DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment gig and tsc2

DIOPT Version :9

Sequence 1:	NP_524177.1	Gene:	gig / 40201	FlyBaseID:	FBgn0005198	Length:	1847	Species:	Drosophila melanogaster
Sequence 2:	XP_021331278.1	Gene:	tsc2 / 567524	ZFINID:	ZDB-GENE-030131-2702	Length:	1841	Species:	Danio rerio

Alignment Length:	2005	Identity:	616/2005 - (30%)
Similarity:	963/2005 - (48%)	Gaps:	401/2005 - (20%)

- Green bases have known domain annotations that are detailed below.

  Fly     3 SKD--KSKFKLFLKSLPAGYVGERTLRPEFER----------------ELRPEQPVAQRCRMLK- 48
            ||:  :.|||        |..|....||..::                ||..|..:..|.||:.
Zfish     6 SKESLRDKFK--------GIFGLGPTRPHHKQADNKPSEYIITLDILMELTSEYSLNHRIRMVNH 62

  Fly    49 --ELGDTQLHNFNLDESAITILFNLTNDLIVPNKPAETRQIALSFYKRLIHTQYKNLTIMREKFF 111
              ||..|:    ..:|.|:..::....||..|.:|.|.|...|...:.:|..|.:.|..:|..||
Zfish    63 VCELAKTK----RFEEHAVEAIWKAVEDLTQPEQPPEARHAVLVLLRAIIQGQGEWLGPLRAYFF 123

  Fly   112 LVIQNHE-AREDLRHLLELLDTLTDNGKDITNFEEKIGKFMLLWIPAITEANLLTPYLDILVNLI 175
            .||.::: :.|:|...||:...||:||||||..||:|.:|:|||:    |.::.:.:|.:||||:
Zfish   124 KVILDYQPSNEELPERLEVFKALTENGKDITYLEEEIARFVLLWM----ENSMSSDFLHVLVNLV 184

  Fly   176 KFNAAHLDKDILVGIVQNACDLSCSVTVNEIGLQ-CLTILEMVIGYTIFPSEPLPQCITTLCRTV 239
            |||:.:||:::.: :||..| |.|:.|.:...:: .|.:|:.|:.|...||:.|...|.||||||
Zfish   185 KFNSCYLDENVSL-MVQKIC-LLCNRTTSSTDIEVALQVLDAVVCYNCLPSDSLTVFIITLCRTV 247

  Fly   240 NHAPYCPSSFKIMKNLLGTQLGYHSMKMMCSILNDRALYDDAHLLRGAVFHLNMNIFGSNII--F 302
            |...:|.|.:|:|:.:|||.||:.::..||.|:.:|...:||.|||||||.:.|.::|::.:  .
Zfish   248 NVKEFCESCWKLMRKVLGTHLGHSAIYTMCRIMEERVYSEDAALLRGAVFFVGMALWGAHRLPAL 312

  Fly   303 QVSPMTYATNVLTAFLRALDSRQVIVTFEVILSVRMVITK--RQLSEIIWDLICDIMSSIVSNIE 365
            :.:|    |.||.:|.:|:.....:|::|::||:..:|.|  |:|..:.||::..|:..::..|:
Zfish   313 KNTP----TLVLPSFYKAMSCANEVVSYEIVLSITRLIKKYGRELQVVTWDILLSIIDRLLQQIQ 373

  Fly   366 YYEEVNINKDRLHHLQINFHENIDCIEKLLQQDRSQILGNVERIYDLIERVADRRPEASVLALIE 430
            .....:        |::..:|.:..||:|.:|  :...|:..|.:.|:|:.||:||:||||.|:.
Zfish   374 TMGSPD--------LKVIVYELLSTIEELYEQ--NDFHGSSARFFSLVEKCADKRPDASVLTLVS 428

  Fly   431 YRSRRVTATRPDWLQVLAQFVRRYYR-MSNVNVRIKTIEALVQIMDQNRACYEEEILSRVVLVHL 494
            ||::.:...:..|||.|.:.:.:::| .|...:|||.:..|..::..||..||||::..||:..|
Zfish   429 YRAQAIQPAKDGWLQNLLKLMDKFFRNESRTMIRIKVLHILSFVLSTNRQLYEEELIEVVVIPQL 493

  Fly   495 SHIHLESSVQVRVAVARALSNFATHCDTKRCMDLLDILEALINRPFEHTRHGSSSGEGTLAEVTF 559
            ..|..:....||....:.|.:.|..|.|.....||||:|.:.:||.      |.|.||. .|:..
Zfish   494 GQIAEDRDPSVRKQATQLLVDLAEGCSTHHFSSLLDIIEKVASRPL------SCSVEGD-RELCV 551

  Fly   560 GLVNNESEISDIIAAVDGLVKVFAIKLHRLPGIHALKIFNILMDHLELHYERPKIFEHISVVRYK 624
                 ||.:.|:..|:.||:.:...||:.||..||.:::.:|:.||:|||:..........:|.|
Zfish   552 -----ESPLEDVRTAILGLLDILQSKLYSLPASHASRVYELLISHLQLHYKNKYCSAISGAIRLK 611

  Fly   625 IFAWLLKARANGSYHIGYP--EGSTEVVKFSPYLGID-----------------SPLLPQAHPTA 670
            :|.:||..||:..:.:|.|  :|:   ::||||...|                 ||  |...||.
Zfish   612 VFDFLLLMRADSLHRLGVPNKDGA---LRFSPYCHCDPGESEKRVSEKKPTGSVSP--PAGSPTP 671

  Fly   671 I----SIRR-------VCKLIVRCLEHDTDYQVFQLVIRELPKVLQNKALVQGN--DIEELANTL 722
            .    |||.       ...::::||:.:||::|.:||:.::...:|.|.|:..:  :|:.|..||
Zfish   672 AAPPSSIRTAYLPYSLAFSVLLQCLKTETDWKVLKLVLDKMSCTIQYKVLILTSPCNIDNLCATL 736

  Fly   723 LKI--NLVSNNKFKRPTDEF-----HALVLPAIASLVIYHESLQPQQHYGIITALNSRVLTGIAS 780
            ..:  :.:...:.|:..|.|     ...|:|.:.::..||..|...:...::..|...::...|.
Zfish   737 CSMVTDRIILERLKKTPDGFSLTDVQLAVVPVLTAITSYHSYLDQLRQRDLVQCLEKGLIYRCAK 801

  Fly   781 VCINTMTILILEMPEALMRKLPDVLLQMSKMSDTNALATPVLEFLSLLIHLSKHLFANFTSMHNM 845
            .|:..:|:..:|||:.:::.||.::::::.:|.|.|:|:|:|||||.|:.| .||:|||.:...:
Zfish   802 QCVVALTMCTVEMPDIMIKLLPALIVKLTHISATVAMASPMLEFLSTLVRL-PHLYANFVAEQYV 865

  Fly   846 YVFAITLPYTKPHRYDHYTVSLAHHVIAGWFLKCKLELRRNCVNYIKSSIQSNAKMLSSDIVNLN 910
            .||||:||||.|.:::.|.|||||||||.||::|:|..|::.|.||...::|||      ::..:
Zfish   866 SVFAISLPYTNPSKFNQYIVSLAHHVIAMWFIRCRLPFRKDFVQYITKGLRSNA------LLPFD 924

  Fly   911 SLNEDSSNRKRSTSLTER------------GSRNNA--------NAWNDLEMRP----------- 944
            ..:|.||.|.|||||.||            |...|:        :|.:....|.
Zfish   925 DTHEQSSFRARSTSLNERPKSLRTTKVAKQGPSANSPVKDLKDLSAMDAFRSRSISVSEHAVRRM 989

  Fly   945 ------------------QMNNGLRNFHAELAETCFDFLARHTYSPCPSMPKRLPAAEFLLKDGV 991
                              |.::.|:..|.||.|||.|.:||:.:|...::|||.|.|:|||..|.
Zfish   990 QTSSTTCSLGSADENAVMQADDALKTVHLELTETCLDMMARYVFSNFSALPKRSPIADFLLSGGP 1054

  Fly   992 SQTWLVGNNLVTITTSGCPSAPARSGLCERCAQLGKAPSISLNSKSLSDAAPPLSPERERRYTKV 1056
            |.||||||.||||||||.....|..||             .:..:..........|....|.||
Zfish  1055 SMTWLVGNKLVTITTSGGSRTQALLGL-------------DMTERHGGGEMTRSDPSLHTRQTK- 1105

  Fly  1057 SLQHSSGNESAGSTELTSSSSSNS-------AAAGGHPHRQISNSSTASLDALSRRGSNPEALGS 1114
                    |:....|..||...|:       :.:|||..|      .....:||...::||  |.
Zfish  1106 --------EAPAKLESQSSQQINTNTRTRVRSISGGHALR------AGPAQSLSPLVASPE--GE 1154

  Fly  1115 ALGEGAHTGSNTSLLGNSLSQSSVSMSPSSGSVVQQPVC---------VRACT-GWAEVLVRRPT 1169
            ..|.....|....:|.   ||..:...|...:....|..         |...| ||||:.:|||:
Zfish  1155 YSGPLPPPGPPLDVLS---SQRGMEEHPPPSACNPSPPLKHKHSLAEFVPMLTQGWAEIFIRRPS 1216

  Fly  1170 GNVSWITRIQNPITNDCFGQE---LPFNNVVSLFLPTAHGGVFGPDNTIQAPPHTLADPEPEPE- 1230
            ||.||:..::||.:.  |..|   ||...:.|:.:  |..||..|.   ...|..::.|:.:|.
Zfish  1217 GNTSWLMCLENPPSP--FSSELGNLPLQELSSVLM--AMDGVKEPP---AERPTDVSQPQGKPAP 1274

  Fly  1231 -------AEVNAATAPQASALRSRMVAKARALQRQEEIHSVGGNGNGNGNG-------------- 1274
                   |.:::..:.::|....|.::.|.::..:|...|:..........
Zfish  1275 IQRSNTVASLSSLCSSESSGRFHRSISWADSVVMEESGRSMASVSPAESKEVEEFEAVPSEPIFM 1339

  Fly  1275 NGSGNAP----------------------STGGAAAIPIPRVPASGKRGKEAALCGSVSDGEADD 1317
            |.|..||                      |.||   |||.:.|.|        ||   :.| |.|
Zfish  1340 NTSTTAPGMMSRSSSTSSQDDDKSTLEEVSEGG---IPIDQPPLS--------LC---TPG-AQD 1389

  Fly  1318 DSLAFEDAQSRARNPVRRVNSSPEMSSSWRQSFLTNKPTPLSQEPVKTTADEPQSLLTLKKKTVQ 1382
            ..|:|..:.| :.:.:.:.:||||: .:..::|  :|.:..|.:|..|:          |..|||
Zfish  1390 PELSFTQSSS-SSSTLNKSSSSPEL-QTLPEAF--SKASSQSVDPAPTS----------KIPTVQ 1440

  Fly  1383 YSTKDMRVSCEAIPEEIAGSTPPSQAAALALQPESGTLPPKQHSADDVSSHVAGGSNLQAGGSTL 1447
                    :|...|.|.||||             :||      |....|..|:..|.     |.:
Zfish  1441 --------ACVEAPGESAGST-------------AGT------SCSSSSVSVSSSST-----SRM 1473

  Fly  1448 KLGKPPLSPGQPPLLGTGRVTSFGGTSVPQPGALAKSSSSGSNGTNVGVITSDYDNGNNGNGDMM 1512
            :|..||:..|  ||..||........||..|.:
Zfish  1474 RLEFPPVQTG--PLSPTGHRPRGHTISVSAPSS-------------------------------- 1504

  Fly  1513 RGRSKTISVVREVNNGNTRPPPASSFRNFGAAKPPINTKLCMNPSFIFMQLYTTGQLG-VTDEPL 1576
            |...||:.  |:...|.|....:|.                ::|||:|:|||.:...| ..::||
Zfish  1505 RRDRKTLE--RDARGGATNVEKSSG----------------LSPSFVFLQLYHSPFFGNEANKPL 1551

  Fly  1577 KVGPEN---SSAVSLIDLVPPFETHKIGVLYVGQGQCNNEVEILRNSHGSARYVEFLRNIGTLVS 1638
            .: |::   ..||.::|.:||::||||||::||.||.||||.||.|.:||.||.:||..:|.|:.
Zfish  1552 LL-PKSQLIDRAVKVLDQMPPYDTHKIGVVFVGAGQANNEVSILSNEYGSKRYAQFLTGLGKLIH 1615

  Fly  1639 LKEAEQNNLFI-MLDRNGADGKFAYIWKDDILQVTFHVATLMPTNLQDDPNCNEKKSHIGNDFVK 1702
            ||:.:.:.:|: .||:.|.||:|.|.|.|||:|..||:||||| |.:.|..|..||.|||||||.
Zfish  1616 LKDCDPDQIFLGGLDQYGDDGEFTYCWHDDIMQAIFHIATLMP-NRESDRGCCNKKRHIGNDFVV 1679

  Fly  1703 IIYNESGEEYNLNTISGQFNYACVIVEPLDLNSNRVYVKARSEISKFVCHAEYRIVSDRSAPLLA 1767
            ::||:|||:|.|.||.||||:..|:::|||..||.|.::.|.::...|..:..:|||||:.|||.
Zfish  1680 VVYNDSGEDYKLGTIKGQFNFVEVLIKPLDYESNLVTLQCRKDLEGLVDTSVAKIVSDRNLPLLV 1744

  Fly  1768 RQMALHANLASLVYQSVQKKHP---YASNWLERLRKLKRLRSKLIEGLNSQQKSG 1819
            ||||||||:||||:|.  :.:|   |||.||.|||.:||:|::..|.:.|:...|
Zfish  1745 RQMALHANMASLVHQC--RANPSDAYASKWLARLRHIKRIRTRAQEEIQSRPSHG 1797

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
gig	NP_524177.1	DUF3384	40..467	CDD:288697	146/436 (33%)
		HEAT repeat	484..515	CDD:293787	8/30 (27%)
		Tuberin	566..882	CDD:281532	110/354 (31%)
		Rap_GAP	1617..1802	CDD:280332	96/188 (51%)
tsc2	XP_021331278.1	DUF3384	54..469	CDD:314694	146/438 (33%)
		Tuberin	553..902	CDD:308901	110/354 (31%)
		Rap_GAP	1594..1780	CDD:307998	96/188 (51%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170577688
Domainoid	1	1.000	192	1.000	Domainoid score	I3168
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H462
Inparanoid	1	1.050	716	1.000	Inparanoid score	I623
OMA	1	1.010	-	-		QHG47539
OrthoDB	1	1.010	-	-		D341431at2759
OrthoFinder	1	1.000	-	-		FOG0005485
OrthoInspector	1	1.000	-	-		oto40051
orthoMCL	1	0.900	-	-		OOG6_104155
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X5077
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	13	12.810