back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ash1 and Kmt2a

DIOPT Version :9

Sequence 1:	NP_001246834.1	Gene:	ash1 / 40133	FlyBaseID:	FBgn0005386	Length:	2226	Species:	Drosophila melanogaster
Sequence 2:	NP_001344478.1	Gene:	Kmt2a / 214162	MGIID:	96995	Length:	3966	Species:	Mus musculus

Alignment Length:	1897	Identity:	394/1897 - (20%)
Similarity:	614/1897 - (32%)	Gaps:	549/1897 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly    29 TDSITDQSSQSK--SIKSAT--QFSVQRSDTDGLRMRISAIRPTLGVVATKKPPKSRKMSTQDTE 89
            |.|...:||.|.  |:.:||  :.|.:.||..|| :..||   .||..|   ||.|   |:|.|.
Mouse  2216 TGSAYSRSSVSSVPSLGTATDPEASAKASDRGGL-LSSSA---NLGHSA---PPSS---SSQRTV 2270

  Fly    90 SGCSEAKNRAVSKKVKVKRKKLASSSGISKSDKVSKSKKSQISAFSSDSED------------DL 142
            .| |:..:...|...:|||   .|:|.:.....:.|.:|::.|  ||.|.|            .|
Mouse  2271 GG-SKTSHLDGSSPSEVKR---CSASDLVPKGSLVKGEKNRTS--SSKSTDGSAHSTAYPGIPKL 2329

  Fly   143 PLKVHQQRAPRVLLSAI----------IQAAQSASKPTLDI---------------GISSSDNE- 181
            ..:||......:.:|.|          ..:..:.|.|.|.:               .:..|.||
Mouse  2330 TPQVHNATPGELNISKIGSFAEPSTVPFSSKDTVSYPQLHLRGQRSDRDQHMDPSQSVKPSPNED 2394

  Fly   182 -------LPNL---VQAAIKRVESDTEDTTVEG--SFRKAAKDKNLPQ-----------YQSTLL 223
                   ||.:   .....:.:.|.:.|...:|  |.::..|:|:..:           .:..|.
Mouse  2395 GEIKTLKLPGMGHRPSILHEHIGSSSRDRRQKGKKSSKETCKEKHSSKSYLEPGQVTTGEEGNLK 2459

  Fly   224 QDFMEKT---QMLGQTVNAKLAEEKVAKAKEETLVQTAVPR----------KRRGRPK----KVV 271
            .:|.::.   ..|||.....::.||:.   ::.|..:.||:          |....|:    ||.
Mouse  2460 PEFADEVLTPGFLGQRPCNNVSSEKIG---DKVLPLSGVPKGQSTQVEGSSKELQAPRKCSVKVT 2521

  Fly   272 P-----------TVPAPGNSGPAINES--------------ADSGVISTTSTTQSTTPS------ 305
            |           |....|...||..||              |..||..:.:.|.|..|.
Mouse  2522 PLKMEGENQSKNTQKESGPGSPAHIESVCPAEPVSASRSPGAGPGVQPSPNNTLSQDPQSNNYQN 2586

  Fly   306 -PKMQNENAVPTGSLPIAS----------SSKPKIDMAY-----LDKRMYATERV-LYPPPRSKR 353
             |:......:|.|..|...          |::.:.:|.:     ...|.|..|.: .|.....|:
Mouse  2587 LPEQDRNLMIPDGPKPQEDGSFKRRYPRRSARARSNMFFGLTPLYGVRSYGEEDIPFYSNSTGKK 2651

  Fly   354 R-------------------------QNNKKTACSSSNKEELQLDPLWRE---IDVNKKFRLRSM 390
            |                         .|..:|..||..:|.|....|:||   .|:.|..:|..:
Mouse  2652 RGKRSAEGQVDGADDLSTSDEDDLYYYNFTRTVISSGGEERLASHNLFREEEQCDLPKISQLDGV 2716

  Fly   391 SVGAASGTGASTTICSKVLAAKSGYV------------------SDYGSVRHQ-RSSHNHNSGYK 436
            ..|..|.|..:.|      :.||..:                  .|.|...|. :|:..|.:..|
Mouse  2717 DDGTESDTSVTAT------SRKSSQIPKRNGKENGTENLKIDRPEDAGEKEHVIKSAVGHKNEPK 2775

  Fly   437 SDASCKSRYSTKSCMSRRSRAKSCGYRSDCKESGKSGLRMRRKRRASMLLKSSADDTVEDQDILQ 501
            .| :|.|          .||.|:.|  .|..|:..|.|...|:...|         |..|:::|.
Mouse  2776 LD-NCHS----------VSRVKAQG--QDSLEAQLSSLESSRRVHTS---------TPSDKNLLD 2818

  Fly   502 LAGLSLGQSSEESN--------------EYISK--PSLKSL---PTTSASK--KYGE-----INR 540
            .....|.:|..::|              :::.|  ||:::|   |.:|:|:  ..||     .||
Mouse  2819 TYNAELLKSDSDNNNSDDCGNILPSDIMDFVLKNTPSMQALGESPESSSSELLTLGEGLGLDSNR 2883

  Fly   541 YVTTGQYFGRGGSLSATNP-DNFISKMMN--QRKETPAPSKSSCKIKSRRSSAASMCSSYVSGVS 602
            ....|.:......|.||.| |:.:|..::  ::.|.|....|...:.:.||....
Mouse  2884 EKDIGLFEVFSQQLPATEPVDSSVSSSISAEEQFELPLELPSDLSVLTTRSPTVP---------- 2938

  Fly   603 RMRRRHRRKSFSHNKSLNIDSKLLTEIEIITSTFNSRCRIQDDRLTGSSGKEKLLADANKLQATL 667
                       |.|.|         .:.:|:.:...|..|.:..:..|.|...||:..
Mouse  2939 -----------SQNPS---------RLAVISDSGEKRVTITEKSVASSEGDPALLSPG------- 2976

  Fly   668 AAPSPAQQLT----LNGGGPASTLSKP--------------LKRGLKKRKLSEPL-----VDFAM 709
            ..|:|...:|    :.|...|..:|.|              |.|......|..|:     |....
Mouse  2977 VDPAPEGHMTPDHFIQGHMDADHISSPPCGSVEQGHGNSQDLTRNSGTPGLQVPVSPTVPVQNQK 3041

  Fly   710 LSASASGTPNGSGSSNG--NTKRRHKKSQSNDSSSPDDHKLPL----------KKRHYLLTPGER 762
            ...|::.:|..|..||.  .|...|.|..:......:.:..||          .::..|.:|...
Mouse  3042 YVPSSTDSPGPSQISNAAVQTTPPHLKPATEKLIVVNQNMQPLYVLQTLPNGVTQKIQLTSPVSS 3106

  Fly   763 PPAEVAFANGKLNAEAWAAAAAAAKSTASTKSQAQFNARSVKSALTPKKRHLLEQPTSVSGAGSS 827
            .|:.:......|.............:.:...|.:.|...|......|..:||...|.:...:...
Mouse  3107 TPSVMETNTSVLGPMGSGLTLTTGLNPSLPPSPSLFPPASKGLLSVPHHQHLHSFPAAAQSSFPP 3171

  Fly   828 ASNSPLRIVVDNNSISGGKLLDI----SPSSLCSLKQQR----------RGGAAKQKVSAA---- 874
            ..:||          ..|.|:.:    .|..|.|...||          ..|..|:.:|..
Mouse  3172 NISSP----------PSGLLIGVQPPPDPQLLGSEANQRTDLTTTVATPSSGLKKRPISRLHTRK 3226

  Fly   875 -KDLVQLQSPAGSYPPPGV-------FEPSVELEIQ---IPLSKLNES-------VITKAE---- 917
             |.|....:|:...|...|       |.|| :|...   :.|..||.|       :|.:::
Mouse  3227 NKKLAPSSAPSNIAPSDVVSNMTLINFTPS-QLSNHPSLLDLGSLNPSSHRTVPNIIKRSKSGIM 3290

  Fly   918 --VESPLLSALDIKEDTKKEVGQRVV-ETLLHKTGGNL-LLKRKRKKINRTGFPTVRRKKRKVSV 978
              .::|||....:........|...: :...|.|.|.: .|......:|.....|........||
Mouse  3291 YFEQAPLLPPQSVGGTAATAAGSSTISQDTSHLTSGPVSALASGSSVLNVVSMQTTAAPTSSTSV 3355

  Fly   979 EQQTTAVIDEHEPEFDPDDEPLQSLRETRSSNNVNVQAAP---------NPPLDCERVPQA---- 1030
            ....|  :........||...:..|....|..::.:|..|         .|.|...:.|.|
Mouse  3356 PGHVT--LANQRLLGTPDIGSISHLLIKASHQSLGIQDQPVALPPSSGMFPQLGTSQTPSAAAMT 3418

  Fly  1031 ----------------------GEARETF-VARTNQ-KAPRLSVVALERLQRPQTPARGRPRGRK 1071
                                  |||.|.: :.|.|| .|.:...:..:|.:.|.:..     |.:
Mouse  3419 AASSICVLPSSQTAGMTAASPPGEAEEHYKLQRGNQLLAGKTGTLTSQRDRDPDSAP-----GTQ 3478

  Fly  1072 PKNREQAEAAPQ----PPPKSEPEIRPAKKR--GRQPKQPVLEEPPPTPPPQQKKNKMEPNIRLP 1130
            |.|..|...||.    ...|:.|..:||...  |..|...........|.|.:.|.|.: .|:||
Mouse  3479 PSNFTQTAEAPNGVRLEQNKTLPSAKPASSASPGSSPSSGQQSGSSSVPGPTKPKPKAK-RIQLP 3542

  Fly  1131 DGIDPNTNFSCKI----------------------------RLKRRKNLEAGT-QPKKEKPVQPV 1166
              :|..:....|:                            |..|.:...||. ||.:::..||.
Mouse  3543 --LDKGSGKKHKVSHLRTSSEAHIPHRDTDPAPQPSVTRTPRANREQQDAAGVEQPSQKECGQPA 3605

  Fly  1167 TVEEIPPEIPVSQ----EEIDAEAEA--------------------KRLDSIPTEHDPLPASESH 1207
            ......||:..:|    |:.:||.:|                    ||.:|| ||..|.......
Mouse  3606 GPVAALPEVQATQNPANEQENAEPKAMEEEESGFSSPLMLWLQQEQKRKESI-TERKPKKGLVFE 3669

  Fly  1208 NPGPQDYASCSESSED-----------KASTTSLRKLSKVKKTYLVAGLFSNHYKQSLMPPPAKV 1261
            ......:..|:||.||           ..|...|::||       .||:  |..:...:...|.|
Mouse  3670 ISSDDGFQICAESIEDAWKSLTDKVQEARSNARLKQLS-------FAGV--NGLRMLGILHDAVV 3725

  Fly  1262 NKKPGLEEQVGPASLLPPPPYCEKYLRRTEMDFELPYDIWWAYTNSKLPTRNVVPSWNYRKIRTN 1326
                .|.||:..|.      :|..|..|    |..|.:            .|..|...:...|..
Mouse  3726 ----FLIEQLAGAK------HCRNYKFR----FHKPEE------------ANEPPLNPHGSARAE 3764

  Fly  1327 VYAESVRPNLAGF------DHPTCNCKNQGEKSCLDNCLNRMVYTECSPSNCPAGEKCRN-QKIQ 1384
            |:......::..|      ..|..|..::.|:........|     .:..:.|...:.|: :|..
Mouse  3765 VHLRKSAFDMFNFLASKHRQPPEYNPNDEEEEEVQLKSARR-----ATSMDLPMPMRFRHLKKTS 3824

  Fly  1385 RHAVAPGVERFMTADKGWGVRTKLPIAKGTYILEYVGEVVTEKEFKQRMASIYLNDTHHYCLHLD 1449
            :.||  ||.|  :...|.|:..|..|..|..::||.|.|:...:..:|...........|...:|
Mouse  3825 KEAV--GVYR--SPIHGRGLFCKRNIDAGEMVIEYAGNVIRSIQTDKREKYYDSKGIGCYMFRID 3885

  Fly  1450 GGLVIDGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGEELTYDYNFSLFNPSE 1514
            ...|:|....|:..||:||||||||..:..:::|...:|:||.|.|..||||||||.|.:.:.|.
Mouse  3886 DSEVVDATMHGNAARFINHSCEPNCYSRVINIDGQKHIVIFAMRKIYRGEELTYDYKFPIEDASN 3950

  Fly  1515 GQPCRCNTPQCR 1526
            ..||.|...:||
Mouse  3951 KLPCNCGAKKCR 3962

Known Domains:

Indicated by green bases in alignment.

Software error:

Illegal division by zero at /www/www.flyrnai.org/docroot/cgi-bin/DRSC_prot_align.pl line 591.

For help, please send mail to the webmaster (ritg@hms.harvard.edu), giving this error message and the time and date of the error.