Protein Alignment Nedd4 and itch

DIOPT Version :10

• Sequence 1: NP_648993.1
• Gene: Nedd4 / 39958
• FlyBaseID: FBgn0259174
• Length: 1007
• Species: Drosophila melanogaster
• Sequence 2: NP_001072737.1
• Gene: itch / 780194
• XenbaseID: XB-GENE-976485
• Length: 853
• Species: Xenopus tropicalis
• Alignment Length: 1062
• Identity: 344/1062 - (32%)
• Similarity: 467/1062 - (43%)
• Gaps: 323/1062 - (30%)

  Fly    51 GNGYTPRRSLAAVNDSGDSCHLRIVVLTGQ-SLAKKDIFGASDPYVRIDLNTINGDINIDSVLTK 114
            |:|..|    .|||.......|:::|::.: ...:|:.||.| |||.:   :::|    .|..|:
 Frog     3 GSGSRP----GAVNGFTMKSQLQVIVISAKLKDNRKNWFGPS-PYVEL---SVDG----QSKKTE 55

  Fly   115 TKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTL---------------VN 164
            ||.||.:|.|.:.....|.|.. .|.|:|:....|..|..:|...|.|               |.
 Frog    56 TKNKTHSPKWKQPLTVIVTPFS-VLNFRVWSHQTLKSDALIGSASLNLHETLLSNNMKLEEVIVT 119

  Fly   165 LPTEQEGRTIGEQSYTL-------------RPRRSVGAKSRIKGTLRIYHAFI-----------R 205
            :....:..|:|:.|..|             .|..|.|:.......:....|..           |
 Frog   120 MQLLSDRETVGDLSVCLDGMQVDPELLANGDPAVSSGSTDNSSTEVESQAASAESSNGTNSTDSR 184

  Fly   206 ETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFP-------TGGHDA---------------- 247
            .|...|.||..|..     .:|:.....|..|.|.|       |...:|
 Frog   185 TTNGMSSPSLANGG-----FKASRPPRPSRPPPPTPRRASGVVTAISEAPPPPEAELLPTPATAS 244

  Fly   248 ----------------------------------LPAGWEERQDANGRTYYVNHTARTTQWDRPT 278
                                              ||.|||:|.|..||.|||:|.|:.|.||||
 Frog   245 LPEAQAPSSTPPVSRQIAPINVDGQRMGLVNQGPLPPGWEQRVDQQGRVYYVDHVAKRTTWDRP- 308

  Fly   279 VLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAP 343
                       :.|...::||    ||                    |...:.|
 Frog   309 -----------EPLPPGWERR----VD--------------------NMGRIYY----------- 327

  Fly   344 PNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRPVREAP 408
                                       |||
 Frog   328 ---------------------------VDH----------------------------------- 330

  Fly   409 GVPDIAITNPFTRRAAGNMAGGAGWQQ---ERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQ 470
                  ||...|            ||:   |..|...|..:|::|.:...||.|:..:   :..|
 Frog   331 ------ITRTTT------------WQRPTLESVRNYEQWQLQRNQLQGAMQQFNQRFI---YGTQ 374

  Fly   471 EPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRW 535
            |                                ..|.:|.|.|     |:        .|||..|
 Frog   375 E--------------------------------TGTTQNKEYD-----PL--------GPLPVGW 394

  Fly   536 SMQVAPNGRTFFIDHASRRTTWIDPRN-GRASPMPNQTRRVEDDLGPLPEGWEERVHTDGRVFYI 599
            ..::..|||.:|::|.:|.|.|.|||| |:.:..            |||||||.|...||..:::
 Frog   395 EKRMDGNGRVYFVNHTTRTTQWEDPRNQGQLNEK------------PLPEGWEMRFTVDGIPYFV 447

  Fly   600 DHNTRTTQWEDPR-----LSN-PNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTS 658
            |||.:||.:.|||     |.| |:||     |.||:|.|.:||:...:: ..:|...:|.:.|.:
 Frog   448 DHNRKTTTYIDPRTGKSALDNGPHIA-----YVRDFKAKVQYFRFWCQQ-LYMPQHVKITVNRKT 506

  Fly   659 ILEDSYRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNY 723
            :.|||::.|.|....| |:.:||:...||.||||||:|||||:|||.|:.||.|.||||:..|||
 Frog   507 LFEDSFQQIMSFNAQD-LRRRLWIIIPGEEGLDYGGVAREWFFLLSHEVMNPMYCLFEYAGKDNY 570

  Fly   724 TLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEY 788
            .||||..|.: |.:||.||:||||...||::|||.:|..|..||||.:|.||:.|||:||||.|:
 Frog   571 CLQINPASYI-NPDHLRYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESVDPEF 634

  Fly   789 YNSLMWIKENDPRI--LELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVAR 851
            ||||:|||:|:...  ||:.|.:|:::.|....|:|||.|:||.||.|||:|||:||.|||....
 Frog   635 YNSLIWIKDNNIEECGLEMFFSVDKEILGDVKSHDLKPDGSNIQVTEENKEEYIRLVAEWRLSRG 699

  Fly   852 VKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFW 916
            |:||..:|.:||..|:|...::.||..|||:|:||:|.||:.||:.||:|: .|......|.|||
 Frog   700 VEEQTQAFFEGFNEILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRNTIYR-HYTRTSKQIIWFW 763

  Fly   917 RAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLP 981
            :.|....||.|.|||||||||.|:|:.||.:|.||||||.|.|||.|..|..||:||||||||||
 Frog   764 QFVKEIDNEKRMRLLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLP 828

  Fly   982 PYEGYLQLKDKLIKAIEGSQGF 1003
            ||:.|.|||:||:.|||.::||
 Frog   829 PYKSYEQLKEKLLFAIEETEGF 850

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	37/161 (23%)
		WW	248..277	CDD:459800	17/28 (61%)
		HUL4	<523..1003	CDD:227354	237/488 (49%)
itch	NP_001072737.1	C2_E3_ubiquitin_ligase	17..137	CDD:175988	32/128 (25%)
		WW	279..308	CDD:459800	17/28 (61%)
		HUL4	<310..853	CDD:227354	269/725 (37%)