Protein Alignment ash1 and nsd3

DIOPT Version :10

• Sequence 1: NP_524160.2
• Gene: ash1 / 40133
• FlyBaseID: FBgn0005386
• Length: 2226
• Species: Drosophila melanogaster
• Sequence 2: NP_001362267.1
• Gene: nsd3 / 100216035
• XenbaseID: XB-GENE-5992933
• Length: 1451
• Species: Xenopus tropicalis
• Alignment Length: 1603
• Identity: 342/1603 - (21%)
• Similarity: 526/1603 - (32%)
• Gaps: 497/1603 - (31%)

  Fly   183 PNLVQAAIKRV-ESDTEDTTVEGSFRKAAKDKNLPQYQSTLLQDFM---EKTQMLGQTVNA---K 240
            |.|:.:|..|. ::::.|...||.        ..| |.|.|..||.   ..|:.|....|.   .
 Frog    20 PQLIDSANNRQDDANSSDQADEGG--------GTP-YDSGLQPDFQYPTPTTEDLPPLTNGYQPS 75

  Fly   241 LAEEKVAK-------------------AKEETLVQTAVPRKRRGRPKKVVPTVPAPGNSGPAINE 286
            |..|...|                   |.|...::||..|...|..|...|...:|    |.:.:
 Frog    76 LGYEPQGKYYSQFPNGSANGYGTRNFTALEYYHLETAALRPVEGMSKASPPQPTSP----PTLLQ 136

  Fly   287 SADSGVISTTSTTQSTTPSP--KMQNENAVPTGSLPIASSSKPKIDMAYLDKRMYATERVLYPPP 349
            :..   :||| |.|..|.||  |::....:..|.....||.     ...|....:|:|.......
 Frog   137 APP---LSTT-TPQKKTGSPEIKLRITKTIQNGREMFESSL-----CGDLLHEFHASEMTKKKHE 192

  Fly   350 RSKRRQNNKKTA-------CSSSNKEEL-----------QLDPLWREIDVNKKFRLRSMSVGAAS 396
            |.|.|:..:|.:       ....::||:           :|:|...|..|.|...:..:|....:
 Frog   193 RRKERREKRKKSRHHHHHHHHHHHREEVKAEEQQPPKMKKLEPPPEEERVQKHLPVSPLSPEVRT 257

  Fly   397 GT--GASTTICSKVLAAKSGYVSDYGS-------VRHQRSSHNHNSGYKSDASCKSRYSTKSCMS 452
            |.  .....:.|||           |:       |......|.|:   |.:......|..:...|
 Frog   258 GVWFHVGDLVWSKV-----------GTFPWWPCMVSCDPQLHVHS---KINTRGAREYHVQFFSS 308

  Fly   453 RRSRA-----KSCGYRSD--------------CKESGKSGLRMRRKR-------------RASML 485
            ...||     :...||.:              |.:|.|  |:||:.|             .|...
 Frog   309 EPERAWVHEKRMKEYRGEQQYEQLVAEDSAKACNQSEK--LKMRKPRPQRERVQWEIGIAHAEKA 371

  Fly   486 LKSSADDTVEDQDILQLAGLSLGQSSEESNEYISKP---SLKSLPTTSASKKYGEINRYVTTGQY 547
            |:.:.::.:|....:.:        .||......||   ..||..:||:|:.  |.||.......
 Frog   372 LRMTREERIEQYTFIYV--------DEEKPPEPKKPVNIGKKSRRSTSSSEP--ESNRLSEVLPS 426

  Fly   548 FGRGGS----LSATNPDNFIS-KMMNQRK--------ETPAPSKSSCK-IKSRRSSAASMCSSYV 598
            ..|..|    :|..|.|:... :..:|||        ..|.|:|:..| ..:|:|..||:
 Frog   427 PPRQPSAPSPISHNNKDSPAEHRRQSQRKPPHEEEHPPQPPPAKTPWKTAAARKSLPASV----- 486

  Fly   599 SGVSRMRRRHRRKSFSHNKSLNI---DSKLLTEIEIITSTFNSRCRIQDDRLTGSSGKEKLLAD- 659
                          ..|..:|::   :...:.:||.:.:..|:.          |.|.|:.:..
 Frog   487 --------------LLHKGTLDLQKCNMSPVVKIENVLALQNAT----------SEGFEQFIYTT 527

  Fly   660 ---ANKLQATLA------APSPAQQL------------TLNGGGPAS---------TLSKPLKR- 693
               .||.:.|:.      ||||.|:.            |....||:.         |.|:..|.
 Frog   528 KEVGNKTEITVKAPERAFAPSPNQKSEKSSAQHTPAPETSGATGPSEKKHQRRSNRTRSESEKSL 592

  Fly   694 -GLKKRKLSEPLVDFAMLSASASGTPNGSGSSNGNTKRRHKKSQSNDSSSPDDHKLPLKKRHYLL 757
             .:.|:|:.:..|:...|:|..:|...|:..|    ....|....|.:|...|...|||||
 Frog   593 DSMPKKKVKKEQVEDIPLTAVQTGLQKGASIS----AEPRKIINLNGASEISDACKPLKKR---- 649

  Fly   758 TPGERPPAEVAFANGKLNAEAWAAAAAAAKSTASTKSQAQFNARSVKSALTPKKRHLLEQPTSVS 822
               .|...:|..||....      .|:.:.|.....:|..|                 |:.|...
 Frog   650 ---SRASTDVELANSSYR------DASDSDSRGLNDTQISF-----------------EKQTDSP 688

  Fly   823 GAGSSASNSPLRIVVDNNSISGGKLLDISPSSLCSLKQQRRGGAAKQKVSAAKDLVQLQSPAGSY 887
            .|.:.|..|.::                |..|..|.:..||....:...|..:.||...
 Frog   689 SAAADADASDVQ----------------SVDSSVSHRSSRRDSVCQICESYGESLVSCD------ 731

  Fly   888 PPPGVFEPSVELEIQIPLSKLNESVITKAEVESPLLSALDIKEDTKKEVGQRVVETLLHKTGGNL 952
               |.......||. :.:..|.:......|.::.|.:....|          :..|
 Frog   732 ---GECSRLFHLEC-LGMDTLPDGKFICMECKTGLHTCFSCK----------IPST--------- 773

  Fly   953 LLKRKRKKINRTGFPTVRRKKRKVSVEQQTTAVIDE------HE--PEFDPDDEPLQSLRETRSS 1009
                   .:.|...|...:...:....|.:..:.|.      |.  .....|.:|
 Frog   774 -------SVKRCSAPCCGKFYHETCARQYSATIFDSKGFRCPHHCCSSCTADKDP---------- 821

  Fly  1010 NNVNVQAAPNPPLDCERVPQAGEARETFVARTNQKAPRLSVVALERLQRPQTPARGRPRG----- 1069
                .:|.....|.|.|.|.|....::.:|..:.......::.....:|....:.....|
 Frog   822 ----YRACKGRMLSCVRCPVAYHNGDSCIAAGSLHLSSSLIICSNHSKRSGHASSSVNVGFCFVC 882

  Fly  1070 ----------------------RKPKNREQAEAAPQPPPKSEPEIRPAKKRGRQPKQPVLEEPPP 1112
                                  .....|.:....|..|..|...|:.::|.|  .|....:..|.
 Frog   883 ARGLIVQDYSLVSSMSFKSHFLLTDSKRAELMKIPMIPSSSPAYIKKSEKGG--GKLLCCDSCPA 945

  Fly  1113 TPPPQQKKNKMEPNIRLPDGIDPNTNFSCKIRLKRRKNLEAGTQPKKEKPVQPVTVE-------- 1169
            ...|...      |:.:|.|     ::||       .:..:|   ||....|.|.|:
 Frog   946 AFHPSCL------NLEMPQG-----SWSC-------NDCRSG---KKLHYKQIVWVKLGNYRWWP 989

  Fly  1170 -EI--PPEIPVSQEEIDAEAEAKRLDSIPTEHD----PLPASESHN-------------PGPQDY 1214
             ||  |..:|::.:.:              :||    |:....||:             .|.:.:
 Frog   990 AEICNPRSVPLNIQGL--------------KHDIGDFPVFFFGSHDYYWVHQGRVFPYVEGDKSF 1040

  Fly  1215 ASCSES---SEDKASTTSLRKLSKVKKTYLVAGLFSNHYKQSLMPPPAKVNKKPGLEEQVGPASL 1276
            |....|   :..||...:.|:..::|                     |:...|..||  :...|.
 Frog  1041 ADGQTSINKTFKKALEEAARRFQELK---------------------AQRESKEALE--IEQNSR 1082

  Fly  1277 LPPPPYCEKYLRRTEMDFELPYDIWWAYTNSKLPTRNVVPSWNYRKIRTNVYAESVRPNLAGFDH 1341
            .|||                                       |:.|::|.....|:..||....
 Frog  1083 KPPP---------------------------------------YKHIKSNKPVGKVQIQLADLSE 1108

  Fly  1342 -PTCNCKNQGEKSC-LD-NCLNRMVYTECSPSNCPAGEKCRNQKIQRHAVAPGVERFMTADKGWG 1403
             |.|||:...:..| || .|||||:..||.|..|||||.|.||...:. :.|..|.|:|..:|||
 Frog  1109 IPRCNCRPTDDTPCGLDTECLNRMLLYECHPQICPAGEHCLNQNFTKR-LYPEAEIFITERRGWG 1172

  Fly  1404 VRTKLPIAKGTYILEYVGEVVTEKEFKQRMASIYLN-DTHHYCLHLDGGLVIDGQRMGSDCRFVN 1467
            :|||..|.||.::.|||||::.|:|.:|||...:.| .|:.|.|.:....:||....|:..||:|
 Frog  1173 LRTKRDIRKGEFVNEYVGELIDEEECRQRMKRAHENGTTNFYLLTVTKDRIIDAGPKGNYSRFMN 1237

  Fly  1468 HSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGEELTYDYNFSLFNPSEGQPCRCNTPQCRGVIGGK 1532
            |||.||||.|||:|||..|:.|||...|.:|.|||::||........ :.|.|....|.|.:|
 Frog  1238 HSCNPNCETQKWTVNGDVRVGLFALCDIPKGAELTFNYNLDCLGNGR-RECHCGAENCSGFLG-- 1299

  Fly  1533 SQRVKPLPAVEAKPSGEGLSGRNGRQRKQKAK-KHAQRQAGKD 1574
               |:|..|. |..:.|  ..:|.|.:.:|.| |..|:|..:|
 Frog  1300 ---VRPKTAC-ATATEE--RSKNARLKAKKRKLKTEQKQMHED 1336

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
ash1	NP_524160.2	PHA03247	<1018..1281	CDD:223021	52/320 (16%)
		AWS	1340..1388	CDD:197795	23/50 (46%)
		SET_ASH1L	1391..1531	CDD:380951	60/140 (43%)
		Bromo_ASH1	1680..1787	CDD:99955
		PHD_ASH1L	1858..1900	CDD:277023
		BAH_polybromo	1929..2073	CDD:240068
nsd3	NP_001362267.1	PWWP_NSD3_rpt1	261..389	CDD:438991	24/151 (16%)
		PHD1_NSD3	717..759	CDD:277119	8/51 (16%)
		PHD_SF	764..810	CDD:473978	6/71 (8%)
		PHD_SF	811..863	CDD:473978	9/65 (14%)
		PHD_SF	934..966	CDD:473978	9/51 (18%)
		PWWP_NSD3_rpt2	973..1067	CDD:438994	19/128 (15%)
		AWS	1118..1156	CDD:465559	19/37 (51%)
		SET_NSD3	1158..1299	CDD:380989	61/141 (43%)
		PHD5_NSD3	1337..1379	CDD:277131	342/1603 (21%)
		C5HCH	1378..1427	CDD:465605