Protein Alignment Nedd4 and ube3a

DIOPT Version :10

• Sequence 1: NP_648993.1
• Gene: Nedd4 / 39958
• FlyBaseID: FBgn0259174
• Length: 1007
• Species: Drosophila melanogaster
• Sequence 2: NP_001007319.1
• Gene: ube3a / 792398
• ZFINID: ZDB-GENE-041114-190
• Length: 857
• Species: Danio rerio
• Alignment Length: 1022
• Identity: 243/1022 - (23%)
• Similarity: 382/1022 - (37%)
• Gaps: 286/1022 - (27%)

  Fly    79 GQSLAKKDIFGASDPYVRIDLNTINGDINIDSV-LTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQ 142
            |......:..|:|..:.|:|    |....|.:: |.|...|..:|          .||: |....
Zfish    22 GNEACTNEACGSSPGFQRMD----NNAAAIKALELYKNNAKLCDP----------HPSK-KGASS 71

  Fly   143 VFDENRL---------------------TRDDFLGMVELT------LVNLPTEQE---------G 171
            .|.||..                     ||:||..:..||      ::::.:|.|         |
Zfish    72 AFPENSAKGAHNFSACSNGKMNHKDLPPTREDFRDLNFLTEDKVYEILSICSETEDYSPLIRVIG 136

  Fly   172 RTIGEQSYTLRPRRSVGAKSRIKGTLRIYHAFIRETRE-QSEPSSGNSDG-EWEHVEATNAGETS 234
            |........::..|.  ||...|..|:...|...:..| :.|.:||:|.. |.|...:.::|:.|
Zfish   137 RVFSSAESLVQSFRK--AKQHTKEELKSLQAKDEDKDEDEKETASGSSTAMEVEPEASASSGDGS 199

  Fly   235 AQPHPFPTGGHDALPAGWEERQ---DANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQLASD- 295
            :.      |.::....|..|..   ||..|.|                          |:|.|:
Zfish   200 SH------GENNVQKLGPVEVSVDIDAVRRVY--------------------------DRLLSNE 232

  Fly   296 -FQRRFHISVDDTESGRSADSISHN-------------SIEDNNNAAGLAY----TPKTAATSSA 342
             .:..|..::.........|...||             .:.:|:|.....|    .|:.....|
Zfish   233 KIEAAFLNALVYLSPNVECDLTYHNVYAADPNYLNVFIIVMENSNLHSPEYLEIALPQFCKAMS- 296

  Fly   343 PPNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRP---V 404
              ..|......||::..||..::               :|..|...|..|:.|.:.|:...   |
Zfish   297 --KLPLPALAKLARLWSQYGVDQ---------------IRRLVETFQQLITYTVISNEFSSDNLV 344

  Fly   405 REAPGVPDIAITNPFTRRAAGNMAGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQ 469
            .|..||  :|.|.........|:.||.                               ||.||
Zfish   345 NEDDGV--VAATKCLKIVYYANVLGGD-------------------------------LDTDH-- 374

  Fly   470 QEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAP----STRRNSEEDNAAVPPMEQNTG----- 525
                             ||:.|.......|:::..    ..|||.:  ...|.|:|...|
Zfish   375 -----------------NEEDDDEPIPESSELTLQELLGEERRNKK--GPRVDPLETEMGIRASD 420

  Fly   526 -------GEE---EPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQTRRVEDDLG 580
                   .||   |||.....|.   ...|||......:.:::        ..|.....|..:||
Zfish   421 CRKPLIPFEEFINEPLNEVLEMD---KDYTFFKVETESKFSFM--------TCPFILNAVTKNLG 474

  Fly   581 PLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLS--NPNIAGQAV-PYSRDYKQKYEYFKSHIRK 642
                            .|.|:..|  .:.:.|::  ...:.||.: ||.|
Zfish   475 ----------------LYYDNRIR--MYSERRITALYSLVQGQQLNPYLR--------------- 506

  Fly   643 PTNVPNKFEIRIRRTSILEDS---YRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLS 704
                     :::||..|::|:   ..:|:.....| ||.:|:||||||.|:|.||:::|:|.|:.
Zfish   507 ---------LKVRRDHIIDDALVRLEMIAMENPAD-LKKQLYVEFEGEQGVDEGGVSKEFFQLVV 561

  Fly   705 KEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYK 769
            :|:|||..|:|.|.  ::..|...|.|...||   ..|..||.:.|:|:|:..:||..|....|:
Zfish   562 EEIFNPDIGMFTYD--ESTKLFWFNPSSFENE---GQFTLIGIVLGLAIYNNCILDVHFPMVIYR 621

  Fly   770 MMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILE---LTFCLDE-DVFGQKSQHELKPGGANID 830
            .::.|....:|:.......:.||..:.|.:..:.|   :||.:.: |:||....::||..|..|.
Zfish   622 KLMGKKGTFRDLADSHPVLFQSLKDLMEYEGNVEEDMMITFQISQTDLFGNPLMYDLKESGDKIP 686

  Fly   831 VTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIK-IFDEHELELLMCGIQNIDVKD 894
            |||||:.:::.|..|:.....|::|..:|..||..:...:.:| :|...|:|||:||.:|:|.:.
Zfish   687 VTNENRKDFVALYAEYMLNKSVEKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQA 751

  Fly   895 WRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNG---FKELYGSNGPQM 956
            ..|:|.|.|.|:.:..||:.||..|.||..|.:...|||.|||.|.|:.|   .|.:...|||.
Zfish   752 LEESTEYDGGYNKDSRIIRDFWETVHSFEQEKKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPD- 815

  Fly   957 FTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGF 1003
                    .:..|.:|||||.|.||.|....:||::|:|||..::||
Zfish   816 --------SDRLPTSHTCFNVLLLPEYSTVEKLKERLLKAITYAKGF 854

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	33/161 (20%)
		WW	248..277	CDD:459800	6/31 (19%)
		HUL4	<523..1003	CDD:227354	146/508 (29%)
ube3a	NP_001007319.1	AZUL	6..60	CDD:465173	10/41 (24%)
		HECTc	505..855	CDD:238033	126/389 (32%)