DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Nedd4 and pub1

DIOPT Version :9

Sequence 1:	NP_648993.1	Gene:	Nedd4 / 39958	FlyBaseID:	FBgn0259174	Length:	1007	Species:	Drosophila melanogaster
Sequence 2:	NP_594396.1	Gene:	pub1 / 2541818	PomBaseID:	SPAC11G7.02	Length:	767	Species:	Schizosaccharomyces pombe

Alignment Length:	982	Identity:	336/982 - (34%)
Similarity:	476/982 - (48%)	Gaps:	259/982 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly    63 VNDSGDSCHLRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEE 127
            :::|..|..:|:.::....|.|:|:|...||:..:   |::|:   .:..|...||||||.|||.
pombe     1 MSNSAQSRRIRVTIVAADGLYKRDVFRFPDPFAVL---TVDGE---QTHTTTAIKKTLNPYWNET 59

  Fly   128 FIFRVKPSEHKLVFQVFDENRLTR--DDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRS---- 186
            |...|..:. .:..||||:.:..:  ..|||::.|.:.::.....|   |::..|...::|
pombe    60 FEVNVTDNS-TIAIQVFDQKKFKKKGQGFLGVINLRVGDVLDLAIG---GDEMLTRDLKKSNENT 120

  Fly   187 -------VGAKSRIKGTLRIYHAFIRETREQ-----SEPSSGNSDGEWEHVEATNAGE------- 232
                   :...:..:.||::..:.....|.|     ::|.|..|.....:..::.||.
pombe   121 VVHGKIIINLSTTAQSTLQVPSSAASGARTQRTSITNDPQSSQSSSVSRNPASSRAGSPTRDNAP 185

  Fly   233 ----TSAQPHPFPT--GGHDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQ 291
                .|::|..|.:  ..:..||.|||.|.|..||||||:|..|:|.|.||.:
pombe   186 AASPASSEPRTFSSFEDQYGRLPPGWERRTDNLGRTYYVDHNTRSTTWIRPNL------------ 238

  Fly   292 LASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTPTNNNGILAQ 356
                                             ::.||.|.....::.|||
pombe   239 ---------------------------------SSVAGAAAAELHSSASSA-------------- 256

  Fly   357 IAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRPVREAPGVPDIAITNPFTR 421

pombe   257 ----------------------------------------------------------------- 256

  Fly   422 RAAGNMAGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQHRPS 486
                |:..|.
pombe   257 ----NVTEGV------------------------------------------------------- 262

  Fly   487 NEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFFIDHA 551
                            .||:...:....|:|......|.|..| |||.|..:..|.||.:|:||.
pombe   263 ----------------QPSSSNAARRTEASVLTSNATTAGSGE-LPPGWEQRYTPEGRPYFVDHN 310

  Fly   552 SRRTTWIDPRNG---RASPMPNQT---RRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWED 610
            :|.|||:|||..   |:...||..   ::....|||||.|||.|:....||:::||||:||.|:|
pombe   311 TRTTTWVDPRRQQYIRSYGGPNNATIQQQPVSQLGPLPSGWEMRLTNTARVYFVDHNTKTTTWDD 375

  Fly   611 PRLSNPNIAGQAVP-YSRDYKQKYEYFKSHIRKPT--NVPNKFEIRIRRTSILEDSYRIISSVTK 672
            |||  |:...|.|| |.||:::|..||.|   :|.  .:|.:..|::||..|.||||..|...:.
pombe   376 PRL--PSSLDQNVPQYKRDFRRKLIYFLS---QPALHPLPGQCHIKVRRNHIFEDSYAEIMRQSA 435

  Fly   673 TDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEE 737
            || ||.:|.::|:||.|||||||:||:|:|||.|||||:|.|||||::||||||||..||: |.|
pombe   436 TD-LKKRLMIKFDGEDGLDYGGLSREYFFLLSHEMFNPFYCLFEYSSVDNYTLQINPHSGI-NPE 498

  Fly   738 HLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKEND-PR 801
            ||:|||||||:.|:|::|.:.:||||:..||||:|||.:.|:||||:|.|||.||:||.:|| ..
pombe   499 HLNYFKFIGRVIGLAIFHRRFVDAFFVVSFYKMILQKKVTLQDMESMDAEYYRSLVWILDNDITG 563

  Fly   802 ILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSI 866
            :|:|||.::::.||:....:|||.|.||:||.|||.||:.||..||...|::||.::|.:||..:
pombe   564 VLDLTFSVEDNCFGEVVTIDLKPNGRNIEVTEENKREYVDLVTVWRIQKRIEEQFNAFHEGFSEL 628

  Fly   867 IPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLL 931
            ||..||.:|||.|||||:.||..||::||:::|.|: .|..|..||:|||..:..:|||.:||||
pombe   629 IPQELINVFDERELELLIGGISEIDMEDWKKHTDYR-SYSENDQIIKWFWELMDEWSNEKKSRLL 692

  Fly   932 QFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKA 996
            ||.|||||:|:||||:|.||:||:.|||||.|.||..|:|||||||||||||.....|..||..|
pombe   693 QFTTGTSRIPVNGFKDLQGSDGPRKFTIEKAGEPNKLPKAHTCFNRLDLPPYTSKKDLDHKLSIA 757

  Fly   997 IEGSQGF 1003
            :|.:.||
pombe   758 VEETIGF 764

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Nedd4	NP_648993.1	C2_NEDD4_NEDD4L	71..204	CDD:175999	35/145 (24%)
		WW	248..277	CDD:278809	17/28 (61%)
		WW	531..560	CDD:278809	14/28 (50%)
		WW	581..613	CDD:197736	18/31 (58%)
		HECTc	650..1003	CDD:238033	198/353 (56%)
		HECTc	674..1003	CDD:214523	188/329 (57%)
pub1	NP_594396.1	HUL4	1..767	CDD:227354	336/982 (34%)
		C2_Smurf-like	9..131	CDD:176028	33/131 (25%)
		WW	207..236	CDD:278809	17/28 (61%)
		WW	290..319	CDD:278809	14/28 (50%)
		WW	346..378	CDD:197736	18/31 (58%)
		HECTc	413..765	CDD:238033	200/355 (56%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Domainoid	1	1.000	359	1.000	Domainoid score	I158
eggNOG	1	0.900	-	-		E1_COG5021
Hieranoid	0	0.000	Not matched by this tool.
Homologene	1	1.000	-	-		H86986
Inparanoid	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0000080
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	1	0.900	-	-		OOG6_100256
Panther	1	1.100	-	-	O	PTHR11254
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R2273
SonicParanoid	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
	9	8.800