DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Trpml and MCOLN1

DIOPT Version :9

Sequence 1:	NP_649145.1	Gene:	Trpml / 40152	FlyBaseID:	FBgn0262516	Length:	652	Species:	Drosophila melanogaster
Sequence 2:	NP_065394.1	Gene:	MCOLN1 / 57192	HGNCID:	13356	Length:	580	Species:	Homo sapiens

Alignment Length:	632	Identity:	234/632 - (37%)
Similarity:	351/632 - (55%)	Gaps:	97/632 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly    51 STPVAP--------VVPMP---ISAGSGTAPPSVDGREEQPEFPGSSAASYQEERMRRKLQFFFM 104
            :.|..|        :.|.|   ..||...|||:      .||          ||.:||:|::|||
Human     2 TAPAGPRGSETERLLTPNPGYGTQAGPSPAPPT------PPE----------EEDLRRRLKYFFM 50

  Fly   105 NPIEKWQAKRKFPYKFVVQIVKIFLVTMQLCLFAHSRYNHINYTGDNRFAFSHLFLRGWDSSREV 169
            :|.:|::||.:.|.|.::|:|||.:||:||.||..|....:.:..:|..||.||||.|:...
Human    51 SPCDKFRAKGRKPCKLMLQVVKILVVTVQLILFGLSNQLAVTFREENTIAFRHLFLLGYSDG--- 112

  Fly   170 ESYPPAVGPFALYLKSEFFDTVQYAVNGY-ANVSRSIGPYDY------PTPNNTMPPLKLCLQNY 227
                 |...||.|.:.:.:..:.:||:.| |....|:|.|.|      |..|.:  .|.||.:.|
Human   113 -----ADDTFAAYTREQLYQAIFHAVDQYLALPDVSLGRYAYVRGGGDPWTNGS--GLALCQRYY 170

  Fly   228 REGTIFGFNESYIFDPHIDEVC------ERLPP----NVTTIGVENYLRQRDVEVNFASLVSAQL 282
            ..|.:...|:::..||.:...|      ||.||    ::|.:  |:....:::.:.|..||:..:
Human   171 HRGHVDPANDTFDIDPMVVTDCIQVDPPERPPPPPSDDLTLL--ESSSSYKNLTLKFHKLVNVTI 233

  Fly   283 TFKIKTVNFKA--NGGPLSAPDCFRFDISITFNNRDHDGQMLLSLDAEATRLKC-------HGAT 338
            .|::||:|.::  |.   ..|||:.|.:.|||:|:.|.|::.:||:.:|...:|       ||
Human   234 HFRLKTINLQSLINN---EIPDCYTFSVLITFDNKAHSGRIPISLETQAHIQECKHPSVFQHG-- 293

  Fly   339 DFISDANFDSMLRSVLNIFVLLTCALSFALCTRALWRAYLLRCTTVNFFRSQFGKELSFDGRLEF 403
                    |:..|.:.::.|:|||:|||.||.|:|.|.:||:...|.|...|.|:.:|...||||
Human   294 --------DNSFRLLFDVVVILTCSLSFLLCARSLLRGFLLQNEFVGFMWRQRGRVISLWERLEF 350

  Fly   404 VNFWYIMIIFNDVLLIIGSALKEQIEGRYLVVDQWDTCSLFLGIGNLLVWFGVLRYLGFFKTYNV 468
            ||.|||:::.:|||.|.|:.:|..||.:.|.  .:|.||:.||...||||.||:|||.||..||:
Human   351 VNGWYILLVTSDVLTISGTIMKIGIEAKNLA--SYDVCSILLGTSTLLVWVGVIRYLTFFHNYNI 413

  Fly   469 VILTLKKAAPKILRFLIAALLIYAGFVFCGWLILGPYHMKFRSLATTSECLFALINGDDMFATFA 533
            :|.||:.|.|.::||.....:||.|:.||||::|||||:|||||:..|||||:||||||||.|||
Human   414 LIATLRVALPSVMRFCCCVAVIYLGYCFCGWIVLGPYHVKFRSLSMVSECLFSLINGDDMFVTFA 478

  Fly   534 TLSS---KATWLWWFCQIYLYSFISLYIYVVLSLFIAVIMDAYDTIKAYYKDGFPTTDLKAFVGT 595
            .:.:   :::.:|.|.|:||||||||:||:|||||||:|..||||||.....|...::|:|:: .
Human   479 AMQAQQGRSSLVWLFSQLYLYSFISLFIYMVLSLFIALITGAYDTIKHPGGAGAEESELQAYI-A 542

  Fly   596 RTAEDISSGVFMTDLDDFDQTSFLDVVKSICCCGRCGRHQEPAQPNS 642
            :..:..:||.|        :.........:|||||     :|::.:|
Human   543 QCQDSPTSGKF--------RRGSGSACSLLCCCGR-----DPSEEHS 576

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Trpml	NP_649145.1	Ion_trans	403..578	CDD:278921	99/177 (56%)
MCOLN1	NP_065394.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..38		11/51 (22%)
		Dileucine motif, mediates targeting to lysosomes. /evidence=ECO:0000269\|PubMed:16497227	11..16		0/4 (0%)
		Interaction with phosphoinositides. /evidence=ECO:0000269\|PubMed:22733759	42..62		10/19 (53%)
		Extracellular/lumenal pore loop. /evidence=ECO:0000305\|PubMed:28112729	107..121		4/21 (19%)
		ELD_TRPML1	112..292	CDD:410966	51/194 (26%)
		PKD_channel	<388..524	CDD:400395	79/135 (59%)
		Selectivity filter. /evidence=ECO:0000305\|PubMed:29019983	469..474		4/4 (100%)
		Required for palmitoylation and association with membranes. /evidence=ECO:0000269\|PubMed:16497227	565..567		1/1 (100%)
		Dileucine internalization motif, mediates AP2 complex-dependent internalization. /evidence=ECO:0000269\|PubMed:16497227	573..578		1/4 (25%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165156402
Domainoid	1	1.000	208	1.000	Domainoid score	I2860
eggNOG	1	0.900	-	-		E1_KOG3733
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	415	1.000	Inparanoid score	I1837
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49191
OrthoDB	1	1.010	-	-		D1379516at2759
OrthoFinder	1	1.000	-	-		FOG0001492
OrthoInspector	1	1.000	-	-		otm41429
orthoMCL	1	0.900	-	-		OOG6_102454
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3353
SonicParanoid	1	1.000	-	-		X949
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	14	13.700