Protein Alignment LIG4 and DNAlig4

DIOPT Version :10

• Sequence 1: NP_001339533.1
• Gene: LIG4 / 3981
• HGNCID: 6601
• Length: 923
• Species: Homo sapiens
• Sequence 2: NP_572907.1
• Gene: DNAlig4 / 32322
• FlyBaseID: FBgn0030506
• Length: 918
• Species: Drosophila melanogaster
• Alignment Length: 951
• Identity: 286/951 - (30%)
• Similarity: 471/951 - (49%)
• Gaps: 135/951 - (14%)

Human    30 DLCSTLERIQKSKGRAEKIRHFREFLDSWRKFHDALHK-------NHKDVTDSFYPAMRLILPQL 87
            |:||..|:|:.::..|.|....:.:.:|:.:..::..:       ..:|...|||..:||:||..
  Fly    13 DICSLFEKIKATQKVANKEEVLKSYYESFCRHRESFRRQTGLNNDQPEDGASSFYSVLRLLLPGA 77

Human    88 ERERMAYGIKETMLAKLYIELLNLPRDGKDALKLLN-----YRTPTGTHGDAGDFAMIAYFVLKP 147
            :..|..||::.|.|.:|||.:|.||.|..||::|.:     ||       |.||   :.|.||||
  Fly    78 DTGRDTYGLQITALGRLYIRVLQLPTDSSDAIRLQHRNGNMYR-------DYGD---VVYSVLKP 132

Human   148 RCLQKGS-LTIQQVNDLLDSIASNNSAKRKDLIKKSLLQLITQSSALEQKWLIRMIIKDLKLGVS 211
            ||....| |.:::::.:||:||:.::    ::.::.|::...|:|..|||||||:::|.|.||:.
  Fly   133 RCFNPPSNLRLKEIHQMLDTIANEDT----EVKQQQLIRFTEQASPEEQKWLIRLLLKSLGLGIG 193

Human   212 QQTIFSVFHNDAAELHNVTTDLEKVCRQLHDPSVGLSDIS----------------ITLFSAFKP 260
            :|.||.|.|..|.:::...:||..||..|.|.:..|...|                |..|...:|
  Fly   194 EQKIFGVLHPKAQDIYQRCSDLGHVCNLLADRTTDLDASSSKDSKAAVKFVNLNSVIRPFHQIRP 258

Human   261 MLAA--IADIEHIEKDMKHQSFYIETKLDGERMQMHKDGDVYKYFSRNGYNYTDQFGASPTEGSL 323
            ||..  ..||:.:   |:....|:|||:||||.|:|.|...:.|.||||.:||..||.|...|:|
  Fly   259 MLCERFPGDIQEL---MQSDVLYLETKMDGERFQLHIDRGRFMYISRNGVDYTRNFGHSYDHGTL 320

Human   324 TPFIHNAFKADIQICILDGEMMAYNPNTQTFMQKGTKFDIKRMVEDSDLQTCYCVFDVLMVNNKK 388
            ||.:.......::..|||||||.::.|...|.:||...|:|.:..:...|.|:.|:|:|..|.:.
  Fly   321 TPKLRGLLPLGLESIILDGEMMVWDTNKLRFREKGENTDVKSLKPEGSWQPCFVVYDLLYFNGQS 385

Human   389 -LGHETLRKRYEILSSIFTPIPGRIEIVQKTQAHTKNEVIDALNEAIDKREEGIMVKQPLSIYKP 452
             |.|..:::.|: |..:.....|.:::::..:..:..|..:...:|:|...|||::|:..|.|:|
  Fly   386 LLDHTYIQRAYK-LQKLIVEQSGVLQLMRARKIGSVQEFNELFQQALDSHAEGIVLKKQGSRYQP 449

Human   453 DKR-GEGWLKIKPEYVSGLMDELDILIVGGYWGKGSRGGMMSHFLCAVAEKPPPG--EKPSVFHT 514
            ..| |.||.|.|.:|:.||:.|.|:||:|.::.:  :...:..||..|.:..|||  .:|.|| :
  Fly   450 GVRLGGGWYKDKADYIKGLITEFDVLIIGAFYNR--KRTFVDSFLLGVLQPAPPGSSNRPEVF-S 511

Human   515 LSRVGSGCTMKELYDLGLKLAKYW-----KP----FHRKAPPSSILCGTEKPEVYIEPCNSVIVQ 570
            :..|.:....:.:.:..||  .:|     :|    ||.| |.....|    |:::|||.||||:|
  Fly   512 IGVVANNTKQRGVLNHTLK--PHWHDVVNEPPPLWFHYK-PKERSGC----PDLWIEPQNSVILQ 569

Human   571 IKAAEIVPSDMYKTGCTLRFPRIEKIRDDKEWHECMTLDDLEQLRG--KASGKLASKHLYIGGDD 633
            :|||::.|:..:.|..:|.|||.|..||||.|.|||||.:...|.|  .|..||..:.|.: .|.
  Fly   570 VKAADLAPNGAFFTRKSLHFPRTEMKRDDKTWSECMTLKEFNDLCGGPLAIKKLNKRQLRL-EDV 633

Human   634 EPQEKKRKAAPKMKKVIGIIEHLKAPNLTNVNKISNIFEDVEFCVMSGTDSQ-PKPDLENRIAEF 697
            ..:.|:.:..|..:..:|:..:.|..:.:.....|.:|:.:.||::||:..: .|..|:....:.
  Fly   634 TTKRKQMRMTPSERSRLGLAVYEKRYDASTSASTSKLFDGLSFCILSGSAGRHSKHQLQELAVKN 698

Human   698 GGYIVQNP---GPDTYCVIAGSENIRVKNIILSNKH--DVVKPAWLLE-CFKTKSFVPWQPRFMI 756
            ||.||:||   .|..:| |||.|...||.:||....  |:|:..|||. |.|.:  :..:||.:|
  Fly   699 GGCIVENPLPNDPKCFC-IAGDETFLVKRLILQQPRTCDIVRMEWLLRVCQKQE--LELKPRDLI 760

Human   757 HMCPSTKEHFAREYDCYGDSYFID-TDLNQLKEVFSGIKNSNEQTPEEMASLIAD----LEYRYS 816
            ......::..|..:|.:||||..| .::.:|:::..||    |.|.:.:|.:.|.    ||.:..
  Fly   761 AATEPLQQDLAECFDRHGDSYTKDIANVEELQDLLQGI----ELTADNVAGITASNLNALEDQLL 821

Human   817 WDCSPLSMFRRHTVYLDSYAVINDLSTKNEGTRLAIKALELRFHGAKVVS-----------CLAE 870
            .....|:|||.          :|.......|..:| |.|.|: :|.::|.           |::.
  Fly   822 DGKKNLNMFRN----------LNAFFYSPHGDEVA-KLLFLQ-NGGRIVDDSDPQLNLGFICMSS 874

Human   871 GV-----SHVIIGEDHSRVADFKAFRRTFKRKFKILKESWV 906
            .:     .|.:  .:||::.           ..|:|..:|:
  Fly   875 DIDNDHFEHWL--HNHSKLT-----------TDKVLNSAWI 902

Known Domains

Gene	Sequence	Domain	Region	External ID	Identity
LIG4	NP_001339533.1	dnl1	93..614	CDD:273147	189/557 (34%)
		BRCT_DNA_ligase_IV_rpt1	670..757	CDD:349354	32/93 (34%)
		DNA_ligase_IV	762..795	CDD:463272	10/33 (30%)
		BRCT_DNA_ligase_IV_rpt2	825..912	CDD:349349	17/98 (17%)
DNAlig4	NP_572907.1	dnl1	84..613	CDD:273147	189/556 (34%)
		Adenylation_DNA_ligase_IV	249..466	CDD:185713	74/220 (34%)
		BRCT	670..760	CDD:469589	32/92 (35%)
		BRCT_2	830..917	CDD:465186	17/98 (17%)