Protein Alignment Usp32 and Usp32

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_038941969.1
• Gene: Usp32 / 303394
• RGDID: 1306657
• Length: 1683
• Species: Rattus norvegicus
• Alignment Length: 1925
• Identity: 680/1925 - (35%)
• Similarity: 979/1925 - (50%)
• Gaps: 498/1925 - (25%)

  Fly    21 VSDVELRRLRDAFKKSAGVGRNFLSRNAFQQDVLCEGVPPKIVDMLYAACGGTQRGISFNDLLCG 85
            |:||||:||:||||::.|:. .::|::.|.::||.:|||||:.:::|.:.|||.:|:.||:|:.|
  Rat    85 VTDVELKRLKDAFKRTCGLS-YYMSQHCFIREVLGDGVPPKVAEVIYCSFGGTSKGLHFNNLIVG 148

  Fly    86 LVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNL----APFESVSLFAQSERVNFEQFQD 146
            |||:|||...||.|::::|:.:::|:::::.:..|.:::    .|......|::.|:||:|:|::
  Rat   149 LVLLTRGKDEEKAKYIFSLFSSESGSYVVREEMERMLHVVDGKVPDTLRKCFSEGEKVNYEKFRN 213

  Fly   147 WIIKHRNATVLSKWLLSDNC-VSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQNG 210
            |::.:::|...|:||||... |:||.:.:||||||:|||||||||.||.||||.:|.||..|:.|
  Rat   214 WLLLNKDAFTFSRWLLSGGVYVTLTDDSDTPTFYQTLAGVTHLEESDIIDLEKRYWLLKAQSRTG 278

  Fly   211 QIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIFDV 275
            :.||:..|||:||||..:...|||||||||||.||||||:.||:||.||||..||.:||||:||:
  Rat   279 RFDLETFGPLVSPPIRPSLSEGLFNAFDENRDNHIDFKEISCGLSACCRGPLAERQKFCFKVFDI 343

  Fly   276 DRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTK-QLVISDLLEFGQRRSPDGTPSKLTRDNV 339
            |||||||..|...|:..||.|.|:|:..    |:.: .:.:||::|   |.......:|:..
  Rat   344 DRDGVLSRVELKDMVVALLEVWKDNRTD----DIPELHMDLSDIVE---RILNAHDTTKMGH--- 398

  Fly   340 SLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHMENDIVRGWLRREERRPYRVGQF 404
             ||.||:.:|:|:  ..|....|:|:|::||||.||.|.....|..|:||||.||.|...:.|..
  Rat   399 -LTLEDYQIWSVK--NVLANEFLNLLFQVCHIVLGLRPATPEEEGQIIRGWLERESRYGLQPGHN 460

  Fly   405 WYLITHDWWLSWMQYTQH----------------------TTHTCDYCKRTASQRTAVDEALVCD 447
            |::|:..||..|.:|.::                      |.|..::.:...|...:.
  Rat   461 WFIISMQWWQQWKEYVKYDANPVVIEPSSVLNGGKFSFGTTAHPIEHGEDRISNNLSY------- 518

  Fly   448 ESFNTHSLEQHDSYSLGSGTGSASGSG---SASSG-------------------ISAG----RHC 486
              .||...:..|:.|..|....::|||   ||:.|                   :.|.    .|.
  Rat   519 --MNTTEEKYSDNISSASEASESTGSGFLYSATPGADMCFARQHNTSDNNNQCLLGANGNILLHL 581

  Fly   487 GPVRPGPIDNSNLITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPL 551
            .|.:||.|||..|:|..|.: ..:||.|||.|||...|:...|:|:||:.:|:||..|||.||.|
  Rat   582 NPQKPGAIDNQPLVTQEPVK-ATSLTLEGGRLKRTPQLIHGRDYEMVPEPVWRALYHWYGSNLAL 645

  Fly   552 PRQVIQPPNSDV-ELELYPLNLRILLHQ----AQPSQTGVG-GGTQLGSWGSTVSGGYGVLASGG 610
            ||.||:...:|: ||||:|..|..|..|    .|.|...|. |...|..:...:..|
  Rat   646 PRPVIKNSKTDIPELELFPRYLLFLRQQPATRTQQSNIWVNMGMMSLRMFPQHLPRG-------- 702

  Fly   611 GYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAILLEE 675
                 .|.|...|.||.||||..|||:.|::::.|:|.::||:|.||:||| :...:|...||::
  Rat   703 -----NVPSPNAPLKRVLAYTGCFSRMQTIKEIHEYLSQRLRIKEEDMRLW-LYNSENYLTLLDD 761

  Fly   676 DAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGA 740
            :...|:.|.|:|...|::|:||||::||||:..:|.:       :..||.::....       ||
  Rat   762 EDHRLEYLKIQDEQHLVIEVRNKDMSWPEEMSFIANS-------SKIDRHKVPTEK-------GA 812

  Fly   741 TGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTAT 805
            |||.|||||||||:::|.:.|||||.|||....|.:|:|..|.:|.||.:|..|.:|::|:|:.|
  Rat   813 TGLSNLGNTCFMNSSIQCVSNTQPLTQYFISGRHLYELNRTNPIGMKGHMAKCYGDLVQELWSGT 877

  Fly   806 TRSVAPLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIV 870
            .::|||||||:.:.||||:|.|..|.||||||.:|||.||||||||.||||.|||||:||||..|
  Rat   878 QKNVAPLKLRWTIAKYAPRFNGFQQQDSQELLAFLLDGLHEDLNRVHEKPYVELKDSDGRPDWEV 942

  Fly   871 AAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILL 935
            |||||..|..||:||::|||:|||:|:|.|..|||.|||||||:.||||||:::|::.|:.||.|
  Rat   943 AAEAWDNHLRRNRSIVVDLFHGQLRSQVKCKTCGHISVRFDPFNFLSLPLPMDSYMHLEITVIKL 1007

  Fly   936 DGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRTQSAKELY 1000
            ||:.||:||.|||.|.||:.||.:||.:|.|....:|..|:.:|.|:....|::|:|...:..|.
  Rat  1008 DGTTPIRYGLRLNMDEKYTGLKKQLSDLCGLKSEQILFAEVHSSNIKNFPQDNQKVRLSVSGFLC 1072

  Fly  1001 VYQLPEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCNGHV 1065
            .:::|..:                               ..:|:.|.:||
  Rat  1073 AFEIPIPA-------------------------------SPVSACSPIQT--------------- 1091

  Fly  1066 SGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKRECMPHS 1130
                            .|:|:.:.|.:. |.:.||.                         :|..
  Rat  1092 ----------------DCSSSPSTNGLF-TVTTNGD-------------------------LPRP 1114

  Fly  1131 LFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPIPL 1195
            :|.                                                .|.|.:::   :|
  Rat  1115 IFI------------------------------------------------PNGMPNTV---VP- 1127

  Fly  1196 ADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQPE 1260
                            |.  |..|.::|:..||.                  ...||...|.
  Rat  1128 ----------------CG--TEKNATNGIVNGHM------------------PPLPDDPFTG--- 1153

  Fly  1261 TSSGVYSRRSSQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKDLYC 1325
                               |::|||||:.|.:.||||..|.||||||:||::|.:.....||||.
  Rat  1154 -------------------YIIAVHRKMMRTELYFLSSQKNRPSLFGMPLIVPCTVHTRKKDLYD 1199

  Fly  1326 AVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEIRCN 1390
            |||:|||||.|||| ..|.:|||.|||||:||.:|||||.|:.||.:||.|||..|||||:|.|.
  Rat  1200 AVWIQVSRLASPLP-PQEASNHAQDCDDSMGYQYPFTLRVVQKDGNSCAWCPWYRFCRGCKIDCG 1263

  Fly  1391 NDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAIDWD 1455
            .|....|     ||.                                            ||:|||
  Rat  1264 EDRAFIG-----NAC--------------------------------------------IAVDWD 1279

  Fly  1456 PTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL--EQWYHCSHCKGKKPA 1518
            |||||||||::.||:..:||::..|||.|.||::|:.|||||||||:|  .:.|:||.||....|
  Rat  1280 PTALHLRYQTSQERVVEEHESVEQSRRAQAEPINLDSCLRAFTSEEELGENEMYYCSKCKTHCLA 1344

  Fly  1519 TKKLQIWKLPPILIVHLKRFNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQE-TILRHKELLEL 1582
            ||||.:|:||||||:|||||..|||:|:||||:|.||.:.|||:.:|  ||:: |:.:||.|...
  Rat  1345 TKKLDLWRLPPILIIHLKRFQFVNGRWIKSQKIVKFPRESFDPSAFL--VPRDPTLCQHKPLTPQ 1407

  Fly  1583 KNDAEMTMATNEVVSELDEIDAPSKEVKE-ELPNQTG-------------STKATASPPPTGNIL 1633
            .:|             :.|:..|:.:||: ::.|..|             |...|:||..:.:..
  Rat  1408 GDD-------------VSELPIPAGDVKKVDVQNPAGEEDVLLSKSPSSLSANITSSPKGSPSSS 1459

  Fly  1634 RQ---------------------------------SKTKNAVRRQRL-------------ISTSL 1652
            |:                                 ||.|.:..::.|             ::.:|
  Rat  1460 RKSGASCPSSKNSSPNSSPRTLGRNKGRLRLPQIGSKNKLSSSKENLDASRENGAGQICELADTL 1524

  Fly  1653 TK------------TPI----------------------------------VDGEFEDYHQHRLK 1671
            .:            ||:                                  .|.:.||.|.
  Rat  1525 NRGHVLGGSQPELVTPLDHEITLANGFLYEHETCGNGYSNGQLGNHSEEDSTDDQREDTHS---- 1585

  Fly  1672 PDVDQFDPRYRLYAVVSHSGMLNGGHYISYASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFY 1736
                  .|.|.|||:..|||:|.||||::||.|....|||||||||:|: ....||..:||:|||
  Rat  1586 ------KPIYNLYAISCHSGILGGGHYVTYAKNPNCKWYCYNDSSCKEL-HPDEIDTDSAYILFY 1643

  Fly  1737 ERKGLDYEPYLPNIEGRTLPNTASVPLEVDETEGELKKLC 1776
            |::|:|...:||.|:|:.:.:|:|:.   ::.|.:.||.|
  Rat  1644 EQQGIDCAQFLPKIDGKKMADTSSMD---EDFESDYKKYC 1680

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	32/52 (62%)
		EF-hand motif	198..222	CDD:320029	11/23 (48%)
		EF-hand_7	227..290	CDD:463900	41/62 (66%)
		UBP12	486..>1002	CDD:227847	240/521 (46%)
		Peptidase_C19	<1480..1737	CDD:470612	115/365 (32%)
Usp32	XP_038941969.1	EFh	302..358	CDD:238008	36/55 (65%)
		EF-hand_7	335..407	CDD:463900	24/72 (33%)
		UBP12	583..1395	CDD:227847	278/817 (34%)
		UCH	<1310..1643	CDD:425685	152/335 (45%)