DRSC - DRSC Integrative Ortholog Prediction Tool

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Protein Alignment CG12229 and PKLR

DIOPT Version :9

Sequence 1:	NP_648980.1	Gene:	CG12229 / 39945	FlyBaseID:	FBgn0036723	Length:	571	Species:	Drosophila melanogaster
Sequence 2:	NP_000289.1	Gene:	PKLR / 5313	HGNCID:	9020	Length:	574	Species:	Homo sapiens

Alignment Length:	483	Identity:	105/483 - (21%)
Similarity:	194/483 - (40%)	Gaps:	84/483 - (17%)

- Green bases have known domain annotations that are detailed below.

  Fly    91 RRMLENGTYTFHVDTVGNKPDELKAI----LDTMNIAISAHSAERELRLTTGLALEINGECCRVG 151
            |....:|::.:|.:::.|..:.:::.    |....:||               ||:..|...|.|
Human   116 RLNFSHGSHEYHAESIANVREAVESFAGSPLSYRPVAI---------------ALDTKGPEIRTG 165

  Fly   152 RLRN--NCTVMLARGGVVTLTTDESYRYKGFKEIVYVINLRCYLASVQLGDIVMIGREVRGKVVK 214
            .|:.  ...|.|.:|..|.:|.|.::|.:|....|:| :....:..|.:|..:.|...:...||:
Human   166 ILQGGPESEVELVKGSQVLVTVDPAFRTRGNANTVWV-DYPNIVRVVPVGGRIYIDDGLISLVVQ 229

  Fly   215 TL-REALTVMIIDAGLVASYDFIELPRQCHALDPDLYPDLY---MKDLEMAASLNANYVVLPKIR 275
            .: .|.|...:.:.|::.|...:.||    ....|| |.|.   ::||........:.|....:|
Human   230 KIGPEGLVTQVENGGVLGSRKGVNLP----GAQVDL-PGLSEQDVRDLRFGVEHGVDIVFASFVR 289

  Fly   276 CKSFLRAVRQTLNSD-FNLKLIGMID-FEYVR--SNMLDL----------LGIIKLVDYIWIPDM 326
            ..|.:.|||..|..: ..:|:|..|: .|.|:  ..:|::          |||....:.:::...
Human   290 KASDVAAVRAALGPEGHGIKIISKIENHEGVKRFDEILEVSDGIMVARGDLGIEIPAEKVFLAQK 354

  Fly   327 FNVNCCVYNYIMEDVLPISQCQKKPVIGTVPLERCSDFKRFELHD----FLWKVDAIHIQKSPWC 387
            ..:..|  |...:.|:..:|..:..:....|       .|.|..|    .|...|.|.:......
Human   355 MMIGRC--NLAGKPVVCATQMLESMITKPRP-------TRAETSDVANAVLDGADCIMLSGETAK 410

  Fly   388 NKYPL-IVKKLMPIKDYRVGAVQNKMVLKSI-----LTSYQTIVNFIIRTISSIEC--QAIFLFT 444
            ..:|: .||....|......||.::.:.:.:     |:...|.|..|....::.:|  .||.:.|
Human   411 GNFPVEAVKMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEAAFKCCAAAIIVLT 475

  Fly   445 TCETASVALSRIEIYCPVYVMVPLEETDDAETITCKVELARALHLRRNMHPVLYTKDLNECSYSP 509
            |...::..|||   |.|...::         .:|...:.||.:||.|.:.|:|| ::..|..::.
Human   476 TTGRSAQLLSR---YRPRAAVI---------AVTRSAQAARQVHLCRGVFPLLY-REPPEAIWAD 527

  Fly   510 -----IEFGVDFMRKKGCLEVGDFVVTL 532
                 ::||::..:.:|.|.|||.|:.:
Human   528 DVDRRVQFGIESGKLRGFLRVGDLVIVV 555

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
CG12229	NP_648980.1	pyruv_kin	103..530	CDD:273424	101/467 (22%)
CG12229	NP_648980.1	Pyruvate_Kinase	126..530	CDD:304951	97/440 (22%)
PKLR	NP_000289.1	Pyruvate_Kinase	85..573	CDD:238178	105/483 (22%)
		Fructose-1,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGF	475..480		2/4 (50%)
		Fructose-1,6-bisphosphate binding, allosteric activator. /evidence=ECO:0000269\|PubMed:11960989, ECO:0007744\|PDB:2VGF	559..564
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0469
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR11817
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	1	0.960	-	-
User_Submission	0	0.000	Not matched by this tool.
	4	3.870