Protein Alignment Usp32 and UBP8

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_568411.4
• Gene: UBP8 / 832263
• AraportID: AT5G22030
• Length: 913
• Species: Arabidopsis thaliana
• Alignment Length: 1323
• Identity: 321/1323 - (24%)
• Similarity: 457/1323 - (34%)
• Gaps: 525/1323 - (39%)

  Fly   475 SASSGISAGRHCGPVR-PGPIDNSNLI-----TANPFRNVRTLTGEGGHLKRDTPLVQNHDFELV 533
            :||.|.|.|   ||:: ...|.||:::     ..:..:|     ||.|...     |...||.||
plant    54 TASCGSSYG---GPMKLINNIFNSDILFDLRREGDALQN-----GETGEAS-----VSGRDFALV 105

  Fly   534 PKSLW-KALNRWYGDNLPLPRQVIQPPNSDVE----------LELYPLNLRILLHQAQPSQTGVG 587
            ...:| :||            :.|:..:|.:|          ||...|..|:..|..:.::.||
plant   106 SSDMWLQAL------------KCIRSNDSFMEFFVFSASVHSLENEFLKKRLRYHDDKNTEKGV- 157

  Fly   588 GGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFL----- 647
                    .|..:||   :..|..|......||||.... ||::|..|....::.:...|
plant   158 --------KSFSAGG---VDRGDVYPVQLRLSVLQETNS-LAWSARLSFDKMIKNLNWILDNSVE 210

  Fly   648 -----CEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLT------ 701
                 |:...|.||.:|:|.:.  ....:..|.|....|:...:.:.::|||::...|:
plant   211 CFRRACKIFSLDSEQLRIWDIS--GQTTLFFESDVSNSKDCQQQADQEILLELQIYGLSDSIKLK 273

  Fly   702 -WPEELGSLATAQC-----GQGAGTPGDRRRLTRSSIMS-------VHAPGATGLHNLGNTCFMN 753
             ..:|.||  |.|.     |...||.   .|..||:.:|       ....|.|||.|||||||||
plant   274 ESKKEDGS--TQQTNGITNGMNGGTV---FRFGRSNSLSFLGKAGEAGTLGLTGLQNLGNTCFMN 333

  Fly   754 AALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCV 818
            ::||.|.:|..|..:|..|..: |:|..|.||.||::|:.:.:||:.:|.....:|||...:..:
plant   334 SSLQCLAHTPKLVDFFLGEYSK-EINLDNPLGMKGEIALAFGDLLRSLWAPGASTVAPRTFKAKL 397

  Fly   819 NKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQ 883
            .::||||:|..||||||||.:|||.||||||||..|||.|.||.:||||..||.|.|..|.|||.
plant   398 ARFAPQFSGFNQHDSQELLAFLLDGLHEDLNRVKNKPYVEAKDGDGRPDAEVADEYWRNHVARND 462

  Fly   884 SIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDG-SVPIKYGFRL 947
            |||:|:..||.||.:.|..|...||.||||..||||||..:....::.|:..|| |:||.....:
plant   463 SIIVDVCQGQYKSTLVCPICKKVSVMFDPFMYLSLPLPCTSMRTMDLTVMSADGSSLPIPLTVNV 527

  Fly   948 NSDCKYSHLKHKLSTMCSLP-PNLMLVCELWNSQIRQVLND--DEKLRTQSAKELYVYQLPEQSM 1009
            ....|:..|...|.|.|||| ...:||.|::|::|.:.|.:  |.....:...:|.||:|.:.:
plant   528 PKFGKFEDLHKALVTACSLPEEETLLVTEVYNNRIIRFLEEPTDSLTLIRDGDKLVVYRLKKDA- 591

  Fly  1010 RTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEA 1074
               :||.|.:::.|.|::...|.          .|..|.:.......|| ||       |||.
plant   592 ---NNSPLIVYMHQKLEEQFISG----------KSSPTWKAFGIPLVSR-LC-------DVEN-- 633

  Fly  1075 EVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKRECMPHSLFCFKESLI 1139
              |:||
plant   634 --GSDV----------------------------------------------------------- 637

  Fly  1140 LSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSR 1204
                 ||.::       |.:||.| :.||..|.::       ||..|...|:.
plant   638 -----ENLYL-------KLLSSFK-MPTEFFTENL-------ENPTEEEATDK------------ 670

  Fly  1205 NGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITT--SQPETSSGVYS 1267
                 .|....||..|::          |.||.|..|       ..||.:..  ...:..:.:.:
plant   671 -----TDTDGTTSVEDTN----------STDVKETTE-------SLPDPVLRLYLTDDRGNSIEA 713

  Fly  1268 RRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYCAVW---- 1328
            ....:.|....|.|..:.|                                         |
plant   714 EMLKEKPVNKSKRLNVLAR-----------------------------------------WPVKE 737

  Fly  1329 LQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCNNDY 1393
            |.|                         ||             ||
plant   738 LDV-------------------------YD-------------TC-------------------- 744

  Fly  1394 VLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWDPTA 1458
             |..:||.:  :.|.|..|:
plant   745 -LLSSLPEV--SKSGTKRPQ--------------------------------------------- 761

  Fly  1459 LHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL---EQWYHCSHCKGKKPATK 1520
                                       |.|.|..||.||.:||.|   :.|| |..||..:.|.|
plant   762 ---------------------------ESVSLFKCLEAFLTEEPLGPDDMWY-CPGCKEHRQAIK 798

  Fly  1521 KLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILRHKELLELKND 1585
            ||.:|:||.||::|||||:.......|.:..|.||.|:.|.:.|::               .||
plant   799 KLDLWRLPEILVIHLKRFSYSRFMKNKLEAYVDFPLDNLDLSSYIS---------------YKN- 847

  Fly  1586 AEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTKNAVRRQRLIST 1650
                                            |.|..
plant   848 --------------------------------GQTTY---------------------------- 852

  Fly  1651 SLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDS 1715
                                         ||.|||:.:|.|.:.||||.:|..:....||.::||
plant   853 -----------------------------RYMLYAISNHYGSMGGGHYTAYVHHGGDRWYDFDDS 888

  Fly  1716 SCREISQKPVIDPSAAYLLFYER 1738
            ...:|||:. |..||||:|||:|
plant   889 HVHQISQEK-IKTSAAYVLFYKR 910

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715
		EF-hand motif	198..222	CDD:320029
		EF-hand_7	227..290	CDD:463900
		UBP12	486..>1002	CDD:227847	186/565 (33%)
		Peptidase_C19	<1480..1737	CDD:470612	66/259 (25%)
UBP8	NP_568411.4	UBP12	168..910	CDD:227847	285/1170 (24%)