DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ash1 and Setd1a

DIOPT Version :9

Sequence 1:	NP_001246834.1	Gene:	ash1 / 40133	FlyBaseID:	FBgn0005386	Length:	2226	Species:	Drosophila melanogaster
Sequence 2:	NP_821172.2	Gene:	Setd1a / 233904	MGIID:	2446244	Length:	1716	Species:	Mus musculus

Alignment Length:	1830	Identity:	349/1830 - (19%)
Similarity:	543/1830 - (29%)	Gaps:	679/1830 - (37%)

- Green bases have known domain annotations that are detailed below.

  Fly   140 DDLPLKVHQQRAPRVLLSAIIQAAQSASKPTL-DIGISSSDNELPNLVQAAI-----KRVESDTE 198
            :::.:.:|.:....:.|:.::..:...:|.|: ::.::|   .:.|::.|.:     :|::  ..
Mouse   121 EEVEILLHPRTRKHLGLARVLFTSTRGAKETVKNLHLTS---VMGNIIHAQLDIKGQQRMK--YY 180

  Fly   199 DTTVEGSFRKAAKDKNLPQYQSTLLQDFMEKTQMLGQTVNAKLAEEKVAKAKEETLVQTAVPRKR 263
            :..|.||:        .||...|..:...||.|..|                  ...:|...|:|
Mouse   181 ELIVNGSY--------TPQTVPTGGKALSEKFQGSG------------------AAAETTEARRR 219

  Fly   264 RGRPKKVVP---TV-PAPGNSGPAINESADSGVISTTSTTQSTTPSPKMQNENAVP---TGSLPI 321
            ........|   || ..|||..|.   |.|:...|:...|.|:......|:....|   .||.|.
Mouse   220 SSSDTAAYPAGTTVGGTPGNGTPC---SQDTNFSSSRQDTPSSFGQFTPQSSQGTPYTSRGSTPY 281

  Fly   322 ASSSKPKIDMAYLDKRMYATERVLYPPPRSKRRQNNKKTACSSSNKEELQLDPLWREIDVNKKFR 386
            :.      |.||..    :|....:.|.||                          |......|.
Mouse   282 SQ------DSAYSS----STTSTSFKPRRS--------------------------ENSYQDSFS 310

  Fly   387 LRSMSVGAASGTGASTTICSKVLAAKSGYVSDYGSVRHQRSSHNHNSGYK-SDASCKSRYSTKSC 450
            .|..|..:|..|.|:.|..:....|.|...|...|.....||.:..|.:: ||:|..:.|.:
Mouse   311 RRHFSTSSAPATTATATSATAAATAASSSSSSSSSSSSSSSSSSSASQFRGSDSSYPAYYES--- 372

  Fly   451 MSRRSRAKSCGYRSDCKESGKSGLRMRRKRRASMLLKSSADDTVEDQDILQLAGLSLGQSSEESN 515
            .:|..|..|...|...:|. .||             .|.|::|.|.......:.|:...:.....
Mouse   373 WNRYQRHTSYPPRRATRED-PSG-------------ASFAENTAERFPPSYTSYLAPEPNRSTDQ 423

  Fly   516 EYISKPSLKSLPTTSASKKYGEINRYVTTGQYFGRGGSLSATNPDNFISKMMNQRKETPAPSKSS 580
            :|  :|.....|.....:..|........|...|.||.                  ..|:|.:..
Mouse   424 DY--RPPASEAPPPEPPEPGGGGGGSGGGGGGGGGGGG------------------GAPSPEREE 468

  Fly   581 CKIKSRRSSAASMCSSYVSGVSRMRRRHRRKSFSHNKSLNIDSKLLTEIEIITSTFNSRCRIQDD 645
            .:...|.:|.|.      ||.......:....|:.:.||  ||::...::...|.|:......::
Mouse   469 ARTPPRPASPAR------SGSPAPETTNESVPFAQHSSL--DSRIEMLLKEQRSKFSFLASDTEE 525

  Fly   646 RLTGSSGKEKLLADANKLQATLAAPSPAQQLTLNGGGPASTLSKPLKRGLKKRKLSEPLVDFAML 710
            ....||      |......|....||.|      |.||.:....|              .:|..:
Mouse   526 EEENSS------AGPGARDAGAEVPSGA------GHGPCTPPPAP--------------ANFEDV 564

  Fly   711 SASASGTPNG---SGSSNGNTKRRHKKSQSNDSSSPDDH-------------------------- 746
            :.:.||.|..   |..:||..:.....|..:...|.||.
Mouse   565 APTGSGEPGAARESPKANGQNQASPCSSGEDMEISDDDRGGSPPPAPTPPQQPPPPPPPPPPPPP 629

  Fly   747 ----KLPL----KKRHYLLTP---GERPPAE-----------VAFANG-----KLNAEAWAAAAA 784
                .|||    .:..|||.|   |..||..           ..|.|.     :|.|: |..
Mouse   630 PYLASLPLGYPPHQPAYLLPPRPDGPPPPEYPPPPPPPPPHIYDFVNSLELMDRLGAQ-WGG--- 690

  Fly   785 AAKSTASTKSQAQFNARSVKSALTPKKRHLLEQPTSVSGA-----GSSASNSPLRIVVDNNSISG 844
               ...|.:.|.|...|          .|.|.|...::.|     |.:...:.|.......:..|
Mouse   691 ---MPMSFQMQTQMLTR----------LHQLRQGKGLTAASAGPPGGAFGEAFLPFPPPQEAAYG 742

  Fly   845 GKLLDISPSSLCSLKQQRRGGAAKQKVSAAKDLVQLQSPAGSYPPPGVFEPSVEL------EIQI 903
                  .|.:|.:..|:.||       |.:::...|..|..:.|.|.......|.      |.:|
Mouse   743 ------LPYALYTQGQEGRG-------SYSREAYHLPLPMAAEPLPSSSVSGEEARLPHREEAEI 794

  Fly   904 PLSKLNESVITKAEVESPLLSALDIKEDTKKEVGQRVVETLLH---------------------- 946
            ..||:..|..|...|.:.|:.  ::|...::::.:::||.:..
Mouse   795 AESKVLPSAGTVGRVLATLVQ--EMKSIMQRDLNRKMVENVAFGAFDQWWESKEEKAKPFQNAAK 857

  Fly   947 -------------------------KTGG--------------------NLLLKRK--------- 957
                                     |:||                    :..:|||
Mouse   858 QQAKEEDKEKMKLKEPGMLSLVDWAKSGGITGIEAFAFGSGLRGALRLPSFKVKRKEPSEISEAS 922

  Fly   958 --------------------RKKINRTGFPTVRRKKR-----KVSVEQQTTAVID---------- 987
                                .|:....|.|..:..||     |...:.:.:..:|
Mouse   923 EEKRPRPSTPAEEDEDDPEREKEAGEPGRPGTKPPKRDEERGKTQGKHRKSFTLDSEGEEASQES 987

  Fly   988 --EHEPEFDPDDEPLQSLRETRSSNNVNVQAAPNPPLDCERVPQA-------GEARETFVARTNQ 1043
              |.:.:.|.:||..:...|...:.....:|:.....|.:...|.       ||...|..:.:..
Mouse   988 SSEKDEDDDDEDEEDEEQEEAVDATKKEAEASDGEDEDSDSSSQCSLYADSDGENGSTSDSESGS 1052

  Fly  1044 KAPRLSVVAL------------ERLQRPQTPARGRPRGRKPKNREQAEAAPQPPPKSEPE----- 1091
            .:...|..:.            |..|....|:...|       ||..|..|.|..|.|.:
Mouse  1053 SSSSSSSSSSSSSSSSSESSSEEEEQSAVIPSASPP-------REVPEPLPAPDEKPETDGLVDS 1110

  Fly  1092 -IRPAKKRGRQPKQPV--LEEPPPTPPPQQKKNKMEPNIRLPDG---IDPNTNFSCKIRL----- 1145
             :.|..::...|.||.  .|||||:.|    :...||....||.   :|...  |..|.|
Mouse  1111 PVMPLSEKETLPTQPAGPAEEPPPSVP----QPPAEPPAGPPDAAPRLDERP--SSPIPLLPPPK 1169

  Fly  1146 KRRKNLE---------------AGTQPKKEKPVQ---PVTVEEIPPEIPVSQ--------EEI-- 1182
            ||||.:.               |..|.|...||.   |..||.....:|:..        ||:
Mouse  1170 KRRKTVSFSAAEEAPVPEPSTAAPLQAKSSGPVSRKVPRVVERTIRNLPLDHASLVKSWPEEVAR 1234

  Fly  1183 -------------------------------------------------DAEA-----EAKR--- 1190
                                                             |:||     ||:|
Mouse  1235 GGRNRAGGRVRSTEEEEATESGTEVDLAVLADLALTPARRGLATLPTGDDSEATETSDEAERPSP 1299

  Fly  1191 -LDSIPTEH------------------DPLPA--------------------SESHNPGPQ---- 1212
             |..|..||                  :|.||                    :|:..|..|
Mouse  1300 LLSHILLEHNYALAIKPPPTTPAPRPLEPAPALAALFSSPADEVLEAPEVVVAEAEEPKQQLQQQ 1364

  Fly  1213 -----------DYASCSESSEDKASTTS-----LRKLSKVKKTYLVAGLFSNHYKQSLMPPPAKV 1261
                       |....|||||..:|::|     :|:.|....|        ...:..|.|||
Mouse  1365 HPEQEGEEEEEDEEEESESSESSSSSSSDEEGAIRRRSLRSHT--------RRRRPPLPPPP--- 1418

  Fly  1262 NKKPGLEEQVGPASLLPPPPYCEKYLRRTEMD-FELPYDIWWAYTNSKLPTRNVVPSWNYRKIR- 1324
                            ||||..|.   |:|.: ..:.||||    ||.|...::    :|.::.
Mouse  1419 ----------------PPPPSFEP---RSEFEQMTILYDIW----NSGLDLEDM----SYLRLTY 1456

  Fly  1325 -----------------------TNVYA--ESVRPNLAGFDHPTCNCKNQG-------EK-SCLD 1356
                                   ||:..  ...||.....:|.|.:.:::|       || ..||
Mouse  1457 ERLLQQTSGADWLNDTHWVQHTITNLSTPKRKRRPQDGPREHQTGSARSEGYYPISKKEKDKYLD 1521

  Fly  1357 NC---LNRMVYTECSPSNCPAGEKCRNQKIQRHAVAPGVERFMTAD------------------- 1399
            .|   ..::...:...:|....|:...|:  |...|.|....|.:|
Mouse  1522 VCPVSARQLEGGDTQGTNRVLSERRSEQR--RLLSAIGTSAIMDSDLLKLNQLKFRKKKLRFGRS 1584

  Fly  1400 --KGWGVRTKLPIAKGTYILEYVGEVV-------TEKEFKQR-MASIYLNDTHHYCLHLDGGLVI 1454
              ..||:....|||....::||||:.:       .||.:.|. :.|.||       ..:|...:|
Mouse  1585 RIHEWGLFAMEPIAADEMVIEYVGQNIRQMVADMREKRYVQEGIGSSYL-------FRVDHDTII 1642

  Fly  1455 DGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGEELTYDYNFSLFNPSEGQ--P 1517
            |..:.|:..||:||.|.|||..:..::....::|:::|:.|...||:||||.|    |.|..  |
Mouse  1643 DATKCGNLARFINHCCTPNCYAKVITIESQKKIVIYSKQPIGVDEEITYDYKF----PLEDNKIP 1703

  Fly  1518 CRCNTPQCRG 1527
            |.|.|..|||
Mouse  1704 CLCGTESCRG 1713

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ash1	NP_001246834.1	AWS	1340..1388	CDD:197795	12/58 (21%)
		SET	1392..1512	CDD:214614	39/148 (26%)
		Bromo_ASH1	1680..1787	CDD:99955
		PHD_ASH1L	1858..1900	CDD:277023
		BAH_polybromo	1929..2073	CDD:240068
Setd1a	NP_821172.2	RRM_Set1A	92..186	CDD:240992	9/69 (13%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	194..367		50/229 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	380..499		26/158 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	516..670		33/179 (18%)
		Topoisomer_IB_N	<835..>883	CDD:322080	0/47 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	849..869		0/19 (0%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	911..1206		60/307 (20%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1230..1259		2/28 (7%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1275..1297		5/21 (24%)
		HCFC1-binding motif (HBM). /evidence=ECO:0000250\|UniProtKB:O15047	1307..1311		2/3 (67%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1355..1427		22/101 (22%)
		Interaction with CFP1. /evidence=ECO:0000250\|UniProtKB:O15047	1424..1459		11/45 (24%)
		N-SET	1434..1571	CDD:314603	28/146 (19%)
		Interaction with ASH2L, RBBP5 and WDR5. /evidence=ECO:0000250\|UniProtKB:O15047	1459..1546		14/86 (16%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1480..1508		6/27 (22%)
		WDR5 interaction motif (WIN). /evidence=ECO:0000250\|UniProtKB:O15047	1501..1506		0/4 (0%)
		SET	1577..1700	CDD:214614	39/133 (29%)
		PostSET	1700..1716	CDD:214703	7/14 (50%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C167848443
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4489
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	3	2.870