Protein Alignment not and USP4

DIOPT Version :10

• Sequence 1: NP_001401041.1
• Gene: not / 40030
• FlyBaseID: FBgn0013717
• Length: 703
• Species: Drosophila melanogaster
• Sequence 2: NP_003354.2
• Gene: USP4 / 7375
• HGNCID: 12627
• Length: 963
• Species: Homo sapiens
• Alignment Length: 639
• Identity: 119/639 - (18%)
• Similarity: 184/639 - (28%)
• Gaps: 310/639 - (48%)

  Fly   363 GLRGLLNLGATCFMNCIVQALVHTPLLSDYFMSDRHDC--------GSKSSHKCLVCEV-SRLFQ 418
            ||.||.|||.|||||..:|.|.:|..|:|||:.|.::.        |.|..    :.|. :.|.:
Human   300 GLCGLGNLGNTCFMNSALQCLSNTAPLTDYFLKDEYEAEINRDNPLGMKGE----IAEAYAELIK 360

  Fly   419 EFYSGSRSPLSLHRLLHLIWNHAKHLAGYEQQDAHEFFIATLDVLHR--HCVKAKAEHESKSNSS 481
            :.:||..:.::.......:...|...:||:|||:.|.....||.||.  :.||.|...|.| :::
Human   361 QMWSGRDAHVAPRMFKTQVGRFAPQFSGYQQQDSQELLAFLLDGLHEDLNRVKKKPYLELK-DAN 424

  Fly   482 GSGSGTNSSNSSSSHCYGQCNCIIDQIFTGMLQSDVVCQACNGVSTTYDPFWDISL--------- 537
            |......:..:..:|.....:.|:| .|.|:.:|.:||..|..||.|:|||..::|
Human   425 GRPDAVVAKEAWENHRLRNDSVIVD-TFHGLFKSTLVCPECAKVSVTFDPFCYLTLPLPLKKDRV 488

  Fly   538 ----------------------------------------------------------------- 537
                                                                             
Human   489 MEVFLVPADPHCRPTQYRVTVPLMGAVSDLCEALSRLSGIAAENMVVADVYNHRFHKIFQMDEGL 553

  Fly   538 ----------------------------------------------------------------- 537
                                                                             
Human   554 NHIMPRDDIFVYEVCSTSVDGSECVTLPVYFRERKSRPSSTSSASALYGQPLLLSVPKHKLTLES 618

  Fly   538 ----------------------------------------------------------------- 537
                                                                             
Human   619 LYQAVCDRISRYVKQPLPDEFGSSPLEPGACNGSRNSCEGEDEEEMEHQEEGKEQLSETEGSGED 683

  Fly   538 ----DLGETT---THGGVTPKTLI----------------------------------------- 554
                |..|||   ..|...||.|.
Human   684 EPGNDPSETTQKKIKGQPCPKRLFTFSLVNSYGTADINSLAADGKLLKLNSRSTLAMDWDSETRR 748

  Fly   555 -------------------------------DCLERYTRAEHLGSAAKIKCSTCKSYQESTKQFS 588
                                           ||:|.:|..|.||......|..||.:|::||:|.
Human   749 LYYDEQESEAYEKHVSMLQPQKKKKTTVALRDCIELFTTMETLGEHDPWYCPNCKKHQQATKKFD 813

  Fly   589 LRTLPSVVSFHLKRFEHSALIDRKISSFIQFPVE-FDMTPF---MSEKKNAYGDFRFSLYAVVNH 649
            |.:||.::..|||||.::.....|:.:.::||:. .:|:.|   :|.:...|     .|.||.||
Human   814 LWSLPKILVVHLKRFSYNRYWRDKLDTVVEFPIRGLNMSEFVCNLSARPYVY-----DLIAVSNH 873

  Fly   650 VGTIDTGHYTAYVRHQ-KDTWVKCDDHVITMASLKQVLDSEGYLLFYHKNVLEY 702
            .|.:..||||||.::: ...|...||..:::||..|::....|:|||.:...|:
Human   874 YGAMGVGHYTAYAKNKLNGKWYYFDDSNVSLASEDQIVTKAAYVLFYQRRDDEF 927

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
not	NP_001401041.1	zf-UBP	253..310	CDD:460464
		Peptidase_C19D	365..696	CDD:239125	115/629 (18%)
USP4	NP_003354.2	Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	1..22
		Necessary for interaction with SART3. /evidence=ECO:0000269|PubMed:20595234	27..216
		UBP12	35..923	CDD:227847	118/633 (19%)
		Nuclear export signal. /evidence=ECO:0000250|UniProtKB:P35123	133..141
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	219..253
		Required for USP4 activation by providing conformational flexibility between the DUSP and catalytic domains. /evidence=ECO:0000269|PubMed:25404403	229..295
		Regulates ubiquitin dissociation. /evidence=ECO:0000269|PubMed:25404403	384..386		0/1 (0%)
		Necessary for interaction with RBL2. /evidence=ECO:0000250|UniProtKB:P35123	405..407		1/1 (100%)
		Necessary for interaction with RB1 and RBL2. /evidence=ECO:0000250|UniProtKB:P35123, ECO:0000269|PubMed:11571652	459..463		2/3 (67%)
		Interacts with DUSP and ubiquitin-like 1 domains and is required for USP4 activation. /evidence=ECO:0000269|PubMed:25404403	485..775		8/289 (3%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	637..699		4/61 (7%)
		Nuclear localization signal. /evidence=ECO:0000250|UniProtKB:P35123	767..772		0/4 (0%)
		Disordered. /evidence=ECO:0000256|SAM:MobiDB-lite	928..963		119/639 (19%)