Protein Alignment Usp32 and USP9X

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_001397677.1
• Gene: USP9X / 8239
• HGNCID: 12632
• Length: 2575
• Species: Homo sapiens
• Alignment Length: 2136
• Identity: 367/2136 - (17%)
• Similarity: 618/2136 - (28%)
• Gaps: 890/2136 - (41%)

  Fly    93 TQAEKTKFLWNLYCNDAGTFIIKS------------DYVRNVNLAPFESVSLFAQSERVNFE--- 142
            |..|..|...|...|||.:.||||            :.|:|:.:...:.:....|....|.:
Human   290 TDEELKKEAKNEAKNDALSMIIKSLKNLASRVPGQEETVKNLEIFRLKMILRLLQISSFNGKMNA 354

  Fly   143 ---------------------QFQDWIIKHRNATVLSKWLLSDNCVS--LTSELETPTFYQSLAG 184
                                 :.::|:...|    :::|:..:|.:|  |...|..|.:.:.|..
Human   355 LNEVNKVISSVSYYTHRHGNPEEEEWLTAER----MAEWIQQNNILSIVLRDSLHQPQYVEKLEK 415

  Fly   185 VTH--LEEK--DIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNA---LAGLFNAFDENRD 242
            :..  ::||  .:.||: ..|    .:|.|:.:          .|.||.   ||.|...|...:.
Human   416 ILRFVIKEKALTLQDLD-NIW----AAQAGKHE----------AIVKNVHDLLAKLAWDFSPEQL 465

  Fly   243 GHI--DFKELCCGVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQ 305
            .|:  .||......|...|...:|..|   ::.:.|:|||::|    :::|:|..:|..:.....
Human   466 DHLFDCFKASWTNASKKQREKLLELIR---RLAEDDKDGVMAH----KVLNLLWNLAHSDDVPVD 523

  Fly   306 YKDLTKQLVISDLLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCH 370
            ..||.....|. :|::...:.         ||...:...|..:..::.:.:.:.|.|..|.|:|.
Human   524 IMDLALSAHIK-ILDYSCSQD---------RDTQKIQWIDRFIEELRTNDKWVIPALKQIREICS 578

  Fly   371 IVFGLWPQ----------------------------------------CKHMENDIVRGWLR-RE 394
            : ||..||                                        ..:||:  :|.:.| .|
Human   579 L-FGEAPQNLSSSRFSQTQRSPHVFYRHDLINQLQHNHALVTLVAENLATYMES--MRLYARDHE 640

  Fly   395 ERRPYRV-------------------------GQFWYLITHDWWLSWMQYTQHTTHTCD--YCKR 432
            :..|..|                         ||.| |........|....::..:.||  .|.:
Human   641 DYDPQTVRLGSRYSHVQEVQERLNFLRFLLKDGQLW-LCAPQAKQIWKCLAENAVYLCDREACFK 704

  Fly   433 TASQRTAVDEALVCD---ESFNTHSLEQHDSYSLGSGTGS------------------------- 469
            ..|:....:..|..|   :.|.::.|:...|....:|...
Human   705 WYSKLMGDEPDLDPDINKDFFESNVLQLDPSLLTENGMKCFERFFKAVNCREGKLVAKRRAYMMD 769

  Fly   470 ---------------ASGSGSASSGISAGR----HCGP--------------------------- 488
                           .|....||..|...:    :.||
Human   770 DLELIGLDYLWRVVIQSNDDIASRAIDLLKEIYTNLGPRLQVNQVVIHEDFIQSCFDRLKASYDT 834

  Fly   489 --VRPGPIDNSNLITANPFRNVRTLT-------------------------GEGGHLKRDTPLVQ 526
              |..|..|:.|.......|.||.||                         ..|.||   :.:|:
Human   835 LCVLDGDKDSVNCARQEAVRMVRVLTVLREYINECDSDYHEERTILPMSRAFRGKHL---SFVVR 896

  Fly   527 --NHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDV---ELELY-------PLNLRILLHQA 579
              |...::....:|...|...|.   :.|.::....::|   ::||:       |.:.|.|:.|.
Human   897 FPNQGRQVDDLEVWSHTNDTIGS---VRRCILNRIKANVAHTKIELFVGGELIDPADDRKLIGQL 958

  Fly   580 ------------------QPSQTGVGGGTQLGSWGSTVSGGYGVLASGGGYAAI-----AVSSVL 621
                              .||.......:..||     .|.:|...|.|....:     .|...|
Human   959 NLKDKSLITAKLTQISSNMPSSPDSSSDSSTGS-----PGNHGNHYSDGPNPEVESCLPGVIMSL 1018

  Fly   622 QPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIR 686
            .|  ||:::                             ||.|..|.:.          |....:|
Human  1019 HP--RYISF-----------------------------LWQVADLGSS----------LNMPPLR 1042

  Fly   687 DNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCF 751
            |..::|:::...|.|..|:|.::..           |..:|..||:    :|      :|.:..|
Human  1043 DGARVLMKLMPPDSTTIEKLRAICL-----------DHAKLGESSL----SP------SLDSLFF 1086

  Fly   752 MNAALQVLFNTQ-----------PLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTAT 805
            ..:|.|||:.|:           |||.    :...|:.:.....|            |..|.:..
Human  1087 GPSASQVLYLTEVVYALLMPAGAPLAD----DSSDFQFHFLKSGG------------LPLVLSML 1135

  Fly   806 TRSVAPLKLRFCVNKYAP----QFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRP 866
            ||           |.:.|    :...|...::.::.:.||.|:.
Human  1136 TR-----------NNFLPNADMETRRGAYLNALKIAKLLLTAIG--------------------- 1168

  Fly   867 DKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVL 931
                                    ||.:::.......|.|.|.         |:...|.:..:..
Human  1169 ------------------------YGHVRAVAEACQPGVEGVN---------PMTQINQVTHDQA 1200

  Fly   932 VILLDG--SVPIKYGFRLNSDCKYSHLKHKLSTMCS-----LPPNLMLVCE----LWNS---QIR 982
            |:|...  |:|     ..:|:|...::..:|:...|     ..|::.::..    :|.|   .::
Human  1201 VVLQSALQSIP-----NPSSECMLRNVSVRLAQQISDEASRYMPDICVIRAIQKIIWASGCGSLQ 1260

  Fly   983 QVLNDDEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIE------QGLKDIQRSSALITSAQDS 1041
            .|.:.:|::..       :|:        ::|:|....:|      :.|:.:....|||.:|.|:
Human  1261 LVFSPNEEITK-------IYE--------KTNAGNEPDLEDEQVCCEALEVMTLCFALIPTALDA 1310

  Fly  1042 LSSLSTLQT-------SSHRASSRVL------------CNGH------------VSGLDVEGEAE 1075
            ||.....||       ..|..:.|.:            |.||            |.|......|:
Human  1311 LSKEKAWQTFIIDLLLHCHSKTVRQVAQEQFFLMCTRCCMGHRPLLFFITLLFTVLGSTARERAK 1375

  Fly  1076 VGTD---VSQCNSNSNYNPIVSTYSGNGSGDNQVHEL--LPDEAGKVSRCFGKRECMPHSLFCFK 1135
            ...|   :.:...|..||..::..:.....:|::..|  :.|:..:......:...:...|...|
Human  1376 HSGDYFTLLRHLLNYAYNSNINVPNAEVLLNNEIDWLKRIRDDVKRTGETGIEETILEGHLGVTK 1440

  Fly  1136 ESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTN------HSGENSME-------- 1186
            |.|...:| |..|..|.     ....|.|:....:......:|      .:||...|
Human  1441 ELLAFQTS-EKKFHIGC-----EKGGANLIKELIDDFIFPASNVYLQYMRNGELPAEQAIPVCGS 1499

  Fly  1187 --------------------------SSLTEPIPLAD-------------LEPVSSRNGSG---- 1208
                                      .||||...:..             |.||..|...|
Human  1500 PPTINAGFELLVALAVGCVRNLKQIVDSLTEMYYIGTAITTCEALTEWEYLPPVGPRPPKGFVGL 1564

  Fly  1209 ---GEDCSYRTS--------PNDSSGLSTGHTLGASLDVD-----EQAEEGNAEDHDQ----PDQ 1253
               |..| |..|        |:..:|:......|:.:|.|     :|..|.|.:..|.    |.|
Human  1565 KNAGATC-YMNSVIQQLYMIPSIRNGILAIEGTGSDVDDDMSGDEKQDNESNVDPRDDVFGYPQQ 1628

  Fly  1254 I--------TTSQPETSSGVYSRRSSQPPHKAGKYLVAV--HRKITRHDSYFLSYHKTRP----- 1303
            .        |..:.|.:.||.            ::|..:  |...:|     |.|:..|.
Human  1629 FEDKPALSKTEDRKEYNIGVL------------RHLQVIFGHLAASR-----LQYYVPRGFWKQF 1676

  Fly  1304 SLFGVPLLIPNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKA 1368
            .|:|.|:.:        ::.:.|:.. .:.|:..|....:...|.|.....||..|        |
Human  1677 RLWGEPVNL--------REQHDALEF-FNSLVDSLDEALKALGHPAMLSKVLGGSF--------A 1724

  Fly  1369 DGLTCAICPWSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYT 1433
            |         ...|:||..|                                           |.
Human  1725 D---------QKICQGCPHR-------------------------------------------YE 1737

  Fly  1434 ASLSYTPTTKYFEDFTIAIDWDPTALHLR-YQSTLERLWVDHETIAISRREQVEPVDLNHCLRAF 1497
            ...|:|         |:.:|       :| :|:.|:.|            ||....||.....|
Human  1738 CEESFT---------TLNVD-------IRNHQNLLDSL------------EQYVKGDLLEGANA- 1773

  Fly  1498 TSEEKLEQWYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPT 1562
                     |||..|..|....|:|.|.||||:|.:.||||                   |:|
Human  1774 ---------YHCEKCNKKVDTVKRLLIKKLPPVLAIQLKRF-------------------DYD-- 1808

  Fly  1563 PYLASVPQETILRHKELLELKNDAEMTMATNEVVSEL--DEIDAPSKEVK--EELPNQT-GSTKA 1622
                 ..:|..::..:..|...:.:|...|...|::|  |.::..|:.::  |:..::| ||||
Human  1809 -----WERECAIKFNDYFEFPRELDMEPYTVAGVAKLEGDNVNPESQLIQQSEQSESETAGSTK- 1867

  Fly  1623 TASPPPTGNILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVV 1687
                                                                      |||..|:
Human  1868 ----------------------------------------------------------YRLVGVL 1874

  Fly  1688 SHSGMLNGGHYISYASNATGS------WYCYNDSSCREISQK----------------PVIDPSA 1730
            .|||..:||||.||.....|.      ||.::|....|....                .|.|...
Human  1875 VHSGQASGGHYYSYIIQRNGGDGERNRWYKFDDGDVTECKMDDDEEMKNQCFGGEYMGEVFDHMM 1939

  Fly  1731 -------------AYLLFYERKG-LDYEP----YLPNIEGRTLPNTASVPLEVDET 1768
                         ||:|||||.. :|.:.    |:..:...|.|:...:|..::.:
Human  1940 KRMSYRRQKRWWNAYILFYERMDTIDQDDELIRYISELAITTRPHQIIMPSAIERS 1995

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	13/57 (23%)
		EF-hand motif	198..222	CDD:320029	3/23 (13%)
		EF-hand_7	227..290	CDD:463900	18/67 (27%)
		UBP12	486..>1002	CDD:227847	99/633 (16%)
		Peptidase_C19	<1480..1737	CDD:470612	62/296 (21%)
USP9X	NP_001397677.1	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..65
		Ubl1_cv_Nsp3_N-like	<905..969	CDD:475130	11/66 (17%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	972..1004		8/36 (22%)
		peptidase_C19C	1560..1963	CDD:239124	117/612 (19%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1597..1626		7/28 (25%)
		DUF3517	2102..2481	CDD:463438