Protein Alignment Usp32 and UBP11

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_174562.2
• Gene: UBP11 / 840179
• AraportID: AT1G32850
• Length: 892
• Species: Arabidopsis thaliana
• Alignment Length: 1417
• Identity: 311/1417 - (21%)
• Similarity: 444/1417 - (31%)
• Gaps: 623/1417 - (43%)

  Fly   391 LRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSL 455
            |..|.....:.|..:::|::.|:..|.::....|                       |.|.
plant    29 LNNEAEADLKEGNLYFVISNRWYTRWQRFVGLLT-----------------------EEFR---- 66

  Fly   456 EQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLI-----TANPFRNVRTLTGEG 515
                             ||..|.         ..|||||||.::|     .::|  .:|.:..||
plant    67 -----------------SGEPSE---------VTRPGPIDNHDIIDSESDASDP--QLRMMLEEG 103

  Fly   516 GHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPP--NSDVELELYPLNLRILLHQ 578
                        .|:.||.:.:|:.|.:||....|:||::|...  .....:|:|.|.|.:
plant   104 ------------VDYTLVQQEVWRKLVKWYKGGPPVPRKLISQGFYTKSFSVEVYLLCLTL---- 152

  Fly   579 AQPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQV 643
                       |......:|:                                ...|:.|::.|:
plant   153 -----------TDSRDESTTI--------------------------------IRLSKQASIGQL 174

  Fly   644 GEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEI------------- 695
            .|.:|....:..|..|:|...:.....:|.......::|..::.|..:|||:
plant   175 YEMVCAGKGVAKEKARIWDYFEKKKSVLLDPSSEQSVEEAGLQFNQDILLEVDGSASSQFVMSLA 239

  Fly   696 RNKDLTWPEELGSLATAQCGQGAGT-----------------------------PGDRRRLTRSS 731
            .|:....|.|..........:|.||                             .|::|
plant   240 ENELAMVPLEPMRSDAMDIVRGGGTLSNGHSNGFKFSFFGRNTFKDDVSSRTFGKGEKR------ 298

  Fly   732 IMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAE 796
                   |..||.|||||||||:.||.|.:|.|:.:||.:: :|.::||.|.||.:|:||:.:.|
plant   299 -------GLGGLQNLGNTCFMNSTLQCLAHTPPIVEYFLQD-YRSDINAKNPLGMRGELAIAFGE 355

  Fly   797 LLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKD 861
            ||:::|::...:|||...:..:.::||||:|..||||||:|.:|||.||||||:|..|||.|.||
plant   356 LLRKLWSSGQNTVAPRAFKTKLARFAPQFSGYNQHDSQEMLAFLLDGLHEDLNKVKRKPYIEAKD 420

  Fly   862 SNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYI 926
            |:||||..||.|.|..|.|||.|:|:|:|.||.||.:.|..||..|:.||||..||||||.....
plant   421 SDGRPDDEVAEEKWKYHKARNDSVIVDVFQGQYKSTLVCPDCGKISITFDPFMYLSLPLPSSRTR 485

  Fly   927 YFEVLVILLDGS-VPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEK 990
            ...|.|...||| :|:.|...:..|.....|.:.|.|.|         |          |::||.
plant   486 SMTVTVFYGDGSHLPMPYTVTVPKDGSCRDLSNALGTAC---------C----------LDNDES 531

  Fly   991 L---RTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSS 1052
            |   .....|....|:.|.:.:             .|:||
plant   532 LLLAEVYDHKVFKYYENPRELL-------------NGIKD------------------------- 558

  Fly  1053 HRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVH-ELLPDEAGK 1116
                     |.|:                          :...:.....|..:|. |:|..|..|
plant   559 ---------NEHI--------------------------VAYRFKQMHKGPGKVKLEILHGEQEK 588

  Fly  1117 VS----RCFGKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYT 1177
            .|    :|||    .|...:..||.  ||.:...|.:.|..:..:||      |.....:|.:..
plant   589 SSDRGPKCFG----TPLVTYINKEP--LSGTDIATSISGLLSPLRRV------HMSCVVNSGNEN 641

  Fly  1178 NHSGENSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEE 1242
            .|..:.|..|.|:.     |.|                |..||       ..|..||..|..:
plant   642 GHVPDESSRSILSR-----DTE----------------TEDND-------RELSLSLLRDYYS-- 676

  Fly  1243 GNAEDHDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFG 1307
                        ...||..|..|.:      |....|.||..:.|  .|:.|..||....|.:
plant   677 ------------FNLQPLESDSVVN------PGSVTKVLVKWNEK--EHEKYDSSYLNDLPKV-- 719

  Fly  1308 VPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLT 1372
                        ||
plant   720 ------------HK--------------------------------------------------- 721

  Fly  1373 CAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLS 1437
                                                              |:.||:|.:
plant   722 --------------------------------------------------NVLAKKTMQ------ 730

  Fly  1438 YTPTTKYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEK 1502
                                                            |.:.|..||.||.:||.
plant   731 ------------------------------------------------EGISLFSCLEAFLAEEP 747

  Fly  1503 L---EQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPY 1564
            |   :.|| |..||..:.|.|||.:||||.||:.|||||........|...:|:|...|.|
plant   748 LGPDDMWY-CPGCKEHRQANKKLDLWKLPDILVFHLKRFTYSRYFKNKIDTLVNFHIHDLD---- 807

  Fly  1565 LASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPT 1629
                                                    .||.||.|
plant   808 ----------------------------------------LSKYVKNE----------------- 815

  Fly  1630 GNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLN 1694
                                         ||  :.|              .|.|||:.:|.|.|.
plant   816 -----------------------------DG--QSY--------------LYELYAISNHYGGLG 835

  Fly  1695 GGHYISYAS--NATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYE 1744
            ||||.:||.  :.| .||.::||....:::.. |..||||:|||:|...|.|
plant   836 GGHYTAYAKLMDET-KWYNFDDSRVSAVNESE-IKTSAAYVLFYQRVKSDSE 885

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715
		EF-hand motif	198..222	CDD:320029
		EF-hand_7	227..290	CDD:463900
		UBP12	486..>1002	CDD:227847	167/568 (29%)
		Peptidase_C19	<1480..1737	CDD:470612	67/261 (26%)
UBP11	NP_174562.2	UBP12	20..879	CDD:227847	308/1409 (22%)