DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Usp32 and UBP12

DIOPT Version :9

Sequence 1:	NP_001262074.2	Gene:	Usp32 / 40169	FlyBaseID:	FBgn0036913	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_012338.1	Gene:	UBP12 / 853242	SGDID:	S000003733	Length:	1254	Species:	Saccharomyces cerevisiae

Alignment Length:	1487	Identity:	315/1487 - (21%)
Similarity:	493/1487 - (33%)	Gaps:	557/1487 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly   419 YTQHTTHTCDYCKRTASQRTAVDEALVCD-------ESFNTHSLEQHDSYSLGSGTGSASGSGSA 476
            :|..|| .||        |..||...|.|       ||......|..|:.|..|...|.:
Yeast    21 FTDGTT-PCD--------RLGVDLMNVLDDKDEIKQESVPVSDREIEDTESDASAVSSFA----- 71

  Fly   477 SSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFE---------- 531
                               ::|.:.|.|.....|..|..|...|:. |.|..|..
Yeast    72 -------------------SANELIAEPHAASETNLGTNGQDGRNV-LEQQRDVVARLIEENKET 116

  Fly   532 --------LVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRIL------------- 575
                    :|||.       ||.       :...|..:|.| ::.|:|.|::
Yeast   117 QKEGDKVCIVPKV-------WYD-------KFFDPDVTDPE-DIGPINTRMICRDFENFVLEDYN 166

  Fly   576 ----LHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPP------------ 624
                |..|:|.             .:.:|..||:  :.|.|..:....:.|..
Yeast   167 RCPYLSIAEPV-------------FNFLSEIYGM--TSGSYPVVTNLVINQTTGELETEYNKWFF 216

  Fly   625 ------------KR---------YLAYTAAFSRLATVRQVGE-----FL--CEQLRLKSEDIRLW 661
                        ||         ||:.:|    |..||.:.|     |.  .:.|.:.:.|.::|
Yeast   217 RLHYLTEKQDGRKRRHGQDDSIMYLSMSA----LNLVRDLVEKSMNLFFEKADHLDVNAVDFKIW 277

  Fly   662 HVPQ------LDNGAILLEED---------AMCLKELLIRD------------NDQLLLEIR--N 697
            .|.:      ..|.:..|...         .:.:|:|||.|            ...|::||:  .
Yeast   278 FVSEGSDIATDSNVSTFLNSSYEITPLQFLELPIKKLLIPDMFENRLDKITSNPSDLVIEIKPIE 342

  Fly   698 KDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNT 762
            .:..||....:....:                      .|.|.|||.||||||:||:|||.|.:.
Yeast   343 GNHHWPSNYFAYNKLE----------------------PASGTTGLVNLGNTCYMNSALQCLVHI 385

  Fly   763 QPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVW----TATTRSVA--PLKLRFCVNKY 821
            ..|..||..:.:..|:|..|.||..|.:|..:::|:::::    :...|:.|  |...:..:..:
Yeast   386 PQLRDYFLYDGYEDEINEENPLGYHGYVARAFSDLVQKLFQNRMSIMQRNAAFPPSMFKSTIGHF 450

  Fly   822 APQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPD-------KIVAAEAWSQHH 879
            ...|:|..|.||||.|.:|||:|||||||:::|.|:|....:...|       |.:|.:.|..|.
Yeast   451 NSMFSGYMQQDSQEFLAFLLDSLHEDLNRIIKKEYTEKPSLSPGDDVNDWNVVKKLADDTWEMHL 515

  Fly   880 ARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYG 944
            .||.|:|.|||.|..||.:.|..|.:.|:.|||::.::|||||:. ::.:.:.|....|.|:...
Yeast   516 KRNCSVITDLFVGMYKSTLYCPECQNVSITFDPYNDVTLPLPVDT-VWDKTIKIFPMNSPPLLLE 579

  Fly   945 FRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQI----------RQVLNDDEKLRTQSAKEL 999
            ..|:....|..||:.:..|..|.||.:..||::::||          .|.|...|.::  .|.::
Yeast   580 VELSKSSTYMDLKNYVGKMSGLDPNTLFGCEIFSNQIYVNYESTESNAQFLTLQELIK--PADDV 642

  Fly  1000 YVYQLPEQSMRTRSNS----GLSMHIEQGLKDIQRSSA--LITSAQDSLSSLSTLQTSSHRASSR 1058
            ..|:||.    |..|.    .|:..||:|.|:......  .||..:|.|::...::.   :..:|
Yeast   643 IFYELPV----TNDNEVIVPVLNTRIEKGYKNAMLFGVPFFITLKEDELNNPGAIRM---KLQNR 700

  Fly  1059 VLCNGHVSGLDVEGEAEVG--TDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCF 1121
            .:   |:||..:.....||  ||.:..     :..:|..|              ||...:.
Yeast   701 FV---HLSGGYIPFPEPVGNRTDFADA-----FPLLVEKY--------------PDVEFEQ---- 739

  Fly  1122 GKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSME 1186
                        :|:.|..:|..       ...:.|...|.|:|..|......| .|.:|.|
Yeast   740 ------------YKDILQYTSIK-------VTDKDKSFFSIKILSVEKEQQFAS-NNRTGPN--- 781

  Fly  1187 SSLTEPIPLADLEPVSS------------RNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQ 1239
              ...||...:|:..:.            .|.|...||:.                |..:.||::
Yeast   782 --FWTPISQLNLDKATDIDDKLEDVVKDIYNYSSLVDCAE----------------GVLMQVDDE 828

  Fly  1240 AE-EGN-AEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTR 1302
            .: ||: |::..:|.|....:....:                        :|.:::.
Yeast   829 GDTEGSEAKNFSKPFQSGDDEENKET------------------------VTNNENV-------- 861

  Fly  1303 PSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVK 1367
                       |:.....:|:  .:...|....|..|..|::........||| ...||.:.::.
Yeast   862 -----------NNTNDRDEDM--ELTDDVEEDASTEPELTDKPEALDKIKDSL-TSTPFAILSMN 912

  Fly  1368 ADGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEY 1432
                ...:|.||..  |.....::|.:.            |...|      .:|||.|.:
Yeast   913 ----DIIVCEWSEL--GSNEAFSDDKIY------------NWENP------ATLPNKELE----- 948

  Fly  1433 TASLSYTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAF 1497
                                           .:.|||......||.           |:.||:.|
Yeast   949 -------------------------------NAKLERSNAKERTIT-----------LDDCLQLF 971

  Fly  1498 TSEEKL---EQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDF 1559
            :..|.|   :.|| |..||..:.|||::|:|..|.||::|||||........|....|:||..|.
Yeast   972 SKPEILGLTDSWY-CPTCKEHRQATKQIQLWNTPDILLIHLKRFESQRSFSDKIDATVNFPITDL 1035

  Fly  1560 DPTPYLASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATA 1624
            |.:.|:.
Yeast  1036 DLSRYVV---------------------------------------------------------- 1042

  Fly  1625 SPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPR---YRLYAV 1686
                                                 ::|             |||   |.||||
Yeast  1043 -------------------------------------YKD-------------DPRGLIYDLYAV 1057

  Fly  1687 VSHSGMLNGGHYISYASN-ATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLD 1742
            .:|.|.|.||||.:|..| |...||.::||...|.:.:..| ..:||||||.|:..|
Yeast  1058 DNHYGGLGGGHYTAYVKNFADNKWYYFDDSRVTETAPENSI-AGSAYLLFYIRRHKD 1113

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	FRQ1	186..317	CDD:227455
		UBP12	486..>1002	CDD:227847	153/642 (24%)
		Peptidase_C19	<1480..1737	CDD:351799	61/263 (23%)
UBP12	NP_012338.1	UBP12	99..1111	CDD:227847	290/1371 (21%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	273	1.000	Domainoid score	I273
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	279	1.000	Inparanoid score	I567
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm46646
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR21646
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	6	6.060