Protein Alignment ash1 and set-2

DIOPT Version :10

• Sequence 1: NP_524160.2
• Gene: ash1 / 40133
• FlyBaseID: FBgn0005386
• Length: 2226
• Species: Drosophila melanogaster
• Sequence 2: NP_498039.1
• Gene: set-2 / 175662
• WormBaseID: WBGene00004782
• Length: 1510
• Species: Caenorhabditis elegans
• Alignment Length: 1787
• Identity: 339/1787 - (18%)
• Similarity: 568/1787 - (31%)
• Gaps: 596/1787 - (33%)

  Fly     3 CSQNETAAAKVLETQRAQESGSENEETDSITDQSSQSKSIKSATQFSVQRSDTDGLR-------- 59
            |...|       :.:|.:.:|......:.:.|   ..|:.:|..|.||.:  |:..|
 Worm    59 CKSKE-------DFERIKRTGVRKTSENMLED---PRKNFESLQQSSVYQ--TNSFRNPRYLCRA 111

  Fly    60 -MRISAIRPTLGVVATKKPPKSRKMSTQDTESGCSEAKNRAVSKKV-KVKRKKLASSSGISKSDK 122
             :|:.:...|:       ||| |::|..:.:..|:|...|..:|.. ||::..:.......:..|
 Worm   112 HLRVDSYYCTI-------PPK-REVSLFNMDDNCTEVLLRDFAKDCGKVEKAYVCIHPETKRHMK 168

  Fly   123 VSKSKKSQISAFSSDSE-DDLPLKVHQQR------APRV--LLSAIIQAAQSASK----PTLDIG 174
            ::..|      |::..| .:.....|.|.      .||:  .||.:.:..:.|:.    |.|...
 Worm   169 MAYVK------FATVKEAHNFYSMYHAQNLLATKCTPRIDPFLSILNEEYEVATNGQVLPILPDD 227

  Fly   175 ISSSDNELPNLVQAAIKRVESDTEDTTVEGSFRKAAKDKNLPQYQSTLLQDFMEKTQMLGQTVNA 239
            ::|.|   |::::             .:..:|   .:|:| .:|:..:...:.::..||...:
 Worm   228 LASID---PSVLR-------------DLRANF---LRDQN-EKYELAMRNTYEDEGGMLSGVI-- 270

  Fly   240 KLAEEKVAKAKEETLVQTAVPRKRRGRPKKVVPTVPAPGNSGPAINESADSGVISTTSTTQSTTP 304
                           :.|:...:|                     :.:.|..| ..:|...|..|
 Worm   271 ---------------MDTSDHYER---------------------DYTMDHDV-GPSSMKMSPIP 298

  Fly   305 SPKMQNENAVPTGSLPIASSSK----PKIDMAYLD------KRMYATE-RVLYPPPRSKRRQNNK 358
            .|.::.|:..|....|:||.|.    |.:.:.|.:      ..|:..| |...|||...|....:
 Worm   299 PPPIKEESPPPPPPPPVASVSNLAPVPSVQLPYYNNIQPSSSTMHMPEFRPTEPPPSYSREDPYR 363

  Fly   359 KTACSSSNKEELQLDPLWREIDVNKKFRLRSMSVGA-ASGTGASTTICSKVLAAKSGYVSDYGSV 422
            .|:.||.::.               :.|.||.|.|. .||..:|..
 Worm   364 STSRSSLSRH---------------RNRSRSPSDGMDRSGRSSSRR------------------- 394

  Fly   423 RHQR-SSHNHNSGYKSDASCKSRYSTKSCMSRRSRAKSCGYRSDCKESGKSGLRMRRKRRASMLL 486
            .|:| .|.|.:.....|.   .:|.|.....|:.:.:.         ..|...::..|.|.:::.
 Worm   395 THRRPESRNGSKNANGDV---VKYETYKMEKRKIKYEG---------GNKKYEQVHIKERTAVIR 447

  Fly   487 KSSADDTVEDQDILQLAGLSLGQSSEESNEYISKPSLKSLPTTSASKKYGEINRYVTTGQYFGRG 551
                                            .|..|:::.:.|||                  |
 Worm   448 --------------------------------GKNQLENVSSESAS------------------G 462

  Fly   552 GSLSATNPDNFISKMMNQRKETPAPSKSSCKIKSRRSSAASMCSSYVSGVSRMRRRHRRKSFSHN 616
            .|...|.|| |..:...::|...:|::|     .:.|.|....|:..|.....|||..|   |.|
 Worm   463 SSSVDTYPD-FSDEERKKKKRPKSPNRS-----KKDSRAFGWDSTDESDEDTRRRRSGR---SQN 518

  Fly   617 KSLNIDSKLLTEIEIITSTFNSRCRIQDDRLTGSSGKEKLLADANKLQATLAAPSPAQQLTLNGG 681
            :|                   |..:.|   .|.||...:.|:..:
 Worm   519 RS-------------------SERKFQ---TTSSSSTRRELSSTH-------------------- 541

  Fly   682 GPASTLSKPLKRGLKKRKLSEPLVDFAMLSASASGTPNGSGSSNGNTKRRHKKSQSNDSSSPDDH 746
                |.|.|   .||..:...|            ..|.|..|.:..|..:|.:.|.
 Worm   542 ----TNSVP---NLKSHETPPP------------PPPKGHPSVHLQTPYQHVQPQM--------- 578

  Fly   747 KLPLKKRHYLLTPGERPPAEVAFANGKLNAEAWAAAAAAAKSTASTKSQAQFNARSVKSALTPKK 811
               :...:|.|.|....|..:                     |.|......|:  ......||..
 Worm   579 ---IPATYYNLPPQHMAPPPI---------------------TTSLPPFCDFS--QPPPGFTPTF 617

  Fly   812 RHLLEQPTSVSGAGSSASNSPLRIVVDNNSISGGKLLDISPSSLCSLKQQRRGGAAKQKVSAAKD 876
            :.:...|....   ..|||.|...:|...::|.      :|....|:.....|.|..|     :|
 Worm   618 KPITNAPLPTP---YQASNIPQPGLVQIAALSA------APEPFSSIPGPPPGPAPIQ-----ED 668

  Fly   877 LVQLQSP---------AGSYPPPGVFEPS-VELEIQIPLSKLNES-------------VITKAEV 918
            :.:.:||         :|.:.|....||: ||:|...|| |.:||             |.:..|.
 Worm   669 VGRAESPEKPSLSERFSGIFGPTQREEPAQVEVEYDYPL-KHSESHDDRHSLEDMDVEVSSDGET 732

  Fly   919 ESPLLSALDIKEDTKKEVGQRVV---ETLLHKTGGNLL--LKRK-----RKKINRTGFPTVRRK- 972
            .|. :..::..|:.|::..:|:.   ..::.|....::  |.||     |::|.|..|..:..|
 Worm   733 VSN-VEKIECMEEKKRQDLERIAIARTPIVKKCKKRMMDELSRKVAEDIRQQIMRQCFAALDEKL 796

  Fly   973 ---------KRKVSVEQQTTAVIDEHEPEFDPDDEPLQSLRETR--------------------- 1007
                     |||...|::.....::.......|..|.|:|...:
 Worm   797 HLKAIADEEKRKKEREEKARQEAEKPSNHLIADMMPSQTLYNNQSFASSSRGFYRKQKPIPKSHP 861

  Fly  1008 -------------------SSNNVNVQAAPNP----------------------PLDCERVPQAG 1031
                               ||.:.|...||.|                      ..|.:..|...
 Worm   862 KHQEHHHHAKASVSTPVHSSSTSRNSSVAPTPQRTVSTSSSSSSAATSARVSEDESDSDSTPGEV 926

  Fly  1032 EARETFVARTNQKAPRLSVVALERLQRPQTPARGRPRGRKPKNREQAEAAPQPPPKSEPEIRPAK 1096
            :.|:|.|...:::..|.|..:......|:     |.|.....:|..:.::.....:.|    .|.
 Worm   927 QRRKTSVLSNDKRRRRASFSSTSIQSSPE-----RQRDVSSSSRTSSSSSTSSMKQEE----TAD 982

  Fly  1097 KRGRQPKQPVLEEPPPTPPP------QQKKNKMEPNIRLPDGIDPNTNFSCKIRLKRRKNLEAGT 1155
            ::.|:.|..:..:...|...      ..:::.:||.....||..|..........:|...:|...
 Worm   983 EKSRKRKLIMSSDESSTTGSTATSVVSSRQSSLEPQQEKTDGEPPKKKSQTDFISERVSKIEGEE 1047

  Fly  1156 QPKKEKPVQ---PVTVEE--IPPEIPVSQE-----EIDAEAEAKRLDS---IPTEHDPLPASESH 1207
            :|..| ||:   |:..:.  :|.:| |..|     |::..|.:.|...   ..|||......:..
 Worm  1048 RPLPE-PVETSGPIIGDSSYLPYKI-VHWEKAGIIEMNLPANSIRAHEYHPFTTEHCYFGIDDPR 1110

  Fly  1208 NPGPQ--DYASC-SESSEDKASTT--------------------------SLRKLSKVKKTYLVA 1243
            .|..|  |::.| ||...:....|                          :::|..|.:|.....
 Worm  1111 QPKIQIFDHSPCKSEPGSEPLKITPAPWGPIDNVAETGPLIYMDVVTAPKTVQKKQKPRKQVFEK 1175

  Fly  1244 GLFSNHY-----KQSLMPPPAKVNKKPGLEEQ----VGPASLLPPPPYCEKYLRR--TEMDF--- 1294
            ..: .:|     |:...||..|...||..||:    :|....||... .:.|||.  .||..
 Worm  1176 DPY-EYYEPPPTKRPAPPPRFKKTFKPRSEEEKKKIIGDCEDLPDLE-DQWYLRAALNEMQSEVK 1238

  Fly  1295 ---ELPYDIWWAYTNSKLPTRNVVPSWNYRKIRT----------NVYAESVRPNLAGF------- 1339
               |||   |......|...|:..|......||:          :...:.|.|..||.
 Worm  1239 SADELP---WKKMLTFKEMLRSEDPLLRLNPIRSKKGLPDAFYEDEELDGVIPVAAGCSRARPYE 1300

  Fly  1340 -----------------DHPTCNCKNQGEKSCLD--------NCLNRMVYTECSPSNCPAGEKCR 1379
                             .|||.....:.|.:...        ..|.|.:.|....:|... .|..
 Worm  1301 KMTMKQKRSLVRRPDNESHPTAIFSERDETAIRHQHLASKDMRLLQRRLLTSLGDANNDF-FKIN 1364

  Fly  1380 NQKIQRHAVAPGVERFMTADKGWGVRTKLPIAKGTYILEYVGEVV-------TEKEFKQR-MASI 1436
            ..|.::..:.....|.    .|||:.....||....|:||:|:.:       .||.:::| :.|.
 Worm  1365 QLKFRKKMIKFARSRI----HGWGLYAMESIAPDEMIVEYIGQTIRSLVAEEREKAYERRGIGSS 1425

  Fly  1437 YL--NDTHHYCLHLDGGLVIDGQRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGE 1499
            ||  .|.||         |||..:.|:..||:||||:|||..:..::.|..|:|::::..|::||
 Worm  1426 YLFRIDLHH---------VIDATKRGNFARFINHSCQPNCYAKVLTIEGEKRIVIYSRTIIKKGE 1481

  Fly  1500 ELTYDYNFSLFNPSEGQ--PCRCNTPQCRGVI 1529
            |:||||.|    |.|..  .|.|....|||.:
 Worm  1482 EITYDYKF----PIEDDKIDCLCGAKTCRGYL 1509

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ash1	NP_524160.2	PHA03247	<1018..1281	CDD:223021	62/341 (18%)
		AWS	1340..1388	CDD:197795	10/55 (18%)
		SET_ASH1L	1391..1531	CDD:380951	51/151 (34%)
		Bromo_ASH1	1680..1787	CDD:99955
		PHD_ASH1L	1858..1900	CDD:277023
		BAH_polybromo	1929..2073	CDD:240068
set-2	NP_498039.1	RRM_SF	124..213	CDD:473069	20/95 (21%)
		SET_SETD1	1359..1506	CDD:380946	50/164 (30%)