DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment U4-U6-60K and prpf4

DIOPT Version :9

Sequence 1:	NP_648990.1	Gene:	U4-U6-60K / 39955	FlyBaseID:	FBgn0036733	Length:	553	Species:	Drosophila melanogaster
Sequence 2:	NP_956049.1	Gene:	prpf4 / 326944	ZFINID:	ZDB-GENE-030131-5143	Length:	507	Species:	Danio rerio

Alignment Length:	550	Identity:	280/550 - (50%)
Similarity:	361/550 - (65%)	Gaps:	47/550 - (8%)

- Green bases have known domain annotations that are detailed below.

  Fly     1 MSDDDDIQYIKRQRTLHYGSLEESERKRQNAAASGAAATTTSGTTASSGAGTTTTGTGGQLEDID 65
            |||:|:...:||.| :.||||||.|::|  .:..|.:.|......|...||.....:|..:|
Zfish     1 MSDEDEAPVVKRSR-IFYGSLEEKEKER--LSREGTSTTAKDAVKAGIEAGHINISSGESME--- 59

  Fly    66 SDEDYEESTKKTSNAKQAGAPPPTAATLANKIDDDYFDLEMEMERDKVALLEEFERKKRARQINV 130
                                                  :|..:...:..:|.||||:||||||.|
Zfish    60 --------------------------------------MEERVSERQAEVLAEFERRKRARQITV 86

  Fly   131 STDDTEIKSNLRQLNEPICYFGEGPAERRRRLKELLAGLGENAINKRQYEDEERKQQQREQDQAT 195
            ||||.|:|:.||.|.|||..|||||||||.||:.:|:.||.:|:.|.:.|||:.|:...|..| |
Zfish    87 STDDVEVKACLRALGEPITLFGEGPAERRERLRNVLSVLGPDALKKSKKEDEKAKRTHEEYQQ-T 150

  Fly   196 WYHEGPDSLRIARLWLADYSLPRAKDRLVRAREALEVPSAARAGRMVEMQKKLQSLAPLCSQVGD 260
            ||||||.||:.||||||.||||||..||..||...|:|.:.|..:..|:.|.|:::...|||:||
Zfish   151 WYHEGPTSLKEARLWLAKYSLPRAVKRLDAARAQKEIPESTRTIKQQELHKNLRNVNNFCSQIGD 215

  Fly   261 TRPVSSAAFSEDSSLLLTSSWSGLCKLWSVPDCELKQTLRGHASYVGGVALRPGVKAD-EENVVA 324
            .||:|...||.:|.||:|:|||||||||.||||.|.:|||||.:.||.::..|..... ||:.|.
Zfish   216 DRPISYCQFSPNSKLLVTASWSGLCKLWKVPDCTLIRTLRGHNTNVGAISFHPQATLTLEESDVN 280

  Fly   325 MASGGHDGAVKLWGFNNEESIADITGHMPHRVSKVAFHPSGRFLATACYDSSWRLWDLEQKTEVL 389
            |||...||:||||..:::|.||||.|| ..||::|.:|||||||.|.|||.||||||||.:.|:|
Zfish   281 MASCAADGSVKLWSLDSDEPIADIEGH-SMRVARVTWHPSGRFLGTTCYDHSWRLWDLEVQEEIL 344

  Fly   390 HQEGHAKPVHCLSYHSDGSVLVTGGLDAFGRVWDLRTGRCIMFLEGHLGAVFGVDFSPNGFHIAT 454
            |||||:|.||.|.:|.|||:..|||||:|.||||||||||:||||||:..::.::|||||||:||
Zfish   345 HQEGHSKGVHDLHFHPDGSLAGTGGLDSFARVWDLRTGRCVMFLEGHIKEIYSINFSPNGFHVAT 409

  Fly   455 GSQDNTCKIWDLRRRQPVYTIPAHTNLISDVKYQQECGSFLVTCSYDSTTKIWSNKTWQPLKTLQ 519
            ||.||.||:||||:|:.:||||||.||:|.||:|...|.:|:|.:||:|.|||::..|.|||||.
Zfish   410 GSGDNACKVWDLRQRRCIYTIPAHQNLVSSVKFQPNDGHYLLTGAYDNTAKIWTHPGWSPLKTLA 474

  Fly   520 GHDNKVISVDIAPNSQYIATTSFDRTFKLW 549
            ||:.||:.||::|:.|.|||.|:|||||||
Zfish   475 GHEGKVMGVDLSPDGQLIATCSYDRTFKLW 504

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
U4-U6-60K	NP_648990.1	SFM	132..>166	CDD:128776	22/33 (67%)
		WD40 repeat	264..301	CDD:293791	23/36 (64%)
		WD40	<286..553	CDD:225201	159/264 (60%)
		WD40	286..550	CDD:238121	159/264 (60%)
		WD40 repeat	357..393	CDD:293791	24/35 (69%)
		WD40 repeat	398..434	CDD:293791	25/35 (71%)
		WD40 repeat	441..476	CDD:293791	22/34 (65%)
		WD40 repeat	482..519	CDD:293791	18/36 (50%)
		WD40 repeat	525..551	CDD:293791	15/24 (63%)
prpf4	NP_956049.1	SFM	88..124	CDD:128776	23/35 (66%)
		WD40	<192..504	CDD:225201	182/312 (58%)
		WD40	218..504	CDD:238121	172/286 (60%)
		WD40 repeat	219..256	CDD:293791	23/36 (64%)
		WD40 repeat	262..306	CDD:293791	19/43 (44%)
		WD40 repeat	311..347	CDD:293791	24/35 (69%)
		WD40 repeat	354..389	CDD:293791	24/34 (71%)
		WD40 repeat	395..429	CDD:293791	20/33 (61%)
		WD40 repeat	437..474	CDD:293791	18/36 (50%)
		WD40 repeat	480..504	CDD:293791	14/23 (61%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	1	0.930	-	-		C170580202
Domainoid	1	1.000	94	1.000	Domainoid score	I7481
eggNOG	1	0.900	-	-		E1_KOG0272
Hieranoid	1	1.000	-	-
Homologene	1	1.000	-	-		H3446
Inparanoid	1	1.050	553	1.000	Inparanoid score	I1092
OMA	1	1.010	-	-		QHG54420
OrthoDB	1	1.010	-	-		D1076587at2759
OrthoFinder	1	1.000	-	-		FOG0004342
OrthoInspector	1	1.000	-	-		oto39748
orthoMCL	1	0.900	-	-		OOG6_102752
Panther	1	1.100	-	-	LDO	PTHR19846
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R1883
SonicParanoid	1	1.000	-	-		X3609
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
ZFIN	0	0.000	Not matched by this tool.
	16	15.800