DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nedd4 and Itch

DIOPT Version :9

Sequence 1:	NP_648993.1	Gene:	Nedd4 / 39958	FlyBaseID:	FBgn0259174	Length:	1007	Species:	Drosophila melanogaster
Sequence 2:	XP_008760557.1	Gene:	Itch / 311567	RGDID:	1359556	Length:	864	Species:	Rattus norvegicus

Alignment Length:	1049	Identity:	337/1049 - (32%)
Similarity:	463/1049 - (44%)	Gaps:	324/1049 - (30%)

- Green bases have known domain annotations that are detailed below.

  Fly    72 LRIVVLTGQ-SLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKK--KTLNPTWNEEFIFRVK 133
            |:|.|::.: ...||:.||.| |||         ::.:|....||:|  .|.:|.|.:.....|.
  Rat    20 LQITVISAKLKENKKNWFGPS-PYV---------EVTVDGQSKKTEKCNNTNSPKWKQPLTVIVT 74

  Fly   134 PSEHKLVFQVFDENRLTRDDFLGM------------------VELTLVNLPTEQEGRTIGEQSYT 180
            |.. ||.|:|:....|..|..||.                  |.:||..:..::...|:|:.|..
  Rat    75 PMS-KLCFRVWSHQTLKSDVLLGTAGLDIYETLKSNNMKLEEVVMTLQLVGDKEPTETMGDLSVC 138

  Fly   181 L----------------------------RPR---------------RSVGAKSRIKG--TLRIY 200
            |                            ||:               .:.|...|..|  :..:.
  Rat   139 LDGLQVEAEVVTNGETSCSEGATQNDAGCRPKDETRVSTNGSEDPEVAASGENKRANGNNSPSLS 203

  Fly   201 HAFIRETR--EQSEP--------------SSGNSDGEWEHVEA-----TNAGETSAQ-------- 236
            :...:.:|  ..|.|              .|.|||.:.....:     ||...::::
  Rat   204 NGGFKPSRPPRPSRPPPPTPRRPASVNGSPSTNSDSDGSSTGSLPPTNTNVNSSASEGAASGLII 268

  Fly   237 ---------PHPFPTGGHDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQL 292
                     |.|..|.....||.|||:|.|.:||.|||:|..:.|.||||            :.|
  Rat   269 PLTISGGSGPRPLNTVSQAPLPPGWEQRVDQHGRVYYVDHVEKRTTWDRP------------EPL 321

  Fly   293 ASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTPTNNNGILAQI 357
            ...::||    ||                    |...:.|
  Rat   322 PPGWERR----VD--------------------NMGRIYY------------------------- 337

  Fly   358 AMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRPVREAPGVPDIAITNPFTRR 422
                         |||                                             |||.
  Rat   338 -------------VDH---------------------------------------------FTRT 344

  Fly   423 AAGNMAGGAGWQQE-----RRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQ 482
            ..        ||:.     |..:|.||...|.|...||..|..|.
  Rat   345 TT--------WQRPTLESVRNYEQWQLQRSQLQGAMQQFNQRFIF-------------------- 381

  Fly   483 HRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFF 547
               .|:|..             :|.:|.|.|     |:        .||||.|..:...|||.:|
  Rat   382 ---GNQDLF-------------ATSQNKEFD-----PL--------GPLPPGWEKRTDSNGRVYF 417

  Fly   548 IDHASRRTTWIDPRN-GRASPMPNQTRRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDP 611
            ::|.:|.|.|.|||: |:.:..            |||||||.|...||..:::|||.|.|.:.||
  Rat   418 VNHNTRITQWEDPRSQGQLNEK------------PLPEGWEMRFTVDGIPYFVDHNRRATTYIDP 470

  Fly   612 R-----LSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSVT 671
            |     |.|    |..:.|.||:|.|.:||:...:: ..:|...:|.:.|.::.|||::.|.|.:
  Rat   471 RTGKSALDN----GPQIAYVRDFKAKVQYFRFWCQQ-LAMPQHIKITVTRKTLFEDSFQQIMSFS 530

  Fly   672 KTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNE 736
            ..| |:.:|||.|.||.||||||:|||||:|||.|:.||.|.||||:..|||.||||..|.: |.
  Rat   531 PQD-LRRRLWVIFPGEEGLDYGGVAREWFFLLSHEVLNPMYCLFEYAGKDNYCLQINPASYI-NP 593

  Fly   737 EHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPR 801
            :||.||:||||...||::|||.:|..|..||||.:|.||:.|||:||:|.|:||||:|:|||:..
  Rat   594 DHLKYFRFIGRFIAMALFHGKFIDTGFSLPFYKRILNKPVGLKDLESIDPEFYNSLIWVKENNIE 658

  Fly   802 I--LELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFG 864
            .  ||:.|.:|:::.|:...|:|||.|.||.||.|||:|||::|.|||....|:||..:|.:||.
  Rat   659 ECGLEMYFSVDKEILGEIKSHDLKPNGGNILVTEENKEEYIRMVAEWRLSRGVEEQTQAFFEGFN 723

  Fly   865 SIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSR 929
            .|:|...::.||..|||:|:||:|.||:.||:.:.:|: .|......|.|||:.|....||.|.|
  Rat   724 EILPQQYLQYFDAKELEVLLCGMQEIDLNDWQRHAIYR-HYTRTSKQIVWFWQFVKEIDNEKRMR 787

  Fly   930 LLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLI 994
            ||||||||.|:|:.||.:|.||||||.|.|||.|..|..||:||||||||||||:.|.|||:||:
  Rat   788 LLQFVTGTCRLPVGGFADLMGSNGPQKFCIEKVGKENWLPRSHTCFNRLDLPPYKSYEQLKEKLL 852

  Fly   995 KAIEGSQGF 1003
            .|||.::||
  Rat   853 FAIEETEGF 861

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	40/197 (20%)
		WW	248..277	CDD:278809	16/28 (57%)
		WW	531..560	CDD:278809	12/28 (43%)
		WW	581..613	CDD:197736	18/36 (50%)
		HECTc	650..1003	CDD:238033	187/354 (53%)
		HECTc	674..1003	CDD:214523	180/330 (55%)
Itch	XP_008760557.1	C2_E3_ubiquitin_ligase	17..140	CDD:175988	34/130 (26%)
		WW	289..318	CDD:278809	16/28 (57%)
		WW	321..350	CDD:278809	15/143 (10%)
		WW	401..430	CDD:278809	12/28 (43%)
		WW	440..471	CDD:197736	16/30 (53%)
		HECTc	509..862	CDD:238033	189/356 (53%)
		HECTc	533..861	CDD:214523	180/330 (55%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	0	0.000	Not matched by this tool.
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	4	3.810