Protein Alignment Trpml and Mcoln2

DIOPT Version :10

• Sequence 1: NP_649145.1
• Gene: Trpml / 40152
• FlyBaseID: FBgn0262516
• Length: 652
• Species: Drosophila melanogaster
• Sequence 2: NP_080932.2
• Gene: Mcoln2 / 68279
• MGIID: 1915529
• Length: 566
• Species: Mus musculus
• Alignment Length: 550
• Identity: 211/550 - (38%)
• Similarity: 324/550 - (58%)
• Gaps: 39/550 - (7%)

  Fly    75 GREEQPEFPG--------------SSAAS----YQEERMRRKLQFFFMNPIEKWQAKRKFPYKFV 121
            |.||..:.|.              |:.||    .:||.:|..|:|:||:|.||::|:|:.|:|..
Mouse     3 GDEETLDLPAWNRVPDLTWGPHHRSAMASLDSEVREECLREDLKFYFMSPCEKYRARRQIPWKLG 67

  Fly   122 VQIVKIFLVTMQLCLFAHSRYNHINYTGDNRFAFSHLFLRGWDSSREVESYPPAVGPFALYLKSE 186
            :||:||.:||.||..|..|....:.:..||..||.||||:|: |..:.:.|     ..::|.:..
Mouse    68 LQILKIVMVTTQLVRFGLSNQLVVAFKEDNTVAFKHLFLKGF-SGVDEDDY-----SCSIYTQEN 126

  Fly   187 FFDTVQYAVNGYANVSR-SIGPYDYPTPNNTMPPLKLCLQNYREGTIFGFNESYIFDPHIDEVCE 250
            .::::.:|:..|.::.. |:....|....:....||:|.|:|:.|.:|..||:...|..|:..|.
Mouse   127 TYESIFFAIKQYRHLKNISLATLGYGESEDNRTGLKVCKQHYKTGAMFSSNETLNIDSDIETDCI 191

  Fly   251 RLPPNVTTIGVENYLRQRDVEVNFASLVSAQLTFKIKTVNFKANGGPLSAPDCFRFDISITFNNR 315
            .|...|.|...|::.:.....::|..||...::|.:|.::.:|... ...|||:.|..:|||:|.
Mouse   192 HLDLQVLTTEPEDWAQTSFFRLDFYRLVQVDISFALKGIDLQAVHS-REIPDCYLFQNTITFDNT 255

  Fly   316 DHDGQMLLSLDAEATRLKCHGATDFISDANFDSMLRS-----VLNIFVLLTCALSFALCTRALWR 375
            .|.|::.:.|::||...:|....  ||    .|..||     |.::||::.|..|..||||::..
Mouse   256 AHSGKIKIYLNSEANIEECKNMN--IS----GSTQRSTHYLLVFDVFVIMICLASLILCTRSIVL 314

  Fly   376 AYLLRCTTVNFFRSQFGKELSFDGRLEFVNFWYIMIIFNDVLLIIGSALKEQIEGRYLVVDQWDT 440
            |..||...:|||..::.:.:....:.||||.||:::..:|::.||||.||.:|:.:.|.  .:|.
Mouse   315 ALRLRKRFLNFFLEKYKQRVCGADQWEFVNGWYVLVTISDLMTIIGSILKMEIKAKKLT--NYDV 377

  Fly   441 CSLFLGIGNLLVWFGVLRYLGFFKTYNVVILTLKKAAPKILRFLIAALLIYAGFVFCGWLILGPY 505
            ||:.||...|.||.||:||||:|:||||:|||::.:.||:|||...|.:||.|:.||||::||||
Mouse   378 CSILLGTSTLFVWVGVIRYLGYFQTYNVLILTMQASLPKVLRFCACAGMIYLGYTFCGWIVLGPY 442

  Fly   506 HMKFRSLATTSECLFALINGDDMFATFATLSSKATWLWWFCQIYLYSFISLYIYVVLSLFIAVIM 570
            |.||.:|...:||||:|:||||||||||.:..|:..:|.|.::||||||||:||:|||||||:|.
Mouse   443 HEKFENLNIVAECLFSLVNGDDMFATFAQIQQKSILVWLFSRLYLYSFISLFIYMVLSLFIALIT 507

  Fly   571 DAYDTIKAYYKDGFPTTDLKAFVGTRTAED 600
            |:|.|||.|.:.|||.|||:.|:....::|
Mouse   508 DSYHTIKKYQQHGFPETDLQKFLKESGSKD 537

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Trpml	NP_649145.1	ELD_TRPML	174..335	CDD:410965	40/161 (25%)
		Ion_trans	<403..578	CDD:459842	97/174 (56%)
Mcoln2	NP_080932.2	Extracellular/lumenal pore loop. /evidence=ECO:0000250|UniProtKB:Q9GZU1	107..123		3/21 (14%)
		ELD_TRPML	111..276	CDD:425394	42/170 (25%)
		Ion_trans	292..518	CDD:459842	115/227 (51%)
		Selectivity filter. /evidence=ECO:0000250|UniProtKB:F6RG56	461..464		2/2 (100%)