Protein Alignment Usp32 and USP24

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: XP_016856320.1
• Gene: USP24 / 23358
• HGNCID: 12623
• Length: 2640
• Species: Homo sapiens
• Alignment Length: 2162
• Identity: 376/2162 - (17%)
• Similarity: 613/2162 - (28%)
• Gaps: 921/2162 - (42%)

  Fly    17 SVKRVSDVELRRLRDAFKKSAGVGRNFLSRNAFQQDVLCEGVPPKIVDMLYAACGGTQRGISFND 81
            |.|..|..|:.:|.:....|..|      :||...|.|.:.:....|  |..|..|......:.|
Human   401 SAKMNSLKEVTKLIEDSTLSKSV------KNAIDTDRLLDWLVENSV--LSIALEGNIDQAQYCD 457

  Fly    82 LLCGLV--LITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAPFESVSLFAQSERVNFEQF 144
            .:.|::  |.::.:..|.|| :|.:....:.|.|              |::.....:..|.|...
Human   458 RIKGIIELLGSKLSLDELTK-IWKIQSGQSSTVI--------------ENIHTIIAAAAVKFNSD 507

  Fly   145 QDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEEKDIGDLEKEFWRLKNTSQN 209
            |   :.|....:...|           |.|:....|.|..:       ||.:.:|. |.:.||
Human   508 Q---LNHLFVLIQKSW-----------ETESDRVRQKLLSL-------IGRIGREA-RFETTS-- 548

  Fly   210 GQIDLQFLGPLIS-PPIPKNALAGLFNAFDENRDGHIDFKELCCGVSAACRGPGVERTRFCFKIF 273
            |:: |..|..|.. |.:|.:.:.   .|.:|    |:........|..|     ::|:.....|.
Human   549 GKV-LDVLWELAHLPTLPSSLIQ---QALEE----HLTILSDAYAVKEA-----IKRSYIIKCIE 600

  Fly   274 DVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLV-ISDLLEFGQRRSPDGTPS----- 332
            |:.|.|..|..|            |..::..:...||...| :....|..||.|....|.
Human   601 DIKRPGEWSGLE------------KNKKDGFKQGGLTGDYVSLPGYTETKQRSSQLNNPQFVWVV 653

  Fly   333 -------KLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHM-------- 382
                   ::||..:..|.:       :.|..::|. |...||:..:|.|....|..:
Human   654 PALRQLHEITRSFIKQTYQ-------KQDKSIIQD-LKKNFEIVKLVTGSLIACHRLAAAVAGPG 710

  Fly   383 ------------------------------ENDIVRGWLRREERRPYRV-GQFWYLITHDWWLSW 416
                                          |..:..||.|.:|.....| ||....:..:....|
Human   711 GLSGSTLVDGRYTYREYLEAHLKFLAFFLQEATLYLGWNRAKEIWECLVTGQDVCELDREMCFEW 775

  Fly   417 MQYTQH------------------------------------TTHTCDY-CKRTASQ-------- 436
            ....||                                    ..:.||: .||..:|
Human   776 FTKGQHDLESDVQQQLFKEKILKLESYEITMNGFNLFKTFFENVNLCDHRLKRQGAQLYVEKLEL 840

  Fly   437 ----------RTAVDEALVCD-------------------ESFNTHSLEQHDSYSLGSGTGSASG 472
                      ..:.||.:..:                   :|.:.|.....|.|:......||.|
Human   841 IGMDFIWKIAMESPDEEIANEAIQLIINYSYINLNPRLKKDSVSLHKKFIADCYTRLEAASSALG 905

  Fly   473 SGSASSGISAGRH--CGPVRP-------GPIDNSNL--------------ITANPFRNV-RTLTG 513
            ..:.:..::....  .....|       .|..::.|              ||...|.:| ||:..
Human   906 GPTLTHAVTRATKMLTATAMPTVATSVQSPYRSTKLVIIERLLLLAERYVITIEDFYSVPRTILP 970

  Fly   514 EG----GHL---------KRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQPPNSDVEL 565
            .|    |||         .:||..|:.|..|.:....||           :.:|:..|.:
Human   971 HGASFHGHLLTLNVTYESTKDTFTVEAHSNETIGSVRWK-----------IAKQLCSPVD----- 1019

  Fly   566 ELYPLNLRI--------------LLHQ----------AQPSQTGVGGGTQLGSWGSTVSGGYGVL 606
                 |::|              ||||          .:.|.:|...|:...|..|:.|...||.
Human  1020 -----NIQIFTNDSLLTVNKDQKLLHQLGFSDEQILTVKTSGSGTPSGSSADSSTSSSSSSSGVF 1079

  Fly   607 ASGGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFLCEQLRLKSEDIRLWHVPQLDNGAI 671
            :|         |..::..|......     :|.|..|.:.|.:...|:...|.| .|.:|    :
Human  1080 SS---------SYAMEQEKSLPGVV-----MALVCNVFDMLYQLANLEEPRITL-RVRKL----L 1125

  Fly   672 LLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELG---SLATAQCGQGAGTPGDRRRLTRSSIM 733
            ||......::|.|    |||            :.||   :|.:....|.:.:|.       .|..
Human  1126 LLIPTDPAIQEAL----DQL------------DSLGRKKTLLSESSSQSSKSPS-------LSSK 1167

  Fly   734 SVHAPGATGLHNLGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELL 798
            ..|.|.|:.:            |:.||.:........|.::..||.::..:.|...
Human  1168 QQHQPSASSI------------LESLFRSFAPGMSTFRVLYNLEVLSSKLMPTADD--------- 1211

  Fly   799 KEVWTATTRSVAPLKLRFCVNKYAPQF--AGG-------GQHDS-----------------QELL 837
                     .:|    |.|...:...|  |||       .|.||                 .:|.
Human  1212 ---------DMA----RSCAKSFCENFLKAGGLSLVVNVMQRDSIPSEVDYETRQGVYSICLQLA 1263

  Fly   838 EWLL------DALHEDLNR-----VMEKPYSELKDSN-------GRPDKIVAAEAWSQHHARNQS 884
            .:||      ..|.|||.:     :..:|:..:....       |.|:|    .::.|....::|
Human  1264 RFLLVGQTMPTLLDEDLTKDGIEALSSRPFRNVSRQTSRQMSLCGTPEK----SSYRQLSVSDRS 1324

  Fly   885 -------------IIIDLFYGQLKSKVSC---LGCGHESVRFDPFSLLSLPLPVENYIYFEVLVI 933
                         .|..:......|.|:|   |.....:.|.|
Human  1325 SIRVEEIIPAARVAIQTMEVSDFTSTVACFMRLSWAAAAGRLD---------------------- 1367

  Fly   934 LLDGSVPIKYGFRLNSDCKYSHLKHKLSTM---CSLPP--------------------------- 968
            |:..|.|||   ..||.|. :.::::||:.   ||...
Human  1368 LVGSSQPIK---ESNSLCP-AGIRNRLSSSGSNCSSGSEGEPVALHAGICVRQQSVSTKDSLIAG 1428

  Fly   969 ---NLMLVC-ELWNSQIRQVLN---------------DDEKLRTQSAKELYVYQLPEQSMRTRSN 1014
               :|::.| :|.:.|:....|               ...::|..:..:||          |.|.
Human  1429 EALSLLVTCLQLRSQQLASFYNLPCVADFIIDILLGSPSAEIRRVACDQLY----------TLSQ 1483

  Fly  1015 SGLSMHIEQGLKDIQRSS----ALITSAQ-----------------------------DSLSSLS 1046
            :..|.|     .|:|:.:    .:|.:||                             ..|..|:
Human  1484 TDTSAH-----PDVQKPNQFLLGVILTAQLPLWSPTSIMRGVNQRLLSQCMEYFDLRCQLLDDLT 1543

  Fly  1047 TLQTSSHRASSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLP 1111
            |.:....|.|...:....::.||                  |:.|            |:..|...
Human  1544 TSEMEQLRISPATMLEDEITWLD------------------NFEP------------NRTAECET 1578

  Fly  1112 DEAGKV-------------SRCFGKRECMPHSL-------FCFKESLIL--SSSPENTFMHGAAA 1154
            .||..:             |.|..::|.:..||       |.|:.|.|:  |.||..:   .|.:
Human  1579 SEADNILLAGHLRLIKTLLSLCGAEKEMLGSSLIKPLLDDFLFRASRIILNSHSPAGS---AAIS 1640

  Fly  1155 QQ----------KRVSSAKLL-----HTESNTSSMSYTNHSGENSMESSLTEPIPLADLEPVSSR 1204
            ||          .|:::.::|     .:.||...:.....|..:..:.:||:....  |.||.||
Human  1641 QQDFHPKCSTANSRLAAYEVLVMLADSSPSNLQIIIKELLSMHHQPDPALTKEFDY--LPPVDSR 1703

  Fly  1205 NGS-------GGEDCSYRTSPNDSSGLSTGHTLGAS-LDVDEQAEEGNAEDHDQPDQITTSQPET 1261
            :.|       ||..| |..:......:..|  |..| |.||        :|.|.||.....|.::
Human  1704 SSSGFVGLRNGGATC-YMNAVFQQLYMQPG--LPESLLSVD--------DDTDNPDDSVFYQVQS 1757

  Fly  1262 SSG--VYSRRSSQPPHKAGKYLVAVHRKI---TRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHK 1321
            ..|  :.|:.....|....|.....::::   .:.|:|
Human  1758 LFGHLMESKLQYYVPENFWKIFKMWNKELYVREQQDAY--------------------------- 1795

  Fly  1322 DLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYD--FPFTLRAVKADGLTCAICPWSSFCRG 1384
            :.:.::..|:...|.                 .:|.|  |..|.:.:.:|...|..||
Human  1796 EFFTSLIDQMDEYLK-----------------KMGRDQIFKNTFQGIYSDQKICKDCP------- 1836

  Fly  1385 CEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFT 1449
                  :.|..:.|...:|...:            |..:||
Human  1837 ------HRYEREEAFMALNLGVT------------SCQSLE------------------------ 1859

  Fly  1450 IAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLE--QWYHCSHC 1512
            |::|                                          .|...|.||  ..|:|..|
Human  1860 ISLD------------------------------------------QFVRGEVLEGSNAYYCEKC 1882

  Fly  1513 KGKKPATKKLQIWKLPPILIVHLKR--FNCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQETILR 1575
            |.|:...|:..|..||.:|::||.|  |:..:|:.:|..:.:.||: ..:..||..|
Human  1883 KEKRITVKRTCIKSLPSVLVIHLMRFGFDWESGRSIKYDEQIRFPW-MLNMEPYTVS-------- 1938

  Fly  1576 HKELLELKNDAEMTMATNEVVSELDE----ID-----APSKEVKEELPNQTGSTKATASPPPTGN 1631
                         .||..:..||:.|    :|     :|.|:|
Human  1939 -------------GMARQDSSSEVGENGRSVDQGGGGSPRKKV---------------------- 1968

  Fly  1632 ILRQSKTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGG 1696
                               :||:                         .|.|..|:.|||..:.|
Human  1969 -------------------ALTE-------------------------NYELVGVIVHSGQAHAG 1989

  Fly  1697 HYISYASN----ATGSWYCYNDSSCREIS---------------QKPVIDPS-----------AA 1731
            ||.|:..:    ..|.||.:||:...|..               :..|.|.:           .|
Human  1990 HYYSFIKDRRGCGKGKWYKFNDTVIEEFDLNDETLEYECFGGEYRPKVYDQTNPYTDVRRRYWNA 2054

  Fly  1732 YLLFYERKGLDYEPYLP 1748
            |:|||:|......|.||
Human  2055 YMLFYQRVSDQNSPVLP 2071

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715	13/53 (25%)
		EF-hand motif	198..222	CDD:320029	8/23 (35%)
		EF-hand_7	227..290	CDD:463900	12/62 (19%)
		UBP12	486..>1002	CDD:227847	121/686 (18%)
		Peptidase_C19	<1480..1737	CDD:470612	58/299 (19%)
USP24	XP_016856320.1	UBA_UBP24	6..42	CDD:270472
		Ubl_UBP24	975..1053	CDD:340585	18/98 (18%)
		peptidase_C19C	1707..2064	CDD:239124	99/590 (17%)