DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Ac76E and Adcy8

DIOPT Version :9

Sequence 1:	NP_001246838.1	Gene:	Ac76E / 40180	FlyBaseID:	FBgn0004852	Length:	1312	Species:	Drosophila melanogaster
Sequence 2:	NP_058838.1	Gene:	Adcy8 / 29241	RGDID:	2036	Length:	1248	Species:	Rattus norvegicus

Alignment Length:	1299	Identity:	396/1299 - (30%)
Similarity:	592/1299 - (45%)	Gaps:	355/1299 - (27%)

- Green bases have known domain annotations that are detailed below.

  Fly    67 LNDLYTRY--RQRLRKSLFRSGL--LTSLLACVV-------------SIIIGIVYGQHLVQTMLL 114
            |..||.||  .||.:..:..:.|  ||.|...|:             .|::|...|..:|...|:
  Rat   163 LERLYQRYFLGQRRKSEVVMNVLDVLTKLTLLVLHLSLASAPMDPLKGILLGFFTGIEVVICALV 227

  Fly   115 VLAALISGSILTALQFPAVLSSPAAALAFAIVTT--FSLGTIAAITGDELAPLPMYALF--LCIH 175
            |:....:..  |.||:..|::       :..:||  .:.|....:.||.:.    |.||  ...:
  Rat   228 VVRKDTTSH--TYLQYSGVVT-------WVAMTTQILAAGLGYGLLGDGIG----YVLFTLFATY 279

  Fly   176 SMLPISWPVSVVLALFMTAIHIVYRIGTSPDYAPNLPM-----LFGEIVMLASASVSGLYYRIMS 235
            ||||:....:::..|..:.:.:..::     ..|.|.:     :..::|:....:.:|::...:|
  Rat   280 SMLPLPLTWAILAGLGTSLLQVTLQV-----LIPRLAVFSINQVLAQVVLFMCMNTAGIFISYLS 339

  Fly   236 DAAHNRTVDGTRTGIEQRVKLECEREQQEQLLLSVIPAYIAAEVKRSIMLKMADACQRAGGQAST 300
            |.|..:....||..:|.|::||.|.::||:|:|||:|.::..|:...:.....:..|.
  Rat   340 DRAQRQAFLETRRCVEARLRLETENQRQERLVLSVLPRFVVLEMINDMTNVEDEHLQH------- 397

  Fly   301 SATRFHELHVQRHTNVTILFADIVNFTPLSSSLTASDLVKTLNDLFGRFDQIAQENQCLRIKILG 365
               :||.:::.|:.||:|||||:..||.||::|:|.:||:.||:||.|||::|.|:.||||||||
  Rat   398 ---QFHRIYIHRYENVSILFADVKGFTNLSTTLSAQELVRMLNELFARFDRLAHEHHCLRIKILG 459

  Fly   366 DCYYCVSGLPISRPQHATNCVNMGLQMIDAIRHVREATGINVDMRIGIHTGNVLCGVLGLRKWQF 430
            ||||||||||..|..||..||.|||.||..||.||..|..:||||||||:|:|||||||||||||
  Rat   460 DCYYCVSGLPEPRQDHAHCCVEMGLSMIKTIRFVRSRTKHDVDMRIGIHSGSVLCGVLGLRKWQF 524

  Fly   431 DVWSDDVTLANHMESGGVAGRVHITKQTLDFLGDKFEVEQGEGGNRDAYLADHKVESYLIVPPKP 495
            ||||.||.:||.:||||:.||:||:|.|||.|...:.||:|.|..|:.:|..|.:|:|||..|
  Rat   525 DVWSWDVDIANKLESGGIPGRIHISKATLDCLSGDYNVEEGHGKERNEFLRKHNIETYLIKQP-- 587

  Fly   496 AYTYSVPRVVECIEQNDPSPTTEETKEIKETDQSHEATDVADVLLPVTVAPPPAIVDEKMSPTSI 560

  Rat   588 ----------------------------------------------------------------- 587

  Fly   561 NSQEAPLHAPLASAASMSIKELSEEEDEADEATAVTEPLMHRDQDGKNDKEPKANGGHRGSGDSA 625
              :|:.|..|               ||...|:.:.::                    .|.||.:.
  Rat   588 --EESLLSLP---------------EDIVKESVSCSD--------------------RRNSGATF 615

  Fly   626 ASESVAKSAALSLPADDLLSMSGSESGISNSGAQAQSSNPASVTPTAAAPAGGAASNSLTVAEAP 690
            ...|.:.    .||.|:::       |..|:.| |.:.|..::.|...|.|       |.|...|
  Rat   616 TEGSWSP----ELPFDNIV-------GKQNTLA-ALTRNSINLLPNHLAQA-------LHVQSGP 661

  Fly   691 ERSRRKLSVQGLMSFADRRRSSGAFIEGRKLSIHSGESFRSHAGHVTRNRPSSKMTKYVECWGAD 755
            |...:::.                    ..:.:.||:..|..
  Rat   662 EEINKRIE--------------------HTIDLRSGDKLRRE----------------------- 683

  Fly   756 RPFANIAESKLVKNIGLASIAMIESNLLPPERKCFNFNFFGPPTELKPFTMWYRNTPREAMYRAQ 820
                                                        .:|||::.::::..|..|...
  Rat   684 --------------------------------------------HIKPFSLMFKDSSLEHKYSQM 704

  Fly   821 PDTHFRFDLICAFVLFLSLAVVQLIVIELNLALLGS--LLASFVSLALFLYLSNMSVPDVHASTT 883
            .|..|:.:|:|||::.|.:..:|        :||.|  |:...:..::.:.|.:..|
  Rat   705 RDEVFKSNLVCAFIVLLFITAIQ--------SLLPSSRLMPMTIQFSILIMLHSALV-------- 753

  Fly   884 ERNGPGQVVASSRYLRLAMFVVVNILISSCAVFSVINYTVPDGVSKEPSSNQTILESN---FSSV 945
                  .:..:..|..|.:     ||..:|...                 |:|.|..|   |:|:
  Rat   754 ------LITTAEDYKCLPL-----ILRKTCCWI-----------------NETYLARNVIIFASI 790

  Fly   946 FVNSTLEDVQ-LW-EIDYAIPI-------APVFLYCCA----------ISLAAISAFLRSGFILK 991
            .:|.....:. || :.|.:||:       :.||...|:          :::...:.|||...:||
  Rat   791 LINFLGAVINILWCDFDKSIPLKNLTFNSSAVFTDICSYPEYFVFTGVLAMVTCAVFLRLNSVLK 855

  Fly   992 LIAML--VAVIAQVTVLGYSDLFEMYNDANITHG----LPLEIKGFLLLLVIILVLHTLDRQGEY 1050
            |..:|  :|:.|.:|...|:.||..|:  |:.|.    |..:....||:.:.:|.:....:|.||
  Rat   856 LAVLLIMIAIYALLTETIYAGLFLSYD--NLNHSGEDFLGTKEASLLLMAMFLLAVFYHGQQLEY 918

  Fly  1051 VARTDFLWKAKLKVEQEEVETMRGINKILLENILPAHVATHFLHLER-STELYHESYSCVAVMFA 1114
            .||.||||:.:.|.|..|::.:|..|:.:|.||||.|||.|||..:| :.|||.:||..|.||||
  Rat   919 TARLDFLWRVQAKEEINEMKDLREHNENMLRNILPGHVARHFLEKDRDNEELYSQSYDAVGVMFA 983

  Fly  1115 SIPNYKEFYDETDVNKQGLECLRLLNEIICDFDKLLLKPKFSGIEKIKTIASTYMCASGLRPGKE 1179
            |||.:.:||.:|::|.||:||||||||||.|||:||.:.:|..|||||||.||||..|||.|.|:
  Rat   984 SIPGFADFYSQTEMNNQGVECLRLLNEIIADFDELLGEDRFQDIEKIKTIGSTYMAVSGLSPEKQ 1048

  Fly  1180 DGATSRSFADEKRTEEHNVVILVEFAIALMSILDSINRESFQRFRLRIGLNHGPVIAGVIGAQKP 1244
            .       .::|   ..::..|.:|::||...:..||:.||..|.||||::||.|:||||||:||
  Rat  1049 Q-------CEDK---WGHLCALADFSLALTESIQEINKHSFNNFELRIGISHGSVVAGVIGAKKP 1103

  Fly  1245 QYDIWSNTVNVASRMDSCGVMGRLQTTENTAKILMTAGYECECRGLTYVKG----KGNLVTYFV 1304
            |||||..|||:||||||.||.||:|..|.|..||...|:..:.||..||||    :|.:.|||:
  Rat  1104 QYDIWGKTVNLASRMDSTGVSGRIQVPEETYLILKDQGFAFDYRGEIYVKGISEQEGKIKTYFL 1167

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Ac76E	NP_001246838.1	AC_N	<67..289	CDD:292831	57/247 (23%)
		CYCc	259..467	CDD:214485	113/207 (55%)
		Guanylate_cyc	311..469	CDD:278633	101/157 (64%)
		BASP1	510..667	CDD:283191	18/156 (12%)
		CYCc	1079..1283	CDD:214485	111/204 (54%)
		Guanylate_cyc	1101..1305	CDD:278633	109/208 (52%)
Adcy8	NP_058838.1	Involved in ORAI1, STIM1, PPP2CA and PPP2R1A interaction. /evidence=ECO:0000269\|PubMed:16258073, ECO:0000269\|PubMed:22494970, ECO:0000269\|PubMed:22976297	1..179		7/15 (47%)
		Involved in AKAP5 and PRKAR2A interaction. /evidence=ECO:0000269\|PubMed:22976297	1..106
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	38..40
		Essential for CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	49..51
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	51..117
		AC_N	<158..397	CDD:292831	57/251 (23%)
		CYCc	363..561	CDD:214485	113/207 (55%)
		Guanylate_cyc	402..586	CDD:278633	112/183 (61%)
		DUF1053	614..708	CDD:283888	25/199 (13%)
		CYCc	941..1145	CDD:214485	114/213 (54%)
		Guanylate_cyc	970..1169	CDD:278633	109/208 (52%)
		Involved in CALM1 interaction. /evidence=ECO:0000269\|PubMed:16258073	1106..1248		32/62 (52%)
		Required for both calcium stimulation and maintenance of autoinhibition. /evidence=ECO:0000269\|PubMed:19305019	1197..1212
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1220..1248
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG2114
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100296
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.710