Protein Alignment Usp32 and DOA4

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_010354.3
• Gene: DOA4 / 851641
• SGDID: S000002476
• Length: 926
• Species: Saccharomyces cerevisiae
• Alignment Length: 1067
• Identity: 215/1067 - (20%)
• Similarity: 358/1067 - (33%)
• Gaps: 397/1067 - (37%)

  Fly   106 CNDAGTFI-IKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSD--NCV 167
            |.:.|..| ..|..:..:|...::.:.:...:...:..:|..  ||...:|       ||  |..
Yeast    75 CKNKGAMISSNSVMIIQLNYVYYKVIHIIVTTNIPHLSEFAK--IKLHKST-------SDEGNGN 130

  Fly   168 SLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWR--LKNTSQN---GQIDLQFLGPLISPPIPK 227
            :..:|.:....|.:|. .|.|::::|..: |.|.:  :|.|..|   .:.:|...|..|:    .
Yeast   131 NNNNEFQLMNIYNTLL-ETLLKDENIAKI-KSFIKSSIKQTKLNHEQEECNLMRTGSYIT----S 189

  Fly   228 NALAGLFNA-------------FD-----ENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFD 274
            |.|..|.::             .|     |....|||.|.:.|          :|...|.....|
Yeast   190 NQLNSLISSSANSASSQMEILLIDIRSRLEFNKSHIDTKNIIC----------LEPISFKMSYSD 244

  Fly   275 VDRDGVLSHDETLQMINVLLLVAKENQESQ--QYKDLTKQLVI-SDLLEFGQRRSPDGTPSKLTR 336
                    ||     :....|:...|.|.:  |.::|.|.::: :|..|:
Yeast   245 --------HD-----LEKKSLITSPNSEIKMFQSRNLFKFIILYTDANEY--------------- 281

  Fly   337 DNVSLTAEDFMLWTVQCDLRLMQPLLDLI----FELCHIVFGLWPQCKHMENDIVR--------- 388
                             :::....|||::    ||            |.:.:|..:
Yeast   282 -----------------NVKQQSVLLDILVNHSFE------------KPISDDFTKIFILESGFP 317

  Fly   389 GWLRREERRPYRVGQFW-----------YLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVD- 441
            |||:....|  :|...:           |:..:...||    .||..      |.:.|.|.::|
Yeast   318 GWLKSNYGR--QVSSSFPSNNNIKDDSVYINGNTSGLS----LQHLP------KMSPSIRHSMDD 370

  Fly   442 ---EALVC------------DESFNTHSLEQHDSY-SLGSGTGSASGSGSAS-----SGISAGRH 485
               |.||.            .:|.|.|.|::..|: .|.|...|.:...|.|     |.:|....
Yeast   371 SMKEMLVAPTPLNHLQQQQQQQSDNDHVLKRSSSFKKLFSNYTSPNPKNSNSNLYSISSLSISSS 435

  Fly   486 CGPV---RPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGD 547
            ..|:   .|.|:..::|....|                :||            .|||....   |
Yeast   436 PSPLPLHSPDPVKGNSLPINYP----------------ETP------------HLWKNSET---D 469

  Fly   548 NLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGY 612
            .:...|:.:   |.:....:.|:|.:.:   ..||:|..   .:|..:..|:|....:.::|...
Yeast   470 FMTNQREQL---NHNSFAHIAPINTKAI---TSPSRTAT---PKLQRFPQTISMNLNMNSNGHSS 525

  Fly   613 AAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDA 677
            |    :|.:||
Yeast   526 A----TSTIQP------------------------------------------------------ 532

  Fly   678 MCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATG 742
            .||.   :.:||.|                               |...:|.:|..:.....|.|
Yeast   533 SCLS---LSNNDSL-------------------------------DHTDVTPTSSHNYDLDFAVG 563

  Fly   743 LHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELL-----KEVW 802
            |.||||:|:||..:|.:..|..|.|.|..:.:...:|..:|||:||.||..:|.|:     ::|.
Yeast   564 LENLGNSCYMNCIIQCILGTHELTQIFLDDSYAKHININSKLGSKGILAKYFARLVHMMYKEQVD 628

  Fly   803 TATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRV-MEKPYSEL-KDSNGR 865
            .:...|::|:|.:.........|....|.|.||..::|||.||||||:. ...|..|| :::..|
Yeast   629 GSKKISISPIKFKLACGSVNSLFKTASQQDCQEFCQFLLDGLHEDLNQCGSNPPLKELSQEAEAR 693

  Fly   866 PDK----IVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYI 926
            .:|    |.::..|.:....:.|:|:|||.||..|::.|..|.|.|..:.||::||:|:|.:|
Yeast   694 REKLSLRIASSIEWERFLTTDFSVIVDLFQGQYASRLKCKVCSHTSTTYQPFTVLSIPIPKKN-- 756

  Fly   927 YFEVLVILLDGSVPIKYGFR--------------LNSDCKYSHLKHKLSTMCSLPPNLML----- 972
                    ...::.|:..||              |...|:......|..|:..||.||::
Yeast   757 --------SRNNITIEDCFREFTKCENLEVDEQWLCPHCEKRQPSTKQLTITRLPRNLIVHLKRF 813

  Fly   973 ------------------VCELWNSQIRQV----LNDDE-KLRTQSAK---ELYVYQLPEQSMRT 1011
                              :...|.:....|    :|||| .:|.|...   |||
Yeast   814 DNLLNKNNDFVIYPFLLDLTPFWANDFDGVFPPGVNDDELPIRGQIPPFKYELY----------- 867

  Fly  1012 RSNSGLSMH------------IEQGLK 1026
                |::.|            :::|||
Yeast   868 ----GVACHFGTLYGGHYTAYVKKGLK 890

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	17/75 (23%)
		EF-hand motif	198..222	CDD:320029	7/28 (25%)
		EF-hand_7	227..290	CDD:463900	15/80 (19%)
		UBP12	486..>1002	CDD:227847	125/574 (22%)
		Peptidase_C19	<1480..1737	CDD:470612
DOA4	NP_010354.3	RHOD	208..324	CDD:197731	30/182 (16%)
		UCH	562..920	CDD:425685	95/354 (27%)