Protein Alignment Usp32 and usp32

DIOPT Version :9

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_686236.6
• Gene: usp32 / 557981
• ZFINID: ZDB-GENE-100210-5
• Length: 1585
• Species: Danio rerio
• Alignment Length: 1922
• Identity: 689/1922 - (35%)
• Similarity: 975/1922 - (50%)
• Gaps: 486/1922 - (25%)

  Fly     1 MGTKESKHSNSVSYEDSVKRVSDVELRRLRDAFKKSAGVGRNFLSRNAFQQDVLCEGVPPKIVDM 65
            ||.|||: ...:||:::||||:||||:|||||||:::|:. .::::..|.::||.:|||||:.::
Zfish     1 MGAKESR-IGFLSYDEAVKRVTDVELKRLRDAFKRTSGLS-VYMTQQCFFREVLGDGVPPKVAEV 63

  Fly    66 LYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSD---YVRNVN-LAP 126
            :|.:.|||.:|:.||.|:.||||:|||...||.|::::|:.:|:|||:::.|   .:|:|: ..|
Zfish    64 IYNSFGGTSKGLHFNYLIVGLVLLTRGRDEEKAKYIFSLFASDSGTFVVREDVENMLRSVDGEVP 128

  Fly   127 FESVSLFAQSERVNFEQFQDWIIKHRNATVLSKWLLSDN-CVSLTSELETPTFYQSLAGVTHLEE 190
            ......|.:.|:||:|:|:.|::::::|...|:||||.. ||:||.:.:||||||:|||||||||
Zfish   129 PSLRKCFTEGEKVNYEKFKSWLLQNKDAFTFSRWLLSGGVCVTLTDDSDTPTFYQTLAGVTHLEE 193

  Fly   191 KDIGDLEKEFWRLKNTSQNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVS 255
            .||.||||.:|.||..|:.|:.||:...||:||||..:...|||:|||||||.||||||:.||:|
Zfish   194 SDIIDLEKRYWLLKAQSRTGRFDLETFVPLVSPPIHASLSEGLFHAFDENRDNHIDFKEISCGLS 258

  Fly   256 AACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQ-ESQQYKDLTKQLVISDLL 319
            |.||||..||.:||||:||||||||||.||..:|:..||.|.|:|: ::....|.:...::.|:|
Zfish   259 ACCRGPVAERQKFCFKVFDVDRDGVLSRDEIHEMVVALLEVWKDNRTDTLPEFDSSVSEIVEDIL 323

  Fly   320 EFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMEN 384
            :...           |.....||.||:.:|:|:.  .|....|:|:|::||||.||.|.....|.
Zfish   324 KMHD-----------TTKQGHLTLEDYQIWSVKS--ALANEFLNLLFQVCHIVLGLRPATPEEEG 375

  Fly   385 DIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTH-------TCDYCKRTASQRTAVDE 442
            .|:||||.||.|...:..|.|:||:..||..|..|.::...       :.......:||..|..|
Zfish   376 QIIRGWLERESRHGLQPSQNWFLISMQWWQQWKDYVRYDNKSIVMEQPSVQGSGMRSSQSAASVE 440

  Fly   443 ALV------------CDESFNTHSLEQHDSYSLGSGTGSASG-------SGSASSGISAGRH--- 485
            .::            .:|.|       .|:.|..|....::|       |.||:....|.:|
Zfish   441 PILNRIGLVVRSPSSPEEKF-------PDNISSSSEASESTGNSLTPHISSSATDVCFARQHNIS 498

  Fly   486 -----C------------GPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELV 533
                 |            ...|||.|||..|:|..|.: ..|||.|||.||:...|:...:||.|
Zfish   499 DNNNQCFSSANGHLPNSLAVQRPGAIDNQPLVTTEPMK-APTLTMEGGRLKKSHQLLSGREFETV 562

  Fly   534 PKSLWKALNRWYGDNLPLPRQVIQPP-NSDVELELYPLNLRILLHQAQPSQTGVGGGTQLGSWGS 597
            |:.:|:||..|||.||.|||.||:.. .:..||||:||.|..|  :.||:..    ..|...|
Zfish   563 PEPVWRALYHWYGANLSLPRPVIKSTVKAQPELELFPLYLLFL--RQQPATR----SPQSNIW-- 619

  Fly   598 TVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWH 662
             |:.|             .|.|...|.||.||||..|||:.|::.:.|:|.::||:|.||:|||
Zfish   620 -VNMG-------------NVPSPNAPLKRVLAYTGCFSRMCTIKDIHEYLSQRLRIKEEDMRLW- 669

  Fly   663 VPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRL 727
            :...:|...||:::...|:.|.|.|...|::|:||||::||||:..:|.:       :..||.::
Zfish   670 LYNSENYLTLLDDEDHTLESLKIPDEQHLVIEVRNKDMSWPEEMSFIANS-------SKIDRHKV 727

  Fly   728 TRSSIMSVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAM 792
            ....       |||||.|||||||||:::|.:.||:||.:||....|.:|:|..|.:|.:|.:|.
Zfish   728 PTEK-------GATGLSNLGNTCFMNSSIQCVSNTKPLTEYFISGRHLYELNRTNPIGMRGHMAK 785

  Fly   793 RYAELLKEVWTATTRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYS 857
            .|.:|::|:|:.|.::|||||||:.:.||||:|.|..|.||||||.:|||.||||||||.||||.
Zfish   786 CYGDLVQELWSGTQKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYV 850

  Fly   858 ELKDSNGRPDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPV 922
            |||||:||||..||.|||..|..||:||::|||:|||:|:|.|..|||.|.|||||:.||||||:
Zfish   851 ELKDSDGRPDHEVATEAWENHLRRNRSIVVDLFHGQLRSQVKCKTCGHISARFDPFNFLSLPLPM 915

  Fly   923 ENYIYFEVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLND 987
            ::.::.|:.||.||||.|::||.|||.|.||..||.:||.:|||.|..:|:.|:..|.|:....|
Zfish   916 DSSMHLEITVIKLDGSTPVRYGLRLNMDEKYMGLKKQLSELCSLKPEQILLAEVHTSNIKNFPLD 980

  Fly   988 DEKLRTQSAKELYVYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSS 1052
            ::|:|......|..:::|.....|                               |..|.:||..
Zfish   981 NQKVRHSVNGFLCAFEIPVPGSPT-------------------------------SVTSPVQTEK 1014

  Fly  1053 HRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKV 1117
                                                 .||.:..:||    |.:
Zfish  1015 -------------------------------------PPIANGTAGN----NHI----------- 1027

  Fly  1118 SRCFGKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGE 1182
                ||...:|
Zfish  1028 ----GKSGLIP------------------------------------------------------ 1034

  Fly  1183 NSMESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAED 1247
            |.|.|::   :|....:|:                   ::|:..||    ::.|.:....|
Zfish  1035 NGMPSTV---VPCTLEKPL-------------------ANGIPNGH----AVPVQDSPFIG---- 1069

  Fly  1248 HDQPDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLI 1312
                                            |::|:|||:.|.:.||||..|.||||||:||::
Zfish  1070 --------------------------------YIIAMHRKMMRTELYFLSSQKNRPSLFGMPLIV 1102

  Fly  1313 PNSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICP 1377
            |.:...:.||||.|||:|||||.|||| ..|.:|||.||||||||.:|||||.|:.||.:||.||
Zfish  1103 PCTVHTSKKDLYDAVWIQVSRLASPLP-PQEASNHAQDCDDSLGYQYPFTLRVVQKDGNSCAWCP 1166

  Fly  1378 WSSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTT 1442
            |..|||||.:.||.|..                         ||.|             :|
Zfish  1167 WYRFCRGCTVDCNEDRA-------------------------SLGN-------------AY---- 1189

  Fly  1443 KYFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL--EQ 1505
                   ||:||||||||||||::.||:..:|.::..|||.|.||:.|:.||:||||||:|  ::
Zfish  1190 -------IAVDWDPTALHLRYQTSQERIVEEHPSVEQSRRAQAEPISLDSCLKAFTSEEELGEDE 1247

  Fly  1506 WYHCSHCKGKKPATKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQ 1570
            .|:||.||..:.|||||.:|:|||:||||||||..|||:|:||||:|.||.:.|||:.:||  |:
Zfish  1248 LYYCSKCKTHRLATKKLDLWRLPPVLIVHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFLA--PR 1310

  Fly  1571 ETILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTG----- 1630
            |.......|..|:::.        :|.||..::.....|      .:.||...||||.:|
Zfish  1311 EVSQSLGSLQSLQSEC--------LVEELQRLERGDSSV------SSISTTTLASPPISGRATPC 1361

  Fly  1631 -----------NILRQSKTKNAVRRQ------------RLIS---------------TSLTKTPI 1657
                       |....|..:|..|:|            ||.:               :...::.:
Zfish  1362 SIRRVGSPLSRNSSPGSSPRNTGRKQGRLRLPQLGSRHRLCNSKENLEACSTNKESESEAEQSAV 1426

  Fly  1658 VDGEFEDYHQHRLKPDVDQFD--------------------------------------PR---- 1680
            .|||       .|.||.:..|                                      |.
Zfish  1427 TDGE-------NLTPDANTQDTWSLDASSNDCDVIMVNGLGQDSSQSNGHSEVSADLDTPHQRND 1484

  Fly  1681 ------YRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERK 1739
                  |.|||:..|||::.||||::||.|....|||||||||:|: ....||..:||:||||::
Zfish  1485 ICLESLYNLYAISCHSGIMGGGHYVTYAKNPNEKWYCYNDSSCKEV-HSDEIDTDSAYILFYEQQ 1548

  Fly  1740 GLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLC 1776
            |:||..:||..||:.:.:|.|:.   ::.|.:.||.|
Zfish  1549 GVDYSQFLPKTEGKKMADTTSMD---EDFESDYKKYC 1582

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	FRQ1	186..317	CDD:227455	73/131 (56%)
		UBP12	486..>1002	CDD:227847	236/528 (45%)
		Peptidase_C19	<1480..1737	CDD:351799	114/349 (33%)
usp32	XP_686236.6	EF-hand_7	236..293	CDD:290234	41/56 (73%)
		EFh	237..293	CDD:238008	40/55 (73%)
		EF-hand_7	270..342	CDD:290234	30/82 (37%)
		EFh	270..338	CDD:298682	28/78 (36%)
		DUSP	<519..586	CDD:283896	34/67 (51%)
		UBP12	521..1546	CDD:227847	462/1339 (35%)
		Ubiquitin_3	623..710	CDD:291502	37/100 (37%)
		Peptidase_C19	733..>918	CDD:271592	110/184 (60%)
		Peptidase_C19	<1220..1546	CDD:271592	114/349 (33%)

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	631	1.000	Domainoid score	I168
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H13066
Inparanoid	1	1.050	1097	1.000	Inparanoid score	I230
OMA	1	1.010	-	-		QHG52451
OrthoDB	1	1.010	-	-		D1283205at2759
OrthoFinder	1	1.000	-	-		FOG0007271
OrthoInspector	1	1.000	-	-		oto39905
orthoMCL	1	0.900	-	-		OOG6_104647
Panther	1	1.100	-	-	LDO	PTHR21646
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4685
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	12	11.980