DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Trpml and MCOLN2

DIOPT Version :9

Sequence 1:	NP_649145.1	Gene:	Trpml / 40152	FlyBaseID:	FBgn0262516	Length:	652	Species:	Drosophila melanogaster
Sequence 2:	NP_694991.2	Gene:	MCOLN2 / 255231	HGNCID:	13357	Length:	566	Species:	Homo sapiens

Alignment Length:	564	Identity:	212/564 - (37%)
Similarity:	339/564 - (60%)	Gaps:	46/564 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly    91 QEERMRRKLQFFFMNPIEKWQAKRKFPYKFVVQIVKIFLVTMQLCLFAHSRYNHINYTGDNRFAF 155
            :||.:|..|:|:||:|.||::|:|:.|:|..:||:||.:||.||..|..|....:.:..||..||
Human    37 KEECLREDLKFYFMSPCEKYRARRQIPWKLGLQILKIVMVTTQLVRFGLSNQLVVAFKEDNTVAF 101

  Fly   156 SHLFLRGWDSSREVESYPPAVGPFALYLKSEFFDTVQYAVNGYANVSR-SIGPYDYPTPNNTMPP 219
            .||||:|:..:.| :.|..:|     |.:.:.::::.:|:|.|..:.. ::|...|....:....
Human   102 KHLFLKGYSGTDE-DDYSCSV-----YTQEDAYESIFFAINQYHQLKDITLGTLGYGENEDNRIG 160

  Fly   220 LKLCLQNYREGTIFGFNESYIFDPHIDEVCERL--------PPNVTTIGVENYLRQRDVEVNFAS 276
            ||:|.|:|::||:|..||:...|..::..|.:|        ||:...   .::.|     :.|..
Human   161 LKVCKQHYKKGTMFPSNETLNIDNDVELDCVQLDLQDLSKKPPDWKN---SSFFR-----LEFYR 217

  Fly   277 LVSAQLTFKIKTVNFKANGGPLSAPDCFRFDISITFNNRDHDGQMLLSLDAEATRLKCHGATDFI 341
            |:..:::|.:|.::.:.... ...|||:.|..:|.|:|:.|.|::.:..|::|...:|.....|.
Human   218 LLQVEISFHLKGIDLQTIHS-RELPDCYVFQNTIIFDNKAHSGKIKIYFDSDAKIEECKDLNIFG 281

  Fly   342 S-DANFDSMLRSVLNIFVLLTCALSFALCTRALWRAYLLRCTTVNFFRSQFGKELSFDGRLEFVN 405
            | ..|...:|  |.:.||::.|..|..||||::..|..||...:|||..::.:.:....:.||:|
Human   282 STQKNAQYVL--VFDAFVIVICLASLILCTRSIVLALRLRKRFLNFFLEKYKRPVCDTDQWEFIN 344

  Fly   406 FWYIMIIFNDVLLIIGSALKEQIEGRYLVVDQWDTCSLFLGIGNLLVWFGVLRYLGFFKTYNVVI 470
            .||:::|.:|::.||||.||.:|:.:.|.  .:|.||:|||...||||.||:||||:|:.|||:|
Human   345 GWYVLVIISDLMTIIGSILKMEIKAKNLT--NYDLCSIFLGTSTLLVWVGVIRYLGYFQAYNVLI 407

  Fly   471 LTLKKAAPKILRFLIAALLIYAGFVFCGWLILGPYHMKFRSLATTSECLFALINGDDMFATFATL 535
            ||::.:.||:|||...|.:||.|:.||||::|||||.||.:|.|.:||||:|:||||||||||.:
Human   408 LTMQASLPKVLRFCACAGMIYLGYTFCGWIVLGPYHDKFENLNTVAECLFSLVNGDDMFATFAQI 472

  Fly   536 SSKATWLWWFCQIYLYSFISLYIYVVLSLFIAVIMDAYDTIKAYYKDGFPTTDLKAFVGTRTAED 600
            ..|:..:|.|.::||||||||:||::||||||:|.|:|||||.:.::|||.|||:.|:     ::
Human   473 QQKSILVWLFSRLYLYSFISLFIYMILSLFIALITDSYDTIKKFQQNGFPETDLQEFL-----KE 532

  Fly   601 ISSGVFMTDLDDF--DQTSFLDVVKSICCCGRCGRHQEPAQPNS 642
            .||      .:::  :.::||    |..||.|..|..:...|.|
Human   533 CSS------KEEYQKESSAFL----SCICCRRRKRSDDHLIPIS 566

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Trpml	NP_649145.1	Ion_trans	403..578	CDD:278921	99/174 (57%)
MCOLN2	NP_694991.2	Extracellular/lumenal pore loop. /evidence=ECO:0000250\|UniProtKB:Q9GZU1	107..123		4/21 (19%)
		PKD_channel	<388..513	CDD:332708	76/124 (61%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:F6RG56	461..464		2/2 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C165156401
Domainoid	1	1.000	208	1.000	Domainoid score	I2860
eggNOG	1	0.900	-	-		E1_KOG3733
Hieranoid	1	1.000	-	-
Homologene	0	0.000	Not matched by this tool.
Inparanoid	1	1.050	415	1.000	Inparanoid score	I1837
Isobase	0	0.000	Not matched by this tool.
OMA	1	1.010	-	-		QHG49191
OrthoDB	1	1.010	-	-		D1379516at2759
OrthoFinder	1	1.000	-	-		FOG0001492
OrthoInspector	1	1.000	-	-		otm41429
orthoMCL	1	0.900	-	-		OOG6_102454
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	1	1.000	-	-		X949
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	13	12.670