DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Exn and Arhgef5

DIOPT Version :9

Sequence 1:	NP_001097630.2	Gene:	Exn / 39900	FlyBaseID:	FBgn0261547	Length:	2029	Species:	Drosophila melanogaster
Sequence 2:	NP_598435.1	Gene:	Arhgef5 / 54324	MGIID:	1858952	Length:	1581	Species:	Mus musculus

Alignment Length:	1792	Identity:	420/1792 - (23%)
Similarity:	664/1792 - (37%)	Gaps:	469/1792 - (26%)

- Green bases have known domain annotations that are detailed below.

  Fly   482 SVGIPELAIVSEELKT-----------PTSEEQAKKPKSVQNESQPNHPEELNQS------NDKP 529
            |..:|::    |||||           |..:.:|::.:....:....|...:|||      .|..
Mouse    11 STEVPDI----EELKTIPEGIMRSSQIPALDPEAQEDRDPSYKWTDGHRPVMNQSKVLRDMGDHT 71

  Fly   530 SDELDLIMGEEGDEEELRQK-----------QRKHKYASIYEKLRFTFAKGRKSASPSPTKAQIR 583
            .:.:.:...:|..:.|..|:           |::....|:.::|      .|..|:|......::
Mouse    72 PNSMAIFFKKESSDMETSQEILLAEACNTPDQQEAVIQSLKDRL------SRTIAAPELLACAVQ 130

  Fly   584 EE-----VSIDEESIKDSTSEDM-----VTRKEDFTLDLPKEVIPR------------AEDLEDS 626
            ||     ..:|.....:..||.|     |:::::.....|...|||            .|..:.|
Mouse   131 EEWLDIPSKLDNRVGAELQSELMSLTLAVSKEKEEEETSPDTSIPRGSWPPCKTHPGETEQTQGS 195

  Fly   627 SHALL---VSPCFEA--------------DAKILDALEAVDLKLKVLNPLNDVTTADGERELLLA 674
            ...||   .....||              :|:.|:..|..:::::....||:.....|    ||.
Mouse   196 GSELLRQGKQLQLEATQENQGQEGFLQSQEAQGLEEQEGQEVEIQEEGTLNEGICFGG----LLG 256

  Fly   675 ANDDFEQRILPNSSFVYQAANKQYSNNQILLNQAVNVTLVNAIEG-----EQMIMEQKQLMKVRD 734
            ..::.|:....|.....|...:.|   .:|..|..|..|...:||     ......::::..:||
Mouse   257 EQEEVEEGFNGNEEEQKQGQIQSY---MLLGGQWENEGLSGELEGLNYSERGQENRERRVWVLRD 318

  Fly   735 LELTERDIDQPLKRAEKSPEKDDTLEPESLYEPITPVMDETKTEAQTSSLFKIYTKKSELVEDIY 799
            .|  |...||..:..|:........|.:.|.|....|..:.:...||..:..:..:| |:|:
Mouse   319 SE--EEGQDQESREVEERRVATQYTENQRLVEKSEIVKRKQRDHDQTGKVMPVRDQK-EVVD--- 377

  Fly   800 QTVEEASTVEVKPNNWLEGYESIAGSQEACPLTYDGYESFAPEEVVTTPTTPSATGTLGRNTRDE 864
                  |...|:.|....|..::.||:       .|.:|.....|.:......:.|||.......
Mouse   378 ------SGDRVQGNGDSGGQTAVEGSR-------PGEDSKPSLPVASVDPEVLSPGTLFPGISSS 429

  Fly   865 LPELPKPKRVLNKSPI-----PLRPA------------PPKPQAKESEEDENIYDT-------IK 905
            :.::|:    :.|.|:     |..||            ||...|.|...|...:::       .|
Mouse   430 VADIPQ----IQKEPVCEELSPQAPALEPTEWSHQPISPPASFAPEESLDNRTHNSQQEEFRLRK 490

  Fly   906 GCYESMHCKATSSSSSGEATLTTDTISLS-SNCYESISHIRRSRVATTQNQGHGSAGSCIQLSSS 969
            |      .:..|:|:|...:.|.|:...| .|.:.|.:.:             ....|.:.|...
Mouse   491 G------IEVVSASTSVAPSGTRDSPPFSPPNVFSSTATL-------------SPVSSSVILPEE 536

  Fly   970 GSTLTISSD--HKTNSLYESSLAAAGCVVYGSASVGCRSSLGSSAGSRDSGKPGDKRSSIAGSSD 1032
            ..|.:.|:|  |.........|.|.      |:...|.:|        |:..|....||..|.:.
Mouse   537 TPTASASADTPHHCGPCETPPLPAK------SSRYPCATS--------DTANPHSPLSSYTGVTQ 587

  Fly  1033 NSDAWVDISDGEIGQATTDATANEAQFIVVRERFKP--HRT-RSPD---WSKRIRDKRLQQKKAI 1091
            :                            :|....|  ||| ::||   .|.......|.|:...
Mouse   588 H----------------------------LRSNSFPGSHRTEQTPDSLGMSLSFSHLELPQRPPK 624

  Fly  1092 SCIEDDSDHYYETLSPLGNKR---------HSTQLVRQSQDGNRSGGVRSSHRRSKNHSASAHTL 1147
            ..|       |.:|:|..|:|         .||.|....||....  ..:..|.|..|.:.  |.
Mouse   625 PAI-------YGSLTPRRNRRSRDGIVFSDSSTALFALKQDSEEF--TSNPERPSSPHGSP--TW 678

  Fly  1148 GEHVVISDDYDSFETDSDDHGDGEPDLRQRNYNDSGVDM--RNHRLPKPPPPQNQVYEFVRKFKD 1210
            |     |....:|...|..:....|.:        .:||  |...||.||..::.....|.  :|
Mouse   679 G-----SPQNSAFAIGSPANVSSPPTV--------SMDMTIREALLPIPPEKRHSYSHIVE--RD 728

  Fly  1211 FISSKKSPKGSQQKLYENTPASTLF--YVECSKRTEDVYENTDFGPERIPAPET-KGPVHQEQAP 1272
            .:..:.:   |..|.:.:.|..||.  ....||.:..:..::....:..|.|.| :.|.|.:
Mouse   729 GLLHEVA---STLKRHSHPPPLTLSSGLHRSSKGSFSLVPDSTVARQHRPLPSTPESPNHTQ--- 787

  Fly  1273 VLRAKQKNGKSLRSRLR--KSL-------------VGSSFDTKQLSSLAPTRSTFYVEDPEGSLP 1322
                     .|:.||||  |.|             :.||:.::....|.|..    :.||....|
Mouse   788 ---------TSIPSRLRYNKPLPPTPDMPEFYHPSISSSYISRMYRPLPPVP----IIDPSSEPP 839

  Fly  1323 ALPLEPE----------IHGSG------------------------------------------E 1335
            .||.:..          ||..|                                          |
Mouse   840 PLPPKSRGRSKSIQGGVIHSGGQAKPRPNNQDWTASTLSVGRTSWPPATGRSTESLPLTSRCNNE 904

  Fly  1336 LDSGFSDKNCTPL-----PETPKF-----------------------STVARKAKKEAKANRRRT 1372
            :..|.:..|.|.|     |.||..                       .:..|.|.:|...|.||:
Mouse   905 VSPGLAFSNMTNLLSPSSPTTPWIPDLQRPTTKDESGLTEESEPPVRGSFRRSAPQEEFNNTRRS 969

  Fly  1373 TIGLRPNDPPPPPPPMTGNKSPLDPERELDAEQTTSWYAECGVFKQATNGAVSPRGDEPVTPTPS 1437
            .:|.|.|           ::.||..:    .|:.:||                |...:|...:.|
Mouse   970 ALGSRKN-----------SEKPLHHQ----LEKASSW----------------PHRRDPARTSES 1003

  Fly  1438 GHGGV---------SSWYAESGLYQTSGISVASSSGSSGVSTGNEAGLGDEL------------- 1480
            ....|         ..|..: ||.:.| |...|||.....:.|...|..|.:
Mouse  1004 SSEQVVLGQVPNKQKGWNRQ-GLRRPS-ILPESSSDLRNPAAGRLPGSSDSVVFREKKPKEGMGG 1066

  Fly  1481 -SSEPHSLFSNEPLYQMYSAAKLESITRDLEAHGSSDGYEEIGLQAKAKPEPLVKPRPTALQLVE 1544
             |.....|.|::.|||.||...|   .:::::        :..|.:.|:|..|..||.....||.
Mouse  1067 FSRRCSKLISSQLLYQEYSDVVL---NKEIQS--------QQRLDSLAEPHGLSSPRHRRKALVS 1120

  Fly  1545 PKN-------GPSRTLWSEIPEVIHSCILPTLTSRERSLQEAKFEIITSEASYLKSLNLLRRHFM 1602
            ..:       ..|.:||.|||.|.:|.:|.::|..::.|||||||:|.||||||:|||:...||
Mouse  1121 SDSYLQRLSMASSGSLWQEIPVVRNSTVLLSMTHEDQKLQEAKFELIVSEASYLRSLNIAVDHF- 1184

  Fly  1603 NHNAFLDSSVLSAKDRKALFSYIVPVHECSERLLTELEACWQDNIMLLGLSRCIYEIAERHFHVY 1667
            .|:|.| ...||.:|.:.|||.:..|.:.|...|::||..:::||....:...:...|.....||
Mouse  1185 QHSAQL-RGTLSNQDHQWLFSRLQDVRDVSTTFLSDLEENFENNIFSFQVCDVVLNHAADFHRVY 1248

  Fly  1668 VAFCEHQGRMDRTLRRLKESKESLAFQQHLERLEASPSCCGLNLHSFLMLPMQRITRLPLLIDAV 1732
            :.:..:|...:||.:.|..|..|  |::.||:||:.|.|..|:|.|||:||.||||||.||:..:
Mouse  1249 LPYVTNQTYQERTFQSLMNSNSS--FREVLEKLESDPICQRLSLKSFLILPFQRITRLKLLLQNI 1311

  Fly  1733 FSKESPLNREEYESWKLTLALVQKVVGQCNEAANRWEQAFELERIARQLEFPSHVRALAIAPVGV 1797
            ..:..|.:.||.|:.|...|| :|::..||....|..:..||..:::::||...:..|.
Mouse  1312 LKRTQPGSSEEAEATKAHHAL-EKLIRDCNSNVQRMRRTEELIYLSQKIEFECKIFPLI------ 1369

  Fly  1798 PRAGAKPRFLVKRGELTHLLWRGEDAKLTFG--KRLTKISIYAFLFSDLLLLCKRRGESCFSVFD 1860
                ::.|:|||.||||.|     :..::.|  ::||...::..||:|.|||.:.|..|.|.|||
Mouse  1370 ----SQSRWLVKSGELTAL-----EFSVSPGLRRKLTTRPVHLHLFNDCLLLSRPREGSRFLVFD 1425

  Fly  1861 YCPRSMLTLAAGDSLPQLPTKDLK-DQAGKNLILMTLLENCDRKTVELVLSCPSVSDQQRWLQAM 1924
            :.|.|.:   .|:..      ::| ....|||..:.||.|.....||.:....:.|::.||:.|:
Mouse  1426 HAPFSSI---RGEKC------EMKLHGPHKNLFRLFLLHNAQGTQVEFLFRTETQSEKLRWISAL 1481

  Fly  1925 RPPEAETPGEKLYESWDCPQVVAKHSYESDEPDVLQLELGDVVNVSRKLPDGWYQGERIRDGAVG 1989
            ..|..|.   .|.|.:|.|||....:|:..|.|.|.||..|||.|:::..|||.:|.|:.||..|
Mouse  1482 AMPREEL---DLLECYDSPQVQCLRAYKPRENDELALEKADVVMVTQQSSDGWLEGVRLSDGEQG 1543

  Fly  1990 WFPGSYTEELNSAHVRARNLKQRHRLLTFTATYLESQ 2026
            |||....|.:::..|||:|||:.||:.|.....:|.|
Mouse  1544 WFPVQQVEFISNPEVRAQNLKEAHRVKTAKLQLVEQQ 1580

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Exn	NP_001097630.2	RhoGEF	1581..1763	CDD:279015	70/181 (39%)
		PH_ephexin	1805..1931	CDD:269929	41/128 (32%)
		SH3_ephexin1_like	1944..1998	CDD:212727	23/53 (43%)
Arhgef5	NP_598435.1	ARHGEF5_35	1..477	CDD:373847	97/505 (19%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	25..54		2/28 (7%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	159..274		21/118 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	316..643		81/417 (19%)
		PHA03247	<398..949	CDD:223021	121/667 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	659..700		9/49 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	741..810		17/80 (21%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	829..1051		44/258 (17%)
		RhoGEF	1164..1340	CDD:366202	69/180 (38%)
		PH_ephexin	1362..1488	CDD:269929	42/149 (28%)
		SH3_ARHGEF5_19	1498..1552	CDD:212873	23/53 (43%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	118	1.000	Domainoid score	I5824
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	1	1.000	-	-		otm43520
orthoMCL	0	0.000	Not matched by this tool.
Panther	1	1.100	-	-	O	PTHR12845
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	5	4.910