Protein Alignment Usp32 and USP32

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_011523673.1
• Gene: USP32 / 84669
• HGNCID: 19143
• Length: 1634
• Species: Homo sapiens
• Alignment Length: 1895
• Identity: 689/1895 - (36%)
• Similarity: 975/1895 - (51%)
• Gaps: 438/1895 - (23%)

  Fly    21 VSDVELRRLRDAFKKSAGVGRNFLSRNAFQQDVLCEGVPPKIVDMLYAACGGTQRGISFNDLLCG 85
            |:||||:||:||||::.|:. .::.::.|.::||.:|||||:.:::|.:.|||.:|:.||:|:.|
Human    36 VTDVELKRLKDAFKRTCGLS-YYMGQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVG 99

  Fly    86 LVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNL----APFESVSLFAQSERVNFEQFQD 146
            |||:|||...||.|::::|:.:::|.::|:.:..|.:::    .|......|::.|:||:|:|::
Human   100 LVLLTRGKDEEKAKYIFSLFSSESGNYVIREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRN 164

  Fly   147 WIIKHRNATVLSKWLLSDNC-VSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNG 210
            |:..:::|...|:||||... |:||.:.:||||||:|||||||||.||.||||.:|.||..|:.|
Human   165 WLFLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTG 229

  Fly   211 QIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDV 275
            :.||:..|||:||||..:...|||||||||||.||||||:.||:||.||||..||.:||||:|||
Human   230 RFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDV 294

  Fly   276 DRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTK-QLVISDLLEFGQRRSPDGTPSKLTRDNV 339
            |||||||..|...|:..||.|.|:|:..    |:.: .:.:||::| |...:.|.|  |:..
Human   295 DRDGVLSRVELRDMVVALLEVWKDNRTD----DIPELHMDLSDIVE-GILNAHDTT--KMGH--- 349

  Fly   340 SLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQF 404
             ||.||:.:|:|:  ..|....|:|:|::||||.||.|.....|..|:||||.||.|...:.|..
Human   350 -LTLEDYQIWSVK--NVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQAGHN 411

  Fly   405 WYLITHDWWLSWMQYTQHTTHTC-----------DYCKRTASQ-RTAVDEALVCDESF-NTHSLE 456
            |::|:..||..|.:|.::..:..           .|...||:. ...|::.:....|: ||...:
Human   412 WFIISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKYSFGTAAHPMEQVEDRIGSSLSYVNTTEEK 476

  Fly   457 QHDSYSLGSGTGSASGSG---SASSGISA--------------------GR---HCGPVRPGPID 495
            ..|:.|..|.....:|||   ||:.|...                    |.   |..|.:||.||
Human   477 FSDNISTASEASETAGSGFLYSATPGADVCFARQHNTSDNNNQCLLGANGNILLHLNPQKPGAID 541

  Fly   496 NSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPN 560
            |..|:|..|.: ..:||.|||.|||...|:...|:|:||:.:|:||..|||.||.|||.||:...
Human   542 NQPLVTQEPVK-ATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGANLALPRPVIKNSK 605

  Fly   561 SDV-ELELYPLNLRILLHQ----AQPSQTGVG-GGTQLGSWGSTVSGGYGVLASGGGYAAIAVSS 619
            :|: ||||:|..|..|..|    .|.|...|. |...|..:...:..|             .|.|
Human   606 TDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGMMSLRMFPQHLPRG-------------NVPS 657

  Fly   620 VLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELL 684
            ...|.||.||||..|||:.|::::.|:|.::||:|.||:||| :...:|...||:::...|:.|.
Human   658 PNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLW-LYNSENYLTLLDDEDHKLEYLK 721

  Fly   685 IRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNT 749
            |:|...|::|:||||::||||:..:|.:       :..||.::....       |||||.|||||
Human   722 IQDEQHLVIEVRNKDMSWPEEMSFIANS-------SKIDRHKVPTEK-------GATGLSNLGNT 772

  Fly   750 CFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKL 814
            ||||:::|.:.|||||.|||....|.:|:|..|.:|.||.:|..|.:|::|:|:.|.::||||||
Human   773 CFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGTQKNVAPLKL 837

  Fly   815 RFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHH 879
            |:.:.||||:|.|..|.||||||.:|||.||||||||.||||.|||||:||||..||||||..|.
Human   838 RWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEVAAEAWDNHL 902

  Fly   880 ARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVPIKYG 944
            .||:||::|||:|||:|:|.|..|||.|||||||:.||||||:::|::.|:.||.|||:.|::||
Human   903 RRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKLDGTTPVRYG 967

  Fly   945 FRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSM 1009
            .|||.|.||:.||.:||.:|.|....:|:.|:..|.|:....|::|:|...:..|..:::|.
Human   968 LRLNMDEKYTGLKKQLSDLCGLNSEQILLAEVHGSNIKNFPQDNQKVRLSVSGFLCAFEIPV--- 1029

  Fly  1010 RTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEA 1074
                                                                             
Human  1030 ----------------------------------------------------------------- 1029

  Fly  1075 EVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKRECMPHSLFCFKESLI 1139
                            |:                                            |.|
Human  1030 ----------------PV--------------------------------------------SPI 1034

  Fly  1140 LSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSR 1204
            .:|||                      |:::.||...||.....:....|..||.:.
Human  1035 SASSP----------------------TQTDFSSSPSTNEMFTLTTNGDLPRPIFIP-------- 1069

  Fly  1205 NGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSRR 1269
            ||.........|..|.::|:..||.  .||                ||...|.
Human  1070 NGMPNTVVPCGTEKNFTNGMVNGHM--PSL----------------PDSPFTG------------ 1104

  Fly  1270 SSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVWLQVSRL 1334
                      |::|||||:.|.:.||||..|.||||||:||::|.:.....||||.|||:|||||
Human  1105 ----------YIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYDAVWIQVSRL 1159

  Fly  1335 LSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDYVLQGAL 1399
            .|||| ..|.:|||.|||||:||.:|||||.|:.||.:||.|||..|||||:|.|..|....|
Human  1160 ASPLP-PQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCGEDRAFIG-- 1221

  Fly  1400 PPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTALHLRYQ 1464
                           ||                                .||:||||||||||||
Human  1222 ---------------NA--------------------------------YIAVDWDPTALHLRYQ 1239

  Fly  1465 STLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL--EQWYHCSHCKGKKPATKKLQIWKL 1527
            ::.||:..:||::..|||.|.||::|:.|||||||||:|  .:.|:||.||....|||||.:|:|
Human  1240 TSQERVVDEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLATKKLDLWRL 1304

  Fly  1528 PPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETIL-RHKELL----EL----- 1582
            |||||:|||||..|||:|:||||:|.||.:.|||:.:|  ||::..| :||.|.    ||
Human  1305 PPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL--VPRDPALCQHKPLTPQGDELSEPRI 1367

  Fly  1583 ------KNDAEMTMATNEVV----------------------SELDEIDAPSKEVKEELPNQT-- 1617
                  |.||:.:....:|:                      |.......||.  |...||.:
Human  1368 LAREVKKVDAQSSAGEEDVLLSKSPSSLSANIISSPKGSPSSSRKSGTSCPSS--KNSSPNSSPR 1430

  Fly  1618 --GSTKATASPPPTGNILRQSKTK------------------NAVRRQRLISTSLTK--TP---- 1656
              |.:|.....|..|:..:.|.:|                  :|:.|..::..|..:  ||
Human  1431 TLGRSKGRLRLPQIGSKNKLSSSKENLDASKENGAGQICELADALSRGHVLGGSQPELVTPQDHE 1495

  Fly  1657 --IVDGEFEDYH------------QHRLKPDVD------QFDPRYRLYAVVSHSGMLNGGHYISY 1701
              :.:|...::.            .|..:...|      :..|.|.|||:..|||:|.||||::|
Human  1496 VALANGFLYEHEACGNGYSNGQLGNHSEEDSTDDQREDTRIKPIYNLYAISCHSGILGGGHYVTY 1560

  Fly  1702 ASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYEPYLPNIEGRTLPNTASVPLEVD 1766
            |.|....|||||||||:|: ....||..:||:||||::|:||..:||..:|:.:.:|:|:.   :
Human  1561 AKNPNCKWYCYNDSSCKEL-HPDEIDTDSAYILFYEQQGIDYAQFLPKTDGKKMADTSSMD---E 1621

  Fly  1767 ETEGELKKLC 1776
            :.|.:.||.|
Human  1622 DFESDYKKYC 1631

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	32/52 (62%)
		EF-hand motif	198..222	CDD:320029	11/23 (48%)
		EF-hand_7	227..290	CDD:463900	42/62 (68%)
		UBP12	486..>1002	CDD:227847	239/521 (46%)
		Peptidase_C19	<1480..1737	CDD:470612	118/344 (34%)
USP32	XP_011523673.1	EFh	253..309	CDD:238008	40/55 (73%)
		EF-hand_7	286..358	CDD:463900	33/82 (40%)
		UBP12	534..1346	CDD:227847	412/1090 (38%)
		UCH	<1261..1594	CDD:425685	113/337 (34%)