DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Trpml and Mcoln3

DIOPT Version :9

Sequence 1:	NP_649145.1	Gene:	Trpml / 40152	FlyBaseID:	FBgn0262516	Length:	652	Species:	Drosophila melanogaster
Sequence 2:	NP_598921.1	Gene:	Mcoln3 / 171166	MGIID:	1890500	Length:	553	Species:	Mus musculus

Alignment Length:	565	Identity:	232/565 - (41%)
Similarity:	349/565 - (61%)	Gaps:	40/565 - (7%)

- Green bases have known domain annotations that are detailed below.

  Fly    76 REEQP-EFPGSSAASYQ---EERMRRKLQFFFMNPIEKWQAKRKFPYKFVVQIVKIFLVTMQLCL 136
            ::|.| .|..||:.|.|   |::|||||:||||||.||:.|:.:.|:|..:||:||.:||:||.|
Mouse    15 QDESPCTFHPSSSPSEQLLLEDQMRRKLKFFFMNPCEKFWARGRKPWKLAIQILKIAMVTIQLVL 79

  Fly   137 FAHSRYNHINYTGDNRFAFSHLFLRGWDSSREVESYPPAVGPFALYLKSEFFDTVQYAVNGYANV 201
            |..|....:.:..:|..||.||||:|: ..|..::|       |:|.:||.:|.:.:||..|..:
Mouse    80 FGLSNQMVVAFKEENTIAFKHLFLKGY-MDRMDDTY-------AVYTQSEVYDQIIFAVTQYLQL 136

  Fly   202 SR-SIGPYDYPTPNNTMPPLKLCLQNYREGTIFGFNESYIFDPHIDEVCERLPPN-VTTIGV--E 262
            .. |:|.:.|.........:.:|...||:|||...|:::..||.::..|..:.|: .:.:|.  |
Mouse   137 QNISVGNHAYENKGTKQSAMAICQHFYRQGTICPGNDTFDIDPEVETECFLVEPDEASHLGTPGE 201

  Fly   263 NYLRQRDVEVNFASLVSAQLTFKIKTVNFKANGGPLSAPDCFRFDISITFNNRDHDGQMLLSLDA 327
            |.|   ::.::|..|::.:|.||:|.:|.:..... ..|||:.|.::|||:|:.|.|::.:|||.
Mouse   202 NKL---NLSLDFHRLLTVELQFKLKAINLQTVRHQ-ELPDCYDFTLTITFDNKAHSGRIKISLDN 262

  Fly   328 EATRLKC---HGATDFISDANFDSMLRSVLNIFVLLTCALSFALCTRALWRAYLLRCTTVNFFRS 389
            :.:..:|   |.:.....:.::    ..:.:.||:|||..|..||.|::.|...|:...||||..
Mouse   263 DISIKECKDWHVSGSIQKNTHY----MMIFDAFVILTCLASLVLCARSVIRGLQLQQEFVNFFLL 323

  Fly   390 QFGKELSFDGRLEFVNFWYIMIIFNDVLLIIGSALKEQIEGRYLVVDQWDTCSLFLGIGNLLVWF 454
            .:.||:|...::||:|.||||||.:|:|.|:||.||.:|:.:.|.  .:|.||:.||...:|||.
Mouse   324 HYKKEVSASDQMEFINGWYIMIIISDILTIVGSVLKMEIQAKSLT--SYDVCSILLGTSTMLVWL 386

  Fly   455 GVLRYLGFFKTYNVVILTLKKAAPKILRFLIAALLIYAGFVFCGWLILGPYHMKFRSLATTSECL 519
            ||:||||||..||::||||:.|.|.::||...|.:||.|:.||||::|||||.|||||...||||
Mouse   387 GVIRYLGFFAKYNLLILTLQAALPNVMRFCCCAAMIYLGYCFCGWIVLGPYHEKFRSLNRVSECL 451

  Fly   520 FALINGDDMFATFATLSSKATWLWWFCQIYLYSFISLYIYVVLSLFIAVIMDAYDTIKAYYKDGF 584
            |:||||||||:|||.:..|:..:|.|.::||||||||:||::||||||:|.|.|:|||.|.:|||
Mouse   452 FSLINGDDMFSTFAKMQQKSYLVWLFSRVYLYSFISLFIYMILSLFIALITDTYETIKHYQQDGF 516

  Fly   585 PTTDLKAFVGTRTAEDI-SSGVFMTDLDDFDQTSFLDVVKSICCC 628
            |.|:|:.|:.  ..:|: :||.:.  |:|....|.|      |||
Mouse   517 PETELRKFIA--ECKDLPNSGKYR--LEDDPPGSLL------CCC 551

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Trpml	NP_649145.1	Ion_trans	403..578	CDD:278921	101/174 (58%)
Mcoln3	NP_598921.1	Interaction with phosphoinositides. /evidence=ECO:0000250\|UniProtKB:F6RG56	52..62		3/9 (33%)
		Extracellular/lumenal pore loop. /evidence=ECO:0000250\|UniProtKB:Q9GZU1	104..118		4/21 (19%)
		PKD_channel	<343..508	CDD:369653	95/166 (57%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:F6RG56	456..459		2/2 (100%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167846806
Domainoid	1	1.000	213	1.000	Domainoid score	I2739
eggNOG	1	0.900	-	-		E1_KOG3733
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H10118
Inparanoid	1	1.050	418	1.000	Inparanoid score	I1789
Isobase	1	0.950	-	0	Normalized mean entropy	S4518
OMA	1	1.010	-	-		QHG49191
OrthoDB	1	1.010	-	-		D1379516at2759
OrthoFinder	1	1.000	-	-		FOG0001492
OrthoInspector	1	1.000	-	-		otm43480
orthoMCL	1	0.900	-	-		OOG6_102454
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3353
SonicParanoid	1	1.000	-	-		X949
SwiftOrtho	1	1.000	-	-
TreeFam	1	0.960	-	-
	17	16.650