DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nedd4 and smurf1

DIOPT Version :9

Sequence 1:	NP_648993.1	Gene:	Nedd4 / 39958	FlyBaseID:	FBgn0259174	Length:	1007	Species:	Drosophila melanogaster
Sequence 2:	NP_001001943.1	Gene:	smurf1 / 321695	ZFINID:	ZDB-GENE-040426-2744	Length:	731	Species:	Danio rerio

Alignment Length:	984	Identity:	304/984 - (30%)
Similarity:	417/984 - (42%)	Gaps:	308/984 - (31%)

- Green bases have known domain annotations that are detailed below.

  Fly    66 SGDSCHLRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIF 130
            :|.|..:|:.||..::|||||.|...||:.::   .::|.....|  |.|.|.||:|.||:.:..
Zfish     9 NGSSIKIRLTVLCAKNLAKKDFFRLPDPFAKV---VVDGSGQCHS--TDTVKSTLDPKWNQHYDL 68

  Fly   131 RVKPSEHKLVFQVFDENRLTRDD---FLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRSVGAKSR 192
            .:..:: .:...|::..::.:..   |||.|.| |.|                        |.||
Zfish    69 YIGKTD-SITISVWNHKKIHKKQGAGFLGCVRL-LSN------------------------AISR 107

  Fly   193 IKGTLRIYHAFIRETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFPTGGHDALPAGWEERQD 257
            :|.|                                               |:..|..
Zfish   108 LKDT-----------------------------------------------GYQRLDL------- 118

  Fly   258 ANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIE 322
                                ..||...|.:...|:....|.|     |...||
Zfish   119 --------------------CKLNPSDSDAVRGQIVVSLQTR-----DRIGSG------------ 146

  Fly   323 DNNNAAGLAYTPKTAATSSAPPNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAH 387
                                                         .|.||....|.|.
Zfish   147 ---------------------------------------------GPVVDCRGLVENE------- 159

  Fly   388 RQPEISATSLQNDLRPVRE--APGVP-------DIAITNPFTRRAAGNMAGGAGWQQERRRQQMQ 443
                          .||.|  .||.|       .:..|:|......||........||:|.|..:
Zfish   160 --------------GPVFEDSGPGRPLSCFMEEPMPYTDPTGAAGGGNCRALESPNQEQRLQAQR 210

  Fly   444 LHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRR 508
            :                       |.|:  .||..|..|:||.
Zfish   211 I-----------------------RNQD--SRGHTHTPQNRPH---------------------- 228

  Fly   509 NSEEDNAAVPPMEQNTGGEEEP-LPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQT 572
                             |.:.| ||..:..:....|:.:|:...:..:||.|||      :|...
Zfish   229 -----------------GHQSPDLPEGYEQRTTVQGQVYFLHTQTGVSTWHDPR------IPRDL 270

  Fly   573 RRVE-DDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRL-------SNPNIAGQAVP----- 624
            ..|. ::|||||.|.|.|....||::::|||.||||:.||||       |....:.||:|
Zfish   271 NSVSCEELGPLPPGREIRSTVSGRIYFVDHNNRTTQFTDPRLHHIMSQHSQVKESSQALPVQMEV 335

  Fly   625 ------------YSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSVTKTDLLK 677
                        |.||..||.:..:..:...........|.:.|..|.|:|||.|..:...| ||
Zfish   336 GMEEGDGELPVRYERDLVQKLKVLRHELSLQQPQAGHCRIEVSREEIFEESYRQIMKMRPKD-LK 399

  Fly   678 TKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYF 742
            .:|.|:|.||.||||||:||||.|||..||.|||||||:||..:.||||||..|.: |.:|||||
Zfish   400 KRLMVKFRGEEGLDYGGVAREWLYLLCHEMLNPYYGLFQYSTDNIYTLQINPDSSI-NPDHLSYF 463

  Fly   743 KFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKEND-PRILELT 806
            .|:|||.|:||:||..::..|..||||.:|.|||.|.|:|:||.|.:.||:||.||| ..:|:.|
Zfish   464 HFVGRIMGLAVFHGHYINGGFTLPFYKQLLGKPIQLCDLETVDPELHKSLVWILENDITSVLDHT 528

  Fly   807 FCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNL 871
            ||::.:.||:..||||||.|.||.||.|||.||::|.:.|||:..::.|..:...||..:||.:|
Zfish   529 FCVEHNAFGKFLQHELKPNGKNIPVTEENKKEYVRLYVNWRFMRGIEAQFLALQKGFNELIPQHL 593

  Fly   872 IKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYH--MNHIIIQWFWRAVLSFSNEMRSRLLQFV 934
            :|.||..||||::.|:..||:.||:.||..|   |  .:..|::|||:||.||..|.|.||||||
Zfish   594 LKPFDNKELELIIGGLGKIDLNDWKANTRLK---HCVADSNIVKWFWQAVESFDEERRGRLLQFV 655

  Fly   935 TGTSRVPMNGFKELYGSN---GPQMFTIEKW-GTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIK 995
            ||::|||:.|||.|.||.   ||::|||... ...:|.|:|||||||:|:||||.|.:|.:||:.
Zfish   656 TGSTRVPLQGFKALQGSTGSAGPRLFTIHLIDANTDNLPKAHTCFNRIDIPPYESYEKLYEKLLT 720

  Fly   996 AIEGSQGFA 1004
            |:|.:.|||
Zfish   721 AVEETCGFA 729

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	34/135 (25%)
		WW	248..277	CDD:278809	1/28 (4%)
		WW	531..560	CDD:278809	6/28 (21%)
		WW	581..613	CDD:197736	17/31 (55%)
		HECTc	650..1003	CDD:238033	184/359 (51%)
		HECTc	674..1003	CDD:214523	176/335 (53%)
smurf1	NP_001001943.1	C2_Smurf-like	14..138	CDD:176028	40/228 (18%)
		WW	234..266	CDD:197736	9/37 (24%)
		WW	280..312	CDD:197736	17/31 (55%)
		HECTc	373..728	CDD:238033	184/359 (51%)
		HECTc	397..728	CDD:214523	176/335 (53%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2273
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
ZFIN	0	0.000	Not matched by this tool.
	5	4.740