Protein Alignment Usp32 and USP9Y

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_004645.2
• Gene: USP9Y / 8287
• HGNCID: 12633
• Length: 2555
• Species: Homo sapiens
• Alignment Length: 1901
• Identity: 339/1901 - (17%)
• Similarity: 550/1901 - (28%)
• Gaps: 848/1901 - (44%)

  Fly   123 NLAPF-ESVSLFA----------------------QSERVNFEQF-----QDWIIKHRNATVLSK 159
            |||.: .|:.|:|                      ..||:||.:|     |.|:...:...:
Human   620 NLATYMNSIRLYAGDHEDYDPQTVRLGSRYSHVQEVQERLNFLRFLLKDGQLWLCAPQAKQI--- 681

  Fly   160 W-LLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIG-DLEKEFWRLKNT--------SQNGQ--- 211
            | .|::|.|.|........:|..|.|    :|.|:. |:.|:|:. .|.        ::||.
Human   682 WKCLAENAVYLCDREACFKWYSKLMG----DEPDLDPDINKDFFE-SNVLQLDPSLLTENGMKCF 741

  Fly   212 ----------------------------IDLQFLGPLISPPIPKNALAGLFNAFDENRDGHID-F 247
                                        |.|.:|..::            ..:.||..:..|| .
Human   742 ERFFKAVNCRERKLIAKRRSYMMDDLELIGLDYLWRVV------------IQSSDEIANRAIDLL 794

  Fly   248 KELCCGVSAACRGPGVERTRF---------CFK----------IFDVDRDGV-LSHDETLQMINV 292
            ||:...:     ||.::..:.         ||.          :||.|::.: .:..|.::|:.|
Human   795 KEIYTNL-----GPRLKANQVVIHEDFIQSCFDRLKASYDTLCVFDGDKNSINCARQEAIRMVRV 854

  Fly   293 LLLVAKE--NQ-ESQQYKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCD 354
            |.:: ||  |: :|..:|:
Human   855 LTVI-KEYINECDSDYHKE---------------------------------------------- 872

  Fly   355 LRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQY 419
             |::.|:....            :.||:...:         |.|.:..|...|   |.|
Human   873 -RMILPMSRAF------------RGKHLSLIV---------RFPNQGRQVDEL---DIW------ 906

  Fly   420 TQHTTHTCDYCKR-------------------------TASQRTAVDEALVCDESFNTHSLEQHD 459
             .||..|....:|                         :...|..:.:..:.|:|..|..|.| .
Human   907 -SHTNDTIGSVRRCIVNRIKANVAHKKIELFVGGELIDSEDDRKLIGQLNLKDKSLITAKLTQ-I 969

  Fly   460 SYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPL 524
            ::::.|...|:|.|.:|    |.|.|......||    ||...:....|               :
Human   970 NFNMPSSPDSSSDSSTA----SPGNHRNHYNDGP----NLEVESCLPGV---------------I 1011

  Fly   525 VQNHDFELVPK---SLWKALNRWYGDNLPLPRQVIQPPNSDVELELYPLNLRILLHQAQPSQTGV 586
            :..|     |:   .||:..:  .|.||.:      ||..|        ..|:|:....|.:|.|
Human  1012 MSVH-----PRYISFLWQVAD--LGSNLNM------PPLRD--------GARVLMKLMPPDRTAV 1055

  Fly   587 GGGTQLGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLC--- 648
               .:|    ..|...:..|..|          .|.||...|.:..:.|::..:.:|...|.
Human  1056 ---EKL----RAVCLDHAKLGEG----------KLSPPLDSLFFGPSASQVLYLTEVVYALLMPA 1103

  Fly   649 -EQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDN--DQLLLEIRNKDLTWPEELGSLA 710
             ..|...|.|   :.|..|.:|.:.|      :..:|||:|  ....:|.|........::..|.
Human  1104 GVPLTDGSSD---FQVHFLKSGGLPL------VLSMLIRNNFLPNTDMETRRGAYLNALKIAKLL 1159

  Fly   711 TAQCGQG-------------AGT---------PGDRRRLTRSSIMSVHAPGATGLHNLGNTCFMN 753
            ....|.|             .||         ..|:..:.:|::.|:..|.:..:  |.|...:
Human  1160 LTAIGYGHVRAVAEACQPVVDGTDPITQINQVTHDQAVVLQSALQSIPNPSSECV--LRNESIL- 1221

  Fly   754 AALQVLFNTQPLAQYFQREMHRF-----EVNAANKL---GTKGQLAMRYA--ELLKEVWTATTRS 808
                       |||....|..|:     .:.|..|:   ...|.|.:.::  |.:.:::..||..
Human  1222 -----------LAQEISNEASRYMPDICVIRAIQKIIWASACGALGLVFSPNEEITKIYQMTTNG 1275

  Fly   809 VAPLKL---RFCVNKYAPQ---FAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPD 867
            ...|::   :.|.......   ||         ||...||||.::
Human  1276 SNKLEVEDEQVCCEALEVMTLCFA---------LLPTALDALSKE-------------------- 1311

  Fly   868 KIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVR---FDPFSLLSL-------PLPV 922
                 :||       |:.||||.          |.|..::||   .:.|.|:..       ||
Human  1312 -----KAW-------QTFIIDLL----------LHCPSKTVRQLAQEQFFLMCTRCCMGHRPL-- 1352

  Fly   923 ENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSH-----LKHKLSTM----CSLP-PNLMLVCEL- 976
               ::|..|:..:.||...:.|       |||.     |:|.|:..    .::| ..::||.|:
Human  1353 ---LFFITLLFTILGSTAREKG-------KYSGDYFTLLRHLLNYAYNGNINIPNAEVLLVSEID 1407

  Fly   977 WNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDS 1041
            |..:||                        .:::....:|:...|.:|...:.:
Human  1408 WLKRIR------------------------DNVKNTGETGVEEPILEGHLGVTK----------- 1437

  Fly  1042 LSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQV 1106
              .|...|||..:                                  |:     :.....|.|.:
Human  1438 --ELLAFQTSEKK----------------------------------YH-----FGCEKGGANLI 1461

  Fly  1107 HELLPD---EAGKVSRCFGKRECMPHSLFCFKESLILSSSPEN---------TFMHGAAAQQKRV 1159
            .||:.|   .|.||...:.:...:|     .::::.:.|||..         ....|.....|::
Human  1462 KELIDDFIFPASKVYLQYLRSGELP-----AEQAIPVCSSPVTINAGFELLVALAIGCVRNLKQI 1521

  Fly  1160 SSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPLAD---LEPVSSRNGSG-------GEDCSY 1214
            ...        .:.|.|        |.:::|....|.:   |.||..|...|       |..| |
Human  1522 VDC--------LTEMYY--------MGTAITTCEALTEWEYLPPVGPRPPKGFVGLKNAGATC-Y 1569

  Fly  1215 RTS--------PNDSSGL----STGHTLGASLDVDE-QAEEGNAEDHDQ----PDQI-------- 1254
            ..|        |:..:.:    .||..|...:..|| |..|.|.:..|.    |.|.
Human  1570 MNSVIQQLYMIPSIRNSILAIEGTGSDLHDDMFGDEKQDSESNVDPRDDVFGYPHQFEDKPALSK 1634

  Fly  1255 TTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRP-----SLFGVPLLIPN 1314
            |..:.|.:.||.            ::|..:...:....   |.|:..|.     .|:|.|:.:
Human  1635 TEDRKEYNIGVL------------RHLQVIFGHLAASQ---LQYYVPRGFWKQFRLWGEPVNL-- 1682

  Fly  1315 SEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWS 1379
                  ::.:.|:.. .:.|:..|....:...|.|.....||..|        ||         .
Human  1683 ------REQHDALEF-FNSLVDSLDEALKALGHPAILSKVLGGSF--------AD---------Q 1723

  Fly  1380 SFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKY 1444
            ..|:||..|                                           |....|:|
Human  1724 KICQGCPHR-------------------------------------------YECEESFT----- 1740

  Fly  1445 FEDFTIAIDWDPTALHLR-YQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYH 1508
                |:.:|       :| :|:.|:.|            ||....||.....|          ||
Human  1741 ----TLNVD-------IRNHQNLLDSL------------EQYIKGDLLEGANA----------YH 1772

  Fly  1509 CSHCKGKKPATKKLQIWKLPPILIVHLKRFN------CVNGKWVKSQKVVHFPFDDFDPTPY--- 1564
            |..|..|....|:|.|.|||.:|.:.||||:      |.    :|......|| .:.|..||
Human  1773 CEKCDKKVDTVKRLLIKKLPRVLAIQLKRFDYDWERECA----IKFNDYFEFP-RELDMGPYTVA 1832

  Fly  1565 -LASVPQETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQT-GSTKATASPP 1627
             :|::.::.:....||:                           |.||:..|:| |.||
Human  1833 GVANLERDNVNSENELI---------------------------EQKEQSDNETAGGTK------ 1864

  Fly  1628 PTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGM 1692
                                                                 |||..|:.|||.
Human  1865 -----------------------------------------------------YRLVGVLVHSGQ 1876

  Fly  1693 LNGGHYISY------ASNATGSWYCYNDSSCREISQK----------------PVIDPSA----- 1730
            .:||||.||      ..:.|..||.::|....|....                .|.|...
Human  1877 ASGGHYYSYIIQRNGKDDQTDHWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMMKRMSY 1941

  Fly  1731 --------AYLLFYER 1738
                    ||:||||:
Human  1942 RRQKRWWNAYILFYEQ 1957

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	14/92 (15%)
		EF-hand motif	198..222	CDD:320029	8/62 (13%)
		EF-hand_7	227..290	CDD:463900	14/83 (17%)
		UBP12	486..>1002	CDD:227847	110/583 (19%)
		Peptidase_C19	<1480..1737	CDD:470612	63/302 (21%)
USP9Y	NP_004645.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..66
		Ubl1_cv_Nsp3_N-like	882..965	CDD:475130	16/113 (14%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	972..997		8/28 (29%)
		peptidase_C19C	1557..1960	CDD:239124	116/615 (19%)
		DUF3517	2098..2477	CDD:463438
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	2476..2555