Protein Alignment Usp32 and Usp8

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_001239509.1
• Gene: Usp8 / 84092
• MGIID: 1934029
• Length: 1091
• Species: Mus musculus
• Alignment Length: 1138
• Identity: 237/1138 - (20%)
• Similarity: 391/1138 - (34%)
• Gaps: 351/1138 - (30%)

  Fly   112 FIIKSDYVRNVNLAPFESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETP 176
            :::...||...||.          .:|.:|:|.||:.:.......:.|.:.....:|.:.:|.
Mouse    71 YVLYMKYVAVYNLI----------KKRPDFKQQQDYYLSILGPANIKKAIEEAERLSESLKLR-- 123

  Fly   177 TFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENR 241
              |:.......|||||  ..|:|..:.:...:.|:.|        |....|.::..|.::..:.:
Mouse   124 --YEEAEVRKQLEEKD--RREEEQLQQQKRQEMGRED--------SGAAAKRSVENLLDSKTKTQ 176

  Fly   242 DGHIDFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQY 306
            ..:.:..|   |.:||.||....:..:                 |:.|.....|:..:.::.|.|
Mouse   177 RINGEKSE---GAAAAERGAITAKELY-----------------TMMMDKNTSLIIMDARKIQDY 221

  Fly   307 KDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVS----------------------LTAEDFMLW 349
                :...|.|.|...:.....|..:.....|:|                      .:|:|.:|.
Mouse   222 ----QHSCILDSLSVPEEAISPGVTASWIEANLSDDSKDTWKKRGSVDYVVLLDWFSSAKDLLLG 282

  Fly   350 TVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWL 414
            |.      ::.|.|.:|:        |      |:..|   ||.|.           |:....:.
Mouse   283 TT------LRSLKDALFK--------W------ESKTV---LRHEP-----------LVLEGGYE 313

  Fly   415 SWMQ-YTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLE---------------------- 456
            :|:. |.|.||:    .|.|...|:..:|..| ...|...|||
Mouse   314 NWLLCYPQFTTN----AKVTPPPRSRAEEVSV-SLDFTYPSLEEPVPSKLPTQMPPPPIETNEKA 373

  Fly   457 -----QHDSYSLG-----SGTGSA--------------------------------------SGS 473
                 |.:...|.     :|.|:|                                      |.:
Mouse   374 LLVTDQDEKLRLSTQPALAGPGAAPRAEASPIIQPAPATKSVPQVDRTKKPSVKLPEDHRIKSEN 438

  Fly   474 GSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLT-------------------------- 512
            ...|..:.:.|...||.|.|   :.::|......:...|
Mouse   439 TDQSGRVLSDRSTKPVFPSP---TTMLTDEEKARIHQETALLMEKNKQEKELWDKQQKEQKEKLR 500

  Fly   513 -------------------------GEGGHLKRDTPLVQNHDFELVPKSLWKAL-------NRWY 545
                                     .:.|..|:|:...:..|.||......:|.       :...
Mouse   501 REEQERKAGKTQDADERDSTENQHKAKDGQEKKDSKQTKTEDRELSADGAQEATGTQRQSKSEHE 565

  Fly   546 GDNLPLPRQVIQPPNSDVE---LELYPLNL----------RILLHQAQPSQTG-VGGGTQLGSWG 596
            ..:..:|.:..:.|.|:.:   .::.|.::          |..|.:|:..:.| :..|..|| |.
Mouse   566 ASDAKVPVEGKRCPTSEAQKRPADVSPASVSGELNAGKAQREPLTRARSEEMGRIVPGLPLG-WA 629

  Fly   597 STVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLW 661
            ..:....|...    |.....::|...|......:|..|...|.:...:...|:.|..|:..|.:
Mouse   630 KFLDPITGTFR----YYHSPTNTVHMYPPEMAPSSAPPSTPPTHKVKPQVPAERDREPSKLKRSY 690

  Fly   662 HVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPE-ELGSLATAQCGQGAGTPGDRR 725
            ..|.:...  |.||:..       |.....::...||...:|: |:..|:.:|.          |
Mouse   691 SSPDITQA--LQEEEKR-------RPAVTPMVNRENKPPCYPKAEISRLSASQI----------R 736

  Fly   726 RLTRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQL 790
            .|  :.:.....|..|||.||||||:||:.||.|.|...||.||.|..::.::|.:|.||.||::
Mouse   737 NL--NPVFGGSGPALTGLRNLGNTCYMNSILQCLCNAPHLADYFNRNCYQDDINRSNLLGHKGEV 799

  Fly   791 AMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRV-MEK 854
            |..:..::|.:||...|.::|...:..:.|...||||..|.||||||.:|:|.||||||:. ..|
Mouse   800 AEEFGIIMKALWTGQYRYISPKDFKVTIGKINDQFAGSSQQDSQELLLFLMDGLHEDLNKADNRK 864

  Fly   855 PYSELKDSNGRPDKIVAAE-AWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSL 918
            .:.|  ::|...|.:.||| ||.:|...|:|||:.||.||.||.|.||.|...|..|:.|..|||
Mouse   865 RHKE--ENNEHLDDLQAAEHAWQKHKQLNESIIVALFQGQFKSTVQCLTCRRRSRTFEAFMYLSL 927

  Fly   919 PLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQ 983
            ||                                      ..::.|:|...|.|..:      .:
Mouse   928 PL--------------------------------------ASTSKCTLQDCLRLFSK------EE 948

  Fly   984 VLNDDEKL-------RTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDS 1041
            .|.|:.:.       |..|.|::.:::||..         |.:|:::...|.:....|.||....
Mouse   949 KLTDNNRFYCSHCRARRDSLKKIEIWKLPPV---------LLVHLKRFSYDGRWKQKLQTSVDFP 1004

  Fly  1042 LSSLSTLQ-----TSSHRASSRVLCNGHVSGLD 1069
            |.:|...|     .:|.:..:....:.|..|||
Mouse  1005 LENLDLSQYVIGPKNSLKKYNLFSVSNHYGGLD 1037

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	7/52 (13%)
		EF-hand motif	198..222	CDD:320029	3/23 (13%)
		EF-hand_7	227..290	CDD:463900	9/62 (15%)
		UBP12	486..>1002	CDD:227847	143/597 (24%)
		Peptidase_C19	<1480..1737	CDD:470612
Usp8	NP_001239509.1	USP8_dimer	6..127	CDD:462647	13/69 (19%)
		Radial_spoke_3	<91..>171	CDD:461827	19/93 (20%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	130..187		14/69 (20%)
		Rhodanese	204..315	CDD:425764	24/148 (16%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	390..466		11/78 (14%)
		SH3-binding	416..424		0/7 (0%)
		PTZ00121	<464..589	CDD:173412	11/124 (9%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	479..616		14/136 (10%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	653..721		15/76 (20%)
		UCH	750..1079	CDD:425685	109/343 (32%)