Protein Alignment LIG4 and DNAlig1

DIOPT Version :10

• Sequence 1: NP_001339533.1
• Gene: LIG4 / 3981
• HGNCID: 6601
• Length: 923
• Species: Homo sapiens
• Sequence 2: NP_611843.2
• Gene: DNAlig1 / 37791
• FlyBaseID: FBgn0262619
• Length: 747
• Species: Drosophila melanogaster
• Alignment Length: 682
• Identity: 157/682 - (23%)
• Similarity: 283/682 - (41%)
• Gaps: 133/682 - (19%)

Human    31 LCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHKNHKDVTDSFYPAMRLILPQLERERMAYG 95
            |..|.:.|:::|||.:.|.....|      |...:..:.:|:..|.|.::..:.|..  |.:..|
  Fly   130 LARTFQVIEETKGRLKMIDTLSNF------FCSVMLVSPEDLVPSVYLSINQLAPAY--EGLELG 186

Human    96 IKETMLAKLYIELLNLPRDGKDALKLLNYRTPTGTHGDAGDFAMIAYFVLKPRCLQKGSLTIQQV 160
            :.||.|.|...:     ..|::   |.:.::.|...||.|..|..:. |.:....|...|.::.|
  Fly   187 VAETTLMKAICK-----ATGRN---LAHIKSQTQLTGDLGIVAEQSR-VSQRMMFQPAPLNVRDV 242

Human   161 NDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVSQQTIFSVFHNDAAE 225
            ...|..||..:...:.||:    ..:.....:.|.::.||.:|..|::|:::|::.:.......:
  Fly   243 FRKLREIAKLSGQSKMDLV----YNMFVACRSSEARFFIRSLIGKLRIGIAEQSLLTALAIGLVK 303

Human   226 LHNVTTDLEKVCRQLHDPSV---GLSDISITLFSAF----------------------------- 258
            .:::..     |:....|.|   .:.|.::.|.:|:
  Fly   304 KNHIDD-----CKASKVPDVYKDEIVDTTLLLKTAYCQCPNYDIIIPAILKYDIKELQERCPMHP 363

Human   259 ----KPMLA-AIADIEHIEKDMKHQSFYIETKLDGERMQMHKD--GDVYKYFSRNGYN----YTD 312
                :|||| ....:..:.:.........|.|.||||.|:|::  |:: ..||||..|    |.|
  Fly   364 GMPLRPMLAQPTKGVHEVFERFGGMQITCEWKYDGERAQIHRNEKGEI-SIFSRNSENNTAKYPD 427

Human   313 QFGASPTEGSLTPFIHNAFKADIQICILDGEMMAYNPNTQTFM-------QKGTKFDIKRMVEDS 370
            ....|..          ..|.|::..|:|.|::|::...:..:       :|....||    |:.
  Fly   428 LIARSTA----------LLKGDVKSYIIDSEIVAWDVERKQILPFQVLSTRKRKNVDI----EEI 478

Human   371 DLQTCYCVFDVLMVNNKKLGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAID 435
            .:|.|..:||:|.:|...|..:.|.:|.::|...|..:.|..:.......:..:||...|.|:|.
  Fly   479 KVQVCVYIFDLLYINGTALVTKNLSERRKLLLEHFQEVEGEWKFATALDTNDIDEVQQFLEESIK 543

Human   436 KREEGIMVK--QPLSIYKPDKRGEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCA 498
            ...||:|||  ...:.|:..||...|||:|.:|:|.:.|.||::::|||.|||.|.|....||.|
  Fly   544 GNCEGLMVKTLDEEATYEIAKRSRNWLKLKKDYLSNVGDSLDLVVIGGYKGKGRRTGTYGGFLLA 608

Human   499 VAEKPPPGEKPSVFHTLSRVGSGCTMKELYD----LGLKLAKYWKPFHRKAPPSSILCGTEKPEV 559
            ..:     .:...:.::.::|:|.|.::|..    ||..:....|.::|..|       :.:|:.
  Fly   609 CYD-----TENEEYQSICKIGTGFTDEDLQTHSEFLGKHVTSAAKSYYRYDP-------SLEPDH 661

Human   560 YIEPCNSVIVQIKAAEIVPSDMYKT---------GCTLRFPRIEKIRDDKEWHECMTLDDLEQLR 615
            :.||..  :.::|.|::..|.:::.         |.:|||||..:|||||.....   .|..|:
  Fly   662 WFEPVQ--VWEVKCADLSLSPIHRAAIGIVDGERGISLRFPRFIRIRDDKNSENA---TDANQV- 720

Human   616 GKASGKLASKHLYIGGDDEPQEKKRKAAPKMK 647
                     .|:|...|.....:|.....:|:
  Fly   721 ---------AHMYQSQDQVKNNQKSSTQMEME 743

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
LIG4	NP_001339533.1	dnl1	93..614	CDD:273147	137/585 (23%)
		BRCT_DNA_ligase_IV_rpt1	670..757	CDD:349354
		DNA_ligase_IV	762..795	CDD:463272
		BRCT_DNA_ligase_IV_rpt2	825..912	CDD:349349
DNAlig1	NP_611843.2	PLN03113	1..745	CDD:215584	157/682 (23%)