DRSC - DRSC Integrative Ortholog Prediction Tool

back to: DIOPT - Ortholog Prediction Tool / DIOPT for Diseases and Traits

Protein Alignment DNApol-delta and Pola1

DIOPT Version :9

Sequence 1:	NP_524099.2	Gene:	DNApol-delta / 39746	FlyBaseID:	FBgn0263600	Length:	1092	Species:	Drosophila melanogaster
Sequence 2:	NP_032918.1	Gene:	Pola1 / 18968	MGIID:	99660	Length:	1465	Species:	Mus musculus

Alignment Length:	1222	Identity:	292/1222 - (23%)
Similarity:	493/1222 - (40%)	Gaps:	276/1222 - (22%)

- Green bases have known domain annotations that are detailed below.

  Fly    13 GHAKKPRNPDDDEEMG-FEAELAAFENSEDMDQTLLMGDGPENQTTSERWSRPPPP------ELD 70
            |...:|..|:|::|:| .|.|...|:.|.|.::.      .|...|::.|.:...|      |.|
Mouse   236 GELAQPECPEDEQELGVMEFEDGDFDESMDTEKV------DEKPVTAKTWDQETEPVERVEHEAD 294

  Fly    71 PSKHNLEFQQLDVENYL----------------------GQPLPGMPGAQIGPV----------- 102
            |.:....:    :||:|                      ...||.:.||....|
Mouse   295 PERGTTSY----LENFLPDVSCWDIDQDDESIPQEVQVDSSNLPLVKGADDEQVFQFYWLDAYED 355

  Fly   103 -----PVVRMFG-VTMEGNSVCCHVHGFC--------PYFYIEAPSQFEEHHCEKLQKALDQKVI 153
                 .||.:|| |.:|  ||..|| ..|        ..:::....:|:.:..::....:..|.:
Mouse   356 PYNQPGVVFLFGKVWIE--SVKTHV-SCCVMVKNIERTLYFLPREMKFDLNTGKETAIPVTMKDV 417

  Fly   154 ADIRNNKDNVQEAVLMV--ELVEKLNIHGY-----NGDKKQRYIKISVTLPRFVAAASRL---LK 208
            .:..::|.:.:..::..  ::|||    .|     :..:|..|:::     |:.|...:|   ||
Mouse   418 YEEFDSKISAKYKIMKFKSKIVEK----NYAFEIPDVPEKSEYLEV-----RYSAEVPQLPQNLK 473

  Fly   209 KEVIMSEIDFQDCRAFENNIDFDIRFMVDTDVVGCNWIELPMGHWRIRNSHSKPLPESRCQIEV- 272
            .|..        ...|..|......|:::..:.|..|:|       ::|......|.|.|:.||
Mouse   474 GETF--------SHVFGTNTSSLELFLMNRKIKGPCWLE-------VKNPQLLNQPISWCKFEVM 523

  Fly   273 -----------DVAFDRFISHEPEGEWSKVAPFRILSFDIECAGRKGIFPEAKIDPVIQIANMV- 325
                       ||:               ..|..::||.::....    .:.....:|.:|.:|
Mouse   524 ALKPDLVNVIKDVS---------------PPPLVVMSFSMKTMQN----VQNHQHEIIAMAALVH 569

  Fly   326 ----IRQGEREPFIRNVFTL----NECA-PIIGSQVLCHDK--------ETQMLDKWSAFVREVD 373
                :.:...||..:..|.:    .:|. |....:|:....        |..::..:.|.|.::|
Mouse   570 HSFALDKAPPEPPFQTHFCVVSKPKDCIFPCDFKEVISKKNMKVEIAATERTLIGFFLAKVHKID 634

  Fly   374 PDILTGYNINNFDFPYLLNRAAHLKVRNFEYLGRIKNIRSVIKEQMLQSKQ-MGRRENQYVNFEG 437
            ||||.|:||.:|:...||.|....||..:..:||::  ||.:.:  |.|:. .|.|....    |
Mouse   635 PDILVGHNICSFELEVLLQRINECKVPYWSKIGRLR--RSNMPK--LGSRSGFGERNATC----G 691

  Fly   438 RVPFDLLFVLLRDYKLRSYTLNAVSYHFLQEQKEDVHHSIITDLQN-GDEQTRRRLAMYCLKDAY 501
            |:..|:..........:||.|:.:....|:.::      |:...:| .:..:.....:|.|:..:
Mouse   692 RMICDVEISAKELIHCKSYHLSELVQQILKTER------IVIPTENIRNMYSESSYLLYLLEHIW 750

  Fly   502 LPLRLLEKLMAIVNYMEMA----RVTGVPLESLLTRGQQIKVLSQLLRKAKTKGFIMP------- 555
            ...|.:.::|..:|.:.:|    .:.|..:...|..|:..:....||.......:|:|
Mouse   751 KDARFILQIMCELNVLPLALQITNIAGNIMSRTLMGGRSERNEFLLLHAFYENNYIVPDKQIFRK 815

  Fly   556 -----------------SYTSQGSDEQYEGATVIEPKRGYYADPISTLDFASLYPSIMMAHNLCY 603
                             .|........|.|..|::||.|:|...|..|||.||||||:...|:|:
Mouse   816 PQQKLGDEDEEIDGDTNKYKKGRKKATYAGGLVLDPKVGFYDKFILLLDFNSLYPSIIQEFNICF 880

  Fly   604 TTLVLGGTREKLRQQENLQDDQVERTPANNYFVKSEVRRGLLPEILESLLAARKRAKNDLK-VET 667
            || |...|.|..:..|:.:.:|:...|..|      :..|:||..:..|:..||:.|..:| .:.
Mouse   881 TT-VQRVTSEVQKATEDEEQEQIPELPDPN------LEMGILPREIRKLVERRKQVKQLMKQQDL 938

  Fly   668 DPFKRKVLDGRQLALKISANSVYGFTGAQVGKLPCLEISGSVTAYGRTMIEMTKNEVESHYTQAN 732
            :|......|.||.|||::|||:||..|....:.....::..||..||.::..||:.|:
Mouse   939 NPDLVLQYDIRQKALKLTANSMYGCLGFSYSRFYAKPLAALVTYKGREILMHTKDMVQ------- 996

  Fly   733 GYENNAVVIYGDTDSVMVNFGVKTLERSMELGREAAELVSSKFVHPIKLEFEKVYYPYLLINKKR 797
              :.|..||||||||:|:|.....||...:||.:....| :|....::::.:.|:...||:.||:
Mouse   997 --KMNLEVIYGDTDSIMINTNSTNLEEVFKLGNKVKSEV-NKLYKLLEIDIDAVFKSLLLLKKKK 1058

  Fly   798 YAGLYF--TRPDTY-DKMDCKGIETVRRDNSPLVANLMNSCLQKLLIERDPDGAVAYVKQVIADL 859
            ||.|..  |....| .|.:.||::.||||...|..:..|..:.::|.::..|..|..:::.:.::
Mouse  1059 YAALVVEPTSDGNYITKQELKGLDIVRRDWCDLAKDTGNFVIGQILSDQSRDTIVENIQKRLIEI 1123

  Fly   860 ----LCNRIDISHLVITKELAK--TDYAAKQA--HVELAAKMKKRDPGTAPKLGDRVPYVICAAA 916
                |...:.:|...|.|.|.|  .||..:::  ||.:|..:..:. |...|.||.|.||||...
Mouse  1124 GENVLNGSVPVSQFEINKALTKDPQDYPDRKSLPHVHVALWINSQG-GRKVKAGDTVSYVICQDG 1187

  Fly   917 KNTPAYQKAEDP--LYVLENSVPIDATYYLEQQLSKPLLRIFEPILGDNAESIL----LKGEHTR 975
            .|..|.|:|..|  |..|:| :.||..|||.||:...:.||.|||  |..:::|    |..:.|:
Mouse  1188 SNLTATQRAYAPEQLQKLDN-LAIDTQYYLAQQIHPVVARICEPI--DGIDAVLIALWLGLDSTQ 1249

  Fly   976 TRTVVTSK-------VGGLAGFMTKKTSCLGCKSLMPKGYEQACLCPHC--EPRMSELYQKEVGA 1031
            .|.....|       :||.|. :|.:.....|:..       .||||.|  |.....:::   |:
Mouse  1250 FRVHQYHKDEENDALLGGPAQ-LTDEEKYKDCEKF-------KCLCPSCGTENIYDNVFE---GS 1303

  Fly  1032 KRELEETFSRLWTECQRCQESLHEEVICSNRDC---PIFYMRQ---KVRMDL 1077
            ..::|.:..|                 |||.||   |:.:|.|   |:.||:
Mouse  1304 GLDMEPSLYR-----------------CSNVDCKVSPLTFMVQLSNKLIMDI 1338

Known Domains:

Indicated by green bases in alignment.

Gene	Sequence	Domain	Region	External ID	Identity
DNApol-delta	NP_524099.2	PTZ00166	48..1092	CDD:240301	281/1186 (24%)
		DNA_polB_delta_exo	288..517	CDD:99820	51/248 (21%)
		POLBc_delta	564..960	CDD:99916	134/409 (33%)
		zf-C4pol	997..1070	CDD:290959	16/77 (21%)
Pola1	NP_032918.1	Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	20..39
		DNA_pol_alpha_N	41..101	CDD:289058
		pol2	56..1229	CDD:273159	258/1081 (24%)
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	105..135
		Disordered. /evidence=ECO:0000256\|SAM:MobiDB-lite	261..297		9/41 (22%)
		DNA_polB_alpha_exo	539..771	CDD:99819	52/249 (21%)
		DNA-binding. /evidence=ECO:0000255	654..719		17/72 (24%)
		POLBc_alpha	836..1246	CDD:99915	138/430 (32%)
		DNA-binding. /evidence=ECO:0000255	1249..1380		27/118 (23%)
		zf-DNA_Pol	1269..1458	CDD:286132	23/98 (23%)
		CysB motif	1352..1378
Blue background indicates that the domain is not in the aligned region.

Information from Original Tools:

Tool	Simple Score	Weighted Score	Original Tool Information
Tool	Simple Score	Weighted Score	BLAST Result	Score	Score Type	Cluster ID
Compara	0	0.000	Not matched by this tool.
Domainoid	0	0.000	Not matched by this tool.
eggNOG	1	0.900	-	-		E1_COG0417
Hieranoid	0	0.000	Not matched by this tool.
Homologene	0	0.000	Not matched by this tool.
Inparanoid	0	0.000	Not matched by this tool.
Isobase	0	0.000	Not matched by this tool.
OMA	0	0.000	Not matched by this tool.
OrthoDB	0	0.000	Not matched by this tool.
OrthoFinder	0	0.000	Not matched by this tool.
OrthoInspector	0	0.000	Not matched by this tool.
orthoMCL	0	0.000	Not matched by this tool.
Panther	0	0.000	Not matched by this tool.
Phylome	1	0.910	-	-
RoundUp	0	0.000	Not matched by this tool.
SonicParanoid	0	0.000	Not matched by this tool.
SwiftOrtho	1	1.000	-	-
TreeFam	0	0.000	Not matched by this tool.
	3	2.810