DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment Exn and Ngef

DIOPT Version :9

Sequence 1:	NP_001097630.2	Gene:	Exn / 39900	FlyBaseID:	FBgn0261547	Length:	2029	Species:	Drosophila melanogaster
Sequence 2:	NP_001104784.1	Gene:	Ngef / 53972	MGIID:	1858414	Length:	710	Species:	Mus musculus

Alignment Length:	722	Identity:	218/722 - (30%)
Similarity:	342/722 - (47%)	Gaps:	111/722 - (15%)

- Green bases have known domain annotations that are detailed below.

  Fly  1360 KAKKEAKANRRRTTIG---LRPNDPPP-PPPPMTGNKSPLDPEREL------------------- 1401
            |.:::::::.|::..|   :||..||. ...|.||.::..:..|.|
Mouse    11 KPQRKSESSSRKSNHGPAEMRPALPPENREAPETGEETQNEEPRRLIPIQRHSLFNRAVRHRHKA 75

  Fly  1402 --DAEQTTSWYAECGVFKQATNGAVS---PRGDEPVTPTPSGHGGVSSWYAESGLYQTSGISVAS 1461
              .:|:..|..|:.....::.|...:   |:|..|...||:        ..::|..:.   |.:|
Mouse    76 RSTSERRASDQADLPKMGKSVNERSAFNLPQGRLPPWRTPA--------QRDTGAQEA---SESS 129

  Fly  1462 SSGSSGVSTGNEAGLGDELSSEPHSLFSNEP---------------LYQMY-SAAKLESI-TR-- 1507
            |:..:|.:......|.|..::...:|....|               |||.| ..:.|:.| ||
Mouse   130 STPGNGTTPEECPALTDSPTTLTEALQMIHPIPADSWRNLIEQIGLLYQEYRDKSTLQEIETRRQ 194

  Fly  1508 -DLEAHGSSDGYEEIGLQA--------KAKPEPLVKP--RPTALQLVEPKNGPSR-TLWSEIPEV 1560
             |.|..|:||| .::|..|        :.:.|.|..|  |....|:....|..|| .||.::||:
Mouse   195 QDAEIQGNSDG-SQVGEDAGEEEEEEEEGEEEELASPPERRALPQICLLSNPHSRFNLWQDLPEI 258

  Fly  1561 IHSCILPTLTSRERSLQEAKFEIITSEASYLKSLNLLRRHFMNHNAFLDSSVLSAKDRKALFSYI 1625
            ..|.:|..|...|..||||.||::||||||.||||||..|||.:...  ..:|...:...|||.:
Mouse   259 QSSGVLDILQPEEIRLQEAMFELVTSEASYYKSLNLLVSHFMENERL--KKILHPSEAHILFSNV 321

  Fly  1626 VPVHECSERLLTELEACWQDNIMLLGLSRCIYEIAERHFHVYVAFCEHQGRMDRTLRRLKESKES 1690
            :.|...|||.|.|||...::||::..:...:|..|..||.||:.:..:|...:||.::|.:  |.
Mouse   322 LDVMAVSERFLLELEHRMEENIVISDVCDIVYRYAADHFSVYITYVSNQTYQERTYKQLLQ--EK 384

  Fly  1691 LAFQQHLERLEASPSCCGLNLHSFLMLPMQRITRLPLLIDAVFSKESPLNREEYESWKLTLAL-- 1753
            .||::.:.:||..|.|.||...|||:||.||||||.||:..:      |.|.|..|.:...||
Mouse   385 AAFRELIAQLELDPKCKGLPFSSFLILPFQRITRLKLLVQNI------LKRVEERSEREGTALDA 443

  Fly  1754 ---VQKVVGQCNEAANRWEQAFELERIARQLEFPSHVRALAIAPVGVPRAGAKPRFLVKRGELTH 1815
               ::.||..|||...:..:..::..|.:::||  .::::.|.        :..|:|:|:|||..
Mouse   444 HKELEMVVKACNEGVRKMSRTEQMISIQKKMEF--KIKSVPII--------SHSRWLLKQGELQQ 498

  Fly  1816 LLWRGEDAKLTFGKRLTKISIYAFLFSDLLLLCKRRGESCFSVFDYCPRSMLTLAAGDSLPQLPT 1880
            :  .|.....|...:.....||.|||:|||::|::.....:.|||..||.:|.:
Mouse   499 M--SGPKTSRTLRTKKLFREIYLFLFNDLLVICRQIPGDKYQVFDSAPRGLLRV----------- 550

  Fly  1881 KDLKDQAG--KNLILMTLLENCDRKTVELVLSCPSVSDQQRWLQAMRPPEAETPGEKLYESWDCP 1943
            ::|:||..  .|:.::.||||.|.:....:|...|.|:.:||:.::.|..............|||
Mouse   551 EELEDQGQTLANVFILRLLENADDREATYMLKASSQSEMKRWMTSLAPNRRTKFVSFTSRLLDCP 615

  Fly  1944 QVVAKHSYESDEPDVLQLELGDVVNVSRKLPDGWYQGERIRDGAVGWFPGSYTEELNSAHVRARN 2008
            ||...|.|.:.:||.|.|||.|::|:..|..|||..|||:.|...||||.|.|||:.:..:|::|
Mouse   616 QVQCVHPYVAQQPDELTLELADILNILEKTEDGWIFGERLHDQERGWFPSSMTEEILNPKIRSQN 680

  Fly  2009 LKQRHRL 2015
            ||:..|:
Mouse   681 LKECFRV 687

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
Exn	NP_001097630.2	RhoGEF	1581..1763	CDD:279015	72/186 (39%)
		PH_ephexin	1805..1931	CDD:269929	38/127 (30%)
		SH3_ephexin1_like	1944..1998	CDD:212727	26/53 (49%)
Ngef	NP_001104784.1	Regulatory region, modulates activity toward RHOA, RAC1 and CDC42	1..272		61/272 (22%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	1..146		27/145 (19%)
		ELH	3..>156	CDD:145463	28/155 (18%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	192..234		12/42 (29%)
		RhoGEF	277..456	CDD:279015	73/188 (39%)
		PH_ephexin	477..603	CDD:269929	39/146 (27%)
		PH	490..596	CDD:278594	36/118 (31%)
		SH3_ephexin1	616..670	CDD:212872	26/53 (49%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	688..710		218/722 (30%)

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	1	1.000	118	1.000	Domainoid score	I5824
eggNOG	1	0.900	-	-		E1_COG5422
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	1	1.000	-	-		FOG0001805
OrthoInspector	1	1.000	-	-		otm43520
orthoMCL	1	0.900	-	-		OOG6_105893
Panther	1	1.100	-	-	O	PTHR12845
Phylome	1	0.910	-	-
RoundUp	1	1.030	-	avgDist	Average_Evolutionary_Distance	R3601
SonicParanoid	1	1.000	-	-		X1705
SwiftOrtho	0	0.000	Not matched by this tool.
TreeFam	0	0.000	Not matched by this tool.
	9	8.840