DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment ash1 and nsd2

DIOPT Version :9

Sequence 1:	NP_001246834.1	Gene:	ash1 / 40133	FlyBaseID:	FBgn0005386	Length:	2226	Species:	Drosophila melanogaster
Sequence 2:	NP_001076020.1	Gene:	nsd2 / 100000709	ZFINID:	ZDB-GENE-030131-2581	Length:	1461	Species:	Danio rerio

Alignment Length:	1692	Identity:	361/1692 - (21%)
Similarity:	547/1692 - (32%)	Gaps:	480/1692 - (28%)

- Green bases have known domain annotations that are detailed below.

  Fly   185 LVQAAIKRVESDTEDTTVE--GSFRKAAKDKNLPQYQSTLLQDFMEKTQMLGQTVNAKLAEEKVA 247
            |:..|..::.:..:::..:  ||.......::|....|.||....||...|    .|..|.:.:.
Zfish    44 LMNKAATQLAATPQESVPQMIGSHGHNHTHEHLKDLTSRLLNGDTEKIPKL----CAAPAGQPLL 104

  Fly   248 KAKEETLVQTAVPRKRRGRPKKVVPTVPAPGNSGPAIN-----------ESADS------GVIST 295
            |..|......:.|||....|:..|.......|.|...|           |..|:      ||:::
Zfish   105 KGVEPVSQHASPPRKTSVSPELTVKITKCVVNLGGKPNRYEVDYTEEKAEETDAPLRLPEGVLTS 169

  Fly   296 TSTTQSTTPSPKMQNENAVPTGSLPIASSSKPKIDMAYLDKRMYATERVLYP---PPRSKRRQNN 357
            .:..:.......|..:...|...|..|:||.   ....|...:.:.:..:.|   |..|....:.
Zfish   170 PTARRDRQKKKGMVRKRIPPYPILTHAASSD---QSPLLHPTINSNDITIEPCGLPVESIEHTHG 231

  Fly   358 KKTACSSSNKEELQL---------------DPLWREIDVNKKFRLRSMSVGAASGTGASTTICSK 407
            ....|.::..:|..|               |....|.||.:.:.....:|..:.|    ..|.:|
Zfish   232 DMPTCVTAFPDEQPLSDCEETVDAQGVDETDDAQGENDVKESYTETQSNVHFSVG----DVIWTK 292

  Fly   408 VLAAKSGY-------VSDYGSVRHQRSSHNHNSG------YKSDA--------------SCKSRY 445
            |    |||       .:|.....|.||..|..:|      |..|.              |.|.:|
Zfish   293 V----SGYPWWPCMITTDPEFNMHFRSKVNSRTGLLYHVQYFGDTPERGYVFEKCIVTFSGKHQY 353

  Fly   446 STKS-------CMSRRSRAKSCGYRSDCK---ESGKSGLRMRRKRRASMLLKSSADDTVEDQDIL 500
            .:.|       .:|.|.||....:|...:   ...|..|.:..:.|   |.|.|   .|.:....
Zfish   354 QSLSRSNQLPINISGRKRAVPRKFRQKWEIGVSQAKEALGLLTEER---LAKFS---FVYEDGKA 412

  Fly   501 QLAGLSLGQSSEESNEYISKPSLKSLPTTSASKKYGEINRYVTTGQYFGRGGSLSATNPDNFISK 565
            ||....|....|:.|:.:.:..::..|.        |:               :|.|.||:..|.
Zfish   413 QLNPHMLQVLEEQRNQGLQEEPVQREPQ--------EL---------------VSLTPPDSTHSS 454

  Fly   566 MMNQRKETPAPSKSSCKIKSRRSSAASMCSSYVSGVSRMRRRHRRKSFSHNKSLNIDSKLLTEIE 630
            ..|....:..||..|..|.|..|          ||.:.:.|:.|:.|...|:.....|    .||
Zfish   455 PTNSSTTSTIPSLFSNPIDSSTS----------SGKTALPRKARKPSVKTNRRKQRSS----TIE 505

  Fly   631 IITSTFNSRCRIQDDRLTGSSGKEKLLADANKLQATLAAPSPAQQ-------LTLNGGGPASTLS 688
            .|.:..:|..::.   ||.|:.:.::..:..|...|.|...|.::       |.|.   |.::..
Zfish   506 WIVTEASSAKQMP---LTESTVQCEVNTEHKKKGKTNAGKRPYRKKDLLCIDLDLQ---PKASQK 564

  Fly   689 KP---------LKRGLKKRKLSEPLVDFAMLSASASGTPNGSGSSNGNTKRRHKKSQSNDSSSPD 744
            ||         ....:||||:.        ...|...|.|...:|.|.::.:        :|:|
Zfish   565 KPRHIRQKFPAAPNSVKKRKIK--------AGVSICDTLNTVSTSAGLSEVK--------ASAP- 612

  Fly   745 DHKLPLKKRHYLLTPGERPPAEVAFANGKLNAEAWAAAAAAAKSTASTKSQAQFNARSVKS---- 805
                                          :.:.|      .|..||:|...:...||.:|
Zfish   613 ------------------------------STKLW------EKEDASSKGMKKVCKRSRESKQDV 641

  Fly   806 ALTPKKRHLLEQPTSVSGAGSSASNSPLRIVV----------DNNSISGGKLLDISPSSLCSLKQ 860
            .|.|||:..||:.........:||::|..:..          |.|.....|.....|.......:
Zfish   642 TLQPKKKSKLEKDFKKWKCPKAASSAPAAVPAPRGRRRKTDRDENRKRSKKASKEEPPPKAKRGR 706

  Fly   861 QRRGGAAKQKVSAAKDLVQLQSPAGSYPPPGVFEPSVELEIQIPLSKLNESVITKAEVESPLLSA 925
            :|:..:.:|...|:|..    .|:....|.....||...:...|..|...:  ..|:.||..|..
Zfish   707 KRKEESGRQSFPASKKC----RPSSYAEPERPDSPSDSTDESHPSKKAERT--PGAKKESVCLVC 765

  Fly   926 LDIKEDTKKEVGQRVVETLLHKTGGNLLLKRKRKKINRTGFPT--------------VR------ 970
            ....||.....||......||..|    ::|..:||..|...|              ||
Zfish   766 EQTGEDIVTCAGQCYGTYHLHCIG----VERSAEKILCTACSTGVHVCFTCKKSEGEVRRCCALH 826

  Fly   971 --RKKRKVSVEQQTTAVIDEHEPEFDPDDEPLQSLRETRSSNNVNVQAAPNPPLDCERVPQAGEA 1033
              |...:..|......|.:......     ||.:......|.....:|.....:.|.|.|.|...
Zfish   827 CGRFYHEACVRLSALTVFENRGFRC-----PLHTCLSCHYSGRAACKATKGKMMRCLRCPVAYHV 886

  Fly  1034 RETFVARTNQKAPRLSVVALERLQRPQTPARGRPRGRKPKNREQAEAAPQPPPKSEPEIR---PA 1095
            .:..||..::.....::|.....           |.:|..:..           |...:.   ..
Zfish   887 GDLCVAAGSEMISSSAIVCTNHF-----------RAKKGYSHH-----------SHVNVSWCFIC 929

  Fly  1096 KKRGRQPKQPVL--EEPPPTPPPQQKKNKMEPNIRLPDGIDPNTNFSCKIRLKRRKNLEAGTQPK 1158
            .|.||     :|  |..|....|...      ||.:|||     ::.|       .:..:|.:||
Zfish   930 SKGGR-----LLCCESCPAAFHPDCL------NIAMPDG-----SWFC-------NDCRSGKKPK 971

  Fly  1159 KEKPV---------QPVTVEEIPPEIPVSQEEIDAEAEAKRLDSIPTEHDPLPASESHNP----G 1210
            ....:         .|                    ||.:...:|||....|.......|    |
Zfish   972 YRDVIWVKLGNYRWWP--------------------AEIRHPKNIPTNIQHLRHEIGEFPVFFFG 1016

  Fly  1211 PQDYASCSES------SEDKASTTSLRKLSKVKKTYLVAGLFSNHYKQSLMPPPAKVNKKPGLEE 1269
            .:||....:.      ..|:.|......:.||             :|.:|:.
Zfish  1017 SKDYFWTHQGRVFPYMEGDRGSKYQQTGIGKV-------------FKNALLD------------- 1055

  Fly  1270 QVGPASLLPPPPYCEKYLRRTEMDFELPYDIWWAYTNSKLPTRNVVPSWNYRKI-----RTNVYA 1329
                         .|...:..||:.|...    |:.|:|.|     |.:.|.|:     |..||.
Zfish  1056 -------------AETRFKEIEMEREAKE----AHENNKKP-----PPFKYIKVNKPCGRVQVYT 1098

  Fly  1330 ESVRPNLAGFDHPTCNCKNQGEKSCL--DNCLNRMVYTECSPSNCPAGEKCRNQKIQRHAVAPGV 1392
            ..:.      :.|.||||...|:.|.  ..|||||:..||.|..|||||:|:||...:. :.|..
Zfish  1099 ADIS------EIPKCNCKPSTERPCSFESECLNRMLLYECHPQVCPAGERCQNQDFTKR-LYPET 1156

  Fly  1393 ERFMTADKGWGVRTKLPIAKGTYILEYVGEVVTEKEFKQRMASIYLND-THHYCLHLDGGLVIDG 1456
            :...||.||||:.:...|.||.::.|||||::.|:|.:.|:.....|| ||.|.|.:|...:||.
Zfish  1157 KIIRTAGKGWGLISLRDIKKGEFVNEYVGELIDEEECRSRIRHAQENDITHFYMLTIDKDRIIDA 1221

  Fly  1457 QRMGSDCRFVNHSCEPNCEMQKWSVNGLSRMVLFAKRAIEEGEELTYDYNFSLFNPSEGQPCRCN 1521
            ...|:..||:||||:||||.|||:|||.:|:.|||...|..|.|||::||..... :|...|||.
Zfish  1222 GPKGNYSRFMNHSCQPNCETQKWTVNGDTRVGLFAVCDIPAGTELTFNYNLDCLG-NEKTVCRCG 1285

  Fly  1522 TPQCRGVIGGKSQRVKPLPAVEAKPSGEGLSGRNGRQRKQKAKKHAQRQAGK------------- 1573
            .|.|.|.:|.             :|.....|....:.:|:|.|:...|..||
Zfish  1286 APNCSGFLGD-------------RPKNGHTSEPKAKLQKKKPKRKRARNEGKKSEDECFRCGDGG 1337

  Fly  1574 -----DISSAVAVAKLQPLSEKEKKLVR----------------QFNTFLVRNFEKIRRCKAKRA 1617
                 |.........|..|...::...|                .|......:|     |||.:
Zfish  1338 QLVLCDKKGCTKAYHLSCLDRTKRPFGRWDCPWHHCDVCGKNSAAFCQLCPNSF-----CKAHQ- 1396

  Fly  1618 SDAAATASSPALGT------TNGDIPGRRPSTPSSPSLAAQISALCSPRNIKTRGLTQAVHDPEL 1676
              ..|..|.|..|.      .:.||   ||.|.:     ||:.....||..:.|...:......|
Zfish  1397 --EGALRSHPLTGQLCCQEHEDSDI---RPQTET-----AQLPPPTKPRKYRRRAEAKTTSKAPL 1451

  Fly  1677 EK 1678
            :|
Zfish  1452 KK 1453

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
ash1	NP_001246834.1	AWS	1340..1388	CDD:197795	23/49 (47%)
		SET	1392..1512	CDD:214614	54/120 (45%)
		Bromo_ASH1	1680..1787	CDD:99955
		PHD_ASH1L	1858..1900	CDD:277023
		BAH_polybromo	1929..2073	CDD:240068
nsd2	NP_001076020.1	PWWP	283..394	CDD:295359	26/118 (22%)
		DTHCT	688..>758	CDD:285332	13/75 (17%)
		PHD_SF	761..803	CDD:304600	12/45 (27%)
		PHD_SF	808..854	CDD:304600	6/50 (12%)
		PHD_SF	855..908	CDD:304600	9/52 (17%)
		PHD4_NSD2	925..965	CDD:277127	13/62 (21%)
		WHSC1_related	970..1063	CDD:99899	20/151 (13%)
		AWS	1103..1153	CDD:197795	23/50 (46%)
		SET	1154..1277	CDD:214614	55/123 (45%)
		PHD_SF	1329..1371	CDD:304600	4/41 (10%)
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170593888
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	1	1.010	-	-		D507784at2759
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4489
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	4	3.880