Protein Alignment Usp32 and usp19

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_012816883.1
• Gene: usp19 / 780341
• XenbaseID: XB-GENE-5869969
• Length: 1478
• Species: Xenopus tropicalis
• Alignment Length: 1134
• Identity: 268/1134 - (23%)
• Similarity: 396/1134 - (34%)
• Gaps: 405/1134 - (35%)

  Fly   727 LTRSSIMS----------VHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAA 781
            :|.|::.|          |..||.|||.|||||||||:.:|.|.||:.|..|||......|:|..
 Frog   615 MTHSAVSSENVQEEEEKRVCPPGFTGLVNLGNTCFMNSVIQSLSNTRELRDYFQDRSFESEINCN 679

  Fly   782 NKLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHE 846
            |.|||.|:||:.:|.||:.:|..|..:..|.||:..|...|.||.|..|||:||.:.:|||.|||
 Frog   680 NPLGTGGRLAVSFAVLLRALWKGTHHAFQPSKLKAIVASKASQFTGYAQHDAQEFMAFLLDGLHE 744

  Fly   847 DLNRVMEKPYSELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFD 911
            ||||:..|||:|..||:||||:.||.|||.:|..||.|.::|||.||.|||:.|..|...|:.||
 Frog   745 DLNRIQNKPYTETVDSDGRPDEEVADEAWLRHKMRNDSFVVDLFQGQYKSKLVCPVCAKVSITFD 809

  Fly   912 PFSLLSLPLPVENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCEL 976
            ||..|.:|||.:.    :||.:         |.|     .:..|.|         |...::....
 Frog   810 PFLYLPVPLPQKQ----KVLTV---------YYF-----AREPHKK---------PIKFLVSISK 847

  Fly   977 WNSQIRQVLNDDEKLRTQSAKELYVYQLPEQSMRTRS-NSGLSMHIEQGLKDIQRSSALI--TSA 1038
            .||...:||....|                 |:|.:: |..|:..::...:.|.|.|..:  .|.
 Frog   848 ENSSAAEVLESISK-----------------SVRVKAENLQLAEVVKNRFQRIFRPSCSLDSVSP 895

  Fly  1039 QDSLSSLSTLQTSSHRASSRVLCNGHVSGLDVEGEAEV-GTDVSQCNSNSNYNPIVSTYSGNGSG 1102
            .|.|.....|        |:.|....|....|:...:| ...:::|.:...
 Frog   896 NDLLFCFEVL--------SKELSKERVVVFQVQQRPQVPSIPITKCAACQK-------------- 938

  Fly  1103 DNQVHELLPDEAGKVSRCFGKREC---------MPHSLFCFKESL---ILSSSPENTFMHGAAAQ 1155
             .|:.|  .::..:.:||:....|         ..|...|..|::   .|.|.||:...:...||
 Frog   939 -KQMSE--EEKLKRCTRCYRVGYCNQVCQKSHWPDHKTACRPENIGYPFLISVPESRLTYSRLAQ 1000

  Fly  1156 QKRVSSAKLLHTESNTSSMSYTNHS----------GENSMESSLTEPIPL----ADLEPVSS--- 1203
                    ||.        .|..:|          |..|.:.:   |.||    |||.|.|.
 Frog  1001 --------LLE--------GYARYSVNVFQPPFIPGRTSPDHA---PQPLSSEKADLPPQSDLPL 1046

  Fly  1204 RNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPETSSGVYSR 1268
            ...|..|:..:.:..:..:.|.....|.||.|        :...|.:|.....|...|.|| :|.
 Frog  1047 LGESSEEEKDFHSVQSSQTTLPPLTCLSASGD--------SGIVHGKPPGDRVSLYSTDSG-FSE 1102

  Fly  1269 RSSQPPHK--AGKYLVAVHRKITRHDSYFLSYHKT-----RPSLFGVPLLIPNSEGGTHKDLYCA 1326
            .|:....:  :|.....::.|..:.::....:.::     ..|.|.:.::.||::....:|
 Frog  1103 HSTDTVREMDSGGEKETIYEKSVKPEAAVAGFQQSESVNVHASAFYINVIDPNNKEMKLED---- 1163

  Fly  1327 VWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSS--------FCR 1383
                  :..|||..|       .||..:|                     .|.:        ...
 Frog  1164 ------KGESPLELT-------EDCSLAL---------------------VWKNNERAKEFVLVE 1194

  Fly  1384 GCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDF 1448
            ..|:.|..|        |.:|:                   ||.|...:|
 Frog  1195 SKELECEED--------PGSAS-------------------EAARAGLFT--------------- 1217

  Fly  1449 TIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL---EQWYHCS 1510
                                                     |:.||..||..|.|   |.|| |.
 Frog  1218 -----------------------------------------LDQCLNLFTKPEVLAPEEAWY-CP 1240

  Fly  1511 HCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKW-VKSQKVVHFPFDDFDPTPYLASVPQETIL 1574
            .|...:.|:|:|.:|:||.|||:.||||:.....| .|...:|.||..:.|.:.:..
 Frog  1241 KCNQHREASKQLMLWRLPNILIIQLKRFSFRTFIWRDKINDMVDFPVRNLDLSTFCI-------- 1297

  Fly  1575 RHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSKTK 1639
                                                                             
 Frog  1298 ----------------------------------------------------------------- 1297

  Fly  1640 NAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISYA-- 1702
                                |:.|| ||.          |.|.||||::|.|.:.||||.:||
 Frog  1298 --------------------GQKED-HQR----------PIYDLYAVINHYGGMIGGHYTAYARL 1331

  Fly  1703 ---SNATGS---WYCYNDSSCREISQKPVIDPSAAYLLFYERKGLDYE-PYLPNIEGRTLPNTAS 1760
               .|:..|   |..::||:...:.:..|: ...||:|||.|:....| |...:...|.:...||
 Frog  1332 PNEKNSQRSDVGWRLFDDSTVTTVDESQVV-TRYAYVLFYRRRNSPVERPMRGHPADRRVDAGAS 1395

  Fly  1761 VPL----------EVDETEGELKKLCSIS 1779
            ..:          :::..|.||.:...:|
 Frog  1396 ADVAGSQASLLWQQLEAEEDELHRRLDLS 1424

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715
		EF-hand motif	198..222	CDD:320029
		EF-hand_7	227..290	CDD:463900
		UBP12	486..>1002	CDD:227847	113/284 (40%)
		Peptidase_C19	<1480..1737	CDD:470612	60/268 (22%)
usp19	XP_012816883.1	p23_like	53..135	CDD:107220
		p23_CS_SGT1_like	435..515	CDD:107223
		USP19_linker	506..637	CDD:465191	4/21 (19%)
		UBP12	<637..1374	CDD:227847	254/1060 (24%)