Protein Alignment ash1 and set-16

DIOPT Version :10

• Sequence 1: NP_524160.2
• Gene: ash1 / 40133
• FlyBaseID: FBgn0005386
• Length: 2226
• Species: Drosophila melanogaster
• Sequence 2: NP_499819.3
• Gene: set-16 / 176802
• WormBaseID: WBGene00011729
• Length: 2475
• Species: Caenorhabditis elegans
• Alignment Length: 2109
• Identity: 364/2109 - (17%)
• Similarity: 623/2109 - (29%)
• Gaps: 782/2109 - (37%)

  Fly     3 CSQNETAAAKVL---ETQRAQESGSENEETDSITDQSSQSKSIKSATQFSVQRSDTDGLRMRISA 64
            |.:|.....|::   :..:.|....|...||...:|::..                   |||.||
 Worm   558 CERNYQLNEKLIRCSQCSKWQHGACEGLYTDEQLEQAAID-------------------RMRCSA 603

  Fly    65 IRPTLGVVATKKPPKSRKMSTQDTESGCSEAKNRAVSKKVKVKRKKLASSSGISKSDKVSKSKKS 129
            .||        |..:....|..||......|.::...:.:|.|....|..:.:.:|....:|...
 Worm   604 CRP--------KRVQPSGFSDVDTVWCDYVALDKDAHEILKSKYTPSALKNHMMESVGYRESFDH 660

  Fly   130 QISAFS--SDSEDDL-PLKVHQQRA------------------------PRVLLSAIIQAAQSAS 167
            ....::  .||.|.: ||.:..||.                        ||..:.|:.:.|.:|:
 Worm   661 YDDDYNPLEDSSDQVTPLPIPGQRGRGRGNPSGRRGMNRIGIGGFYAKLPRHRIQALTEEAAAAA 725

  Fly   168 KPTLD---------IGISSSDNELPNLVQAA---IKRVESDT-EDTTVEGSF------------- 206
            ..:.|         ...|..::..|..:|.|   ||.||... .||.|:..|
 Worm   726 ASSEDSKQAKRPRKSRRSQLEDAYPPQIQEAFFGIKAVEGKVLVDTPVDEPFLAEFNGWYKRTRS 790

  Fly   207 RKAAK-------------DKN-------LPQYQSTLLQDFMEKTQMLGQTVNAKLAEEKVAKAKE 251
            .||.:             |:|       ||..::..|.||           :..|.|:.....:.
 Worm   791 TKAFELCMTASEMLRTDIDENEFLDCVGLPNMETDALDDF-----------DFNLLEDFDVDEEN 844

  Fly   252 ETLVQTAVPRKRRGRPKKVVPTVPAPGNSGPAINESADSGV---ISTTSTTQSTTPSPKMQNENA 313
            :.|..:               .:.||||:  .:.|.|..|:   ...||......||.....
 Worm   845 DELENS---------------LLGAPGNT--EMKEDAAEGIEGSYGPTSFNLDQRPSTSSAG--- 889

  Fly   314 VPTGSLPIASSSKPKIDMAYLDKRMYATERVLYPPPRSKRRQNNKKTACSSSNKEELQLDPLWRE 378
               |||.......|.::.|...:..        |..|:...::..:|    .|.|..|....|.|
 Worm   890 ---GSLGSQDPFHPGMNPANFAQHA--------PGVRNAIARSGSQT----DNSERYQFSARWEE 939

  Fly   379 IDVNKKFRLRSMSVGAASGTGASTTICSKVLAA--KSGYVSDYGSVRHQ---------------- 425
            .:.|              |..|:|   :.||.:  |.||      :|.|
 Worm   940 DEPN--------------GLQATT---AAVLYSNEKHGY------LRQQFPNWVDRVKQIQKLWR 981

  Fly   426 RSSHNHNSGYKSDASCKSRYSTKSCMSRRSRAKSCGYRSDCKESGKSGLRMRRKRRASMLLKSSA 490
            ..:|.....|.:.|......|......||:..:|    :|.....:.|.::.....::|:  .|:
 Worm   982 TLNHEVRLDYVNRARDNRTRSGNRPRPRRTNVQS----TDSPPPPQMGFKVPANPSSNMM--GSS 1040

  Fly   491 DDTVEDQDILQLAGLSL--------------------GQSSEESNEYISKPSLKSLPTTSASKKY 535
            :.:::.:.:.....|.:                    |...|:.|:  ::...|:|    |:||.
 Worm  1041 EQSMQPKQVFITHHLPMDVYQVYQDLKRVKFDRERLNGSLEEQLNK--ARKQKKNL----AAKKR 1099

  Fly   536 GEINRYVTTGQYFGRGGSLSATNPDNFISKMMNQRKETPAPSKSSCKIKSRR---SSAASMCSSY 597
            ..:........|.||...|:.::... ::.:..|.|.|.|      :|:..|   ....|..|.:
 Worm  1100 QMVKTQTAAPDYDGRNIDLNESDQQT-LATLTEQIKVTQA------EIELHRRDLKKHESNLSDF 1157

  Fly   598 VSGVSRMRRRHRRKSFSHNKSLNIDSKLLTEIEIITSTFNSRCRIQDDRLTGSSGKEKLLADANK 662
            |.....:|..|..|.              .::::        ..|:|.:....:  :::||...:
 Worm  1158 VMKHGILRNEHEVKR--------------EDVQL--------SAIRDQQYAAHA--DEMLAQQAQ 1198

  Fly   663 LQA-------TLAAPSPA----------------QQLTLNGGGPASTLSK--------------- 689
            .|.       .|.||..|                .|:.:.||.|...:.:
 Worm  1199 NQGLGQVHPPVLVAPQAAISLPMLNQQQQHHQRMMQMQMRGGPPGQFMMRAQPPQQPQINMHNHE 1263

  Fly   690 --------------PLKRGLKKRKLSEPLVD--FAMLSASASGTPNGSGSSNGNTKRRHKKSQSN 738
                          |:..|::..::::|::.  :..|.|.....         :|....:..|:.
 Worm  1264 IAKRMLMQQFRNAHPVMGGIRHDEITDPIMRDVYDCLEAIVFDV---------HTVAEREAMQAA 1319

  Fly   739 DSSSPDDHKLP-LKKRH-------YLLTPGERP----------------------PAEVAF---- 769
            ....|..|.:. :.::|       .|:.||:.|                      |.:.|.
 Worm  1320 GQQPPPPHIMQRMMQQHPGGPGGPRLIMPGQFPQLQGRRIPQQPPPVQLPQVDVDPPKTAAKRKK 1384

  Fly   770 --ANGKLNAEAWAAAA------------------------------------------AAAKSTA 790
              .|.|.||.::.||.                                          .|.::|.
 Worm  1385 GGGNPKKNASSYPAAGEYDAWIEQMRMRFRLCPDIPKKQKEPRLNRPGCEFVSHGLTDMAVRATK 1449

  Fly   791 S-TKSQAQFNARSVKSAL------TPKKRHLLEQPTSVSGAGS-----SASNSPLRIVVDN-NSI 842
            . |....:|.|..||...      ...::.::.|..::.....     :..|:||..:.|: ...
 Worm  1450 KRTPLVGEFGAMRVKRGTRLFGNEERGEKSIMFQDLNLYQVEPQIRLFALYNNPLPTMEDDVFRD 1514

  Fly   843 SGGKLLDISPSSLCSLKQQRRG--GAAKQKVSAAKDLVQLQSPAGSYPPPGVFEPSVELEIQIPL 905
            ...:::|...|:|..|..::||  ||.....|.|:.:.:...|..  |..|:|..:||.|.::.:
 Worm  1515 EEDQIIDKPTSALQKLLNKKRGASGAMDHIRSRARYMEETNIPPA--PERGMFYENVEAEEEVTV 1577

  Fly   906 SKLNESVITKAEVESPLLSALDIKE----DTKKEVGQRVVETLLHKTGGNLLLKR---------- 956
            ..              :.:..||.|    |.||:|..|     ||:...:||..:
 Worm  1578 EL--------------VFNTCDITEESSVDEKKDVCGR-----LHEQIRDLLAIKDEVAWSMEDT 1623

  Fly   957 -KRKKINRTGFP--------------------TVRRKKRKVSVEQQTTAVIDEHEPEFDPDDEPL 1000
             ....::|:..|                    |.|..||:  ||::...|..|.:.|.   .||.
 Worm  1624 PPESPVSRSPEPEATVGAVQQTSSTSRAPSEATEREIKRE--VEEEAAPVAPEIKREI---VEPS 1683

  Fly  1001 QSLRETRSSNNVNVQAAPNPPLDCERVPQAGEARE--TFVARTNQKAPRLSVVALERLQ-RPQTP 1062
            ||...::..|             |::..::..:..  |..|.....||..|||...... .|..|
 Worm  1684 QSQNSSQCKN-------------CQKALESHSSTSIVTVKAEKLNLAPTGSVVTTRAAPVPPGVP 1735

  Fly  1063 -ARGRP-RGRKPKNREQAE-----------AAPQPPPKSEPEIRPAKKRGRQP-----KQPVLEE 1109
             |.|.| ...:.|.:|:..           |:....|.:|.|:..|::...:.     || ||.|
 Worm  1736 GAPGAPIAPSEEKTKEELASFCSKKCYYELASATRAPLTEEEVAAAEQHVSEEVYNKLKQ-VLTE 1799

  Fly  1110 PPPTPPPQQKKNKMEPNIRLPDGIDPNTNFSCKIRLKRRKN--------LEAGTQPKKEKPVQP- 1165
            .......|.|  |.:|.:..|      .:..|.:...||:|        |....:..||.|.|.
 Worm  1800 NIVKAVSQGK--KPDPQLTSP------RDTRCMMDEGRRENVTIVPVASLIGAVEAPKEAPSQRA 1856

  Fly  1166 ------------------VTVEE------IPPEIPVSQEEI--------------DAE-AEAKRL 1191
                              |.:::      :.|:|.|.....              |.| ....||
 Worm  1857 AEAAEDWKAYTKSIWDSFVNIQQQHAHHILSPKIGVPHPPYHLDKRICVFCGGIGDGETTRCGRL 1921

  Fly  1192 DSI--------------------------------------PTEHDPLPAS--ESH--NPGPQDY 1214
            .|:                                      ..:|...|.:  :.|  |.|...:
 Worm  1922 ISLTEFYWVHVNCALWSAEVFENQTGGLTNVDRAVLRAAQTACDHCKRPGASVKCHKMNCGVNYH 1986

  Fly  1215 ASCSESSED---KASTTSLRKLSKVKKTYLVAGL------FSNHYKQSLMPPPAKVNKKPGLEEQ 1270
            ..|:..:..   |..|...::..||....:|..|      :....:.:::....::...|.|..:
 Worm  1987 VLCAMQNNGFFIKDRTFICKQHEKVSNQAIVGRLDALRRIYVKRDENTMLTRLFELTDGPTLCMR 2051

  Fly  1271 VGPASLLPPPPYCEKYLRRTE-MDFELPYD-----IWWAYTNSK--------LPTRNVVP----- 1316
            :|..:.........|.|:|.. .|:..|.:     ::|:..:.:        :..||..|
 Worm  2052 LGAFTFYKIGSITPKQLKRFHTKDYIFPNNYRITRLFWSPKSHRERMMFECIIEDRNNQPIFVVK 2116

  Fly  1317 ----------------SWN--YRKIR---------------TNVYAES-----------VRPNLA 1337
                            :|.  |.|:.               :.:..|:           :..:|.
 Worm  2117 SLEDPTICYKAVSASKAWQPIYEKVHQLREQQQGDSLKFFGSQICGETLFGLNENAITKITESLP 2181

  Fly  1338 GFDHP-TCNCKNQGEKSC-LDNCLNRMVYTECSPSNCPAGEKCR--------------------- 1379
            |||.. |...::|..... |....|.|......|.|...|:..|
 Worm  2182 GFDTIFTYQHRHQNSPVLELPLAENLMGCARAEPRNRTIGQHFRTKPQPMGGTHHHIGSASGLSS 2246

  Fly  1380 ------------NQKIQRHAVAP-----------GVERFMTAD---------------------- 1399
                        |:.:|....|.           ||..:.|.:
 Worm  2247 QATIIASSSSSANESLQSRQAATSVTSANSGRTRGVRSYYTEEAAARARAFGIPPEMASLSLRME 2311

  Fly  1400 ------------------------------KGWGVRTKLPIAKGTYILEYVGEVVTEKEFKQRMA 1434
                                          .|.|:..|:.|:.|.:|:||.||::..:..:.|..
 Worm  2312 TTQNAQTAFSAYQKMRREWKDRVYLARSRIAGLGLYAKVDISMGDFIIEYKGEIIRSEVCEVREI 2376

  Fly  1435 SIYLNDTHHYCLHLDGGLVIDGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSR---MVLFAKRAIE 1496
            .....:...|...:|...|||....|...|::||||:|||..|.......:|   :::.|.|.|.
 Worm  2377 RYVAQNRGVYMFRIDEEWVIDATMAGGPARYINHSCDPNCSTQILDAGSGAREKKIIITANRPIS 2441

  Fly  1497 EGEELTYDYNFSLFNPSEGQPCRCNTPQC 1525
            ..|||||||.|.|...::..||.|..|.|
 Worm  2442 ANEELTYDYQFELEGTTDKIPCLCGAPNC 2470

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ash1	NP_524160.2	PHA03247	<1018..1281	CDD:223021	63/382 (16%)
		AWS	1340..1388	CDD:197795	12/82 (15%)
		SET_ASH1L	1391..1531	CDD:380951	47/190 (25%)
		Bromo_ASH1	1680..1787	CDD:99955
		PHD_ASH1L	1858..1900	CDD:277023
		BAH_polybromo	1929..2073	CDD:240068
set-16	NP_499819.3	PHD_SF	42..84	CDD:276966
		PHD4_KMT2C_like	427..477	CDD:276987
		PHD5_KMT2C_like	479..524	CDD:276988
		PHD6_KMT2C_like	554..604	CDD:276989	12/64 (19%)
		HMG_KMT2C-like	936..1002	CDD:438813	16/88 (18%)
		ePHD2_KMT2C_like	1904..2008	CDD:277136	13/103 (13%)
		FYRN	2053..2104	CDD:461787	7/50 (14%)
		FYRC	2112..2199	CDD:197781	10/86 (12%)
		SET_KMT2C_2D	2319..2474	CDD:380948	44/152 (29%)