DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Usp32 and cyk-3

DIOPT Version :9

Sequence 1:	NP_001262074.2	Gene:	Usp32 / 40169	FlyBaseID:	FBgn0036913	Length:	1779	Species:	Drosophila melanogaster
Sequence 2:	NP_001022992.1	Gene:	cyk-3 / 175853	WormBaseID:	WBGene00000874	Length:	1178	Species:	Caenorhabditis elegans

Alignment Length:	1793	Identity:	412/1793 - (22%)
Similarity:	608/1793 - (33%)	Gaps:	672/1793 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MGTKESKHSNS----VSYEDSVKRVSDVELRRLRDAFKKSAGVGRNFLSRNAFQQDVLCEGVPPK 61
            ||...:..||:    :|.||:...:||.|.||:|.||::        ........|..|
Worm     1 MGNTLTGRSNAIAPIISAEDAKTYISDEEYRRIRQAFQR--------FKNGCINYDEFC------ 51

  Fly    62 IVDMLYAACGGTQRGISFNDLLCGLVLITRGTQAEKTKFLWNLYCNDAGTFIIKSDYVRNVNLAP 126
                 |...||.|                  ...||.:.|::.:.:.|.|....:.....|.|..
Worm    52 -----YHVLGGAQ------------------IPEEKRRLLFSFFSHGAETISFDNLLSSLVGLCR 93

  Fly   127 FESVSLFAQSERV-NFEQFQDWIIKHRNATVLSKWLLSDNCVSLTSELETPTFYQSLAGVTHLEE 190
            .|.|    ||..: .:.:|..|.:.....|:    .|:|:.:|         ||:.::.||||..
Worm    94 VEEV----QSRFIEEYHEFASWGLSPPKLTI----PLNDSYIS---------FYEVMSYVTHLSV 141

  Fly   191 KDIGDLEKEFWRLKNTS---QNGQIDLQFLGPLISPPIPKNALAGLFNAFDENRDGHIDFKELCC 252
            .::.:|||.|..:.:.:   .|.:...|.||..    .|.:....||..|||||||.|||:||.|
Worm   142 NEVIELEKVFATISDRAVCKLNEEKWKQALGGC----FPDSYAERLFAVFDENRDGQIDFRELVC 202

  Fly   253 GVSAACRGPGVERTRFCFKIFDVDRDGVLSHDETLQMINVLLLVAKENQESQQYKDLTKQLVISD 317
            .:||.||||...|.....:|:|||.|.:||.:|.                |..||||
Worm   203 TLSALCRGPLPGRISQLARIWDVDCDKLLSDEEL----------------SNMYKDL-------- 243

  Fly   318 LLEFGQRRSPDGTPSKLTRDNVSLTAEDFMLWTVQCDLRLMQPLLDLIFELCHIVFGLWPQCKHM 382
                   ..|:...:.....|......||.:| .|.:.:.:.....:..::.||..||.|:.:.|
Worm   244 -------NVPEEHQTVTKSSNGKSALVDFGIW-AQENEKYVNEYYSMALQIGHICLGLRPESRKM 300

  Fly   383 ENDIVRGWLRREERRPYRVGQFWYLITHDWWLSWMQYTQHTTHTCDYCKRTASQRTAVDEALVCD 447
            |..||..:.:|....|.   ..|.::...|......:                        |..|
Worm   301 ELQIVNEFEKRASELPL---SEWNIVASGWHAELRSF------------------------LEAD 338

  Fly   448 ESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGRHCGPVRPGPIDNSNLITANP---FRNVR 509
            ::                                         |.|||||.:.....   ...|.
Worm   339 KN-----------------------------------------PNPIDNSGIKGTREDSWTSKVA 362

  Fly   510 TLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNRWYGDNLPLPRQVIQP-------PNSDVELEL 567
            .::.|...||.|  |:.: |:..||..||:|..||:|..|.:..|..:.       .::...|||
Worm   363 CISAESARLKPD--LIPS-DYIRVPVPLWRAWLRWHGCALTVDSQFTRKYLDGEFFEDNKPALEL 424

  Fly   568 YPLNLRILLHQAQPSQTGVGGGTQ-LGSWGSTVSGGYGVLASGGGYAAIAVSSVLQPPKRYLAYT 631
            |||.:.:|.|..:.||.|.....: |.||.                                  .
Worm   425 YPLEILLLGHDRKKSQDGTENTPRSLTSWA----------------------------------C 455

  Fly   632 AAFSRLATVRQVGEFLCEQLRLKSEDIRLWH-VPQLDNGAILLEEDAMCLKELLIR-------DN 688
            |..||..||.::......:|||...|.|||. |.:.:.|.:||::.|..|.:|...       :.
Worm   456 AQVSRSMTVDELLALCKTELRLGDGDARLWQVVKENEEGNVLLDDGAQNLHQLYSSLGKTKKVNK 520

  Fly   689 DQLLLEIRNKDL-TWPEELGSLATAQCGQGAGTPGDRRRLTRSSIMSVHAPGATGLHNLGNTCFM 752
            .:||||:|.:.. .|||||.:..:.:....|.|.....:|:.||    ..|||.||.|.||.|:.
Worm   521 MKLLLEVRERGTGVWPEELRASLSGKQITAASTLSSNAQLSDSS----GRPGAVGLVNYGNFCYR 581

  Fly   753 NAALQVLFNTQPLAQYFQREMHRFEVNAAN-KLGTKGQLAMRYAELLKEVWTATTRSVAPLKLRF 816
            ||.:|.|....||.|||..|.:...:...| :.|...:..:.||:||:|:|.|..:::||.....
Worm   582 NAGIQCLARVSPLTQYFLDEDNLDAIKRGNLRRGDAAETTIEYAKLLREMWAAKKKNIAPNDFND 646

  Fly   817 CVNKYAPQFAGGGQHDSQELLEWLLDALHEDL---NRVMEKPYSE--------LKDSNGR----- 865
            .:...:..|....|||.||.:.:|||.||..:   |:.:.....|        |.||:.:
Worm   647 AIRLSSDMFECSEQHDCQEFVAFLLDQLHTSMYESNKSLHPSPEESEGTDSNKLSDSSKKKEADK 711

  Fly   866 --PDKIVAAEAWSQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYF 928
              .|:..|..:|:::..:|:|::..||.|||:|::.|..|...|..|:||:.||||:..|:...:
Worm   712 EEADEEKAERSWTEYEKQNESLVTQLFTGQLRSRLICRTCQSSSSVFEPFTSLSLPIGFEDVDLY 776

  Fly   929 EVLVILLDGSVPIKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLRT 993
            :|:|:..||.:|.:|||||:.|.|..:|:..::.
Worm   777 QVIVVHRDGRIPRRYGFRLSRDSKVGNLREVVAV------------------------------- 810

  Fly   994 QSAKELYVYQLPEQSMRTRSNSGLSM-HIEQGLKDIQRSSALITSAQDSLSSLSTLQTSS--HRA 1055
                                :||:|| |:     .||           .:||..||.:.|  ||:
Worm   811 --------------------SSGISMSHL-----TIQ-----------CMSSKGTLMSRSPNHRS 839

  Fly  1056 SSRVLCNGHVSGLDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRC 1120
            |:
Worm   840 SN--------------------------------------------------------------- 841

  Fly  1121 FGKRECMPHSLFCFKESLILSSSPENTFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSM 1185
                         .::.|.|||.|               |.|:|...|
Worm   842 -------------LRDELPLSSFP---------------SGARLYALE----------------- 861

  Fly  1186 ESSLTEPIPLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQ 1250
               |.|               |.|||
Worm   862 ---LPE---------------STGED--------------------------------------- 869

  Fly  1251 PDQITTSQPETSSGVYSRRSSQPPHKAGKYLVAVHRKIT-RHDSYFL-SYHKTRPSLFGVPLLIP 1313
                                        ::.||:|||:. .|:.|.| |......|.||:||::.
Worm   870 ----------------------------QWRVAMHRKLQYNHEPYILGSTAGFIVSRFGLPLIVG 906

  Fly  1314 NSEGGTHKDLYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPW 1378
            ..|..|.|.||..|..|:.|.:.....::....|....|::.||  ||||..|..:...|..||.
Worm   907 LDEEVTGKKLYEDVMYQMHRFMEHSVNSSSSRAHDPCEDENSGY--PFTLCLVDPNYEWCGQCPA 969

  Fly  1379 SSFCRGCEIRCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTK 1443
            ..|||||.||.:...|...|..|
Worm   970 LRFCRGCPIRPDESKVFIPANCP------------------------------------------ 992

  Fly  1444 YFEDFTIAIDWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKLEQWYH 1508
                  ||:||.|.||:|||..:.|:...|..::|.:......|..|.||:..|:..|.|:....
Worm   993 ------IAVDWLPIALYLRYNHSQEQACEDDPSVAETWSRHFAPSSLEHCIEKFSCPETLDAAIQ 1051

  Fly  1509 CSHCKGKKPATKKLQIWKLPPILIVHLKRFNCV--NGKWVKSQKVVHFPFDDFDPTPYLASVPQE 1571
            |..|:.|....|.:.|||||..||:|||||..:  .|:..|.::.|:||...|||.|:
Worm  1052 CDRCEKKTMRDKVMTIWKLPKYLIIHLKRFEFLREQGRMGKCKRTVNFPLKHFDPAPF------- 1109

  Fly  1572 TILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQS 1636
                                          :|.|.           |:|
Worm  1110 ------------------------------VDKPD-----------GNT---------------- 1117

  Fly  1637 KTKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISY 1701
                                                        |...|:.:|.|.|:.||:|:|
Worm  1118 --------------------------------------------YECIALANHYGQLSCGHFIAY 1138

  Fly  1702 ASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERK 1739
            |.:....|...||.|.||:|::.| |...|||||||||
Worm  1139 AKSNEDKWLLLNDCSVREVSEEEV-DKQGAYLLFYERK 1175

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	FRQ1	186..317	CDD:227455	47/133 (35%)
		UBP12	486..>1002	CDD:227847	152/554 (27%)
		Peptidase_C19	<1480..1737	CDD:351799	60/258 (23%)
cyk-3	NP_001022992.1	EFh_PEF	23..201	CDD:330173	59/235 (25%)
		EF-hand_7	177..241	CDD:316058	31/79 (39%)
		Peptidase_C19	322..1175	CDD:321984	304/1377 (22%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	44	1.000	Domainoid score	I8395
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H13066
Inparanoid	1	1.050	345	1.000	Inparanoid score	I1375
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D1283205at2759
OrthoFinder	1	1.000	-	-		FOG0007271
OrthoInspector	1	1.000	-	-		oto17884
orthoMCL	1	0.900	-	-		OOG6_104647
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X4685
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	10	9.870