Protein Alignment Usp32 and F07A11.4

DIOPT Version :10

• Sequence 1: NP_001262074.2
• Gene: Usp32 / 40169
• FlyBaseID: FBgn0036913
• Length: 1779
• Species: Drosophila melanogaster
• Sequence 2: NP_001364591.1
• Gene: F07A11.4 / 174780
• WormBaseID: WBGene00008547
• Length: 1135
• Species: Caenorhabditis elegans
• Alignment Length: 1409
• Identity: 283/1409 - (20%)
• Similarity: 452/1409 - (32%)
• Gaps: 507/1409 - (35%)

  Fly   420 TQHTTHTCDYCKRTASQRTAVDEALVCDESFNTHSLEQHDSYSLGSGTGSASGSGSASSGISAGR 484
            |.|.|:      |..|:.:.::.:.|.|..         ..||        |.:.|.||...:.|
 Worm   120 TPHNTY------RGISKYSTIERSTVADTK---------PYYS--------SATSSLSSTYDSRR 161

  Fly   485 HCGPVRPGPIDNSNL----ITANPFRNVRTLTGEGGHLKRDTPLVQNHDFELVPKSLWKALNR-- 543
            |.   |...|.:|.|    |...|......:.....|::..:...:::...:..:...:..:|
 Worm   162 HS---RSDRISSSTLPDVKIETGPTTRTHMVHPTSSHIRSRSSYGRSNTVSMFAQQARERADREE 223

  Fly   544 -------WYGDNLPLPRQVIQPPNSDVELE-LYPLNLRILLHQAQ----------PSQTGVGGGT 590
                   |        .|..:...:::.|| |||........|:.          ||.|.....|
 Worm   224 KDRDYKEW--------MQKEKDRETELGLETLYPTTSSSSSAQSSAQSEFRRSVAPSPTSTSTTT 280

  Fly   591 QLGS--WGSTVSGGYGVLAS------GGGYAAIAVSSVLQPPKRYLAYTAAFSRLATVRQVGEFL 647
            ...|  :.|...||....|:      ...|:::|.:    ||..|...:.:.|.|...|:.
 Worm   281 PYRSNRYSSATRGGNSSSAAPLDDIMSTSYSSVAAT----PPPAYSQLSTSTSTLLRTRRE---- 337

  Fly   648 CEQLRLKSEDIRLWHVPQLDNGAILLEEDAMCLKELLIRDNDQLLLEIRNKDLTWPEELGSL--- 709
                ||:.|:                                    |.||.....|:....:
 Worm   338 ----RLEREE------------------------------------EGRNSSSMTPQPQSLVPPP 362

  Fly   710 --------ATAQCGQGAGTPG--DRRRLTRSSIMSVHAP-------------------GATGLHN 745
                    :.|..|:....|.  ....::.:|..||:.|                   |.|||.|
 Worm   363 PPPPPPPPSAASNGRSGYLPRSVSAAVISSASSSSVYKPTAMVLPYEEDREKVCVVEQGYTGLRN 427

  Fly   746 LGNTCFMNAALQVLFNTQPLAQYFQREMHRFEVNAANKLGTKGQLAMRYAELLKEVWTATTRSVA 810
            :||||||||.||:|.|...|.:||.|..::.|:|..|.||::|:||..:|:.:.::|:...:::.
 Worm   428 IGNTCFMNAVLQMLVNNIELREYFLRNHYQPEINETNPLGSEGRLAKAFADFMHQMWSGHQKAIE 492

  Fly   811 PLKLRFCVNKYAPQFAGGGQHDSQELLEWLLDALHEDLNRVMEKPYSELKDSNGRPDKIVAAEAW 875
            |.:::..|.:.|.|||...|||:.|.|.:|||.||||:|||.:||.:...:|:||.|..|:.|||
 Worm   493 PTQIKNIVAEKASQFANFAQHDAHEFLSFLLDGLHEDVNRVKKKPLTGTVESHGRHDLDVSNEAW 557

  Fly   876 SQHHARNQSIIIDLFYGQLKSKVSCLGCGHESVRFDPFSLLSLPLPVENYIYFEVLVILLDGSVP 940
            ..|..||.||.:|||:|||||.|.|..|...|:.||||..|.:|.|........:...|.:.:.|
 Worm   558 KNHILRNDSIFVDLFHGQLKSHVQCPNCDRVSITFDPFVYLPVPFPKNKQSTDLIFWPLENQTKP 622

  Fly   941 IKYGFRLNSDCKYSHLKHKLSTMCSLPPNLMLVCELWNSQIRQVLNDDEKLR-TQSAKELYVYQL 1004
            .|.....:::...:.....:|....:|...:..||....:..::.:.|.|:. ..|...|:|:|.
 Worm   623 YKLTVSYSTEGTVADFLSVVSETVRVPTRNLRACEAICHKFDKIYSLDMKVSDIVSPDRLFVFQT 687

  Fly  1005 PEQSMRTRSNSGLSMHIEQGLKDIQRSSALITSAQDSLSSLSTLQTSSHRASSRVLCN--GHVSG 1067
            .::                            ....:.:..|..||...:|.:::..|:  |: |.
 Worm   688 HDE----------------------------MEFNEEIVILHVLQRELYRKNTKHACHECGN-SK 723

  Fly  1068 LDVEGEAEVGTDVSQCNSNSNYNPIVSTYSGNGSGDNQVHELLPDEAGKVSRCFGKR--ECMPHS 1130
            |.::. .|...|...|:....    |:.:|..|..:               .|..::  |.:.|.
 Worm   724 LKLKA-CEECYDAVYCSKECQ----VANWSTGGHRE---------------ECSKRKPSETVGHP 768

  Fly  1131 LFCFKESLILSSSPEN--TFMHGAAAQQKRVSSAKLLHTESNTSSMSYTNHSGENSMESSLTEPI 1193
            |        :.|.|.:  |:.|     ..||..||..||   .:......:..|.|...||..|
 Worm   769 L--------IVSLPRSQLTYQH-----LYRVLEAKSRHT---VTLFQQPQYESEGSEGDSLQAP- 816

  Fly  1194 PLADLEPVSSRNGSGGEDCSYRTSPNDSSGLSTGHTLGASLDVDEQAEEGNAEDHDQPDQITTSQ 1258
                ...:..|||.|... ::...|.:||..|:                            .:|.
 Worm   817 ----SRSIKKRNGVGAPP-TFLQPPGESSSSSS----------------------------VSST 848

  Fly  1259 PETSSGVYSRRS-SQPPHKAGKYLVAVHRKITRHDSYFLSYHKTRPSLFGVPLLIPNSEGGTHKD 1322
            |.       ||| :..|....:.:..:.:...::||:
 Worm   849 PR-------RRSVAAEPRTKNQKMFEIRKLSGQNDSF---------------------------- 878

  Fly  1323 LYCAVWLQVSRLLSPLPATTEQANHAADCDDSLGYDFPFTLRAVKADGLTCAICPWSSFCRGCEI 1387
                                  .:|:...|::                           |:..|
 Worm   879 ----------------------GSHSISDDEA---------------------------CQSLE- 893

  Fly  1388 RCNNDYVLQGALPPINAAASNTSTPKMNAKFPSLPNLEAKRTPEYTASLSYTPTTKYFEDFTIAI 1452
                    .|....:|........|.:        .:|.::..:.....|....::....:
 Worm   894 --------SGGYVSVNWINQRNGKPYI--------TIENRKDIDVDVEKSRQMNSRNMNGY---- 938

  Fly  1453 DWDPTALHLRYQSTLERLWVDHETIAISRREQVEPVDLNHCLRAFTSEEKL---EQWYHCSHCKG 1514
                                     ..|...:..| .|...|..|:..|:|   |.|| ||.||.
 Worm   939 -------------------------VKSNNSETNP-HLTQMLDLFSETERLKPEESWY-CSTCKE 976

  Fly  1515 KKPATKKLQIWKLPPILIVHLKRF-------NCVNGKWVKSQKVVHFPFDDFDPTPYLASVPQET 1572
            ...||||||:::|||:||:.||||       .....:..|..:.|.:|.:..|.:|:||    ||
 Worm   977 NVEATKKLQLYRLPPVLIIQLKRFVYTAFTYQSSMARRSKDTRSVEYPLESLDMSPFLA----ET 1037

  Fly  1573 ILRHKELLELKNDAEMTMATNEVVSELDEIDAPSKEVKEELPNQTGSTKATASPPPTGNILRQSK 1637
                                                                ||.|...:
 Worm  1038 ----------------------------------------------------SPHPNSTM----- 1045

  Fly  1638 TKNAVRRQRLISTSLTKTPIVDGEFEDYHQHRLKPDVDQFDPRYRLYAVVSHSGMLNGGHYISY- 1701
                                                       |.|..||.|||....|||||.
 Worm  1046 -------------------------------------------YDLTGVVCHSGNSYFGHYISMG 1067

  Fly  1702 ------ASNATGSWYCYNDSSCREISQKPVIDPSAAYLLFYERK 1739
                  :|.....|..::||:....|.. .:....||||||:.:
 Worm  1068 RLADFDSSKTKIEWRNFDDSTVARQSTS-TLQTEDAYLLFYKMR 1110

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Usp32	NP_001262074.2	EFh_PI-PLC	198..>251	CDD:333715
		EF-hand motif	198..222	CDD:320029
		EF-hand_7	227..290	CDD:463900
		UBP12	486..>1002	CDD:227847	144/580 (25%)
		Peptidase_C19	<1480..1737	CDD:470612	63/273 (23%)
F07A11.4	NP_001364591.1	UBP12	<401..1110	CDD:227847	222/1044 (21%)
		zf-MYND	716..756	CDD:460312	10/60 (17%)