD:240560	24/142 (17%)
plk4	XP_012814446.2	STKc_PLK4	42..297	CDD:271088	105/252 (42%)
		POLO_box_Plk4_1	599..710	CDD:240557	23/115 (20%)
		POLO_box_Plk4_2	711..818	CDD:240558	2/2 (100%)
		POLO_box_Plk4_3	895..973	CDD:240559
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D507604at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	2	1.920