Protein Alignment Exn and RhoGEF2

DIOPT Version :9

• Sequence 1: NP_001097630.2
• Gene: Exn / 39900
• FlyBaseID: FBgn0261547
• Length: 2029
• Species: Drosophila melanogaster
• Sequence 2: NP_477317.1
• Gene: RhoGEF2 / 36915
• FlyBaseID: FBgn0023172
• Length: 2559
• Species: Drosophila melanogaster
• Alignment Length: 2155
• Identity: 413/2155 - (19%)
• Similarity: 734/2155 - (34%)
• Gaps: 703/2155 - (32%)

  Fly   120 SASEDQCHSPVHCHTSSSSLGTLKKLNKSVSCLVNAFNNSRDCATPEKTLAKR---------AAS 175
            ||...|  |||....|...|...|:  |||       .||:. ...:|.||.:         |:|
  Fly    66 SAGAPQ--SPVIVVDSVPELPAPKQ--KSV-------KNSKS-KQKQKQLANKSKIPRSPSLASS 118

  Fly   176 LHNLQDSQAKHKQLHREKDNFYKYKNA------------EQAKMQAKLRSSADEAI-LNASETRA 227
            |.:|..|.:.|:...:::|...:.:||            :|.:|.....::|...: ..|:.|.|
  Fly   119 LSSLASSLSGHRDRDKDRDKDRENQNAVPPQTPPLPPSYKQNQMNGDSTAAAGGGVSAPATPTTA 183

  Fly   228 KRDDSGCLPGVKSYNSKALSAADKVAKVAKGEAKTQRRAEEPVGEAEVEVEVEFRAKREHNRLEG 292
            ..:::       |:|:.::        :..|....|.....||.:|..|             ...
  Fly   184 NNNNA-------SHNNGSI--------MGGGVQLNQSDNSNPVLQAPGE-------------RSS 220

  Fly   293 LSLSAASAQ-SGGSSESLQTTTTSSSTPTTHSSVTSSTGRSGGRRKYSFKTHAGKSYHQHHHPSR 356
            |:|:..|.. |||.:   |.:||.::||:|.|....                  |::.......|
  Fly   221 LNLTPLSRDLSGGHT---QESTTPATTPSTPSLALP------------------KNFQYLTLTVR 264

  Fly   357 RTSN-----MDSSN---------GGISMVAKLTHQFNEIIQKDAKLLEQVKRNNGVWISRGTHVY 407
            :.||     :...|         ||.:.:|.|...            :.:.|.||       |..
  Fly   265 KDSNGYGMKVSGDNPVFVESVKPGGAAEIAGLVAG------------DMILRVNG-------HEV 310

  Fly   408 KIVEKPTSEGKGLPNADDNRISTVQRNIKKFERLEKPSVPLKSEQLMRKHLELMRG----SSLSR 468
            ::.:.||.  .||..|.    :||:..:|:.::|.:||    |..::.....::.|    :|::
  Fly   311 RLEKHPTV--VGLIKAS----TTVELAVKRSQKLTRPS----SVSVVTPSTPILSGRDRTASIT- 364

  Fly   469 GPRIKRNLKLPPGSVGIPELAIVSEELKTPTSEEQAKKPK-SVQN-ESQPNHP------------ 519
            ||:       |..|:...|:    |..|..|.::..::.| :::. :|..|:|
  Fly   365 GPQ-------PVDSIKRREM----ETYKIQTLQKMLEQEKLNLERLKSDQNNPSYKLSEANIRKL 418

  Fly   520 -EELNQ--SNDKPSDELDLIMGEEGDEEELRQKQRKHKYAS-IYEKLRF------------TFAK 568
             |:|:|  :.|.|:.:|....|.: :...|...|.:|..|| .:...:|            .:..
  Fly   419 REQLHQVGAEDAPTVKLQAAAGNK-NTALLTPNQIQHLSASATHSNQQFHHLHHHHNLHNNNYPP 482

  Fly   569 GRKSASPSPTKAQI--REEVSIDEESIKDSTSEDMVTRKE-DFTLDL--PKEVIPRAEDLEDSSH 628
            .::.||.||....:  |...|:...:.|:.|.:|:.|... ..|.|.  .:.:..:||.:..|.|
  Fly   483 QQQPASTSPAFLSLLPRSLSSLSLGTRKNKTEKDLTTSSPFGLTTDFLQQQRMSHQAESMSQSMH 547

  Fly   629 ALLVSPC--------------------------FEADAKILDALEAVDLKLKVLNP-------LN 660
            ....:|.                          |.....:::  |.|...|...||       |.
  Fly   548 QHTSTPTSQQFFHPHQQQHRFKETGPTSKGKNKFLISRSLIE--EDVPPPLPQRNPPRQLNLDLK 610

  Fly   661 DVTTADGERELLLAAND----------DFEQRILPNSSFVYQAANKQYSNNQILLNQAVNVTLVN 715
            :...:.|...|:...:|          ..:|:.||:|:      :...||.:...::.....|.:
  Fly   611 NGNASPGGSHLVAPVSDLDRATSPQLNRSQQQQLPSST------DNSPSNAKSKRSKIKTKALSD 669

  Fly   716 AIEGEQMIMEQKQLMKVRDLELTERDIDQPLKRAEKSPEKDDTLEPESLYEPITPVMDETKTE-- 778
            .....||.::                    ::.|..:.....::|.:....|:.|.:....||
  Fly   670 PKMSTQMFLQ--------------------MESASAAGAAGGSIEVDGGPPPLPPRLPGMMTEDM 714

  Fly   779 --------AQTSSL-----FKIYTKKSELVEDIYQTVEEASTVEVKPN--NWLEGYE-----SIA 823
                    ||.:|:     :.:.:..:.:..|   .:..|..:..:||  ..|:.|:     .::
  Fly   715 SRGSCQNLAQPNSVGTAFNYPLVSTTTAVQND---NLNIAFPLSQRPNIVQQLQQYQQQQQHQMS 776

  Fly   824 GSQEACPLTYDGYESFAPEEVVTTPTTPSATGTLGRNTRDELPELPKPK-RVLNKSPIPLRPAPP 887
            |.|                          |||.||     :.|.|.|.| |.:..||..:.|..|
  Fly   777 GGQ--------------------------ATGALG-----QTPNLGKNKHRRVGSSPDNMHPRHP 810

  Fly   888 KPQAKESEEDENIYDTIKGCYESMHCKATSSSSSGEATLTTDTISLSSNCYESISHIRRSRVATT 952
                      :.|..|..|.:|.:. |...||..|    |.....|||      ||:........
  Fly   811 ----------DRITKTTSGSWEIVE-KDGESSPPG----TPPPPYLSS------SHMTVLEDPNE 854

  Fly   953 QNQGHGSAGSCIQLSSSGSTLTISSDHKTNSLYESSLAAAGCVVYGSASVGCRSSLGSSAGSRDS 1017
            .|:|..:||..:.:.|...|               .:|.|...:    .:...||...:|.|.|:
  Fly   855 NNRGAAAAGPGVFIESHQFT---------------PMAGASSPI----PISLHSSHMHAAQSNDT 900

  Fly  1018 GKPGDKRSSIAGSSDNSD---AWVDISDGEIGQATTDATANEAQFI------------------- 1060
                 ::..|:...:|||   .::| .:|.....|....|....|:
  Fly   901 -----QKEIISMEDENSDLDEPFID-ENGPFNNLTRLLEAENVTFLAIFLNYVISNSDPAPLLFY 959

  Fly  1061 VVRERFKP-------------HRT----RSP-DWSKRIRDKRL-QQKKAISCIEDDSDHYYETLS 1106
            ::.|.:|.             |.|    |:| .|.:  :|:.| ::...:..:|.|......|:.
  Fly   960 LITELYKEGTSKDMRKWAYEIHSTFLVPRAPLSWYR--QDESLAREVDNVLQLEYDKVEILRTVF 1022

  Fly  1107 PLGNKRHS---TQLVRQSQDGNRSGGVRSSHRRSKNHSASAHT--LGEHVVISDDY---DSFETD 1163
            ....||..   ::.:|:.|. .|:.|:.:.:..:.:..|.|.|  |.|.::  |.|   :.....
  Fly  1023 LRSRKRAKDLISEQLREFQQ-KRTAGLGTIYGPTDDKLAEAKTDKLREQII--DKYLMPNLHALI 1084

  Fly  1164 SDDHGDGEPDLRQ------------RNYNDSGVDMRNHRLPKPPPPQ--NQVYEFVRKFKDFIS- 1213
            .|::|....|:|:            |.:|           .:|||..  .:|:.||.:.|.|.|
  Fly  1085 EDENGSPPEDVRKVALCSALSTVIYRIFN-----------TRPPPSSIVERVHHFVSRDKSFKSR 1138

  Fly  1214 ----SKK-----------------------------SPKGSQQKLYENTPASTLFYVECSKRTED 1245
                ::|                             ||:|     |..|......:.:|||..::
  Fly  1139 IMGKNRKMNVRGHPLVLRQYYEVTHCNHCQTIIWGVSPQG-----YHCTDCKLNIHRQCSKVVDE 1198

  Fly  1246 VYENTDFGPERIPAPETKGPVHQEQAPVLRAKQKN------GKSLRSRLRKSL-------VGSSF 1297
            ....        |.|:.|...|.::......|.:.      |...|||..:.|       .|.:.
  Fly  1199 SCPG--------PLPQAKRLAHNDKISKFMGKIRPRTSDVIGNEKRSRQDEELDVELTPDRGQAS 1255

  Fly  1298 DTKQLSSLAP-----TRSTFYVEDPEGSLPALPLEPEIHGS-------------GELDSGFSDKN 1344
            ..:|.|...|     .||..........|....|:...|||             ..:|...|..:
  Fly  1256 IVRQPSDRRPDANISIRSNGNTSCNTSGLNTTDLQSSFHGSCANDSINPGGGAGCNMDLSTSVAS 1320

  Fly  1345 CTP------------LPETPKFSTVARKAKKEAKANRRRTTIGLRPNDPPPPPPPMTGNKSP--- 1394
            .||            ..|.....||.::|::|..:..           |.....|::.|:|.
  Fly  1321 TTPSTSGSVAAGLSAFAELNALDTVDKEARRERYSQH-----------PKHKSAPVSVNRSESYK 1374

  Fly  1395 --LDPERELDAEQTTSWYAECGVFKQATNGAVSPRGDEPVTPTPSGH----GGVSSWYAESGLYQ 1453
              |..:|..::.:.||                    |..::..|:..    |..::.|..|
  Fly  1375 ERLSNKRNRNSRRKTS--------------------DPSLSSRPNDEQLDLGLSNATYVGS---- 1415

  Fly  1454 TSGISVASSSGSSGVSTGNE-------AG------LGDELSSEPHSLFSNEPLYQMYSAAKLESI 1505
             |..|::|:.||...||..|       ||      :|...:..||.|.....  |.|  .:.:|.
  Fly  1416 -SNSSLSSAGGSESPSTSMEHFAAPGAAGGVQVPPMGLNQNQHPHLLIQQHA--QQY--CQQDSF 1475

  Fly  1506 TRDLEAHGSSDGYEEIGLQAKAKPEPLVKPRPTALQLVEPKNGPSRTLWSEIPEVIHSCILPTLT 1570
            ...|.....|.............|.|:.:   ..|: .|.::..:...||   .::.:.:|..||
  Fly  1476 QAGLAGAAGSSAASNSSFWNAGHPLPVAR---WTLE-SEDEDDVNEADWS---SMVAAEVLAALT 1533

  Fly  1571 SRERSLQEAKFEIITSEASYLKSLNLLRRHFMNHNAFLDSSVLSAKDRKALFSYIVPVHECSERL 1635
            ..|:..||...||..:|.:::::|.||.|.|                       .:|::|..  |
  Fly  1534 DAEKKRQEIINEIYQTERNHVRTLKLLDRLF-----------------------FLPLYESG--L 1573

  Fly  1636 LTELEACWQDNIMLL-------------GLSRCIYEIAERHFHVY----------------VAFC 1671
            |:      ||:::||             ...:.:.:....|.||.                |..|
  Fly  1574 LS------QDHLLLLFPPALLSLREIHGAFEQSLKQRRIEHNHVVNTIGDLLADMFDGQSGVVLC 1632

  Fly  1672 EHQ----GRMDRTLRRLKE--SKESLAFQQHLERLEASPSCCGLNLHSFLMLPMQRITRLPLLID 1730
            |..    .|....|..|||  :|:.: .|:.|::.|:..:|..|.|...|...:||:|:.|||.:
  Fly  1633 EFAAQFCARQQIALEALKEKRNKDEM-LQKLLKKSESHKACRRLELKDLLPTVLQRLTKYPLLFE 1696

  Fly  1731 AVFS---KESPLNREEYESWKLTLALVQKVVGQCNEAANRWEQAFELERIARQLEFPSHVRALAI 1792
            .::.   :..|.|..|.|:.:..:...::::.:.|:|....|.|.:|:.|.|:|:..|:
  Fly  1697 NLYKVTVRLLPENTTEAEAIQRAVESSKRILVEVNQAVKTAEDAHKLQNIQRKLDRSSY------ 1755

  Fly  1793 APVGVPRAGAKPRFLVKRGELT--HLLWRGEDAKLTFGKRLTKISIYAFLFSDLLLLCKRRGESC 1855
                     .|..|  |:.:||  ||:   .|..||. |:...:.::..||.::::|..::.:.
  Fly  1756 ---------DKEEF--KKLDLTQHHLI---HDGNLTI-KKNPSVQLHGLLFENMIVLLTKQDDK- 1804

  Fly  1856 FSVFDYCPRSMLTLAAGDSLPQLPTKDL-------KDQAGKNLILMTLLENCDRKTVELV-LSCP 1912
                 |..:::.|..:..:.|..|...:       ::.|.||...:..:     ||.::: |..|
  Fly  1805 -----YYLKNLHTPLSITNKPVSPIMSIDADTLIRQEAADKNSFFLIKM-----KTSQMLELRAP 1859

  Fly  1913 SVSDQQRWLQ 1922
            |.|:.:.|.:
  Fly  1860 SSSECKTWFK 1869

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
Exn	NP_001097630.2	RhoGEF	1581..1763	CDD:279015	45/219 (21%)
		PH_ephexin	1805..1931	CDD:269929	27/128 (21%)
		SH3_ephexin1_like	1944..1998	CDD:212727
RhoGEF2	NP_477317.1	PDZ	260..334	CDD:214570	19/98 (19%)
		RGS_GEF_like	934..1055	CDD:188710	20/123 (16%)
		C1_1	1151..1202	CDD:278556	8/55 (15%)
		RhoGEF	1539..1731	CDD:238091	47/223 (21%)
		PH_RhoGEF	1770..1875	CDD:275411	22/115 (19%)
		DUF2413	2242..>2378	CDD:287302

Information from Original Tools

Tool	Simple Score	Weighted Score	Original Tool Information
			BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG5422
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
	2	1.810