DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment Shal and KCND2

DIOPT Version :9

Sequence 1:	NP_001097646.1	Gene:	Shal / 40129	FlyBaseID:	FBgn0005564	Length:	571	Species:	Drosophila melanogaster
Sequence 2:	NP_036413.1	Gene:	KCND2 / 3751	HGNCID:	6238	Length:	630	Species:	Homo sapiens

Alignment Length:	538	Identity:	373/538 - (69%)
Similarity:	436/538 - (81%)	Gaps:	14/538 - (2%)

- Green bases have known domain annotations that are detailed below.

  Fly     2 ASVAAWLPFARAAAIGWVPIATHPLPPPPMPKDRRKTDDEKLLINVSGRRFETWRNTLEKYPDTL 66
            |.|||||||||||||||:|:|:.|:|.||. ::|::|.|..:::||||.||:||::|||:|||||
Human     3 AGVAAWLPFARAAAIGWMPVASGPMPAPPR-QERKRTQDALIVLNVSGTRFQTWQDTLERYPDTL 66

  Fly    67 LGSNEREFFYDEDCKEYFFDRDPDIFRHILNYYRTGKLHYPKHECLTSYDEELAFFGIMPDVIGD 131
            |||:||:|||..:.::|||||||||||||||:|||||||||:|||:::|||||||||::|::|||
Human    67 LGSSERDFFYHPETQQYFFDRDPDIFRHILNFYRTGKLHYPRHECISAYDEELAFFGLIPEIIGD 131

  Fly   132 CCYEDYRDRKRENAERLMDDKLSENGDQNLQQLTNMRQKMWRAFENPHTSTSALVFYYVTGFFIA 196
            ||||:|:||:|||||||.||..::...::.......||::|||||||||||.|||||||||||||
Human   132 CCYEEYKDRRRENAERLQDDADTDTAGESALPTMTARQRVWRAFENPHTSTMALVFYYVTGFFIA 196

  Fly   197 VSVMANVVETVPCGHRPGRAGTLPCGERYKIVFFCLDTACVMIFTAEYLLRLFAAPDRCKFVRSV 261
            |||:||||||||||..||....|||||||.:.|||||||||||||.||||||.|||.|.:|||||
Human   197 VSVIANVVETVPCGSSPGHIKELPCGERYAVAFFCLDTACVMIFTVEYLLRLAAAPSRYRFVRSV 261

  Fly   262 MSIIDVVAIMPYYIGLGITDNDDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGF 326
            |||||||||:||||||.:|||:|||||||||||||||||||||||||||||||||||||||||||
Human   262 MSIIDVVAILPYYIGLVMTDNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGF 326

  Fly   327 LVFSLAMAIIIFATVMFYAEKNVNGTNFTSIPAAFWYTIVTMTTLGYGDMVPETIAGKIVGGVCS 391
            |:|||.|||||||||||||||..:.:.|||||||||||||||||||||||||:||||||.|.:||
Human   327 LLFSLTMAIIIFATVMFYAEKGSSASKFTSIPAAFWYTIVTMTTLGYGDMVPKTIAGKIFGSICS 391

  Fly   392 LSGVLVIALPVPVIVSNFSRIYHQNQRADKRKAQRKARLARIRIAKASSGAAFVSKKKAAEARWA 456
            |||||||||||||||||||||||||||||||:||:||||||||.||:.|..|::..|:.......
Human   392 LSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKKARLARIRAAKSGSANAYMQSKRNGLLSNQ 456

  Fly   457 AQESGIELDDNYRDEDIFELQHHHLLRCLEKTTDREFVELEIPFN------GQPKRPGSPSPMAS 515
            .|.|..|.....:....||.||||||.||||||:.|||:.:: |.      ....||.|.||
Human   457 LQSSEDEQAFVSKSGSSFETQHHHLLHCLEKTTNHEFVDEQV-FEESCMEVATVNRPSSHSP--- 517

  Fly   516 PAHSTNSAAGLLQSCCGR 533
               |.:|..|:..:||.|
Human   518 ---SLSSQQGVTSTCCSR 532

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Shal	NP_001097646.1	BTB_POZ_Shal-like	6..144	CDD:349727	94/137 (69%)
		Ion_trans	188..415	CDD:395416	195/226 (86%)
		DUF3399	445..540	CDD:403174	33/95 (35%)
KCND2	NP_036413.1	Interaction with KCNIP1, KCNIP2, and other family members. /evidence=ECO:0000250\|UniProtKB:Q63881	2..20		15/16 (94%)
		Shal-type	3..28	CDD:288455	19/24 (79%)
		BTB_2	43..132	CDD:308049	63/88 (72%)
		Interaction with KCNIP1. /evidence=ECO:0000250\|UniProtKB:Q63881	71..90		11/18 (61%)
		Ion_trans	184..415	CDD:306908	199/230 (87%)
		S4-S5 linker. /evidence=ECO:0000250\|UniProtKB:P63142	308..321		12/12 (100%)
		Selectivity filter. /evidence=ECO:0000250\|UniProtKB:P63142	370..375		4/4 (100%)
		DUF3399	445..546	CDD:314709	33/95 (35%)
		Important for normal channel activation and inactivation, for interaction with KCNIP2, and probably other family members as well. /evidence=ECO:0000250\|UniProtKB:Q63881, ECO:0000305\|PubMed:16934482	474..630		29/66 (44%)
		Required for dendritic targeting. /evidence=ECO:0000250\|UniProtKB:Q63881	474..489		12/14 (86%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	600..630
		PDZ-binding. /evidence=ECO:0000250\|UniProtKB:Q63881	627..630
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	402	1.000	Domainoid score	I731
eggNOG	1	0.900	-	-		E1_COG2126
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H40828
Inparanoid	1	1.050	757	1.000	Inparanoid score	I557
Isobase	1	0.950	-	0	Normalized mean entropy	S2308
OMA	1	1.010	-	-		QHG48930
OrthoDB	1	1.010	-	-		D221939at33208
OrthoFinder	1	1.000	-	-		FOG0001867
OrthoInspector	1	1.000	-	-		otm41431
orthoMCL	1	0.900	-	-		OOG6_103639
Panther	1	1.100	-	-	LDO	PTHR11537
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R4179
SonicParanoid	1	1.000	-	-		X19
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	16	15.820